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The map label for this gene is gluQ [H]

Identifier: 85375660

GI number: 85375660

Start: 2844451

End: 2845281

Strand: Direct

Name: gluQ [H]

Synonym: ELI_14165

Alternate gene names: 85375660

Gene position: 2844451-2845281 (Clockwise)

Preceding gene: 85375657

Following gene: 85375661

Centisome position: 93.19

GC content: 65.94

Gene sequence:

>831_bases
GTGATCGTGACGCGCTTCGCCCCGAGCCCCAATGGCCCGCTGCACTGGGGCCATGCCTATTCCGCCATCGTGGCGCATGA
CCTGGCGACGCAGGCCGGCGGCAAGTTTCTTTTGCGAATCGAAGACATCGACGGCCCGCGCACCCGGCCCGAGCTGGCCG
ACGAGTTCCGACGCGACCTCGAATGGCTCGGACTGGAGTGGGAAGAGGTCCCGGCGCAATCGACGCGGCTGGAAAGCTAC
CAGTCGGCAGTCGACCGGCTGCTGGACCAAGGCTTGCTCTACCCCTGCACCTGCACCCGCAAGGAGATCGAAGCGCTCGA
GCCGAGGACGGGGCCGGAAGGGTTCGTCTATCCGGGCACCTGCAAGGGGCGCTTGCTCGATCCCGAAAAGCCCGCCGCCC
TGCGCCTCGATACCGACAAGGCGCTGCTATCGGTCGGCGAGGTTCGTTGGGACGATGCCTTGGCTGGGCTGCAGACGGCA
GACCCGCGCCAGTTCGGCGATGTGGTGCTGGTGCGGAAAGACCTGCCCGCAAGCTATCACCTGGCGGCCACGCTCGACGA
TGCCGCCGACGGGGTCACTCTGGTTACGCGCGGGCAGGACCTGTTCTTCGCCAGCCATATCCACCGTCTGCTCCAGCACC
TGCTCGACCTGCCCGTGCCGCGCTGGCACCACCATCCGCTCCTGCTCGACGAATCCGGCAAAAAACTCGCCAAGACCCGC
GGTTCGAAGGCGCTTGCGGATTATCGCCGGTCGGGCGAGAATGGTCGCGATTGGGCCGATAGACTCCGCATAGACCATTG
GCCGTCTGGTATAGAGCTTGGGAGTGCCTAG

Upstream 100 bases:

>100_bases
TCTCCCCGACTTGGCTTCGAATGTGCATTTGCGCGGAAAAGCGTCAAGACGGTTACACGCAGGCTTTCCACAGGGTTGTC
CCGCGCGCTAGGTCTCCGGT

Downstream 100 bases:

>100_bases
ATACCTGACATGACCTATATACTCATAGCCGCCCTCGCGATCCTGATGGTTCTGGTCCTTTACTCGCTCGTTCGAGGCAT
CATAGCTTTCCTCCAGACGA

Product: hypothetical protein

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MIVTRFAPSPNGPLHWGHAYSAIVAHDLATQAGGKFLLRIEDIDGPRTRPELADEFRRDLEWLGLEWEEVPAQSTRLESY
QSAVDRLLDQGLLYPCTCTRKEIEALEPRTGPEGFVYPGTCKGRLLDPEKPAALRLDTDKALLSVGEVRWDDALAGLQTA
DPRQFGDVVLVRKDLPASYHLAATLDDAADGVTLVTRGQDLFFASHIHRLLQHLLDLPVPRWHHHPLLLDESGKKLAKTR
GSKALADYRRSGENGRDWADRLRIDHWPSGIELGSA

Sequences:

>Translated_276_residues
MIVTRFAPSPNGPLHWGHAYSAIVAHDLATQAGGKFLLRIEDIDGPRTRPELADEFRRDLEWLGLEWEEVPAQSTRLESY
QSAVDRLLDQGLLYPCTCTRKEIEALEPRTGPEGFVYPGTCKGRLLDPEKPAALRLDTDKALLSVGEVRWDDALAGLQTA
DPRQFGDVVLVRKDLPASYHLAATLDDAADGVTLVTRGQDLFFASHIHRLLQHLLDLPVPRWHHHPLLLDESGKKLAKTR
GSKALADYRRSGENGRDWADRLRIDHWPSGIELGSA
>Mature_276_residues
MIVTRFAPSPNGPLHWGHAYSAIVAHDLATQAGGKFLLRIEDIDGPRTRPELADEFRRDLEWLGLEWEEVPAQSTRLESY
QSAVDRLLDQGLLYPCTCTRKEIEALEPRTGPEGFVYPGTCKGRLLDPEKPAALRLDTDKALLSVGEVRWDDALAGLQTA
DPRQFGDVVLVRKDLPASYHLAATLDDAADGVTLVTRGQDLFFASHIHRLLQHLLDLPVPRWHHHPLLLDESGKKLAKTR
GSKALADYRRSGENGRDWADRLRIDHWPSGIELGSA

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=271, Percent_Identity=31.3653136531365, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1788743, Length=256, Percent_Identity=32.03125, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=36.3265306122449, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71993394, Length=268, Percent_Identity=29.1044776119403, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324540, Length=264, Percent_Identity=29.9242424242424, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324742, Length=103, Percent_Identity=31.0679611650485, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665321, Length=273, Percent_Identity=32.967032967033, Blast_Score=120, Evalue=9e-28,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 30826; Mature: 30826

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTRFAPSPNGPLHWGHAYSAIVAHDLATQAGGKFLLRIEDIDGPRTRPELADEFRRDL
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHH
EWLGLEWEEVPAQSTRLESYQSAVDRLLDQGLLYPCTCTRKEIEALEPRTGPEGFVYPGT
HHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEECCC
CKGRLLDPEKPAALRLDTDKALLSVGEVRWDDALAGLQTADPRQFGDVVLVRKDLPASYH
CCCCEECCCCCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCCHHHCCEEEEEECCCCCEE
LAATLDDAADGVTLVTRGQDLFFASHIHRLLQHLLDLPVPRWHHHPLLLDESGKKLAKTR
EEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHC
GSKALADYRRSGENGRDWADRLRIDHWPSGIELGSA
CHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIVTRFAPSPNGPLHWGHAYSAIVAHDLATQAGGKFLLRIEDIDGPRTRPELADEFRRDL
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHH
EWLGLEWEEVPAQSTRLESYQSAVDRLLDQGLLYPCTCTRKEIEALEPRTGPEGFVYPGT
HHHCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEECCC
CKGRLLDPEKPAALRLDTDKALLSVGEVRWDDALAGLQTADPRQFGDVVLVRKDLPASYH
CCCCEECCCCCCEEEECCHHHHHHHHHCCHHHHHHCCCCCCCHHHCCEEEEEECCCCCEE
LAATLDDAADGVTLVTRGQDLFFASHIHRLLQHLLDLPVPRWHHHPLLLDESGKKLAKTR
EEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHC
GSKALADYRRSGENGRDWADRLRIDHWPSGIELGSA
CHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA