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The map label for this gene is yjbJ [H]

Identifier: 85374380

GI number: 85374380

Start: 1608670

End: 1610547

Strand: Direct

Name: yjbJ [H]

Synonym: ELI_07765

Alternate gene names: 85374380

Gene position: 1608670-1610547 (Clockwise)

Preceding gene: 85374377

Following gene: 85374381

Centisome position: 52.7

GC content: 66.61

Gene sequence:

>1878_bases
ATGGCGCAGGACGCCGCCAGCTGGGACCGTGCGCGCTCCAGTCTCGTGGCCGCGCAGCCGAGCCGGATGGCGCAGGTGAT
CGACCGCTGGGATTATCTCATAGGGCAGGATAATCTCTCGTTCGACCAATATGCCGGTTTCGTCCTCGCCTATCCCGACT
TTCCACAGGCCGACCGGCTGCGCGTCCGTGCCGAGGAAGCGCTCGACGATGAGCCGATCGGACCCGAACGGCTGGTTGCT
TATTTCGACCAGAACCCGCCGCTGACCAATACCGCGCGCGGGCGCTATGCGCTGGCGCTGGCCAATGTCGGTCGCCCCGA
AGCCGCCGAGGAAGCGCGCAAGGCGTGGCGCGGCGGCACCATGAGCGGGCCGTCAGAAGCCTACCTGATGGGGCTATACG
GCAGCCGCTTCACCCAGGCCGATCACGATGCGCGGATGAACGCGCTACTGTGGCAACGCAACGCCGAGGCCGCCGTGCGC
CAGATCAACAACGTCTCGCCTGCAGAGCGCGCCGGCTTCATGGCACGGCTCTCGCTGATCCAGGATACGGCGCCCTCCAG
CATCGGCCTGTCCGCACCCTCCGGCGCACGCAGCGATGCTGGCTATGTCTACAACCTTGCGCGTTATTACCGCACCAACC
GCAATCTTCCCGCGGCGATCGACTTGCTCGTCAATCGCCCGCAGTTCGATGCGCCCGCGCTCGATCCTGAGGTGATGATC
GGCGAAATGCTGCGCATCGCGCAAGGTGCCGGGTCCGATCCCGCCATTCGCATCGCCTCCAAGGTGGACGACATTTTTCC
GGCCGGGGCCGATATCAGCGGCATGTCCTACCGCCTGCGCGACGATTACACTTCGCTGATGTGGCTCGGCGGGACCAAGG
CGCTGTGGAACCTCGCCGACGGTTCGCGCGCCGCGCCCCTCTTCTATCGCTACGGTGCCGCCGCACGCACGCCGCCGACG
CGGTCGAAAGGTTTCTACTGGGCAGGCCTCGCCGCGGAGCAGGCCGGGCAAACGTCGGAGGCTCGACGCTATTTCGAAAT
GGCGGCGGGCTACCCCGATCGCTATTATGGCCAGCTCGCGCTCGGCAAGCTCGGCCGCGACCCTCAACTGGCAAACAAGG
CCGTGCCCGAGCCGAGTTTCGAACAGCGCGCCGCGTTCGAGAACGCCTCGCTGACGCGCGCCGTCAGGGAGGTCGCCCGC
GGCGCGCCCTGGCGCACCGGCATCCGCTTCTACCAGGAAATCGCCCAGAGCGCCGATACGCCCGAGGAACACGCGCTGCG
CGCCGAACTGGCCCGCGAGATCGGCCGGCGGGACCTGGCCGTCAATGTCGCCGAGGCCGCCGGTGCCGACGGGCTCAACC
AGTTCGTAGCGCAGGGTTTCCCGCGCTTGCAGACGCCGCCGGGCGTCGACTGGACCATGGTCCACGCGATCAGCCGGCAG
GAAAGCCAGTTCGCCGAAAACGCCATCAGCCACGCCGGTGCGCGCGGGTTGATGCAGCTGATGCCGGGCACTGCGCGCGA
ACAGGCGGGCAAGCTCGGCATGAGCTACATGCAGGCCAACCTGATCGAAAGCCCCAGCTACAACATCCAGCTCGGCAATG
GCTATTTCGCGCGGATGATGGACTATTACGGCGGGGCCTACCCGCTCGCCATCGCCGCCTACAACGCCGGGCCGGGCAAC
GTGAACAAATGGCTGCGCTCCAATGGCGACCCGCGCCGGGGCGATATCGATTACGTGACGTGGATCGAGCGGATCCCGAT
CTTCGAGACCAAGAATTACGTCACACGCGTGATCGAGAATGCCGCCGTGTACGAGCATCTCTATCCCGACAACGCCCCCA
TGGGCCGCCCGCGCATGGCACGGGACTTCCTGCGGTAG

Upstream 100 bases:

>100_bases
AGTGCCTGATAAGGAGAGCTTGGCCAGAATGTCCAGCATGACACGTATTCCCTTTTTAGCCGTAGCGCTTCTCGCAACCA
CTTCGCTCAACCCCCCGGCC

Downstream 100 bases:

>100_bases
TCACTACTCATGTCGCAGCCGAGCCATCCCATCACGCCCGGCGGCTATGCTGCGCTGAAGGCACGCTACGACCGTTTGCT
CGGCACCGAGCGGCCCGAGA

Product: transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MAQDAASWDRARSSLVAAQPSRMAQVIDRWDYLIGQDNLSFDQYAGFVLAYPDFPQADRLRVRAEEALDDEPIGPERLVA
YFDQNPPLTNTARGRYALALANVGRPEAAEEARKAWRGGTMSGPSEAYLMGLYGSRFTQADHDARMNALLWQRNAEAAVR
QINNVSPAERAGFMARLSLIQDTAPSSIGLSAPSGARSDAGYVYNLARYYRTNRNLPAAIDLLVNRPQFDAPALDPEVMI
GEMLRIAQGAGSDPAIRIASKVDDIFPAGADISGMSYRLRDDYTSLMWLGGTKALWNLADGSRAAPLFYRYGAAARTPPT
RSKGFYWAGLAAEQAGQTSEARRYFEMAAGYPDRYYGQLALGKLGRDPQLANKAVPEPSFEQRAAFENASLTRAVREVAR
GAPWRTGIRFYQEIAQSADTPEEHALRAELAREIGRRDLAVNVAEAAGADGLNQFVAQGFPRLQTPPGVDWTMVHAISRQ
ESQFAENAISHAGARGLMQLMPGTAREQAGKLGMSYMQANLIESPSYNIQLGNGYFARMMDYYGGAYPLAIAAYNAGPGN
VNKWLRSNGDPRRGDIDYVTWIERIPIFETKNYVTRVIENAAVYEHLYPDNAPMGRPRMARDFLR

Sequences:

>Translated_625_residues
MAQDAASWDRARSSLVAAQPSRMAQVIDRWDYLIGQDNLSFDQYAGFVLAYPDFPQADRLRVRAEEALDDEPIGPERLVA
YFDQNPPLTNTARGRYALALANVGRPEAAEEARKAWRGGTMSGPSEAYLMGLYGSRFTQADHDARMNALLWQRNAEAAVR
QINNVSPAERAGFMARLSLIQDTAPSSIGLSAPSGARSDAGYVYNLARYYRTNRNLPAAIDLLVNRPQFDAPALDPEVMI
GEMLRIAQGAGSDPAIRIASKVDDIFPAGADISGMSYRLRDDYTSLMWLGGTKALWNLADGSRAAPLFYRYGAAARTPPT
RSKGFYWAGLAAEQAGQTSEARRYFEMAAGYPDRYYGQLALGKLGRDPQLANKAVPEPSFEQRAAFENASLTRAVREVAR
GAPWRTGIRFYQEIAQSADTPEEHALRAELAREIGRRDLAVNVAEAAGADGLNQFVAQGFPRLQTPPGVDWTMVHAISRQ
ESQFAENAISHAGARGLMQLMPGTAREQAGKLGMSYMQANLIESPSYNIQLGNGYFARMMDYYGGAYPLAIAAYNAGPGN
VNKWLRSNGDPRRGDIDYVTWIERIPIFETKNYVTRVIENAAVYEHLYPDNAPMGRPRMARDFLR
>Mature_624_residues
AQDAASWDRARSSLVAAQPSRMAQVIDRWDYLIGQDNLSFDQYAGFVLAYPDFPQADRLRVRAEEALDDEPIGPERLVAY
FDQNPPLTNTARGRYALALANVGRPEAAEEARKAWRGGTMSGPSEAYLMGLYGSRFTQADHDARMNALLWQRNAEAAVRQ
INNVSPAERAGFMARLSLIQDTAPSSIGLSAPSGARSDAGYVYNLARYYRTNRNLPAAIDLLVNRPQFDAPALDPEVMIG
EMLRIAQGAGSDPAIRIASKVDDIFPAGADISGMSYRLRDDYTSLMWLGGTKALWNLADGSRAAPLFYRYGAAARTPPTR
SKGFYWAGLAAEQAGQTSEARRYFEMAAGYPDRYYGQLALGKLGRDPQLANKAVPEPSFEQRAAFENASLTRAVREVARG
APWRTGIRFYQEIAQSADTPEEHALRAELAREIGRRDLAVNVAEAAGADGLNQFVAQGFPRLQTPPGVDWTMVHAISRQE
SQFAENAISHAGARGLMQLMPGTAREQAGKLGMSYMQANLIESPSYNIQLGNGYFARMMDYYGGAYPLAIAAYNAGPGNV
NKWLRSNGDPRRGDIDYVTWIERIPIFETKNYVTRVIENAAVYEHLYPDNAPMGRPRMARDFLR

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=153, Percent_Identity=34.640522875817, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 69020; Mature: 68889

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDAASWDRARSSLVAAQPSRMAQVIDRWDYLIGQDNLSFDQYAGFVLAYPDFPQADRL
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCCCHHH
RVRAEEALDDEPIGPERLVAYFDQNPPLTNTARGRYALALANVGRPEAAEEARKAWRGGT
HHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
MSGPSEAYLMGLYGSRFTQADHDARMNALLWQRNAEAAVRQINNVSPAERAGFMARLSLI
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHH
QDTAPSSIGLSAPSGARSDAGYVYNLARYYRTNRNLPAAIDLLVNRPQFDAPALDPEVMI
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHH
GEMLRIAQGAGSDPAIRIASKVDDIFPAGADISGMSYRLRDDYTSLMWLGGTKALWNLAD
HHHHHHHHCCCCCCCEEHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEECCCHHHHCCCC
GSRAAPLFYRYGAAARTPPTRSKGFYWAGLAAEQAGQTSEARRYFEMAAGYPDRYYGQLA
CCCCCCHHEECCCCCCCCCCCCCCEEEECCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
LGKLGRDPQLANKAVPEPSFEQRAAFENASLTRAVREVARGAPWRTGIRFYQEIAQSADT
HHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PEEHALRAELAREIGRRDLAVNVAEAAGADGLNQFVAQGFPRLQTPPGVDWTMVHAISRQ
CHHHHHHHHHHHHHCCHHHEEEHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ESQFAENAISHAGARGLMQLMPGTAREQAGKLGMSYMQANLIESPSYNIQLGNGYFARMM
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
DYYGGAYPLAIAAYNAGPGNVNKWLRSNGDPRRGDIDYVTWIERIPIFETKNYVTRVIEN
HHCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
AAVYEHLYPDNAPMGRPRMARDFLR
HHHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
AQDAASWDRARSSLVAAQPSRMAQVIDRWDYLIGQDNLSFDQYAGFVLAYPDFPQADRL
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEECCCCCCCHHH
RVRAEEALDDEPIGPERLVAYFDQNPPLTNTARGRYALALANVGRPEAAEEARKAWRGGT
HHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
MSGPSEAYLMGLYGSRFTQADHDARMNALLWQRNAEAAVRQINNVSPAERAGFMARLSLI
CCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHH
QDTAPSSIGLSAPSGARSDAGYVYNLARYYRTNRNLPAAIDLLVNRPQFDAPALDPEVMI
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHHH
GEMLRIAQGAGSDPAIRIASKVDDIFPAGADISGMSYRLRDDYTSLMWLGGTKALWNLAD
HHHHHHHHCCCCCCCEEHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEECCCHHHHCCCC
GSRAAPLFYRYGAAARTPPTRSKGFYWAGLAAEQAGQTSEARRYFEMAAGYPDRYYGQLA
CCCCCCHHEECCCCCCCCCCCCCCEEEECCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHH
LGKLGRDPQLANKAVPEPSFEQRAAFENASLTRAVREVARGAPWRTGIRFYQEIAQSADT
HHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PEEHALRAELAREIGRRDLAVNVAEAAGADGLNQFVAQGFPRLQTPPGVDWTMVHAISRQ
CHHHHHHHHHHHHHCCHHHEEEHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ESQFAENAISHAGARGLMQLMPGTAREQAGKLGMSYMQANLIESPSYNIQLGNGYFARMM
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
DYYGGAYPLAIAAYNAGPGNVNKWLRSNGDPRRGDIDYVTWIERIPIFETKNYVTRVIEN
HHCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
AAVYEHLYPDNAPMGRPRMARDFLR
HHHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]