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The map label for this gene is mazG [H]

Identifier: 85374077

GI number: 85374077

Start: 1298951

End: 1299700

Strand: Direct

Name: mazG [H]

Synonym: ELI_06250

Alternate gene names: 85374077

Gene position: 1298951-1299700 (Clockwise)

Preceding gene: 85374076

Following gene: 85374085

Centisome position: 42.56

GC content: 64.8

Gene sequence:

>750_bases
ATGACGACCCAGCTCGACCGCCTGCTTTCGATCATGGCCACCCTGCGCGATCCCGAGCGCGGCTGCGAATGGGATCTGGC
GCAGGATTTCAGCACCATCGCGCCCTATACGATCGAGGAAGCCTACGAAGTGGCTGACGCGATCGAGCGCGAGGCGATGG
ACGAATTGCGCGATGAACTGGGCGATCTCCTGCTGCAGGTCGTCTTTCACGCGCGCATGGCGGAGGAAGCCGGGCACTTC
GTTTTCGAGGATGTCGCGCGGTCGATTGCCGACAAGATGGAAGCGCGCCACCCGCACATTTTCGGCGGCGCGGACGGGCA
GATGACAGAGCGGCGCTGGGAAGAGCTCAAAGCCAAGGAACGCGACGCCAAGGGCGCGACAAGCGCAATGGACGGGGTCG
CGCGTGCCCTGCCCGCCCTGCTCCGCGCCGAGAAACTGCAGAAACGCGCCGCGCGCACCGGCTTCGACTGGCCCGACACC
GAAGGGCCCGCCGACAAGCTGCGTGAAGAAATGCAGGAACTGGCAGAAGCCCCCGCAGACAAGCGTGAGGAAGAGGCGGG
CGACCTGCTGTTCGCGGCGGTCAACCTCGTGCGTGCTTATGGCATCGCACCCGAAGACGCATTGCGCGCCGCTAATGGCA
AGTTCGAACGGCGCTTTCGCGGAATGGAGGGGAAAGCCGGCTCCACCTTCCCGCAGCTTTCGCTCGAGGAACAGGAAGCG
CTCTGGCAGGCGGTCAAGGCCGATGAATAG

Upstream 100 bases:

>100_bases
CGGGTTCGATCGAGCAGGCCCACCAACCCGATGAGTGGATCGCCCTCGAACAGCTCGGAAGGGGTGCCCGCTTCATGCAG
AATCTGGCACGGGAGCTGGC

Downstream 100 bases:

>100_bases
CGCGCGCTAAAGCGTCTCGTACTGGCCCAGTTCCTTGGGATTGAGCCGCACAGTCAGCTTGCGCATCGGGCCTTCGTCGC
CCTCGCCCCCATCCTCGTCC

Product: mazG family protein

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTTQLDRLLSIMATLRDPERGCEWDLAQDFSTIAPYTIEEAYEVADAIEREAMDELRDELGDLLLQVVFHARMAEEAGHF
VFEDVARSIADKMEARHPHIFGGADGQMTERRWEELKAKERDAKGATSAMDGVARALPALLRAEKLQKRAARTGFDWPDT
EGPADKLREEMQELAEAPADKREEEAGDLLFAAVNLVRAYGIAPEDALRAANGKFERRFRGMEGKAGSTFPQLSLEEQEA
LWQAVKADE

Sequences:

>Translated_249_residues
MTTQLDRLLSIMATLRDPERGCEWDLAQDFSTIAPYTIEEAYEVADAIEREAMDELRDELGDLLLQVVFHARMAEEAGHF
VFEDVARSIADKMEARHPHIFGGADGQMTERRWEELKAKERDAKGATSAMDGVARALPALLRAEKLQKRAARTGFDWPDT
EGPADKLREEMQELAEAPADKREEEAGDLLFAAVNLVRAYGIAPEDALRAANGKFERRFRGMEGKAGSTFPQLSLEEQEA
LWQAVKADE
>Mature_248_residues
TTQLDRLLSIMATLRDPERGCEWDLAQDFSTIAPYTIEEAYEVADAIEREAMDELRDELGDLLLQVVFHARMAEEAGHFV
FEDVARSIADKMEARHPHIFGGADGQMTERRWEELKAKERDAKGATSAMDGVARALPALLRAEKLQKRAARTGFDWPDTE
GPADKLREEMQELAEAPADKREEEAGDLLFAAVNLVRAYGIAPEDALRAANGKFERRFRGMEGKAGSTFPQLSLEEQEAL
WQAVKADE

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=260, Percent_Identity=52.6923076923077, Blast_Score=250, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 27911; Mature: 27779

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQLDRLLSIMATLRDPERGCEWDLAQDFSTIAPYTIEEAYEVADAIEREAMDELRDEL
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
GDLLLQVVFHARMAEEAGHFVFEDVARSIADKMEARHPHIFGGADGQMTERRWEELKAKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHH
RDAKGATSAMDGVARALPALLRAEKLQKRAARTGFDWPDTEGPADKLREEMQELAEAPAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
KREEEAGDLLFAAVNLVRAYGIAPEDALRAANGKFERRFRGMEGKAGSTFPQLSLEEQEA
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCHHHHHH
LWQAVKADE
HHHHHHCCC
>Mature Secondary Structure 
TTQLDRLLSIMATLRDPERGCEWDLAQDFSTIAPYTIEEAYEVADAIEREAMDELRDEL
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
GDLLLQVVFHARMAEEAGHFVFEDVARSIADKMEARHPHIFGGADGQMTERRWEELKAKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHH
RDAKGATSAMDGVARALPALLRAEKLQKRAARTGFDWPDTEGPADKLREEMQELAEAPAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
KREEEAGDLLFAAVNLVRAYGIAPEDALRAANGKFERRFRGMEGKAGSTFPQLSLEEQEA
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCHHHHHH
LWQAVKADE
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]