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The map label for this gene is ahpF [H]

Identifier: 85374918

GI number: 85374918

Start: 2120694

End: 2122283

Strand: Direct

Name: ahpF [H]

Synonym: ELI_10455

Alternate gene names: 85374918

Gene position: 2120694-2122283 (Clockwise)

Preceding gene: 85374917

Following gene: 85374920

Centisome position: 69.48

GC content: 64.03

Gene sequence:

>1590_bases
ATGCTCGACGCATCGATGAAGCAGCAGTTGCAGACCTATTTCGCCAATCTGCGCGAGCCGATCGAGCTCGTCGCCACGCT
TGGCGAAGACGACAAGTCGCGCCAGACGCGCGAACTGCTGGAAGAGATCGCCGACCTGCACGATCTCGTCGGCGCGCGCT
TCGACGGTGATGCTGCGCGCACGCCGAGCTTCACGATCCAGCGCGCCGATGACACTAGCCGCGCAGTCACCTTCGCAGGC
CTGCCGATGGGCCACGAGTTCACCTCGCTGGTGTTGGCGCTGCTCTGGGGTGGCGGTCATCCGCCCAAGGTCGAGCAGGA
CGTGCTGGAGCAGATCCGCGCGCTCGAAGGCCCGCTCGATTTCGAGATGTTCTTTTCCCTGAGCTGCCACAACTGCCCCG
ACGTGGTGCAGGCATTGACGCTTATGGCGCTGGAGAATCCGAACATTTCCGCCACGCTGATCGAAGGCGGCACCTTTCAG
GACGAAGTCGACCGCCGCGACGTGATGGCGGTGCCTGCGACCTTCCTCAATGGCGAGCCGTTCTTCAACGGCAAGATGAG
CCTGGAAGAGGTGCTGGCCAAGGTCGATAGCGGCGCCGACGACAAGGCCGCAGCCAGACTCGACGCGAAGGAGCCTTTCG
AGGTCCTGATCGTCGGCGGCGGCCCGGCGGGCGTCGCGGCCGGCATCTACACCGCGCGCAAGGGCTTCACGACCGGCATC
GCCGCCGAGCGGTTCGGCGGGCAGTTGCAGGATACGCTCGGCATCGAGAACCTGCCCGGCACGGCTTATACCGAAGGCCC
CAAGCTCGCCAATGAGCTCGAGAAGCAGGTCGGCGAAAACGCAATCGACCTGATGAATCTGGCCGAAGCGCGCAAGCTGG
TACCAGCTGAAAGCAAGGGCGGCTTGCACAAGGTCGAACTCGCCAATGGCGCGACGCTCAAGGCTCGCAGCCTGATCCTC
GCCACCGGTGCACGCTGGCGCGAGCTGGGCGTCCCGGGCGAGGCCGAATATCGCAACAAGGGCGTCGCCTATTGCCCGCA
CTGCGACGGTCCGCTGTTCAAGGGCAAGCGTGTCGCCGTGATCGGTGGCGGCAATTCCGGCGTCGAAGCGGCCATCGACC
TCGCCAAGATCGTCGAGCATGTCACACTGATCGAATTCGACAGCAAATTGCGCGCCGACGAGGTGTTGCAGGCCAAGCTC
GCCAGCCTGGGCAATGTCGAGCTTATCAAGAACGCCAAGACCACGGAAATCACCGGCACTAACGGCCGCATGAACGGTCT
GGTCTACGAAGACCGCGCAAGCGGCGAGAGCCACACGGTCGAACTGGCTGGCGTCTTCGTCCAGATCGGCCTCGTGCCCA
ATACCGAATGGCTCAAGGACAGCGGCATCGCCCTTAGCGAGTATGGCGAGATCGAGATCGACGAAGAAGGCGCGACCAAC
CTGCCCGGCATCTTCGCCGCGGGAGACTGTACCACCGTCCCCTACAAGCAGATCGTCGTCGCCATGGGTGAAGGATCGAA
GGCCGGGCTGTCGGCGTTCGATTACCTCATCCGCACCGCACCGGCGGAGGAGATTGCCGAGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGCGCGGGCGGCCTGAAGGCCCGGGGCGATCCTCCCCCTCCCTCCCCGTCCCGGGCCTTCCCCCTTTTCAAAGTTCATC
AATCGGAGACCCTCGCCCCC

Downstream 100 bases:

>100_bases
TCGCTGGAAGCCCCTGATTTCGCGAGAATTCATGCGCCATCGCCTTCGGGTGGCGGCGCATTTTCATTGAGGCCGGTCGC
GCATCGCCCATCGATCACAA

Product: alkyl hydroperoxide reductase, subunit f

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MLDASMKQQLQTYFANLREPIELVATLGEDDKSRQTRELLEEIADLHDLVGARFDGDAARTPSFTIQRADDTSRAVTFAG
LPMGHEFTSLVLALLWGGGHPPKVEQDVLEQIRALEGPLDFEMFFSLSCHNCPDVVQALTLMALENPNISATLIEGGTFQ
DEVDRRDVMAVPATFLNGEPFFNGKMSLEEVLAKVDSGADDKAAARLDAKEPFEVLIVGGGPAGVAAGIYTARKGFTTGI
AAERFGGQLQDTLGIENLPGTAYTEGPKLANELEKQVGENAIDLMNLAEARKLVPAESKGGLHKVELANGATLKARSLIL
ATGARWRELGVPGEAEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAKIVEHVTLIEFDSKLRADEVLQAKL
ASLGNVELIKNAKTTEITGTNGRMNGLVYEDRASGESHTVELAGVFVQIGLVPNTEWLKDSGIALSEYGEIEIDEEGATN
LPGIFAAGDCTTVPYKQIVVAMGEGSKAGLSAFDYLIRTAPAEEIAEAA

Sequences:

>Translated_529_residues
MLDASMKQQLQTYFANLREPIELVATLGEDDKSRQTRELLEEIADLHDLVGARFDGDAARTPSFTIQRADDTSRAVTFAG
LPMGHEFTSLVLALLWGGGHPPKVEQDVLEQIRALEGPLDFEMFFSLSCHNCPDVVQALTLMALENPNISATLIEGGTFQ
DEVDRRDVMAVPATFLNGEPFFNGKMSLEEVLAKVDSGADDKAAARLDAKEPFEVLIVGGGPAGVAAGIYTARKGFTTGI
AAERFGGQLQDTLGIENLPGTAYTEGPKLANELEKQVGENAIDLMNLAEARKLVPAESKGGLHKVELANGATLKARSLIL
ATGARWRELGVPGEAEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAKIVEHVTLIEFDSKLRADEVLQAKL
ASLGNVELIKNAKTTEITGTNGRMNGLVYEDRASGESHTVELAGVFVQIGLVPNTEWLKDSGIALSEYGEIEIDEEGATN
LPGIFAAGDCTTVPYKQIVVAMGEGSKAGLSAFDYLIRTAPAEEIAEAA
>Mature_529_residues
MLDASMKQQLQTYFANLREPIELVATLGEDDKSRQTRELLEEIADLHDLVGARFDGDAARTPSFTIQRADDTSRAVTFAG
LPMGHEFTSLVLALLWGGGHPPKVEQDVLEQIRALEGPLDFEMFFSLSCHNCPDVVQALTLMALENPNISATLIEGGTFQ
DEVDRRDVMAVPATFLNGEPFFNGKMSLEEVLAKVDSGADDKAAARLDAKEPFEVLIVGGGPAGVAAGIYTARKGFTTGI
AAERFGGQLQDTLGIENLPGTAYTEGPKLANELEKQVGENAIDLMNLAEARKLVPAESKGGLHKVELANGATLKARSLIL
ATGARWRELGVPGEAEYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAKIVEHVTLIEFDSKLRADEVLQAKL
ASLGNVELIKNAKTTEITGTNGRMNGLVYEDRASGESHTVELAGVFVQIGLVPNTEWLKDSGIALSEYGEIEIDEEGATN
LPGIFAAGDCTTVPYKQIVVAMGEGSKAGLSAFDYLIRTAPAEEIAEAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=299, Percent_Identity=27.4247491638796, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI21389617, Length=299, Percent_Identity=27.4247491638796, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI226437568, Length=299, Percent_Identity=27.4247491638796, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI87081763, Length=523, Percent_Identity=58.6998087954111, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1787114, Length=324, Percent_Identity=32.7160493827161, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17559934, Length=205, Percent_Identity=31.7073170731707, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321898, Length=317, Percent_Identity=32.807570977918, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320560, Length=325, Percent_Identity=31.6923076923077, Blast_Score=130, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56457; Mature: 56457

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDASMKQQLQTYFANLREPIELVATLGEDDKSRQTRELLEEIADLHDLVGARFDGDAAR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TPSFTIQRADDTSRAVTFAGLPMGHEFTSLVLALLWGGGHPPKVEQDVLEQIRALEGPLD
CCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
FEMFFSLSCHNCPDVVQALTLMALENPNISATLIEGGTFQDEVDRRDVMAVPATFLNGEP
EEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCEEECCHHHCCCCC
FFNGKMSLEEVLAKVDSGADDKAAARLDAKEPFEVLIVGGGPAGVAAGIYTARKGFTTGI
CCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCH
AAERFGGQLQDTLGIENLPGTAYTEGPKLANELEKQVGENAIDLMNLAEARKLVPAESKG
HHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
GLHKVELANGATLKARSLILATGARWRELGVPGEAEYRNKGVAYCPHCDGPLFKGKRVAV
CEEEEEECCCCEEECCEEEEECCCCHHHCCCCCCHHHCCCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAKIVEHVTLIEFDSKLRADEVLQAKLASLGNVELIKNAKTTEITGT
EECCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEEECC
NGRMNGLVYEDRASGESHTVELAGVFVQIGLVPNTEWLKDSGIALSEYGEIEIDEEGATN
CCCCCCEEEECCCCCCCCEEEEEEEEEEEEECCCCHHHCCCCCEEECCCCEEECCCCCCC
LPGIFAAGDCTTVPYKQIVVAMGEGSKAGLSAFDYLIRTAPAEEIAEAA
CCCEEECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MLDASMKQQLQTYFANLREPIELVATLGEDDKSRQTRELLEEIADLHDLVGARFDGDAAR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TPSFTIQRADDTSRAVTFAGLPMGHEFTSLVLALLWGGGHPPKVEQDVLEQIRALEGPLD
CCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
FEMFFSLSCHNCPDVVQALTLMALENPNISATLIEGGTFQDEVDRRDVMAVPATFLNGEP
EEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCEEECCHHHCCCCC
FFNGKMSLEEVLAKVDSGADDKAAARLDAKEPFEVLIVGGGPAGVAAGIYTARKGFTTGI
CCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCH
AAERFGGQLQDTLGIENLPGTAYTEGPKLANELEKQVGENAIDLMNLAEARKLVPAESKG
HHHHHCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCC
GLHKVELANGATLKARSLILATGARWRELGVPGEAEYRNKGVAYCPHCDGPLFKGKRVAV
CEEEEEECCCCEEECCEEEEECCCCHHHCCCCCCHHHCCCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAKIVEHVTLIEFDSKLRADEVLQAKLASLGNVELIKNAKTTEITGT
EECCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCEEEECCCCCEEEECC
NGRMNGLVYEDRASGESHTVELAGVFVQIGLVPNTEWLKDSGIALSEYGEIEIDEEGATN
CCCCCCEEEECCCCCCCCEEEEEEEEEEEEECCCCHHHCCCCCEEECCCCEEECCCCCCC
LPGIFAAGDCTTVPYKQIVVAMGEGSKAGLSAFDYLIRTAPAEEIAEAA
CCCEEECCCCCCCCHHHEEEEECCCCCCHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]