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The map label for this gene is trpA

Identifier: 85374346

GI number: 85374346

Start: 1572093

End: 1572872

Strand: Direct

Name: trpA

Synonym: ELI_07595

Alternate gene names: 85374346

Gene position: 1572093-1572872 (Clockwise)

Preceding gene: 162007984

Following gene: 85374347

Centisome position: 51.5

GC content: 67.56

Gene sequence:

>780_bases
ATGACCCGCCTCCAAAACGCCTTCTCCAAACCCCACGCCGCCTTCGTTGCCTTCATCACCGCTGGTGACGGGGACACGGC
GGCGAACCTTGATGCGCTGGTTGCGGGCGGTGCGGATGTGATCGAGTTGGGCATGCCCTTCACCGATCCCATGGCCGATG
GCCCCGCGATCCAGGCGGCCAATATCCGCTCGCTTGCGCGCGGCACGACCACGCGCGATGTGCTGATGATCGCCAACGAG
TTCCGCGAGCGCCACCCGGAGGTGCCGCTGGTATTGATGGGCTACGCCAACCCGATGGTGCGGCGCGGGCCGGAATGGTT
CGCCGCCGAGTGCAAGGGCTGCGGCGTCGATGGGGTGATTTGCGTCGATATCCCGCCCGAGGAAGACGCCGAGCTCGGCC
CCGCCCTGCGCGCAGCGGGCATCGCCCCGATCCGGCTCGCCACGCCCACCACCGATGCCAAGCGCCTGCCAGCAGTGCTC
GAGGGATGCGAAGGCTTCGTCTATTACGTTTCTGTCGCCGGGATCACCGGCAAGCAACAGGCCGCGCTCGACACGATCGA
AAGCAATGTGGCGCGGATCAAGCGATCGACCGACCTGCCCGTCGCGGTCGGCTTCGGCGTGCGCACGCCCGAACAGGCGG
GCGCTATCGCCAGGGTCGCGGACGGCGTGGTCGTCGGCTCCGCGCTGGTCGAACTGGTCGGCGAATTTGGCACCGAGGCC
CCGGCCAAGCTACAAGAGCTGACCAAGGCACTGGCTGACGCGGTCCATTCCGCACGCTAA

Upstream 100 bases:

>100_bases
GAGTTAATTATAACAAACCATTTTGGCGCGGCTATGCGCGTATCGTGACCACGAGAGATATCGCAACATGTGATCCAATT
CTTGGGTCTAGGGAAACTCA

Downstream 100 bases:

>100_bases
GGACGACAACATGAACTGGTTCACACGCGTTCGCAATTCGCTGGGCTTCGGCCCCAAGCGCACCACCGACAAGGATCTGT
GGGTCAAGTGCCCCTCCTGC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTRLQNAFSKPHAAFVAFITAGDGDTAANLDALVAGGADVIELGMPFTDPMADGPAIQAANIRSLARGTTTRDVLMIANE
FRERHPEVPLVLMGYANPMVRRGPEWFAAECKGCGVDGVICVDIPPEEDAELGPALRAAGIAPIRLATPTTDAKRLPAVL
EGCEGFVYYVSVAGITGKQQAALDTIESNVARIKRSTDLPVAVGFGVRTPEQAGAIARVADGVVVGSALVELVGEFGTEA
PAKLQELTKALADAVHSAR

Sequences:

>Translated_259_residues
MTRLQNAFSKPHAAFVAFITAGDGDTAANLDALVAGGADVIELGMPFTDPMADGPAIQAANIRSLARGTTTRDVLMIANE
FRERHPEVPLVLMGYANPMVRRGPEWFAAECKGCGVDGVICVDIPPEEDAELGPALRAAGIAPIRLATPTTDAKRLPAVL
EGCEGFVYYVSVAGITGKQQAALDTIESNVARIKRSTDLPVAVGFGVRTPEQAGAIARVADGVVVGSALVELVGEFGTEA
PAKLQELTKALADAVHSAR
>Mature_258_residues
TRLQNAFSKPHAAFVAFITAGDGDTAANLDALVAGGADVIELGMPFTDPMADGPAIQAANIRSLARGTTTRDVLMIANEF
RERHPEVPLVLMGYANPMVRRGPEWFAAECKGCGVDGVICVDIPPEEDAELGPALRAAGIAPIRLATPTTDAKRLPAVLE
GCEGFVYYVSVAGITGKQQAALDTIESNVARIKRSTDLPVAVGFGVRTPEQAGAIARVADGVVVGSALVELVGEFGTEAP
AKLQELTKALADAVHSAR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=266, Percent_Identity=33.4586466165414, Blast_Score=146, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321412, Length=234, Percent_Identity=40.1709401709402, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ERYLH (Q2N9N0)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458408.1
- HSSP:   P00929
- ProteinModelPortal:   Q2N9N0
- SMR:   Q2N9N0
- STRING:   Q2N9N0
- GeneID:   3871537
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07595
- NMPDR:   fig|314225.3.peg.1523
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q2N9N0
- ProtClustDB:   PRK13111
- BioCyc:   ELIT314225:ELI_07595-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 26974; Mature: 26843

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 42-42 ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLQNAFSKPHAAFVAFITAGDGDTAANLDALVAGGADVIELGMPFTDPMADGPAIQAA
CCCHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCEEHH
NIRSLARGTTTRDVLMIANEFRERHPEVPLVLMGYANPMVRRGPEWFAAECKGCGVDGVI
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCHHHHHCCCCCCCCEE
CVDIPPEEDAELGPALRAAGIAPIRLATPTTDAKRLPAVLEGCEGFVYYVSVAGITGKQQ
EEECCCCCCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHH
AALDTIESNVARIKRSTDLPVAVGFGVRTPEQAGAIARVADGVVVGSALVELVGEFGTEA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
PAKLQELTKALADAVHSAR
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRLQNAFSKPHAAFVAFITAGDGDTAANLDALVAGGADVIELGMPFTDPMADGPAIQAA
CCHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCEEHH
NIRSLARGTTTRDVLMIANEFRERHPEVPLVLMGYANPMVRRGPEWFAAECKGCGVDGVI
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCHHHHHCCCCCCCCEE
CVDIPPEEDAELGPALRAAGIAPIRLATPTTDAKRLPAVLEGCEGFVYYVSVAGITGKQQ
EEECCCCCCCCCCHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHH
AALDTIESNVARIKRSTDLPVAVGFGVRTPEQAGAIARVADGVVVGSALVELVGEFGTEA
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHCCCC
PAKLQELTKALADAVHSAR
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA