BacMap

Definition	Erythrobacter litoralis HTCC2594 chromosome, complete genome.
Accession	NC_007722
Length	3,052,398

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The map label for this gene is paaF [C]

Identifier: 85374970

GI number: 85374970

Start: 2172066

End: 2172860

Strand: Direct

Name: paaF [C]

Synonym: ELI_10715

Alternate gene names: 85374970

Gene position: 2172066-2172860 (Clockwise)

Preceding gene: 85374969

Following gene: 85374971

Centisome position: 71.16

GC content: 60.75

Gene sequence:

>795_bases
ATGGATTATGAATACATCCTGGTTGAGCAACGCGGCCCAGCGCTTTGGGTGACCCTCAACCGGCCGGCAGCGTTGAACAG
TCTGTCGTTGGCTTTGGCCGAGGAACTCGCCGCAGCGATCGAGGCAGCGGAAGCAGATGCGACGGTGCGCGCTTGCATTC
TTACCGGCGCCGGACGGGCGTTTTGCGCTGGCGCGGATCTGCTCGCAATTGGCGATGGAACAGGCGGACAAACTCTCGCC
GAACGCTTAAATCTGTTTCTGCCGCCGCTCGGTCAGGCTTTTAACAGGATCGAGACGTCGCGCCTTCCGATAATTGCGGC
GGTCAACGGCCTTACGCTCGCCGGCGGCTTGGAACTGGTGTTGTGCTGTGATCTCGTCATTGCCGCGCACCCGGCCAGAT
TTGGCGACGCCCATGCAAATTACGGCCTGTTGCCCGGCGGTGGAGGGTCGGTTCGACTCCCTAGGAAGATCGGCGAAGCG
CGTGCGAAACATCTGATGATGACCGGCGGGACCATCTCGGCTGCCGAGATGAAGGAAGCCGGACTCGTGACCCAACTGGT
TGCTCCCGAGGATCTCATTGCGGCAGCGCAAGCGCTGGTAGAATCTCTTGCAGAAAAGAGCCGGCCAGGGCTCGCCTACA
TGAAGCAGCTAGTCCGCGCTGCGCAGGATAGCTCGCTCGAAGTCGGGCTGCGTCAGGAACTCGAAACCATGGCTGCTTAC
GCCTTGTCCAACGACATTGTCGAAGGACTGGCGGCATTCCGGGAGAAGCGGAAGCCCGATTTTTCCGACCGGTGA

Upstream 100 bases:

>100_bases
GGCGGGCCAGGACCGGCGCATTTCGGGGGCATGACCGACGCAGCCATCCTAGCCACATCCTCTATTCCGGTGACCGCCTG
CCGGCGCGCGGGAGACTGCA

Downstream 100 bases:

>100_bases
GACCTGATCGGATGCGGAACACATTGGAAGAATGGATCAACGAATGACCGACATATTCGTCATGGGGGTTGGGATGACCC
CGTTCGGAAAACAGTTCGAC

Product: enoyl CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDYEYILVEQRGPALWVTLNRPAALNSLSLALAEELAAAIEAAEADATVRACILTGAGRAFCAGADLLAIGDGTGGQTLA
ERLNLFLPPLGQAFNRIETSRLPIIAAVNGLTLAGGLELVLCCDLVIAAHPARFGDAHANYGLLPGGGGSVRLPRKIGEA
RAKHLMMTGGTISAAEMKEAGLVTQLVAPEDLIAAAQALVESLAEKSRPGLAYMKQLVRAAQDSSLEVGLRQELETMAAY
ALSNDIVEGLAAFREKRKPDFSDR

Sequences:

