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The map label for this gene is lptD [H]

Identifier: 85374604

GI number: 85374604

Start: 1807695

End: 1809926

Strand: Direct

Name: lptD [H]

Synonym: ELI_08885

Alternate gene names: 85374604

Gene position: 1807695-1809926 (Clockwise)

Preceding gene: 85374598

Following gene: 85374605

Centisome position: 59.22

GC content: 64.47

Gene sequence:

>2232_bases
TTGCCCGACCTGCTGCTCCGCGCATCGCGTGCCGGGGCAGGCGCGCTGGCTTTGGCGGGCCTGTCGGTTCCGACGCTGGC
GCAAGACGAGACGGTCGACGCGGCCAGCACAGAGCAGCGGCAGGAACCGCGCGCGATCGATTTCGAGGCCGACGTCGTCG
ATTACGATTCCGGGCAGGACGTTGTTTCCGCACGCGGCAATGTGGTCCTGCGCAGCGAGGATCAGTCGGTCCGCGCCGAT
GTCGTGCGGTGGGATCGCTCGACCGGCGTCATCGAAGCGATCGGAAATGTCCGCTTCGTCGATCAGGACGGCAACCAGCT
TTATTCCAGCAGCGTCGAGCTGACCGACAAGTTCGAAGCAGGGGCAATGGAAGACCTGCTGCTGGCGCTGCGCGAGGGCG
GGCGACTGGCGGCAGAATCAGGCGCGCGCGGCGAAGACGGCACCATTGTGCTCGATCGCGCCGCCTATACCGCCTGCTCG
GTCACCAATCCCGACGGCTGCCCCAAGGACCCCAGCTGGCGAATCACTGCCGAGCGCGTCGTTTACGATCCGGACGAACG
CCGGGTGCGCTTCCGCGGGGCCTATCTCGAATTGTTCGGCGCGCGCCTGCTGCCGCTGCCGGGTCTGAGCGTGCGCACCG
ATGGCGGCGCGATCAGCGGATTCCTCGTGCCCGACATCAGGCTGTCGGAATCGAACGGCCTGGAAATGACCGGCGAATAC
TATTTCAGGCTCGGCGACAACAAGGACGCCAAGCTGGGTACGAGCGTCTATACCGAAGCGCCGCCGATGGTGTTCGGCCA
GTATCGCCAGCTTACCGAAAAAGGCGCCTTCCAGATAACGGGCTACGGCACCTACAGCCGCCGTATCTCCGATTTCACCG
GCATCGCGACGAGCGAGCAGGACTTCCGCGGGTATGTCGATGCCAATGGCACGTTTCAGTTCGACGAGAACTGGAGCCTC
AACGCTTCGCTGCGGCTGGCCAGCGATCGGACCTTCCTGCGCCGCTACGATATCAGCCGCGACGACCGCCTACGCTCGAT
CGTCGAGCTCGAACGCATCGAAAGCGACAGCTATTTCCAGCTCGCGGGCTACGCCACGCAGACCCTGCGACTCAACCAGG
ACCAGGGGCAGGTACCGATGGCCTTCCCCGTACTCGATTACCGCAAGCGGCTGGACGAGCCCGTGCTCGGGGGCAAGGTG
GAGATGCAGCTCAACACGCTGGCGATCGTGCGCGATGTGGGTCAGGATACCCAGCGGGCCTTCGCCAAGGTGCAGTGGGA
CCTGCGCAAGATCACTCCGATGGGGCAGGTCGTCCGGTTCACCGCGCTCGCTCGCGGCGATGTCTATCATTCAAGCCAGA
ATGCGCTGACCCAAACCGCGATCTATCGCGGCAATCCCGGCTGGCAGGCGCGAGGTATCGGTCTGGCGGCAGTCGATGTC
CAATGGCCCTTTGTCGGGCCTGCGTTCGGCGGAACGCAGGTTTTCACGCCGCGCGTGCAGGTGGTCGCGACGCCGCCGAT
CCGCAATCTGGCGGTGCCTAACGAGGATGCCCGCGCGATCGATCTCGAGGATTCCAACCTCTTCGCGCTGAACCGCTTCC
CGGGTTACGATCGGGTCGAGGACGGCGTACGCATCACCTACGGTTTCGACTGGGAGCTGACCCGGCCCGGCCTGAGGGTG
AAGACGACGCTCGGCCAGAGCTACCGGCTCGACAAGGATACCGATATCCTCGTCGACGGGACCGGCCTGTCGGAACGCGT
GTCCGACTTCGTCGGCCGCACCGAGGTGCGCTTTCGCGACTTCGTCTCGCTCACGCACCGCTACCGCCTCGACAAGGACA
ATCTGGCGATCCGCCGCAACGAATTCGATGCGACCATCGGCAGCCGCAGGACGTATTTCGAAGTCGGCTACCTGCGCCTC
GACCGCGATATTGCGCAAGGCATCGAGGATCTGCAGGATCGCGAAGAGGTGCGCGCCGCTGCCCGCCTTGCCTTTGCCGA
TTACTGGTCGATCTTCGGAGCCGGCGTCGTCAACCTGACCGATCGCAACGAGGACCCGACCTTCATCTCCGATGGCTTCG
AGCCGATCCGCACCCGGCTCGGCATCGCTTACGAGGACGATTGCCTGGAACTCGGCTTCACCTGGCGGCGCGATTATGTG
GACCAGGGGGACGCCCGGCGCGGCGACACGTTCCAGTTCTATTTCAGCCTGAAAAGTCTCGGATTCCGATAG

Upstream 100 bases:

>100_bases
AGGCGTTTCCACGCTCTGCCGCATGCGGCGTCACAGGGCGATTGCGATTAGGATGCCCAAGTGCAATAGGCAAGCCATGC
ACCGCCCCGGAGCTCCTGCC

Downstream 100 bases:

>100_bases
CGACACCGCCGTGGAGCAATGCCGGATTGGCTTGACCAGCCAAACGCGCTAGATCGCGCCACTCATCAAGGGTTCAGCCA
CGTTTCGCCATGCGGCACGG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MPDLLLRASRAGAGALALAGLSVPTLAQDETVDAASTEQRQEPRAIDFEADVVDYDSGQDVVSARGNVVLRSEDQSVRAD
VVRWDRSTGVIEAIGNVRFVDQDGNQLYSSSVELTDKFEAGAMEDLLLALREGGRLAAESGARGEDGTIVLDRAAYTACS
VTNPDGCPKDPSWRITAERVVYDPDERRVRFRGAYLELFGARLLPLPGLSVRTDGGAISGFLVPDIRLSESNGLEMTGEY
YFRLGDNKDAKLGTSVYTEAPPMVFGQYRQLTEKGAFQITGYGTYSRRISDFTGIATSEQDFRGYVDANGTFQFDENWSL
NASLRLASDRTFLRRYDISRDDRLRSIVELERIESDSYFQLAGYATQTLRLNQDQGQVPMAFPVLDYRKRLDEPVLGGKV
EMQLNTLAIVRDVGQDTQRAFAKVQWDLRKITPMGQVVRFTALARGDVYHSSQNALTQTAIYRGNPGWQARGIGLAAVDV
QWPFVGPAFGGTQVFTPRVQVVATPPIRNLAVPNEDARAIDLEDSNLFALNRFPGYDRVEDGVRITYGFDWELTRPGLRV
KTTLGQSYRLDKDTDILVDGTGLSERVSDFVGRTEVRFRDFVSLTHRYRLDKDNLAIRRNEFDATIGSRRTYFEVGYLRL
DRDIAQGIEDLQDREEVRAAARLAFADYWSIFGAGVVNLTDRNEDPTFISDGFEPIRTRLGIAYEDDCLELGFTWRRDYV
DQGDARRGDTFQFYFSLKSLGFR

Sequences:

>Translated_743_residues
MPDLLLRASRAGAGALALAGLSVPTLAQDETVDAASTEQRQEPRAIDFEADVVDYDSGQDVVSARGNVVLRSEDQSVRAD
VVRWDRSTGVIEAIGNVRFVDQDGNQLYSSSVELTDKFEAGAMEDLLLALREGGRLAAESGARGEDGTIVLDRAAYTACS
VTNPDGCPKDPSWRITAERVVYDPDERRVRFRGAYLELFGARLLPLPGLSVRTDGGAISGFLVPDIRLSESNGLEMTGEY
YFRLGDNKDAKLGTSVYTEAPPMVFGQYRQLTEKGAFQITGYGTYSRRISDFTGIATSEQDFRGYVDANGTFQFDENWSL
NASLRLASDRTFLRRYDISRDDRLRSIVELERIESDSYFQLAGYATQTLRLNQDQGQVPMAFPVLDYRKRLDEPVLGGKV
EMQLNTLAIVRDVGQDTQRAFAKVQWDLRKITPMGQVVRFTALARGDVYHSSQNALTQTAIYRGNPGWQARGIGLAAVDV
QWPFVGPAFGGTQVFTPRVQVVATPPIRNLAVPNEDARAIDLEDSNLFALNRFPGYDRVEDGVRITYGFDWELTRPGLRV
KTTLGQSYRLDKDTDILVDGTGLSERVSDFVGRTEVRFRDFVSLTHRYRLDKDNLAIRRNEFDATIGSRRTYFEVGYLRL
DRDIAQGIEDLQDREEVRAAARLAFADYWSIFGAGVVNLTDRNEDPTFISDGFEPIRTRLGIAYEDDCLELGFTWRRDYV
DQGDARRGDTFQFYFSLKSLGFR
>Mature_742_residues
PDLLLRASRAGAGALALAGLSVPTLAQDETVDAASTEQRQEPRAIDFEADVVDYDSGQDVVSARGNVVLRSEDQSVRADV
VRWDRSTGVIEAIGNVRFVDQDGNQLYSSSVELTDKFEAGAMEDLLLALREGGRLAAESGARGEDGTIVLDRAAYTACSV
TNPDGCPKDPSWRITAERVVYDPDERRVRFRGAYLELFGARLLPLPGLSVRTDGGAISGFLVPDIRLSESNGLEMTGEYY
FRLGDNKDAKLGTSVYTEAPPMVFGQYRQLTEKGAFQITGYGTYSRRISDFTGIATSEQDFRGYVDANGTFQFDENWSLN
ASLRLASDRTFLRRYDISRDDRLRSIVELERIESDSYFQLAGYATQTLRLNQDQGQVPMAFPVLDYRKRLDEPVLGGKVE
MQLNTLAIVRDVGQDTQRAFAKVQWDLRKITPMGQVVRFTALARGDVYHSSQNALTQTAIYRGNPGWQARGIGLAAVDVQ
WPFVGPAFGGTQVFTPRVQVVATPPIRNLAVPNEDARAIDLEDSNLFALNRFPGYDRVEDGVRITYGFDWELTRPGLRVK
TTLGQSYRLDKDTDILVDGTGLSERVSDFVGRTEVRFRDFVSLTHRYRLDKDNLAIRRNEFDATIGSRRTYFEVGYLRLD
RDIAQGIEDLQDREEVRAAARLAFADYWSIFGAGVVNLTDRNEDPTFISDGFEPIRTRLGIAYEDDCLELGFTWRRDYVD
QGDARRGDTFQFYFSLKSLGFR

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 83267; Mature: 83136

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLRASRAGAGALALAGLSVPTLAQDETVDAASTEQRQEPRAIDFEADVVDYDSGQD
CCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCEECEECCEEECCCCCH
VVSARGNVVLRSEDQSVRADVVRWDRSTGVIEAIGNVRFVDQDGNQLYSSSVELTDKFEA
HHHCCCCEEEECCCCCHHHHHEEECCCCCHHEECCCEEEEECCCCEEECCCCCEEECCCC
GAMEDLLLALREGGRLAAESGARGEDGTIVLDRAAYTACSVTNPDGCPKDPSWRITAERV
CCHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCEEEEEEEE
VYDPDERRVRFRGAYLELFGARLLPLPGLSVRTDGGAISGFLVPDIRLSESNGLEMTGEY
EECCCCCEEEEEHHHHHHHCCCCCCCCCCEEECCCCEEEEEEECCEEECCCCCCEEECEE
YFRLGDNKDAKLGTSVYTEAPPMVFGQYRQLTEKGAFQITGYGTYSRRISDFTGIATSEQ
EEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCH
DFRGYVDANGTFQFDENWSLNASLRLASDRTFLRRYDISRDDRLRSIVELERIESDSYFQ
HCCEEECCCCEEEECCCCCCCEEEEEECCCEEHHHCCCCHHHHHHHHHHHHHCCCCCEEE
LAGYATQTLRLNQDQGQVPMAFPVLDYRKRLDEPVLGGKVEMQLNTLAIVRDVGQDTQRA
EECCEEEEEEECCCCCCCCEEECHHHHHHHCCCCCCCCEEEEEEHHEEEHHHCCCHHHHH
FAKVQWDLRKITPMGQVVRFTALARGDVYHSSQNALTQTAIYRGNPGWQARGIGLAAVDV
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCCCCCEEEEEEE
QWPFVGPAFGGTQVFTPRVQVVATPPIRNLAVPNEDARAIDLEDSNLFALNRFPGYDRVE
ECCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCC
DGVRITYGFDWELTRPGLRVKTTLGQSYRLDKDTDILVDGTGLSERVSDFVGRTEVRFRD
CCEEEEEECCEEECCCCEEEEEECCCCEECCCCCEEEEECCCHHHHHHHHHCCCHHHHHH
FVSLTHRYRLDKDNLAIRRNEFDATIGSRRTYFEVGYLRLDRDIAQGIEDLQDREEVRAA
HHHHHHHEECCCCCEEEEECCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHH
ARLAFADYWSIFGAGVVNLTDRNEDPTFISDGFEPIRTRLGIAYEDDCLELGFTWRRDYV
HHHHHHHHHHHHCCCEEEEECCCCCCCEECCCCHHHHHHCCCEECCCHHCCCCEECCCCC
DQGDARRGDTFQFYFSLKSLGFR
CCCCCCCCCEEEEEEEEHHCCCC
>Mature Secondary Structure 
PDLLLRASRAGAGALALAGLSVPTLAQDETVDAASTEQRQEPRAIDFEADVVDYDSGQD
CCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCCCEECEECCEEECCCCCH
VVSARGNVVLRSEDQSVRADVVRWDRSTGVIEAIGNVRFVDQDGNQLYSSSVELTDKFEA
HHHCCCCEEEECCCCCHHHHHEEECCCCCHHEECCCEEEEECCCCEEECCCCCEEECCCC
GAMEDLLLALREGGRLAAESGARGEDGTIVLDRAAYTACSVTNPDGCPKDPSWRITAERV
CCHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCEEEEEEEE
VYDPDERRVRFRGAYLELFGARLLPLPGLSVRTDGGAISGFLVPDIRLSESNGLEMTGEY
EECCCCCEEEEEHHHHHHHCCCCCCCCCCEEECCCCEEEEEEECCEEECCCCCCEEECEE
YFRLGDNKDAKLGTSVYTEAPPMVFGQYRQLTEKGAFQITGYGTYSRRISDFTGIATSEQ
EEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCH
DFRGYVDANGTFQFDENWSLNASLRLASDRTFLRRYDISRDDRLRSIVELERIESDSYFQ
HCCEEECCCCEEEECCCCCCCEEEEEECCCEEHHHCCCCHHHHHHHHHHHHHCCCCCEEE
LAGYATQTLRLNQDQGQVPMAFPVLDYRKRLDEPVLGGKVEMQLNTLAIVRDVGQDTQRA
EECCEEEEEEECCCCCCCCEEECHHHHHHHCCCCCCCCEEEEEEHHEEEHHHCCCHHHHH
FAKVQWDLRKITPMGQVVRFTALARGDVYHSSQNALTQTAIYRGNPGWQARGIGLAAVDV
HHHHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCCCCCCEEEEEEE
QWPFVGPAFGGTQVFTPRVQVVATPPIRNLAVPNEDARAIDLEDSNLFALNRFPGYDRVE
ECCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCC
DGVRITYGFDWELTRPGLRVKTTLGQSYRLDKDTDILVDGTGLSERVSDFVGRTEVRFRD
CCEEEEEECCEEECCCCEEEEEECCCCEECCCCCEEEEECCCHHHHHHHHHCCCHHHHHH
FVSLTHRYRLDKDNLAIRRNEFDATIGSRRTYFEVGYLRLDRDIAQGIEDLQDREEVRAA
HHHHHHHEECCCCCEEEEECCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHH
ARLAFADYWSIFGAGVVNLTDRNEDPTFISDGFEPIRTRLGIAYEDDCLELGFTWRRDYV
HHHHHHHHHHHHCCCEEEEECCCCCCCEECCCCHHHHHHCCCEECCCHHCCCCEECCCCC
DQGDARRGDTFQFYFSLKSLGFR
CCCCCCCCCEEEEEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA