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The map label for this gene is pheA [H]

Identifier: 85374885

GI number: 85374885

Start: 2088714

End: 2089607

Strand: Direct

Name: pheA [H]

Synonym: ELI_10290

Alternate gene names: 85374885

Gene position: 2088714-2089607 (Clockwise)

Preceding gene: 85374883

Following gene: 85374886

Centisome position: 68.43

GC content: 65.1

Gene sequence:

>894_bases
ATGTTGAGTTTTCCCGCCCCGGCGCTCGCGATGGTCGAGCGGATGCGCGCAGCCGCCGCTGCCGATCCCGCGCGCGCCAT
CGCCTTGCAGGGTGCACCGGGCGCCAATTCGCACCGCGCGGCGCAGGAATATGCGCCGGAATTGCTGCCCTTTCCCTGCT
TCAGCTTCGAAGACGCGATCGAAGCGGTGCAGACCGGCAAGGCCGGCTGTGCGATCATCCCGATCGAAAACAGCCAGCAC
GGCCGCGTGGCCGATATCCACTTCCTGCTGCCCAACAGCGGGCTGCAAATCGTCGGCGAGCATTTCATGCGGATCGAGCA
CGCGCTGATGGGGCTGGGCAAGGGCCCGTTCGAAGCCGCCTACTCGCATCCGCAGGCGCTGGGCCAATCGCGCGAATTCC
TCCGCAAGCGCGGGATGGTACCGCTGAGCTTTGCGGATACCGCCGGTGCGGCGGCGCATGTTGCCGAGCGGCAGGACGCA
AGCCTCGCCGCCATCGCGCCGGCGCTCGCAGCCGAGCTCTACGGTCTCGATATCGTCGAAGAGAATGTCGAAGACGCGCA
CGACAACACGACGCGCTTCGTCGTGCTGGCCAAGGAAGGTCTCGATCCGGCCACGCTCGCAGGCGAGCAGGCGATGACCA
CCTTCATCTTCACCGTGAAGAACATTCCCGCGGCGCTCTACAAGGCGATGGGAGGTTTCGCGACCAACGGCGTCAACATG
ACCAAGCTGGAAAGCTACCAGCAGGGCGCCAGCTTTGCCGCTTCGACCTTCTATGCCGATATCATGGGCGCGCCGGGCGA
CCCGGCAGTCGACCGTGCTCTGGAAGAACTCGATTTTCATTCGGCCAGACTCAGGATGCTGGGGACATATCGGATGGCCC
GCCAGCGCGGCTAG

Upstream 100 bases:

>100_bases
GGAATTGACCAGAATCTCTCCCCGCGCCCGCCCTTCGTTGCAATCGCGGAAATCATAATTGCTTGCGGCGCAATTTGCGC
GCCGCTACCCGCGCTGGCCA

Downstream 100 bases:

>100_bases
CCATCCGGCTTGCGCGGTCGCTTGGCCCATGTCACCACGGGCATCGTGACAGTGGGTCGGGCCAATCCTGAAAGTGCGAG
CGAGGCGTGGGAAAGCGTGC

Product: prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLSFPAPALAMVERMRAAAAADPARAIALQGAPGANSHRAAQEYAPELLPFPCFSFEDAIEAVQTGKAGCAIIPIENSQH
GRVADIHFLLPNSGLQIVGEHFMRIEHALMGLGKGPFEAAYSHPQALGQSREFLRKRGMVPLSFADTAGAAAHVAERQDA
SLAAIAPALAAELYGLDIVEENVEDAHDNTTRFVVLAKEGLDPATLAGEQAMTTFIFTVKNIPAALYKAMGGFATNGVNM
TKLESYQQGASFAASTFYADIMGAPGDPAVDRALEELDFHSARLRMLGTYRMARQRG

Sequences:

>Translated_297_residues
MLSFPAPALAMVERMRAAAAADPARAIALQGAPGANSHRAAQEYAPELLPFPCFSFEDAIEAVQTGKAGCAIIPIENSQH
GRVADIHFLLPNSGLQIVGEHFMRIEHALMGLGKGPFEAAYSHPQALGQSREFLRKRGMVPLSFADTAGAAAHVAERQDA
SLAAIAPALAAELYGLDIVEENVEDAHDNTTRFVVLAKEGLDPATLAGEQAMTTFIFTVKNIPAALYKAMGGFATNGVNM
TKLESYQQGASFAASTFYADIMGAPGDPAVDRALEELDFHSARLRMLGTYRMARQRG
>Mature_297_residues
MLSFPAPALAMVERMRAAAAADPARAIALQGAPGANSHRAAQEYAPELLPFPCFSFEDAIEAVQTGKAGCAIIPIENSQH
GRVADIHFLLPNSGLQIVGEHFMRIEHALMGLGKGPFEAAYSHPQALGQSREFLRKRGMVPLSFADTAGAAAHVAERQDA
SLAAIAPALAAELYGLDIVEENVEDAHDNTTRFVVLAKEGLDPATLAGEQAMTTFIFTVKNIPAALYKAMGGFATNGVNM
TKLESYQQGASFAASTFYADIMGAPGDPAVDRALEELDFHSARLRMLGTYRMARQRG

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=272, Percent_Identity=30.8823529411765, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 31659; Mature: 31659

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00857 PREPHENATE_DEHYDR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFPAPALAMVERMRAAAAADPARAIALQGAPGANSHRAAQEYAPELLPFPCFSFEDAI
CCCCCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHH
EAVQTGKAGCAIIPIENSQHGRVADIHFLLPNSGLQIVGEHFMRIEHALMGLGKGPFEAA
HHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
YSHPQALGQSREFLRKRGMVPLSFADTAGAAAHVAERQDASLAAIAPALAAELYGLDIVE
HCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
ENVEDAHDNTTRFVVLAKEGLDPATLAGEQAMTTFIFTVKNIPAALYKAMGGFATNGVNM
HHHHHCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
TKLESYQQGASFAASTFYADIMGAPGDPAVDRALEELDFHSARLRMLGTYRMARQRG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSFPAPALAMVERMRAAAAADPARAIALQGAPGANSHRAAQEYAPELLPFPCFSFEDAI
CCCCCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHHH
EAVQTGKAGCAIIPIENSQHGRVADIHFLLPNSGLQIVGEHFMRIEHALMGLGKGPFEAA
HHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
YSHPQALGQSREFLRKRGMVPLSFADTAGAAAHVAERQDASLAAIAPALAAELYGLDIVE
HCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
ENVEDAHDNTTRFVVLAKEGLDPATLAGEQAMTTFIFTVKNIPAALYKAMGGFATNGVNM
HHHHHCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
TKLESYQQGASFAASTFYADIMGAPGDPAVDRALEELDFHSARLRMLGTYRMARQRG
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]