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The map label for this gene is purN [H]

Identifier: 85374598

GI number: 85374598

Start: 1802903

End: 1803871

Strand: Direct

Name: purN [H]

Synonym: ELI_08855

Alternate gene names: 85374598

Gene position: 1802903-1803871 (Clockwise)

Preceding gene: 85374597

Following gene: 85374604

Centisome position: 59.07

GC content: 64.81

Gene sequence:

>969_bases
TTGGCTGAGACGCCGCTCGCCAAGGTGGCCGTGCTGGTTTCGGGCAGCGGGACCAATATGGCGGCGCTCCTCTATGCCAG
CCGCCTCCCGGACAGCCCCTACGAAATCGTCCTCGTCGCCAGCAACGATCCGAACGCGGGCGGCCTGTCGTTGGCCGAAG
CCGAGGGTATCCCGACCTTTGCGCTCAGCCACAAAGGGATGAGCCGCGAGGAACACGACCAGGCGATGGACGCTGCTGTT
CGAAGTTCCGGCGCAGAATATATCGCGCTCGCCGGATACATGCGCATCCTTTCGGACGAGATGGTCACGCGGTGGGAAGG
CCGGATGCTCAACATCCACCCGTCGCTTCTGCCCAAATACAAGGGATTGAAAACCCACGAACGCGCGCTCGAGGCGGGCG
ACGAATTTTGCGGCACGTCGGTGCATCTCGTCACCAGCGAGCTCGACGGTGGCCAGGTGCTGGGGCAAGCTCCCGTTGCG
ATCATGGACAGCGACACGCCCGAAACGCTCGCCTACCGGGTCAAGCTGGCCGAGCACCAGCTTTACCCGCGCGTGCTGGC
CGACTTCGTCTCGCGCCCGTTCGATTCCGGCTGGCTTCTGGAACGGGTGCGCGCGCTGGCGATGGCGCTTCCCGAAGCCG
AGGAGCGCCCCAGCCACGGCGCGCCCGGCTGGCGCACCGGCGGCATGAGCGGGAAGTATTTCGCCTATTTCAGCGACCGG
CACCACGGCGAGGATAATATCGCCCTGCTCGCCAAGACCAGCGGCACCGACGAGCTGATGGAACTCGTCGAGCGCGATCC
GGACGCATTCTACAGACCCGCCTACTATGGCGCGAGCGGCTGGGTCGGCGTGATCCTCAACCGCCCGAATTGCGATTGGG
ATCAGGTCGAGTACTGGCTGAAGCGCAGCTGGCAGGCGGTCGCACCGAAGCGCCTGACCAAGATGATGGATGTGGCTGAC
GAGTGTTAG

Upstream 100 bases:

>100_bases
GTCCACCGCATCGGAACGATCGAGGCGGGTGAGAAAGGCTGCACCGTGCGCGGCTCGACAGGCACATGGTCCGCCACCGC
CGATTGGGAAACCCGGCATC

Downstream 100 bases:

>100_bases
GTCCGCTTGCGACCCAATTGCGGATATGCAGATGTTCGGGCATCTACCTGCAAATGAGCCAACCGCCCTCCAAGTCTGAG
CGCATGGAAGTCCGCAAAGC

Product: phosphoribosylglycinamide formyltransferase protein

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MAETPLAKVAVLVSGSGTNMAALLYASRLPDSPYEIVLVASNDPNAGGLSLAEAEGIPTFALSHKGMSREEHDQAMDAAV
RSSGAEYIALAGYMRILSDEMVTRWEGRMLNIHPSLLPKYKGLKTHERALEAGDEFCGTSVHLVTSELDGGQVLGQAPVA
IMDSDTPETLAYRVKLAEHQLYPRVLADFVSRPFDSGWLLERVRALAMALPEAEERPSHGAPGWRTGGMSGKYFAYFSDR
HHGEDNIALLAKTSGTDELMELVERDPDAFYRPAYYGASGWVGVILNRPNCDWDQVEYWLKRSWQAVAPKRLTKMMDVAD
EC

Sequences:

>Translated_322_residues
MAETPLAKVAVLVSGSGTNMAALLYASRLPDSPYEIVLVASNDPNAGGLSLAEAEGIPTFALSHKGMSREEHDQAMDAAV
RSSGAEYIALAGYMRILSDEMVTRWEGRMLNIHPSLLPKYKGLKTHERALEAGDEFCGTSVHLVTSELDGGQVLGQAPVA
IMDSDTPETLAYRVKLAEHQLYPRVLADFVSRPFDSGWLLERVRALAMALPEAEERPSHGAPGWRTGGMSGKYFAYFSDR
HHGEDNIALLAKTSGTDELMELVERDPDAFYRPAYYGASGWVGVILNRPNCDWDQVEYWLKRSWQAVAPKRLTKMMDVAD
EC
>Mature_321_residues
AETPLAKVAVLVSGSGTNMAALLYASRLPDSPYEIVLVASNDPNAGGLSLAEAEGIPTFALSHKGMSREEHDQAMDAAVR
SSGAEYIALAGYMRILSDEMVTRWEGRMLNIHPSLLPKYKGLKTHERALEAGDEFCGTSVHLVTSELDGGQVLGQAPVAI
MDSDTPETLAYRVKLAEHQLYPRVLADFVSRPFDSGWLLERVRALAMALPEAEERPSHGAPGWRTGGMSGKYFAYFSDRH
HGEDNIALLAKTSGTDELMELVERDPDAFYRPAYYGASGWVGVILNRPNCDWDQVEYWLKRSWQAVAPKRLTKMMDVADE
C

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=45.945945945946, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=45.945945945946, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=45.945945945946, Blast_Score=168, Evalue=5e-42,
Organism=Escherichia coli, GI1788846, Length=183, Percent_Identity=43.7158469945355, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787483, Length=179, Percent_Identity=30.7262569832402, Blast_Score=94, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=40.2234636871508, Blast_Score=151, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=43.3862433862434, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 35437; Mature: 35306

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETPLAKVAVLVSGSGTNMAALLYASRLPDSPYEIVLVASNDPNAGGLSLAEAEGIPTF
CCCCCCEEEEEEEECCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCCCCEEHHCCCCCEE
ALSHKGMSREEHDQAMDAAVRSSGAEYIALAGYMRILSDEMVTRWEGRMLNIHPSLLPKY
EECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCC
KGLKTHERALEAGDEFCGTSVHLVTSELDGGQVLGQAPVAIMDSDTPETLAYRVKLAEHQ
CCCHHHHHHHHCCHHHCCCEEEEEEECCCCCEEECCCCEEEECCCCCHHHHHHHHHHHHH
LYPRVLADFVSRPFDSGWLLERVRALAMALPEAEERPSHGAPGWRTGGMSGKYFAYFSDR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEEEECCC
HHGEDNIALLAKTSGTDELMELVERDPDAFYRPAYYGASGWVGVILNRPNCDWDQVEYWL
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEECCCCCEEEEEECCCCCHHHHHHHH
KRSWQAVAPKRLTKMMDVADEC
HHCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AETPLAKVAVLVSGSGTNMAALLYASRLPDSPYEIVLVASNDPNAGGLSLAEAEGIPTF
CCCCCEEEEEEEECCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCCCCEEHHCCCCCEE
ALSHKGMSREEHDQAMDAAVRSSGAEYIALAGYMRILSDEMVTRWEGRMLNIHPSLLPKY
EECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCC
KGLKTHERALEAGDEFCGTSVHLVTSELDGGQVLGQAPVAIMDSDTPETLAYRVKLAEHQ
CCCHHHHHHHHCCHHHCCCEEEEEEECCCCCEEECCCCEEEECCCCCHHHHHHHHHHHHH
LYPRVLADFVSRPFDSGWLLERVRALAMALPEAEERPSHGAPGWRTGGMSGKYFAYFSDR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCEEEEECCC
HHGEDNIALLAKTSGTDELMELVERDPDAFYRPAYYGASGWVGVILNRPNCDWDQVEYWL
CCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCEECCCCCEEEEEECCCCCHHHHHHHH
KRSWQAVAPKRLTKMMDVADEC
HHCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]