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The map label for this gene is fadA [C]

Identifier: 85374971

GI number: 85374971

Start: 2172904

End: 2174142

Strand: Direct

Name: fadA [C]

Synonym: ELI_10720

Alternate gene names: 85374971

Gene position: 2172904-2174142 (Clockwise)

Preceding gene: 85374970

Following gene: 85374972

Centisome position: 71.19

GC content: 62.87

Gene sequence:

>1239_bases
ATGACCGACATATTCGTCATGGGGGTTGGGATGACCCCGTTCGGAAAACAGTTCGACAAGAGCCTGAAAATGCTCTGCGC
CGAAGCTTCGTCGCAGGCCTTTTCCGACGCAGGATGCACCGCCGGCGATGTCGAAGCCATCTTCTTCGGCAATTGCGTCC
AGGGGCATATGGAGGGCCAGGACATGATCCGCGGCGAAATCGTGGCGCGAGCGATGGGCATCTCAAGCGTTCCCGTGGTG
AATGTCGAAAACGCATGTGCGACGGCTTCCACGGCCTTGCACATGGCCGCCGCCTATGTTCGATCGGGGGCAGCGGATGT
CGTACTCGCGCTCGGCGCGGAGAAGATGTACTCGCCCGACAAGGCGCTCATGTTCAGTGCATTCGATGGCGCTTGGGACG
TGCATGAAACCGAGTCGGGCCGCGCGCAGCTGCTGGCCATGGGCAATGGCGTCGATGTTCCGGAAGGAACGACATCGAGT
CAGCCTTACAGCGTTTTCATGGATGTATACGCCGCGATGGCACGCGCGCACATGAAGACCTACGGCTCGACGCAAGCGCA
AATCGCGGCCGTCTCGGCGAAAAATCACATGCATTCGACGCGCAATCTGCTTGCCCAGTATCGCCTCCCATATACCGTCG
AAAGTGTACTCGCTGCGCCTCCGATCGTTTACCCGTTCACACTGCCGATGTGCTCCCCGATCAGCGACGGCGCCGCCGCT
GCGATCATCTGCAGCGAAGCCGGTCGGCGCAAACTGTCGGCAGCCCCGGACAGGGCGCTAAGAATCCTGGCGTCTGTGCT
GCAAACCGGCGCCGCGCGCGATCCCTTTGATTATGACCACCATGTCAGCCGCCTTGCGGCGAACCGGGCCTATGCGATGG
CCGGCGTGGGGCCAGAAGATATTGGCGTCGCGGAGGTGCATGACGCCACGGCGGTTGGCGAGATCATTGAAATCGAGGCG
CTCGGCCTGACGCCGCCTGGCGAGGGCGGACTCGCGGCAGAGCGCGGTGAGACGGCGCTGGGTGGGCGTATTCCGGTAAA
CACGTCGGGCGGGCTTGAATGCAAGGGACATCCGATCGGCGCCACCGGGCTCGGCCAAATCTACGAGCTTGCGCTGCAAT
TGCGCGGCGAGGCGGAAGATCGACAGGTTCCGAATGCCCGGTTCGCGATCGCGCAGAACGGCGGAGGTGTCATCGGGGTG
GAAGAGGCGGTGGTCGCCGTCACGATCCTGGGCCGGTAG

Upstream 100 bases:

>100_bases
GTCGAAGGACTGGCGGCATTCCGGGAGAAGCGGAAGCCCGATTTTTCCGACCGGTGAGACCTGATCGGATGCGGAACACA
TTGGAAGAATGGATCAACGA

Downstream 100 bases:

>100_bases
TTGTCAGAGCGCGCCTAGGGAAACACGGGGTCACGATATGAACTTTGAGCTGTCCGATGAACAACGCATGGCGGTGGAAA
CCGTCCGCCGCTTTCTCGAC

Product: thiolase

Products: CoA; oxoacyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTDIFVMGVGMTPFGKQFDKSLKMLCAEASSQAFSDAGCTAGDVEAIFFGNCVQGHMEGQDMIRGEIVARAMGISSVPVV
NVENACATASTALHMAAAYVRSGAADVVLALGAEKMYSPDKALMFSAFDGAWDVHETESGRAQLLAMGNGVDVPEGTTSS
QPYSVFMDVYAAMARAHMKTYGSTQAQIAAVSAKNHMHSTRNLLAQYRLPYTVESVLAAPPIVYPFTLPMCSPISDGAAA
AIICSEAGRRKLSAAPDRALRILASVLQTGAARDPFDYDHHVSRLAANRAYAMAGVGPEDIGVAEVHDATAVGEIIEIEA
LGLTPPGEGGLAAERGETALGGRIPVNTSGGLECKGHPIGATGLGQIYELALQLRGEAEDRQVPNARFAIAQNGGGVIGV
EEAVVAVTILGR

Sequences:

>Translated_412_residues
MTDIFVMGVGMTPFGKQFDKSLKMLCAEASSQAFSDAGCTAGDVEAIFFGNCVQGHMEGQDMIRGEIVARAMGISSVPVV
NVENACATASTALHMAAAYVRSGAADVVLALGAEKMYSPDKALMFSAFDGAWDVHETESGRAQLLAMGNGVDVPEGTTSS
QPYSVFMDVYAAMARAHMKTYGSTQAQIAAVSAKNHMHSTRNLLAQYRLPYTVESVLAAPPIVYPFTLPMCSPISDGAAA
AIICSEAGRRKLSAAPDRALRILASVLQTGAARDPFDYDHHVSRLAANRAYAMAGVGPEDIGVAEVHDATAVGEIIEIEA
LGLTPPGEGGLAAERGETALGGRIPVNTSGGLECKGHPIGATGLGQIYELALQLRGEAEDRQVPNARFAIAQNGGGVIGV
EEAVVAVTILGR
>Mature_411_residues
TDIFVMGVGMTPFGKQFDKSLKMLCAEASSQAFSDAGCTAGDVEAIFFGNCVQGHMEGQDMIRGEIVARAMGISSVPVVN
VENACATASTALHMAAAYVRSGAADVVLALGAEKMYSPDKALMFSAFDGAWDVHETESGRAQLLAMGNGVDVPEGTTSSQ
PYSVFMDVYAAMARAHMKTYGSTQAQIAAVSAKNHMHSTRNLLAQYRLPYTVESVLAAPPIVYPFTLPMCSPISDGAAAA
IICSEAGRRKLSAAPDRALRILASVLQTGAARDPFDYDHHVSRLAANRAYAMAGVGPEDIGVAEVHDATAVGEIIEIEAL
GLTPPGEGGLAAERGETALGGRIPVNTSGGLECKGHPIGATGLGQIYELALQLRGEAEDRQVPNARFAIAQNGGGVIGVE
EAVVAVTILGR

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI19923233, Length=410, Percent_Identity=36.5853658536585, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI302344760, Length=407, Percent_Identity=35.8722358722359, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI302344767, Length=337, Percent_Identity=38.2789317507418, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI302344762, Length=410, Percent_Identity=33.4146341463415, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI55956775, Length=372, Percent_Identity=31.4516129032258, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17537653, Length=403, Percent_Identity=32.0099255583127, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24585051, Length=412, Percent_Identity=37.1359223300971, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI19921506, Length=412, Percent_Identity=36.1650485436893, Blast_Score=221, Evalue=5e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16 [C]

Molecular weight: Translated: 42877; Mature: 42746

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIFVMGVGMTPFGKQFDKSLKMLCAEASSQAFSDAGCTAGDVEAIFFGNCVQGHMEGQ
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCCH
DMIRGEIVARAMGISSVPVVNVENACATASTALHMAAAYVRSGAADVVLALGAEKMYSPD
HHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCC
KALMFSAFDGAWDVHETESGRAQLLAMGNGVDVPEGTTSSQPYSVFMDVYAAMARAHMKT
HHEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGSTQAQIAAVSAKNHMHSTRNLLAQYRLPYTVESVLAAPPIVYPFTLPMCSPISDGAAA
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEE
AIICSEAGRRKLSAAPDRALRILASVLQTGAARDPFDYDHHVSRLAANRAYAMAGVGPED
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCC
IGVAEVHDATAVGEIIEIEALGLTPPGEGGLAAERGETALGGRIPVNTSGGLECKGHPIG
CCCCHHCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCC
ATGLGQIYELALQLRGEAEDRQVPNARFAIAQNGGGVIGVEEAVVAVTILGR
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEECCCEEEEEEEECC
>Mature Secondary Structure 
TDIFVMGVGMTPFGKQFDKSLKMLCAEASSQAFSDAGCTAGDVEAIFFGNCVQGHMEGQ
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCCH
DMIRGEIVARAMGISSVPVVNVENACATASTALHMAAAYVRSGAADVVLALGAEKMYSPD
HHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCC
KALMFSAFDGAWDVHETESGRAQLLAMGNGVDVPEGTTSSQPYSVFMDVYAAMARAHMKT
HHEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGSTQAQIAAVSAKNHMHSTRNLLAQYRLPYTVESVLAAPPIVYPFTLPMCSPISDGAAA
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEE
AIICSEAGRRKLSAAPDRALRILASVLQTGAARDPFDYDHHVSRLAANRAYAMAGVGPED
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCC
IGVAEVHDATAVGEIIEIEALGLTPPGEGGLAAERGETALGGRIPVNTSGGLECKGHPIG
CCCCHHCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCC
ATGLGQIYELALQLRGEAEDRQVPNARFAIAQNGGGVIGVEEAVVAVTILGR
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEECCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acyl-CoA; acetyl-CoA [C]

Specific reaction: Acyl-CoA + acetyl-CoA = CoA + oxoacyl-CoA [C]

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]