Click here to switch to the map view.

The map label for this gene is purT

Identifier: 85374723

GI number: 85374723

Start: 1939575

End: 1940747

Strand: Direct

Name: purT

Synonym: ELI_09480

Alternate gene names: 85374723

Gene position: 1939575-1940747 (Clockwise)

Preceding gene: 85374722

Following gene: 85374724

Centisome position: 63.54

GC content: 63.34

Gene sequence:

>1173_bases
ATGCCACACACGGCAAAGATCCTGCTGCTCGGCTCGGGCGAGCTCGGACGCGAATTCGTCATCTCGGCCAAGCGGCTGGG
CGCTTACGTGGTGGCCTGCGATGCCTATGCGGCCGCGCCAGCCATGCAGGTGGCGGATGCGAGCGAAGTGCTGTCCATGC
TCGATGCCGACGCGCTGCGCGCAGTGGTAGCGAAACACCATCCCGATTATGTCGTGCCGGAGATCGAGGCGATCCGCACC
GAGGTGCTGGCCGAGATCGAGGCGGATGGCTTCAATGTCGTACCCAGCGCCTATGCCACCCAAATGACGATGAACCGCGA
TGCCATCCGCGACCTTGCCGCGCAGGAACTGGGCATCACCACTTCGCGCTATCGCTATGCCAAGAACCTGGAGGAAGTGC
GCGCCGCCGCCGAGTTCACCGGATACCCCTGCGTCATCAAACCGGTCATGTCCTCCAGCGGCAAGGGCCAGAGCACGGTC
CGCTCTGCCGACAAGCTGGAAGAAGCGTGGGACTATGCCGTCGCCAACATGCGCGGCGACAGGAAGCGCGTGATCGTCGA
GCAGTTCATCGACTTCGATTACGAGATCACGCTCCTCACCGTGCGCCACAAGGACGGTATCACGTTCTGCCCCCCGATTG
GCCACCGCCAGGAGCGCGGCGATTATCGTGAAAGCTGGCAACCCGCGACCATGAGCAAGCCTGCCATTGCAGCAGCGCAG
GAAATGGCGGAGAAAGTCGTCACCGCGCTGCAGGGCAATGGCAAGGGATTTGGGCTGTTCGGCGTGGAATTCTTCGTGAA
GGGTGAGGAAGTCATTTTCTCCGAGCTGAGCCCGCGCCCGCACGATACCGGCATGGTGACCTCGGTCAGCCAGAACCTCA
GCGAATTCGACCTGCATGCGCGCGCCATCATGGGATTGCACGTCCCCTCTGAGATCCTCGCGCGCCCGTCTGCCAGCGCC
GTGATTCTCGCCGAGCAAGAGAGCGAGACAGTCAGCTATTCGGGCCTCGCCGCAGCAATGGAAGGCGGCGCCGACATCCG
CATTTTCGGCAAGCCGAACACCCGCCCCTACCGGCGCATGGGCGTCGCTCTCGCGACGGGCGGCGATACCGATTATGCGC
GCACCGCTGCGGTCGCTGCGGCTAACAAGCTGCACATTCACTACGGAGACTAG

Upstream 100 bases:

>100_bases
TGGCAATCCATGGCCAAACTTCTCAGCAAGTACTGTGTTGCCATGGATCGCCGCGTCGCTACGCTCCTCGCGATGACGAT
TGAATAGGGAAGGCGCTTGA

Downstream 100 bases:

>100_bases
CGTCATGACCAAGCCCGTGTTTCTCGTGATCGGTGCCGGAGCGGGCATCGGCGGCCATGCAGCGAAGCGCTTCGCCAAAG
GCGGCTATCACGCGGTGTTG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPHTAKILLLGSGELGREFVISAKRLGAYVVACDAYAAAPAMQVADASEVLSMLDADALRAVVAKHHPDYVVPEIEAIRT
EVLAEIEADGFNVVPSAYATQMTMNRDAIRDLAAQELGITTSRYRYAKNLEEVRAAAEFTGYPCVIKPVMSSSGKGQSTV
RSADKLEEAWDYAVANMRGDRKRVIVEQFIDFDYEITLLTVRHKDGITFCPPIGHRQERGDYRESWQPATMSKPAIAAAQ
EMAEKVVTALQGNGKGFGLFGVEFFVKGEEVIFSELSPRPHDTGMVTSVSQNLSEFDLHARAIMGLHVPSEILARPSASA
VILAEQESETVSYSGLAAAMEGGADIRIFGKPNTRPYRRMGVALATGGDTDYARTAAVAAANKLHIHYGD

Sequences:

>Translated_390_residues
MPHTAKILLLGSGELGREFVISAKRLGAYVVACDAYAAAPAMQVADASEVLSMLDADALRAVVAKHHPDYVVPEIEAIRT
EVLAEIEADGFNVVPSAYATQMTMNRDAIRDLAAQELGITTSRYRYAKNLEEVRAAAEFTGYPCVIKPVMSSSGKGQSTV
RSADKLEEAWDYAVANMRGDRKRVIVEQFIDFDYEITLLTVRHKDGITFCPPIGHRQERGDYRESWQPATMSKPAIAAAQ
EMAEKVVTALQGNGKGFGLFGVEFFVKGEEVIFSELSPRPHDTGMVTSVSQNLSEFDLHARAIMGLHVPSEILARPSASA
VILAEQESETVSYSGLAAAMEGGADIRIFGKPNTRPYRRMGVALATGGDTDYARTAAVAAANKLHIHYGD
>Mature_389_residues
PHTAKILLLGSGELGREFVISAKRLGAYVVACDAYAAAPAMQVADASEVLSMLDADALRAVVAKHHPDYVVPEIEAIRTE
VLAEIEADGFNVVPSAYATQMTMNRDAIRDLAAQELGITTSRYRYAKNLEEVRAAAEFTGYPCVIKPVMSSSGKGQSTVR
SADKLEEAWDYAVANMRGDRKRVIVEQFIDFDYEITLLTVRHKDGITFCPPIGHRQERGDYRESWQPATMSKPAIAAAQE
MAEKVVTALQGNGKGFGLFGVEFFVKGEEVIFSELSPRPHDTGMVTSVSQNLSEFDLHARAIMGLHVPSEILARPSASAV
ILAEQESETVSYSGLAAAMEGGADIRIFGKPNTRPYRRMGVALATGGDTDYARTAAVAAANKLHIHYGD

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=365, Percent_Identity=56.7123287671233, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1786733, Length=381, Percent_Identity=24.4094488188976, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=371, Percent_Identity=23.989218328841, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_ERYLH (Q2N8K3)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458785.1
- ProteinModelPortal:   Q2N8K3
- SMR:   Q2N8K3
- STRING:   Q2N8K3
- GeneID:   3869947
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_09480
- NMPDR:   fig|314225.3.peg.1141
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q2N8K3
- ProtClustDB:   PRK09288
- BioCyc:   ELIT314225:ELI_09480-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42303; Mature: 42172

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 74-74 BINDING 106-106 BINDING 147-147 BINDING 195-195 BINDING 263-263 BINDING 275-275 BINDING 282-282 BINDING 351-351

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHTAKILLLGSGELGREFVISAKRLGAYVVACDAYAAAPAMQVADASEVLSMLDADALR
CCCCEEEEEEECCCCCHHHHHHHHHHCEEEEEECCHHHCCHHHHCCHHHHHHHHHHHHHH
AVVAKHHPDYVVPEIEAIRTEVLAEIEADGFNVVPSAYATQMTMNRDAIRDLAAQELGIT
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCHHHHHHHHHHHHCCC
TSRYRYAKNLEEVRAAAEFTGYPCVIKPVMSSSGKGQSTVRSADKLEEAWDYAVANMRGD
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
RKRVIVEQFIDFDYEITLLTVRHKDGITFCPPIGHRQERGDYRESWQPATMSKPAIAAAQ
HHHHHHHHHHCCCEEEEEEEEEECCCCEECCCCCCCHHCCCHHHCCCCCCCCCCHHHHHH
EMAEKVVTALQGNGKGFGLFGVEFFVKGEEVIFSELSPRPHDTGMVTSVSQNLSEFDLHA
HHHHHHHHHHCCCCCEEEEEEEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RAIMGLHVPSEILARPSASAVILAEQESETVSYSGLAAAMEGGADIRIFGKPNTRPYRRM
HHHHCCCCCHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCEEEEEECCCCCHHHHH
GVALATGGDTDYARTAAVAAANKLHIHYGD
CEEEEECCCCCHHHHHHHHHCCEEEEEECC
>Mature Secondary Structure 
PHTAKILLLGSGELGREFVISAKRLGAYVVACDAYAAAPAMQVADASEVLSMLDADALR
CCCEEEEEEECCCCCHHHHHHHHHHCEEEEEECCHHHCCHHHHCCHHHHHHHHHHHHHH
AVVAKHHPDYVVPEIEAIRTEVLAEIEADGFNVVPSAYATQMTMNRDAIRDLAAQELGIT
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCHHHHHHHCCHHHHHHHHHHHHCCC
TSRYRYAKNLEEVRAAAEFTGYPCVIKPVMSSSGKGQSTVRSADKLEEAWDYAVANMRGD
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
RKRVIVEQFIDFDYEITLLTVRHKDGITFCPPIGHRQERGDYRESWQPATMSKPAIAAAQ
HHHHHHHHHHCCCEEEEEEEEEECCCCEECCCCCCCHHCCCHHHCCCCCCCCCCHHHHHH
EMAEKVVTALQGNGKGFGLFGVEFFVKGEEVIFSELSPRPHDTGMVTSVSQNLSEFDLHA
HHHHHHHHHHCCCCCEEEEEEEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RAIMGLHVPSEILARPSASAVILAEQESETVSYSGLAAAMEGGADIRIFGKPNTRPYRRM
HHHHCCCCCHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCEEEEEECCCCCHHHHH
GVALATGGDTDYARTAAVAAANKLHIHYGD
CEEEEECCCCCHHHHHHHHHCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA