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The map label for this gene is hisH

Identifier: 85374740

GI number: 85374740

Start: 1950214

End: 1950846

Strand: Reverse

Name: hisH

Synonym: ELI_09565

Alternate gene names: 85374740

Gene position: 1950846-1950214 (Counterclockwise)

Preceding gene: 85374741

Following gene: 85374739

Centisome position: 63.91

GC content: 61.93

Gene sequence:

>633_bases
GTGGCTGAGAGGATCGCGCTGATCGACTATGGCGCGGGCAACCTGCATTCAGTTCACAACGCATTGAAGGCTGCCGGAGC
GCACGGGGTCAAAGTTACGCATAATCCCGATCTTGTCCGCATGGCGGATCGCGTGGTGTTGCCGGGTGTCGGTTCTTACA
AGGCTTGTGCCGATGGACTGCGCGGCATTGCGGGAATGGTCGATGCGCTGGAGGAGCGGGTGCTGCTGGGCGGCGTACCA
TTCCTCGGCATTTGCGTGGGCATGCAATTGCTGGCGACCCGCGGGCTGGAACATGCCGAAACGCCGGGGCTCGACTGGGT
GGCGGGCGAAGTGCGGCTGATCGAGCCGACCGATCCGAGCGTCAAGGTGCCTCACATGGGCTGGAACGACGTGGCATTGA
CGCCGCATGCGAAAAACCACGCATTGGTCGACGAGGGCGAAGCGTATTTCCTCCATTCCTATCATTTTCATGCCGATGAG
GGCCGCGACGTCCTAGCGATGACCGACCATGGCGGCGGGCTTGTGGCGGCGGTGGCGCGGGATAATATCGTCGGCGTGCA
ATTTCACCCGGAGAAGAGCCAAGCCTATGGGCTGGGGCTGCTCACCCGTTTTCTCGATTGGAACCCGGCATGA

Upstream 100 bases:

>100_bases
TGCGAGAGCATCTACAAGGGCTTCGCCCGCGCGATGCGGCAGGCGGTGGAACTGGATGGGCGCAAGGGCGGGGCGATCCC
GAGCACCAAGGGGCAGTTGG

Downstream 100 bases:

>100_bases
TCGTCTTTCCCGCCATCGACCTCAAGGGCGGCGAAGTCGTGCGGCTGGCCGAGGGCGATATGGATCGCGCCACCGTCTAT
GGCGACAACCCGGCAGCGCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: AMP; diphosphate; GMP; L-glutamate

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MAERIALIDYGAGNLHSVHNALKAAGAHGVKVTHNPDLVRMADRVVLPGVGSYKACADGLRGIAGMVDALEERVLLGGVP
FLGICVGMQLLATRGLEHAETPGLDWVAGEVRLIEPTDPSVKVPHMGWNDVALTPHAKNHALVDEGEAYFLHSYHFHADE
GRDVLAMTDHGGGLVAAVARDNIVGVQFHPEKSQAYGLGLLTRFLDWNPA

Sequences:

>Translated_210_residues
MAERIALIDYGAGNLHSVHNALKAAGAHGVKVTHNPDLVRMADRVVLPGVGSYKACADGLRGIAGMVDALEERVLLGGVP
FLGICVGMQLLATRGLEHAETPGLDWVAGEVRLIEPTDPSVKVPHMGWNDVALTPHAKNHALVDEGEAYFLHSYHFHADE
GRDVLAMTDHGGGLVAAVARDNIVGVQFHPEKSQAYGLGLLTRFLDWNPA
>Mature_209_residues
AERIALIDYGAGNLHSVHNALKAAGAHGVKVTHNPDLVRMADRVVLPGVGSYKACADGLRGIAGMVDALEERVLLGGVPF
LGICVGMQLLATRGLEHAETPGLDWVAGEVRLIEPTDPSVKVPHMGWNDVALTPHAKNHALVDEGEAYFLHSYHFHADEG
RDVLAMTDHGGGLVAAVARDNIVGVQFHPEKSQAYGLGLLTRFLDWNPA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=207, Percent_Identity=37.1980676328502, Blast_Score=116, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=30.188679245283, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 6.3.5.2

Molecular weight: Translated: 22415; Mature: 22284

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERIALIDYGAGNLHSVHNALKAAGAHGVKVTHNPDLVRMADRVVLPGVGSYKACADGL
CCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCEEECCCCCCHHHHHHHH
RGIAGMVDALEERVLLGGVPFLGICVGMQLLATRGLEHAETPGLDWVAGEVRLIEPTDPS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCC
VKVPHMGWNDVALTPHAKNHALVDEGEAYFLHSYHFHADEGRDVLAMTDHGGGLVAAVAR
EECCCCCCCCEEECCCCCCCCEEECCCEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEC
DNIVGVQFHPEKSQAYGLGLLTRFLDWNPA
CCEEEEEECCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AERIALIDYGAGNLHSVHNALKAAGAHGVKVTHNPDLVRMADRVVLPGVGSYKACADGL
CCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCEEECCCCCCHHHHHHHH
RGIAGMVDALEERVLLGGVPFLGICVGMQLLATRGLEHAETPGLDWVAGEVRLIEPTDPS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEECCCCCC
VKVPHMGWNDVALTPHAKNHALVDEGEAYFLHSYHFHADEGRDVLAMTDHGGGLVAAVAR
EECCCCCCCCEEECCCCCCCCEEECCCEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEC
DNIVGVQFHPEKSQAYGLGLLTRFLDWNPA
CCEEEEEECCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xanthosine 5'-phosphate; L-glutamine; H2O

Specific reaction: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA