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The map label for this gene is dnaB [H]

Identifier: 85375021

GI number: 85375021

Start: 2217149

End: 2218687

Strand: Direct

Name: dnaB [H]

Synonym: ELI_10970

Alternate gene names: 85375021

Gene position: 2217149-2218687 (Clockwise)

Preceding gene: 85375016

Following gene: 85375023

Centisome position: 72.64

GC content: 63.09

Gene sequence:

>1539_bases
ATGGACGATCAGGACCTACTCGTCCGCAGCGATGCGGACAGCGACACGCAAGACGGCGGAGGCAAGGCGCATACCCGCCG
TCTCCCGGCCAATGTCGAGGCCGAGGCGGCGTTTCTAGGCGCAGTCCTGATCGACAACCGCGTGGTCGAAGACCTGCCCA
TTGCGATTCGGCCCGATCATTTCTTCGCATCGATCCACCAGCGCATCTACGACCGCATCCTGACCTTGCTCGATCGCAGT
GCGACGGCGAGTCCGGTTACGCTCAAGCCTTATTTCGACGGCGATGAGGAGCTGAAGGAACTCGGCGGGACAACCTACCT
CGCCAAGCTTACGGCCGATGGGCAAGGCCTCCTCGCCGCGCGCGATCTCGCCCAGCAAATCTACGATCTTGCGTTGTTGC
GCGAGCTGGTGACGGTCGGGCGCACACTGGTGGAGGGCGCGCTCGATACGTCCGACGAAGTCGAGCCGCTGCGGCAGATC
GAACTGGCCGAAAGCTCGCTCTACAAGGTCGCCGAGGGCGCCAGCAGCGGCAGCGAAGCCAAGGATTTCAGGACCGCGGC
GATGACCGCGATCAAGATGGTCGAAGCGGCGATGAATTCGGGCGGCGGCCTGTCGGGCAAGACCAGCGGCATCGCCGTGA
TCGACGACAAGACCGCCGGCCTGCACAATTCGGACCTTATCATCCTCGCCGGGCGTCCGGGCATGGGCAAGAGCTCGCTC
GCCATGAATATCGCGTTCAATTGCGCGGAAGAGCATCTCAAGTTCCAGCGCGATGGCGGTCCGCACAATTACGGCGCGCC
GACAGCCTTCTTCAGCCTGGAAATGAGCGCCGACCAGCTCGCCACGCGTATCCTCGCCGAGCAGGCCGAGATATCCTCCG
AGGCGCTGCGCAGCGGCGACATGGACGCGCGCGATTTCCAGCGGCTGGCCATGGCGAGCCAGAAACTGTCCGAATTGCCG
CTCTATATCGACGACACGCCTGCCCTCACCATTGCCGGGCTGAGAGCGCGCGCGCGGCGGCTCAAGCGGCGGCACGATAT
CGGCTTGATCGTGGTCGACTATCTGCAGCTGCTGCAAGGCTCGGGCCGGGCCAACGACAACCGCGTGAACGAAATTTCGG
AGATCAGCCGGGGCCTCAAAACGCTCGCCAAGGAGCTCGAATGCCCGGTAATCGCGCTCTCGCAGCTCAGCCGTGCGGTC
GAGCAACGCGAAGACAAGCGCCCGATGCTGTCCGACCTGCGTGAATCCGGCTCGATCGAGCAGGATGCCGACATGGTCTG
GTTCATCTACCGCGCCGACTATTACCACGAGGCGATACGGCCCGACATGCCCAATCCGGACGGGTCCAGTTCCCCGGACG
TGGAGGAAAAATATCGCATCTGGGAAGAGCGCTATCTCGAGCTCAAGAACAAGGCGACGCTGATGGTCGCCAAGCAGCGC
CACGGCTCGACCGGCAATGTACCGCTGCATTTCCAGAGCGAATTCACCAAGTTCACCTCGCCCGTGATGAAGGACTATTC
GAGCTGGGGCGAGGGTTAG

Upstream 100 bases:

>100_bases
CGGGCCAAGCAAGCCGCTTGCGCACTCTTCCCCACTATCCACATGACGATGATGCAAAGCGGCACTTGCGCATGTCTGCC
AGCATCCGCAAACAGGTGCG

Downstream 100 bases:

>100_bases
GTCAGCTTACGGCGCACCTTCGCTGCCGACATTGAGCGTGCTGCTGACGGTGTAATCGACCGTGGTCACCAGGAACCACC
CGAAATAGCTCTTCAGGGTC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MDDQDLLVRSDADSDTQDGGGKAHTRRLPANVEAEAAFLGAVLIDNRVVEDLPIAIRPDHFFASIHQRIYDRILTLLDRS
ATASPVTLKPYFDGDEELKELGGTTYLAKLTADGQGLLAARDLAQQIYDLALLRELVTVGRTLVEGALDTSDEVEPLRQI
ELAESSLYKVAEGASSGSEAKDFRTAAMTAIKMVEAAMNSGGGLSGKTSGIAVIDDKTAGLHNSDLIILAGRPGMGKSSL
AMNIAFNCAEEHLKFQRDGGPHNYGAPTAFFSLEMSADQLATRILAEQAEISSEALRSGDMDARDFQRLAMASQKLSELP
LYIDDTPALTIAGLRARARRLKRRHDIGLIVVDYLQLLQGSGRANDNRVNEISEISRGLKTLAKELECPVIALSQLSRAV
EQREDKRPMLSDLRESGSIEQDADMVWFIYRADYYHEAIRPDMPNPDGSSSPDVEEKYRIWEERYLELKNKATLMVAKQR
HGSTGNVPLHFQSEFTKFTSPVMKDYSSWGEG

Sequences:

>Translated_512_residues
MDDQDLLVRSDADSDTQDGGGKAHTRRLPANVEAEAAFLGAVLIDNRVVEDLPIAIRPDHFFASIHQRIYDRILTLLDRS
ATASPVTLKPYFDGDEELKELGGTTYLAKLTADGQGLLAARDLAQQIYDLALLRELVTVGRTLVEGALDTSDEVEPLRQI
ELAESSLYKVAEGASSGSEAKDFRTAAMTAIKMVEAAMNSGGGLSGKTSGIAVIDDKTAGLHNSDLIILAGRPGMGKSSL
AMNIAFNCAEEHLKFQRDGGPHNYGAPTAFFSLEMSADQLATRILAEQAEISSEALRSGDMDARDFQRLAMASQKLSELP
LYIDDTPALTIAGLRARARRLKRRHDIGLIVVDYLQLLQGSGRANDNRVNEISEISRGLKTLAKELECPVIALSQLSRAV
EQREDKRPMLSDLRESGSIEQDADMVWFIYRADYYHEAIRPDMPNPDGSSSPDVEEKYRIWEERYLELKNKATLMVAKQR
HGSTGNVPLHFQSEFTKFTSPVMKDYSSWGEG
>Mature_512_residues
MDDQDLLVRSDADSDTQDGGGKAHTRRLPANVEAEAAFLGAVLIDNRVVEDLPIAIRPDHFFASIHQRIYDRILTLLDRS
ATASPVTLKPYFDGDEELKELGGTTYLAKLTADGQGLLAARDLAQQIYDLALLRELVTVGRTLVEGALDTSDEVEPLRQI
ELAESSLYKVAEGASSGSEAKDFRTAAMTAIKMVEAAMNSGGGLSGKTSGIAVIDDKTAGLHNSDLIILAGRPGMGKSSL
AMNIAFNCAEEHLKFQRDGGPHNYGAPTAFFSLEMSADQLATRILAEQAEISSEALRSGDMDARDFQRLAMASQKLSELP
LYIDDTPALTIAGLRARARRLKRRHDIGLIVVDYLQLLQGSGRANDNRVNEISEISRGLKTLAKELECPVIALSQLSRAV
EQREDKRPMLSDLRESGSIEQDADMVWFIYRADYYHEAIRPDMPNPDGSSSPDVEEKYRIWEERYLELKNKATLMVAKQR
HGSTGNVPLHFQSEFTKFTSPVMKDYSSWGEG

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=478, Percent_Identity=35.7740585774059, Blast_Score=270, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 56338; Mature: 56338

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDQDLLVRSDADSDTQDGGGKAHTRRLPANVEAEAAFLGAVLIDNRVVEDLPIAIRPDH
CCCCCCEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCHHHHCCCCEECCHH
FFASIHQRIYDRILTLLDRSATASPVTLKPYFDGDEELKELGGTTYLAKLTADGQGLLAA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCEEEEEEECCCCCHHHH
RDLAQQIYDLALLRELVTVGRTLVEGALDTSDEVEPLRQIELAESSLYKVAEGASSGSEA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
KDFRTAAMTAIKMVEAAMNSGGGLSGKTSGIAVIDDKTAGLHNSDLIILAGRPGMGKSSL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCE
AMNIAFNCAEEHLKFQRDGGPHNYGAPTAFFSLEMSADQLATRILAEQAEISSEALRSGD
EEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
MDARDFQRLAMASQKLSELPLYIDDTPALTIAGLRARARRLKRRHDIGLIVVDYLQLLQG
CCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
SGRANDNRVNEISEISRGLKTLAKELECPVIALSQLSRAVEQREDKRPMLSDLRESGSIE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
QDADMVWFIYRADYYHEAIRPDMPNPDGSSSPDVEEKYRIWEERYLELKNKATLMVAKQR
CCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCC
HGSTGNVPLHFQSEFTKFTSPVMKDYSSWGEG
CCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDDQDLLVRSDADSDTQDGGGKAHTRRLPANVEAEAAFLGAVLIDNRVVEDLPIAIRPDH
CCCCCCEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCHHHHCCCCEECCHH
FFASIHQRIYDRILTLLDRSATASPVTLKPYFDGDEELKELGGTTYLAKLTADGQGLLAA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHCCCCEEEEEEECCCCCHHHH
RDLAQQIYDLALLRELVTVGRTLVEGALDTSDEVEPLRQIELAESSLYKVAEGASSGSEA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
KDFRTAAMTAIKMVEAAMNSGGGLSGKTSGIAVIDDKTAGLHNSDLIILAGRPGMGKSSL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCE
AMNIAFNCAEEHLKFQRDGGPHNYGAPTAFFSLEMSADQLATRILAEQAEISSEALRSGD
EEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
MDARDFQRLAMASQKLSELPLYIDDTPALTIAGLRARARRLKRRHDIGLIVVDYLQLLQG
CCHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
SGRANDNRVNEISEISRGLKTLAKELECPVIALSQLSRAVEQREDKRPMLSDLRESGSIE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
QDADMVWFIYRADYYHEAIRPDMPNPDGSSSPDVEEKYRIWEERYLELKNKATLMVAKQR
CCCCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCC
HGSTGNVPLHFQSEFTKFTSPVMKDYSSWGEG
CCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA