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The map label for this gene is mdeA [H]

Identifier: 85374722

GI number: 85374722

Start: 1938111

End: 1939430

Strand: Direct

Name: mdeA [H]

Synonym: ELI_09475

Alternate gene names: 85374722

Gene position: 1938111-1939430 (Clockwise)

Preceding gene: 85374721

Following gene: 85374723

Centisome position: 63.49

GC content: 63.48

Gene sequence:

>1320_bases
ATGACCGACGCCGTCGACGCGACCGACCAGCCGACCCCGCGCCGCAATCCCAAACCCAAGGCGACCACGATCAATGGCCG
TGAGATGAAGCCCAGCACGCTCATGATGGGCCACGGTTTCGATCCAGCGCTTTCGGAGGGCTCGCTCAAAAGCCCGATTT
TCCTCACCAGCACCTTCGCCTTCGAGAACGCCGCGGCAGGCAAGCGCCATTTCGAACGCATCACCGGCCAGCGCCCGGGC
GGTGCCGACGGCCTCGTCTATTCGCGCTTCAACGCGCCCAACCAGGAAATCCTCGAAGACCGGCTGGCGATCTGGGACGG
CGCGGAAGAGGCGCTGAGCTTCTCCAGCGGGATGACCGCCATTGCTATACTGATGCTGGCGGCTTGCAAGGCCGGTGACG
TGATCGTGCATTCCGGCCCGCTCTACGCCGCGTCTGAAGGCTTCGTCGCCAAGACGATGGCGAAATTCGGCGTCACCTAT
GTCGATTTTCCTGCCGGGGCGAGCCGCAAAGAGCTCGACGAAGTGATGCAGAAGGCCAAGGCGCAGGCCGAAGCGCAGGG
CGGCGAAGTGCCGCTGATCTATCTCGAGAGCCCGGGCAATCCGACCAATGCGCTGGTCGATGTCGAAGCCGTACGCGATG
CGCGCGATGCGCATTTCGATGCCGATCGCTGCCCGATTGCGATCGACAACACCTTCCTCGGCCCGCTCTGGCAGCGCCCG
CTCGAACATGGCGCTGACCTGGTGGTCTATTCGCTGACGAAGTATGTCGGCGGTCATTCAGACCTGGTTGCGGGGAGTGT
CGCGGGGAAGAAGCGCTTCATTGATGCAATCCGCGCGCTGCGCAATACGATGGGCGGGATCGCCGATCCCAACACCGCAT
GGATGCTGTGCCGCAGCCTCGAAACCGTGGAACTGCGAATGCAGCGGGCAGGCGAGAACGCAGAAAAGGTCTGCGCATTT
CTCGCCCAGCACCCGAAGGTGGAAGGCCTTGGCTATCTGGGGATGATCAAGGACGATCGGCAGAAGGACATCTACGATCG
GCACTGCCTCGGTGCGGGCAGCACTTTCTCGCTGTTCCTCAAGGGTGGCGAGGCCGAGTGTTTCCGCTTCCTCGACGCGC
TCAAGGTCGCCAAGCTCGCGGTAAGCCTCGGCGGGACGGAGACCCTCGCCAGCCATCCGGCCAGCATGACACACCTCTCG
GTTGCGCATGGCAGGCGGCAGGAGCTCGGCATCACGGACAGCCTCGTGCGGATCAGCATCGGCATCGAAGACGCGGACGA
CCTGATCGCCGATTTCGAACAGGCGCTCGAGGCGGTCTAA

Upstream 100 bases:

>100_bases
CTGCGGAGGTTAGGAACGGGTGCCCCACCGGCCCGACCGGCTCCTGCCTTCCTGGGAGCATGCGGGCACCTCAATGCCCA
GATTGGAAGGACCTGATCCC

Downstream 100 bases:

>100_bases
GTCTTTGCCCCTGTTCTCGTCATTGCGAGGCGACGCAGTCGCCGTGGCAATCCATGGCCAAACTTCTCAGCAAGTACTGT
GTTGCCATGGATCGCCGCGT

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTDAVDATDQPTPRRNPKPKATTINGREMKPSTLMMGHGFDPALSEGSLKSPIFLTSTFAFENAAAGKRHFERITGQRPG
GADGLVYSRFNAPNQEILEDRLAIWDGAEEALSFSSGMTAIAILMLAACKAGDVIVHSGPLYAASEGFVAKTMAKFGVTY
VDFPAGASRKELDEVMQKAKAQAEAQGGEVPLIYLESPGNPTNALVDVEAVRDARDAHFDADRCPIAIDNTFLGPLWQRP
LEHGADLVVYSLTKYVGGHSDLVAGSVAGKKRFIDAIRALRNTMGGIADPNTAWMLCRSLETVELRMQRAGENAEKVCAF
LAQHPKVEGLGYLGMIKDDRQKDIYDRHCLGAGSTFSLFLKGGEAECFRFLDALKVAKLAVSLGGTETLASHPASMTHLS
VAHGRRQELGITDSLVRISIGIEDADDLIADFEQALEAV

Sequences:

>Translated_439_residues
MTDAVDATDQPTPRRNPKPKATTINGREMKPSTLMMGHGFDPALSEGSLKSPIFLTSTFAFENAAAGKRHFERITGQRPG
GADGLVYSRFNAPNQEILEDRLAIWDGAEEALSFSSGMTAIAILMLAACKAGDVIVHSGPLYAASEGFVAKTMAKFGVTY
VDFPAGASRKELDEVMQKAKAQAEAQGGEVPLIYLESPGNPTNALVDVEAVRDARDAHFDADRCPIAIDNTFLGPLWQRP
LEHGADLVVYSLTKYVGGHSDLVAGSVAGKKRFIDAIRALRNTMGGIADPNTAWMLCRSLETVELRMQRAGENAEKVCAF
LAQHPKVEGLGYLGMIKDDRQKDIYDRHCLGAGSTFSLFLKGGEAECFRFLDALKVAKLAVSLGGTETLASHPASMTHLS
VAHGRRQELGITDSLVRISIGIEDADDLIADFEQALEAV
>Mature_438_residues
TDAVDATDQPTPRRNPKPKATTINGREMKPSTLMMGHGFDPALSEGSLKSPIFLTSTFAFENAAAGKRHFERITGQRPGG
ADGLVYSRFNAPNQEILEDRLAIWDGAEEALSFSSGMTAIAILMLAACKAGDVIVHSGPLYAASEGFVAKTMAKFGVTYV
DFPAGASRKELDEVMQKAKAQAEAQGGEVPLIYLESPGNPTNALVDVEAVRDARDAHFDADRCPIAIDNTFLGPLWQRPL
EHGADLVVYSLTKYVGGHSDLVAGSVAGKKRFIDAIRALRNTMGGIADPNTAWMLCRSLETVELRMQRAGENAEKVCAFL
AQHPKVEGLGYLGMIKDDRQKDIYDRHCLGAGSTFSLFLKGGEAECFRFLDALKVAKLAVSLGGTETLASHPASMTHLSV
AHGRRQELGITDSLVRISIGIEDADDLIADFEQALEAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=363, Percent_Identity=35.5371900826446, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI299473759, Length=363, Percent_Identity=33.0578512396694, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI25453487, Length=207, Percent_Identity=39.6135265700483, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1790375, Length=411, Percent_Identity=33.0900243309002, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789383, Length=412, Percent_Identity=28.8834951456311, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=33.8461538461538, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=34.3589743589744, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71980708, Length=395, Percent_Identity=31.6455696202532, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319307, Length=396, Percent_Identity=33.5858585858586, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=27.8801843317972, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=411, Percent_Identity=28.9537712895377, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=304, Percent_Identity=25.9868421052632, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=25, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19922554, Length=362, Percent_Identity=38.6740331491713, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47241; Mature: 47110

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVDATDQPTPRRNPKPKATTINGREMKPSTLMMGHGFDPALSEGSLKSPIFLTSTFA
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEHH
FENAAAGKRHFERITGQRPGGADGLVYSRFNAPNQEILEDRLAIWDGAEEALSFSSGMTA
HCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCHHH
IAILMLAACKAGDVIVHSGPLYAASEGFVAKTMAKFGVTYVDFPAGASRKELDEVMQKAK
HHHHHHHHCCCCCEEEECCCCEECCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHH
AQAEAQGGEVPLIYLESPGNPTNALVDVEAVRDARDAHFDADRCPIAIDNTFLGPLWQRP
HHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHH
LEHGADLVVYSLTKYVGGHSDLVAGSVAGKKRFIDAIRALRNTMGGIADPNTAWMLCRSL
HHCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ETVELRMQRAGENAEKVCAFLAQHPKVEGLGYLGMIKDDRQKDIYDRHCLGAGSTFSLFL
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEE
KGGEAECFRFLDALKVAKLAVSLGGTETLASHPASMTHLSVAHGRRQELGITDSLVRISI
ECCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHEEHHHCCCCHHCCCCCCEEEEEE
GIEDADDLIADFEQALEAV
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDAVDATDQPTPRRNPKPKATTINGREMKPSTLMMGHGFDPALSEGSLKSPIFLTSTFA
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEHH
FENAAAGKRHFERITGQRPGGADGLVYSRFNAPNQEILEDRLAIWDGAEEALSFSSGMTA
HCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCHHH
IAILMLAACKAGDVIVHSGPLYAASEGFVAKTMAKFGVTYVDFPAGASRKELDEVMQKAK
HHHHHHHHCCCCCEEEECCCCEECCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHH
AQAEAQGGEVPLIYLESPGNPTNALVDVEAVRDARDAHFDADRCPIAIDNTFLGPLWQRP
HHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHH
LEHGADLVVYSLTKYVGGHSDLVAGSVAGKKRFIDAIRALRNTMGGIADPNTAWMLCRSL
HHCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ETVELRMQRAGENAEKVCAFLAQHPKVEGLGYLGMIKDDRQKDIYDRHCLGAGSTFSLFL
HHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEE
KGGEAECFRFLDALKVAKLAVSLGGTETLASHPASMTHLSVAHGRRQELGITDSLVRISI
ECCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHEEHHHCCCCHHCCCCCCEEEEEE
GIEDADDLIADFEQALEAV
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]