Click here to switch to the map view.

The map label for this gene is insK [C]

Identifier: 85374279

GI number: 85374279

Start: 1502557

End: 1503411

Strand: Direct

Name: insK [C]

Synonym: ELI_07260

Alternate gene names: 85374279

Gene position: 1502557-1503411 (Clockwise)

Preceding gene: 85374278

Following gene: 85374284

Centisome position: 49.23

GC content: 63.86

Gene sequence:

>855_bases
ATGAGCGAGCGGCGGGCGTGCCGTGTCATCGATGCCGATCGCAAGAGCGTGCGTTACCGTTCCACCCGGGGCGATGACGC
CGATCTGCGTGAGAAGCTGCGCGAGCTGGCCAACCAGCGACGGCGGTTCGGCTATCGCCGTCTGCACATCCTGCTGCGCC
GGGAGGGGATCATGATCAACCGGAAGAAGACCCAGAGGCTCTACAAGGAGGAAGGCTTGGCGGTGAGGCGTCGACGCAGT
CGCAGGCGCGCAGTCGGCACGAGGGCACCTGCTCCGGTGCTCGCCTTACCGAACCAGCGCTGGAGCCTGGACTTCGTGCA
CGACCAGATGGCGTCGGGCAGGAGGTTCCGGGTGCTCAACGTGGTCGATGACGTGACAAGGGAGTGTCTGGCAGCGGTGC
CGGACACGTCGATCTCTGGTCGCCGTGTCGTTCGAGAGCTGACCGAGCTGATCGCCCAGCGTGGCAAGCCCGGCATGATC
GTCAGCGACAACGGAACCGAGCTCACCAGCAACGCCGTGCTGGCATGGTGCGGCGAGATCGGCGTCGAGTGGCATTATAT
CGCGCCCGGAAGGCCGATGCAGAATGGCTATGTCGAGAGCTTCAACGGTCGCATGCGGGACGAACTGCTCAACGAGACGC
TGTTCATGAGCATGGCCCATGCCCGTGTCGAGATCGCCGCCTGGGTGGATGATTACAACCGGGAGCGACCGCACTCATCG
CTTGGCTACGCAACACCGGCGGCGTTCGCCGCCGAACTGGATAAGCAATGGCCTGCTTCGCTACGCCCTACGGGCTCCAC
TACGCAGCCCATTGCTTCAATCGCGCTTATGCGCAAAACAACCGCCCGGCTCTAA

Upstream 100 bases:

>100_bases
CGATGCTGGACAAAGCGGCGTTGAAGGATCTTCTGGCAAAAAAGTTCTGACGCCCGCCGCGCAGCGGGAAGCTGTCGCTC
ATCTTCAGGCATGCCACGGG

Downstream 100 bases:

>100_bases
TCCCAGCTGGGGGAAAGCTGGGGGTCACGTCATCGGGGAAAAGCGAGGTGTCGGCAGTCCAAATTTCACGCGGAGAGTCA
TGATAGCGTATAGGAAAAAC

Product: IS511, transposase OrfB

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYRRLHILLRREGIMINRKKTQRLYKEEGLAVRRRRS
RRRAVGTRAPAPVLALPNQRWSLDFVHDQMASGRRFRVLNVVDDVTRECLAAVPDTSISGRRVVRELTELIAQRGKPGMI
VSDNGTELTSNAVLAWCGEIGVEWHYIAPGRPMQNGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSS
LGYATPAAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL

Sequences:

>Translated_284_residues
MSERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYRRLHILLRREGIMINRKKTQRLYKEEGLAVRRRRS
RRRAVGTRAPAPVLALPNQRWSLDFVHDQMASGRRFRVLNVVDDVTRECLAAVPDTSISGRRVVRELTELIAQRGKPGMI
VSDNGTELTSNAVLAWCGEIGVEWHYIAPGRPMQNGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSS
LGYATPAAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL
>Mature_283_residues
SERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYRRLHILLRREGIMINRKKTQRLYKEEGLAVRRRRSR
RRAVGTRAPAPVLALPNQRWSLDFVHDQMASGRRFRVLNVVDDVTRECLAAVPDTSISGRRVVRELTELIAQRGKPGMIV
SDNGTELTSNAVLAWCGEIGVEWHYIAPGRPMQNGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSSL
GYATPAAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=239, Percent_Identity=26.3598326359833, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790724, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789424, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789224, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788305, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787669, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786558, Length=246, Percent_Identity=26.0162601626016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786462, Length=276, Percent_Identity=23.9130434782609, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32529; Mature: 32398

Theoretical pI: Translated: 11.27; Mature: 11.27

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYRRLHILLRREGIMIN
CCCCCHHHEECCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RKKTQRLYKEEGLAVRRRRSRRRAVGTRAPAPVLALPNQRWSLDFVHDQMASGRRFRVLN
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEHH
VVDDVTRECLAAVPDTSISGRRVVRELTELIAQRGKPGMIVSDNGTELTSNAVLAWCGEI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHH
GVEWHYIAPGRPMQNGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSS
CCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LGYATPAAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SERRACRVIDADRKSVRYRSTRGDDADLREKLRELANQRRRFGYRRLHILLRREGIMIN
CCCCHHHEECCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
RKKTQRLYKEEGLAVRRRRSRRRAVGTRAPAPVLALPNQRWSLDFVHDQMASGRRFRVLN
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEEHH
VVDDVTRECLAAVPDTSISGRRVVRELTELIAQRGKPGMIVSDNGTELTSNAVLAWCGEI
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHH
GVEWHYIAPGRPMQNGYVESFNGRMRDELLNETLFMSMAHARVEIAAWVDDYNRERPHSS
CCEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LGYATPAAFAAELDKQWPASLRPTGSTTQPIASIALMRKTTARL
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]