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The map label for this gene is ispDF

Identifier: 85374085

GI number: 85374085

Start: 1308988

End: 1310148

Strand: Direct

Name: ispDF

Synonym: ELI_06290

Alternate gene names: 85374085

Gene position: 1308988-1310148 (Clockwise)

Preceding gene: 85374077

Following gene: 85374086

Centisome position: 42.88

GC content: 65.03

Gene sequence:

>1161_bases
ATGGCCACGCCGAGTCCCCTCCCCTCCTTCGCTGCAATCGTCGTCGCCGCGGGCAAGGGACTGCGTGCGGGGCAGCCTGT
GCCGAAGCAGTTTGCGACCTGGCGTGGAAAGCCCGTGCTGCGGCACTCGGTCGAGAGTTTGCGCGCGGCAGGCGCCGACC
CTATCGTGGTGGCCATTCCAGACCACGCCGGGGACGTCGCGGCAAAAGCTCTCGACGGCATCACCGAAGTCGTATTCGTG
ACCGGAGGGCAAACGCGACAGGACTCGGTCCGGGCAGCGCTGGAAAGGCTGAGTTCCGTCCCGCCAGAACGGGTTCTGAT
CCATGACGCGGCCAGAGCCATCCTGCCGACACCGGTCATAGAGCGAGTGCTGCATGGCCTAGACGAAAGCGGCGGGGCCA
TTCCCGTACTCCCTGTCGTCGACAGCCTCGCCGTGGCAGCGGGAGAGCGCATGGCTGACAAGGCCGATCGCGAGAGCCTG
CGCCGGGTGCAGACCCCGCAAGGCTTTCGCTTTTCCGATATCCTGGCCGCACACCGCGCGTGGACCGGTGCCACCGATGC
CGGAGACGATGCGCAGGTCCTGATGGCGCACGGTGGCGACATCGCGCTCGTTGAAGGAGACGAAGCGCTCAAGAAGCTGA
CCTTTGCGGAGGATTTCATGGCAGATTTGCTCCCGGTGCGGGTCGGCACCGGCTTCGACGTACACAAGCTCGAAGCGGGC
GAGGAACTGTGGCTCGGCGGGATCAGGCTCGAACACGACAAAGGCTTGGCCGGTCACAGCGACGCCGATGTCGCGCTGCA
CGCGATCGTCGATGCCTTGTTGGGTGCGATCGGTAAGGGCGATATCGGCGATCATTTCCCGCCCAGCGATCCGCAATGGA
AGGGCGCGGCTTCTTCGGCCTTCATCGAGCACGCCGCGAAGCTTGTCGACGAGGCAGGCTACCGCGTCGGCAACATCGAC
CTCACCATCATATGCGAAGCGCCCAGGATCGGCCCGCATCGAGACGCCATGGGTCAGCGCATCGCAGAGCTCTTGGCGAC
ATCGCCCGATGCGATTAGCGTGAAGGCAACCACCACCGAAAAGCTCGGCTTTACCGGGCGCGGCGAAGGTATTGCGGCAC
AAGCCGCTGCGACCGTGGTCAGAAAGGACACCCCCGCATGA

Upstream 100 bases:

>100_bases
GGGAAGGAAGTTCGCTCATGCTGCGCTGCGATATGCCTAAAATTTGGGCAGCATCTAGTGGATTGCGAGCGCAGGCGCAA
GGCGGTAGCGCGCATGGCAC

Downstream 100 bases:

>100_bases
ACAAATCGCTCACAAAGCTGGCGAAAACCGCTGTCGCCTTGGCCGCACTGGCTGGCTCGCAGGCCGCGCAGGCACAAGCC
TGTATCGATCAGCAGGACCT

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MATPSPLPSFAAIVVAAGKGLRAGQPVPKQFATWRGKPVLRHSVESLRAAGADPIVVAIPDHAGDVAAKALDGITEVVFV
TGGQTRQDSVRAALERLSSVPPERVLIHDAARAILPTPVIERVLHGLDESGGAIPVLPVVDSLAVAAGERMADKADRESL
RRVQTPQGFRFSDILAAHRAWTGATDAGDDAQVLMAHGGDIALVEGDEALKKLTFAEDFMADLLPVRVGTGFDVHKLEAG
EELWLGGIRLEHDKGLAGHSDADVALHAIVDALLGAIGKGDIGDHFPPSDPQWKGAASSAFIEHAAKLVDEAGYRVGNID
LTIICEAPRIGPHRDAMGQRIAELLATSPDAISVKATTTEKLGFTGRGEGIAAQAAATVVRKDTPA

Sequences:

>Translated_386_residues
MATPSPLPSFAAIVVAAGKGLRAGQPVPKQFATWRGKPVLRHSVESLRAAGADPIVVAIPDHAGDVAAKALDGITEVVFV
TGGQTRQDSVRAALERLSSVPPERVLIHDAARAILPTPVIERVLHGLDESGGAIPVLPVVDSLAVAAGERMADKADRESL
RRVQTPQGFRFSDILAAHRAWTGATDAGDDAQVLMAHGGDIALVEGDEALKKLTFAEDFMADLLPVRVGTGFDVHKLEAG
EELWLGGIRLEHDKGLAGHSDADVALHAIVDALLGAIGKGDIGDHFPPSDPQWKGAASSAFIEHAAKLVDEAGYRVGNID
LTIICEAPRIGPHRDAMGQRIAELLATSPDAISVKATTTEKLGFTGRGEGIAAQAAATVVRKDTPA
>Mature_385_residues
ATPSPLPSFAAIVVAAGKGLRAGQPVPKQFATWRGKPVLRHSVESLRAAGADPIVVAIPDHAGDVAAKALDGITEVVFVT
GGQTRQDSVRAALERLSSVPPERVLIHDAARAILPTPVIERVLHGLDESGGAIPVLPVVDSLAVAAGERMADKADRESLR
RVQTPQGFRFSDILAAHRAWTGATDAGDDAQVLMAHGGDIALVEGDEALKKLTFAEDFMADLLPVRVGTGFDVHKLEAGE
ELWLGGIRLEHDKGLAGHSDADVALHAIVDALLGAIGKGDIGDHFPPSDPQWKGAASSAFIEHAAKLVDEAGYRVGNIDL
TIICEAPRIGPHRDAMGQRIAELLATSPDAISVKATTTEKLGFTGRGEGIAAQAAATVVRKDTPA

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=51.2820512820513, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789104, Length=217, Percent_Identity=33.1797235023041, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_ERYLH (Q2NAE1)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458147.1
- HSSP:   P62617
- ProteinModelPortal:   Q2NAE1
- SMR:   Q2NAE1
- STRING:   Q2NAE1
- GeneID:   3871177
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_06290
- NMPDR:   fig|314225.3.peg.1798
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q2NAE1
- ProtClustDB:   PRK09382
- BioCyc:   ELIT314225:ELI_06290-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40308; Mature: 40177

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPSPLPSFAAIVVAAGKGLRAGQPVPKQFATWRGKPVLRHSVESLRAAGADPIVVAIP
CCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEC
DHAGDVAAKALDGITEVVFVTGGQTRQDSVRAALERLSSVPPERVLIHDAARAILPTPVI
CCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
ERVLHGLDESGGAIPVLPVVDSLAVAAGERMADKADRESLRRVQTPQGFRFSDILAAHRA
HHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
WTGATDAGDDAQVLMAHGGDIALVEGDEALKKLTFAEDFMADLLPVRVGTGFDVHKLEAG
CCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCC
EELWLGGIRLEHDKGLAGHSDADVALHAIVDALLGAIGKGDIGDHFPPSDPQWKGAASSA
CEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
FIEHAAKLVDEAGYRVGNIDLTIICEAPRIGPHRDAMGQRIAELLATSPDAISVKATTTE
HHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHH
KLGFTGRGEGIAAQAAATVVRKDTPA
HCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATPSPLPSFAAIVVAAGKGLRAGQPVPKQFATWRGKPVLRHSVESLRAAGADPIVVAIP
CCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEC
DHAGDVAAKALDGITEVVFVTGGQTRQDSVRAALERLSSVPPERVLIHDAARAILPTPVI
CCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHH
ERVLHGLDESGGAIPVLPVVDSLAVAAGERMADKADRESLRRVQTPQGFRFSDILAAHRA
HHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
WTGATDAGDDAQVLMAHGGDIALVEGDEALKKLTFAEDFMADLLPVRVGTGFDVHKLEAG
CCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCC
EELWLGGIRLEHDKGLAGHSDADVALHAIVDALLGAIGKGDIGDHFPPSDPQWKGAASSA
CEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
FIEHAAKLVDEAGYRVGNIDLTIICEAPRIGPHRDAMGQRIAELLATSPDAISVKATTTE
HHHHHHHHHHHCCCEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHH
KLGFTGRGEGIAAQAAATVVRKDTPA
HCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA