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The map label for this gene is greB [H]

Identifier: 85374381

GI number: 85374381

Start: 1610557

End: 1611036

Strand: Direct

Name: greB [H]

Synonym: ELI_07770

Alternate gene names: 85374381

Gene position: 1610557-1611036 (Clockwise)

Preceding gene: 85374380

Following gene: 85374382

Centisome position: 52.76

GC content: 64.38

Gene sequence:

>480_bases
ATGTCGCAGCCGAGCCATCCCATCACGCCCGGCGGCTATGCTGCGCTGAAGGCACGCTACGACCGTTTGCTCGGCACCGA
GCGGCCCGAGATTGTCGAGATCGTCAGCTGGGCGGCGGGCAATGGCGATCGCAGCGAGAATGGCGACTATATCTATGGTC
GTAAAAGAATGCGCGAGATCGACCGCGAGCTTGCCCATCTCGCGCGGCGCATGAAGCATGCCCGCGTAATCGAGCCCTCC
GAACAGCCCGACAAGGACCGCGCCTTCTTCGGTGCCACGCTGACGCTGGCGGACGAAGACGATGCCCGGAAAACCGTCAC
GCTGGTCGGCGACGACGAGCAGGATGCCAGCGCCGGAAAGATCGGCTGGTCGAGCCCGCTCGCCCGCGCACTCAGGGGTG
CGCGTGTCGGGGATCTTCGCACCGTGCACCTTCCTTCGGGCGAGAGAGAATGGGAAGTGATGGCGATCGCCTATGCATAA

Upstream 100 bases:

>100_bases
CGTGATCGAGAATGCCGCCGTGTACGAGCATCTCTATCCCGACAACGCCCCCATGGGCCGCCCGCGCATGGCACGGGACT
TCCTGCGGTAGTCACTACTC

Downstream 100 bases:

>100_bases
GCTGTTGCCCTTTGTCGCCCTGCTCGCCTTCGCCGAACCTGTCGCAGCCAAGGATTCGCTCGGCGTGTTCGGCGAATGGG
GTGCATTTCGCGATCCGCAG

Product: transcription elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSQPSHPITPGGYAALKARYDRLLGTERPEIVEIVSWAAGNGDRSENGDYIYGRKRMREIDRELAHLARRMKHARVIEPS
EQPDKDRAFFGATLTLADEDDARKTVTLVGDDEQDASAGKIGWSSPLARALRGARVGDLRTVHLPSGEREWEVMAIAYA

Sequences:

>Translated_159_residues
MSQPSHPITPGGYAALKARYDRLLGTERPEIVEIVSWAAGNGDRSENGDYIYGRKRMREIDRELAHLARRMKHARVIEPS
EQPDKDRAFFGATLTLADEDDARKTVTLVGDDEQDASAGKIGWSSPLARALRGARVGDLRTVHLPSGEREWEVMAIAYA
>Mature_158_residues
SQPSHPITPGGYAALKARYDRLLGTERPEIVEIVSWAAGNGDRSENGDYIYGRKRMREIDRELAHLARRMKHARVIEPSE
QPDKDRAFFGATLTLADEDDARKTVTLVGDDEQDASAGKIGWSSPLARALRGARVGDLRTVHLPSGEREWEVMAIAYA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=40.7894736842105, Blast_Score=116, Evalue=5e-28,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17673; Mature: 17542

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPSHPITPGGYAALKARYDRLLGTERPEIVEIVSWAAGNGDRSENGDYIYGRKRMREI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHH
DRELAHLARRMKHARVIEPSEQPDKDRAFFGATLTLADEDDARKTVTLVGDDEQDASAGK
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
IGWSSPLARALRGARVGDLRTVHLPSGEREWEVMAIAYA
CCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure 
SQPSHPITPGGYAALKARYDRLLGTERPEIVEIVSWAAGNGDRSENGDYIYGRKRMREI
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHH
DRELAHLARRMKHARVIEPSEQPDKDRAFFGATLTLADEDDARKTVTLVGDDEQDASAGK
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
IGWSSPLARALRGARVGDLRTVHLPSGEREWEVMAIAYA
CCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]