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The map label for this gene is ycfF [C]

Identifier: 85374735

GI number: 85374735

Start: 1947429

End: 1947812

Strand: Reverse

Name: ycfF [C]

Synonym: ELI_09540

Alternate gene names: 85374735

Gene position: 1947812-1947429 (Counterclockwise)

Preceding gene: 85374737

Following gene: 85374734

Centisome position: 63.81

GC content: 61.2

Gene sequence:

>384_bases
ATGCCGATTGACCCCACGCTACCCTATGACGACGATAATATCTTCGCCAAGATCCTGCGCGGCGAGATCCCGTCGAACAA
GGTTTATGAGGATGACTGGGCTTTCGCGTTCGAGGACATCAACCCGCAGGCCGAGGTGCACACGCTGGTGATCCCCAAGG
GCCAATATGTCAGCTGGGACGATTTTTCCGCCAAGGCCACGGCGGAGGAGATCGCTGGCTTCGTGCGCGCCGTAGGCGAA
GTGGCGCGGATGAAAGGTCTGGTCGAGCACGGCTACCGCATGATGGCCAATATCGGCGCGCATGGCGGGCAGGAGGTGCC
GCACCTGCATGTCCATATCTTCGGCGGGCATCCGCTGGGGCCGATGATTGCGCGACGAAGGTAG

Upstream 100 bases:

>100_bases
AATACTTCATCTGACTGAGATTAGTGGCCATACTACCCCCCGAGTGAATCTGACATACTGAAGCTCCGAGTATTTGAGTT
TGGAGGAAAAAGAAAGAGTT

Downstream 100 bases:

>100_bases
GGCAGCCCCTCTCAACTCCCACCGTCACCCCGGACTTGATCCGGGGTCCCGCTGTCTTCGGCGCCGCGCTTGAAGAAAGC
GGGACCCCGGCTCGGGGGCC

Product: HIT-like protein

Products: ADP

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MPIDPTLPYDDDNIFAKILRGEIPSNKVYEDDWAFAFEDINPQAEVHTLVIPKGQYVSWDDFSAKATAEEIAGFVRAVGE
VARMKGLVEHGYRMMANIGAHGGQEVPHLHVHIFGGHPLGPMIARRR

Sequences:

>Translated_127_residues
MPIDPTLPYDDDNIFAKILRGEIPSNKVYEDDWAFAFEDINPQAEVHTLVIPKGQYVSWDDFSAKATAEEIAGFVRAVGE
VARMKGLVEHGYRMMANIGAHGGQEVPHLHVHIFGGHPLGPMIARRR
>Mature_126_residues
PIDPTLPYDDDNIFAKILRGEIPSNKVYEDDWAFAFEDINPQAEVHTLVIPKGQYVSWDDFSAKATAEEIAGFVRAVGEV
ARMKGLVEHGYRMMANIGAHGGQEVPHLHVHIFGGHPLGPMIARRR

Specific function: Unknown

COG id: COG0537

COG function: function code FGR; Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HIT domain [H]

Homologues:

Organism=Homo sapiens, GI4885413, Length=108, Percent_Identity=42.5925925925926, Blast_Score=86, Evalue=8e-18,
Organism=Homo sapiens, GI14211923, Length=108, Percent_Identity=37.962962962963, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787346, Length=116, Percent_Identity=43.9655172413793, Blast_Score=103, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17506713, Length=109, Percent_Identity=40.3669724770642, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28574010, Length=118, Percent_Identity=42.3728813559322, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24581222, Length=111, Percent_Identity=40.5405405405405, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011146
- InterPro:   IPR011151
- InterPro:   IPR019808
- InterPro:   IPR001310 [H]

Pfam domain/function: PF01230 HIT [H]

EC number: 3.6.1.41

Molecular weight: Translated: 14164; Mature: 14033

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00892 HIT_1 ; PS51084 HIT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDPTLPYDDDNIFAKILRGEIPSNKVYEDDWAFAFEDINPQAEVHTLVIPKGQYVSWD
CCCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCEEEEEEECCCCEECCC
DFSAKATAEEIAGFVRAVGEVARMKGLVEHGYRMMANIGAHGGQEVPHLHVHIFGGHPLG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
PMIARRR
CHHCCCC
>Mature Secondary Structure 
PIDPTLPYDDDNIFAKILRGEIPSNKVYEDDWAFAFEDINPQAEVHTLVIPKGQYVSWD
CCCCCCCCCCCHHHHHHHHCCCCCCCEECCCCEEEECCCCCCCEEEEEEECCCCEECCC
DFSAKATAEEIAGFVRAVGEVARMKGLVEHGYRMMANIGAHGGQEVPHLHVHIFGGHPLG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
PMIARRR
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: P1,P4-bis(5'-adenosyl) tetraphosphate; H2O

Specific reaction: P1,P4-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2664449; 1472710 [H]