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The map label for this gene is fabG [C]

Identifier: 85374973

GI number: 85374973

Start: 2175431

End: 2176333

Strand: Direct

Name: fabG [C]

Synonym: ELI_10730

Alternate gene names: 85374973

Gene position: 2175431-2176333 (Clockwise)

Preceding gene: 85374972

Following gene: 85374974

Centisome position: 71.27

GC content: 60.35

Gene sequence:

>903_bases
ATGGACGAAGAGCTTCGTTTCGATGGCAGGGTAGTGGTTGTGACCGGAGCCGGAGGCGGACTTGGGCGCCAGTACGCGCT
GATGTTTGGCGGGCGCGGAGCCAAGGTGGTGGTGAATGATCTGGGTGGCGACGAGAAAGGGAGCGGCAGCTGCTCCACAG
CCGCTGACAATGTGGTCGACGAAATTCGAGCCACGGGCGGTCAGGCGGTGGCAAATTATGCGTCGGTCGAGGAGGGCGCG
CTGATCATCCAAGCCGCGGTTGACAACTTCGGAACCGTTGACGTCGTCATCAACAATGCCGGCATCCTGCGCGATGTGAG
CTTCCACAAAATGACCGACGAAGACTGGACACTGCTTCAGCGGGTCCATCTGAATGGGACGCGGGCAGTGACGCAGGCAG
CATGGCCGATACTGCGCGACAAGGGCTATGGCCGCATCGTCATGACCACGTCTGCAGCCGCAATCTACGGCAACTTCGGT
CAGGCCAACTATGCCGCAGCCAAGCTCGGAATATTGGGGCTTGCGAACGCGCTGGCGGAGGAGGGGCGGTCCAGAAACAT
CCAGGTTAATACGATCGCCCCAATCGCCGCATCGCGTATGACCGCGACTGCCTTTCCACAAGAGTTTCTCGCCAATCTCA
GAGCGGAAGCGATCAGTCCATTGGTCGGTTGGCTTGCGCACGAGAATTGCAGCGAAACCAAAGGGCTTTTTGAAGTTGGA
GCCGGCTATATCGCCAAGCTTAGGTGGGAGCGCGCGCTTGGGCACGCCTTCGGAGCGGACAGGGCGTTCACGCTCGATGA
TGTCGCCCGGCACTGGGGTAGAATTGTCGACTTCACGGATGCCGAGCACCCGCTCGGGCTGAATGACAGCCTTGAGGCGG
TAGAACGGGCGCTGAGAGCGTAA

Upstream 100 bases:

>100_bases
AGACGAAAACGACAGCGTCGACGGAAATGGAGCCGCGTGCCTGACTCGCACAGGATCAAATGCGCAGATGCGTCGCTCAG
GTAAAAGAGGCAGACAAGCG

Downstream 100 bases:

>100_bases
TCAACCCGATGAATTACCATGATATTTTTGAGCGGCCGTCGGGCTGCCGATGGCGTCGTGCTGCCGCCAGATATCGATGA
GCAGCAGGAATAGTTCTGCA

Product: oxidoreductase, short chain dehydrogenase/reductase family protein

Products: estrone; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDEELRFDGRVVVVTGAGGGLGRQYALMFGGRGAKVVVNDLGGDEKGSGSCSTAADNVVDEIRATGGQAVANYASVEEGA
LIIQAAVDNFGTVDVVINNAGILRDVSFHKMTDEDWTLLQRVHLNGTRAVTQAAWPILRDKGYGRIVMTTSAAAIYGNFG
QANYAAAKLGILGLANALAEEGRSRNIQVNTIAPIAASRMTATAFPQEFLANLRAEAISPLVGWLAHENCSETKGLFEVG
AGYIAKLRWERALGHAFGADRAFTLDDVARHWGRIVDFTDAEHPLGLNDSLEAVERALRA

Sequences:

>Translated_300_residues
MDEELRFDGRVVVVTGAGGGLGRQYALMFGGRGAKVVVNDLGGDEKGSGSCSTAADNVVDEIRATGGQAVANYASVEEGA
LIIQAAVDNFGTVDVVINNAGILRDVSFHKMTDEDWTLLQRVHLNGTRAVTQAAWPILRDKGYGRIVMTTSAAAIYGNFG
QANYAAAKLGILGLANALAEEGRSRNIQVNTIAPIAASRMTATAFPQEFLANLRAEAISPLVGWLAHENCSETKGLFEVG
AGYIAKLRWERALGHAFGADRAFTLDDVARHWGRIVDFTDAEHPLGLNDSLEAVERALRA
>Mature_300_residues
MDEELRFDGRVVVVTGAGGGLGRQYALMFGGRGAKVVVNDLGGDEKGSGSCSTAADNVVDEIRATGGQAVANYASVEEGA
LIIQAAVDNFGTVDVVINNAGILRDVSFHKMTDEDWTLLQRVHLNGTRAVTQAAWPILRDKGYGRIVMTTSAAAIYGNFG
QANYAAAKLGILGLANALAEEGRSRNIQVNTIAPIAASRMTATAFPQEFLANLRAEAISPLVGWLAHENCSETKGLFEVG
AGYIAKLRWERALGHAFGADRAFTLDDVARHWGRIVDFTDAEHPLGLNDSLEAVERALRA

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=299, Percent_Identity=58.1939799331104, Blast_Score=351, Evalue=4e-97,
Organism=Homo sapiens, GI15277342, Length=247, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40807363, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI25282469, Length=211, Percent_Identity=27.0142180094787, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI40254992, Length=197, Percent_Identity=28.4263959390863, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI4503817, Length=194, Percent_Identity=25.7731958762887, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1787335, Length=193, Percent_Identity=35.7512953367876, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787905, Length=195, Percent_Identity=33.8461538461538, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082100, Length=193, Percent_Identity=30.5699481865285, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787526, Length=245, Percent_Identity=30.2040816326531, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790717, Length=197, Percent_Identity=26.9035532994924, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788459, Length=237, Percent_Identity=28.6919831223629, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789208, Length=194, Percent_Identity=26.2886597938144, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082160, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=279, Percent_Identity=53.0465949820789, Blast_Score=314, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=30.6451612903226, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17555706, Length=213, Percent_Identity=28.169014084507, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI193203117, Length=199, Percent_Identity=28.643216080402, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=217, Percent_Identity=31.7972350230415, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193203115, Length=201, Percent_Identity=29.3532338308458, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71982365, Length=200, Percent_Identity=28, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564282, Length=180, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=200, Percent_Identity=26, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=194, Percent_Identity=28.3505154639175, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=204, Percent_Identity=28.4313725490196, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=306, Percent_Identity=50.6535947712418, Blast_Score=302, Evalue=4e-83,
Organism=Drosophila melanogaster, GI18859837, Length=284, Percent_Identity=50, Blast_Score=287, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=29.3814432989691, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24639444, Length=241, Percent_Identity=29.4605809128631, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356301, Length=192, Percent_Identity=30.2083333333333, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21357673, Length=199, Percent_Identity=28.1407035175879, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17737361, Length=216, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.62

Molecular weight: Translated: 31930; Mature: 31930

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEELRFDGRVVVVTGAGGGLGRQYALMFGGRGAKVVVNDLGGDEKGSGSCSTAADNVVD
CCCCEEECCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
EIRATGGQAVANYASVEEGALIIQAAVDNFGTVDVVINNAGILRDVSFHKMTDEDWTLLQ
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCEEEECHHHCCCCCHHHHHH
RVHLNGTRAVTQAAWPILRDKGYGRIVMTTSAAAIYGNFGQANYAAAKLGILGLANALAE
HHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHH
EGRSRNIQVNTIAPIAASRMTATAFPQEFLANLRAEAISPLVGWLAHENCSETKGLFEVG
CCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
AGYIAKLRWERALGHAFGADRAFTLDDVARHWGRIVDFTDAEHPLGLNDSLEAVERALRA
CHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDEELRFDGRVVVVTGAGGGLGRQYALMFGGRGAKVVVNDLGGDEKGSGSCSTAADNVVD
CCCCEEECCEEEEEECCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
EIRATGGQAVANYASVEEGALIIQAAVDNFGTVDVVINNAGILRDVSFHKMTDEDWTLLQ
HHHHCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCEEEECHHHCCCCCHHHHHH
RVHLNGTRAVTQAAWPILRDKGYGRIVMTTSAAAIYGNFGQANYAAAKLGILGLANALAE
HHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHH
EGRSRNIQVNTIAPIAASRMTATAFPQEFLANLRAEAISPLVGWLAHENCSETKGLFEVG
CCCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
AGYIAKLRWERALGHAFGADRAFTLDDVARHWGRIVDFTDAEHPLGLNDSLEAVERALRA
CHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: estradiol-17beta; NAD+; NADP+

Specific reaction: estradiol-17beta + NAD(P)+ = estrone + NAD(P)H + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]