>Translated_264_residues
MDYEYILVEQRGPALWVTLNRPAALNSLSLALAEELAAAIEAAEADATVRACILTGAGRAFCAGADLLAIGDGTGGQTLA
ERLNLFLPPLGQAFNRIETSRLPIIAAVNGLTLAGGLELVLCCDLVIAAHPARFGDAHANYGLLPGGGGSVRLPRKIGEA
RAKHLMMTGGTISAAEMKEAGLVTQLVAPEDLIAAAQALVESLAEKSRPGLAYMKQLVRAAQDSSLEVGLRQELETMAAY
ALSNDIVEGLAAFREKRKPDFSDR
>Mature_264_residues
MDYEYILVEQRGPALWVTLNRPAALNSLSLALAEELAAAIEAAEADATVRACILTGAGRAFCAGADLLAIGDGTGGQTLA
ERLNLFLPPLGQAFNRIETSRLPIIAAVNGLTLAGGLELVLCCDLVIAAHPARFGDAHANYGLLPGGGGSVRLPRKIGEA
RAKHLMMTGGTISAAEMKEAGLVTQLVAPEDLIAAAQALVESLAEKSRPGLAYMKQLVRAAQDSSLEVGLRQELETMAAY
ALSNDIVEGLAAFREKRKPDFSDR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=266, Percent_Identity=35.7142857142857, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=33.2046332046332, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=28.1124497991968, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI20127408, Length=243, Percent_Identity=31.6872427983539, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI70995211, Length=270, Percent_Identity=28.5185185185185, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=29.8429319371728, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=29.8429319371728, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI150378541, Length=258, Percent_Identity=31.7829457364341, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI25453481, Length=266, Percent_Identity=24.4360902255639, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI51538573, Length=266, Percent_Identity=24.4360902255639, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4757968, Length=266, Percent_Identity=24.4360902255639, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI49355787, Length=266, Percent_Identity=24.4360902255639, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI221136756, Length=242, Percent_Identity=25.6198347107438, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221136753, Length=242, Percent_Identity=25.6198347107438, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=31.25, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221307494, Length=242, Percent_Identity=25.6198347107438, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4757966, Length=242, Percent_Identity=25.2066115702479, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=242, Percent_Identity=25.2066115702479, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=257, Percent_Identity=22.9571984435798, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI45643119, Length=257, Percent_Identity=22.9571984435798, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI260275230, Length=257, Percent_Identity=22.9571984435798, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI116235487, Length=208, Percent_Identity=28.3653846153846, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=39.9239543726236, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=37.5478927203065, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=30.4511278195489, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=31.7460317460317, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=32.9896907216495, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=27.4436090225564, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=241, Percent_Identity=31.5352697095436, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=35.9375, Blast_Score=153, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=34.1463414634146, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17540714, Length=172, Percent_Identity=35.4651162790698, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=256, Percent_Identity=30.078125, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=256, Percent_Identity=29.296875, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=26.6932270916335, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=26.2962962962963, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=25.1968503937008, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=190, Percent_Identity=32.6315789473684, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=190, Percent_Identity=32.6315789473684, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=190, Percent_Identity=32.6315789473684, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=201, Percent_Identity=30.8457711442786, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=201, Percent_Identity=30.8457711442786, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=30.1075268817204, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=226, Percent_Identity=26.1061946902655, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129971, Length=265, Percent_Identity=35.8490566037736, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24653477, Length=265, Percent_Identity=35.8490566037736, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=35.0597609561753, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=27.6264591439689, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=26.0536398467433, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24650670, Length=255, Percent_Identity=30.1960784313725, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19920382, Length=273, Percent_Identity=26.007326007326, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=217, Percent_Identity=23.963133640553, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921000, Length=195, Percent_Identity=36.4102564102564, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24583077, Length=195, Percent_Identity=36.4102564102564, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583079, Length=195, Percent_Identity=36.4102564102564, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27704; Mature: 27704

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYEYILVEQRGPALWVTLNRPAALNSLSLALAEELAAAIEAAEADATVRACILTGAGRA
CCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCC
FCAGADLLAIGDGTGGQTLAERLNLFLPPLGQAFNRIETSRLPIIAAVNGLTLAGGLELV
EECCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCEEECCHHHHH
LCCDLVIAAHPARFGDAHANYGLLPGGGGSVRLPRKIGEARAKHLMMTGGTISAAEMKEA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHEEEEECCCCCHHHHHHH
GLVTQLVAPEDLIAAAQALVESLAEKSRPGLAYMKQLVRAAQDSSLEVGLRQELETMAAY
CHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALSNDIVEGLAAFREKRKPDFSDR
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYEYILVEQRGPALWVTLNRPAALNSLSLALAEELAAAIEAAEADATVRACILTGAGRA
CCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCC
FCAGADLLAIGDGTGGQTLAERLNLFLPPLGQAFNRIETSRLPIIAAVNGLTLAGGLELV
EECCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCEEECCHHHHH
LCCDLVIAAHPARFGDAHANYGLLPGGGGSVRLPRKIGEARAKHLMMTGGTISAAEMKEA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHEEEEECCCCCHHHHHHH
GLVTQLVAPEDLIAAAQALVESLAEKSRPGLAYMKQLVRAAQDSSLEVGLRQELETMAAY
CHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALSNDIVEGLAAFREKRKPDFSDR
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA