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The map label for this gene is gshB [H]

Identifier: 85374025

GI number: 85374025

Start: 1239786

End: 1240739

Strand: Direct

Name: gshB [H]

Synonym: ELI_05990

Alternate gene names: 85374025

Gene position: 1239786-1240739 (Clockwise)

Preceding gene: 85374024

Following gene: 85374030

Centisome position: 40.62

GC content: 61.64

Gene sequence:

>954_bases
ATGGCCTTGAAAGTCGCCGTCCAGATGGACCCGATGGAAAGCATCAACATCGGTGGCGATTCCAGCTTCGCCCTGATGCT
GGCTGCGCAGGCGCGGGGGCACACGGTCTATCACTACGACGTGGGCTCGCTCGCCTACGAGGATGGCAGGATCACCGCCC
ATTGCGCACCGGTGACCGTCCAGCCCGTCGAAGGCGATCACTTCACGATGGGAGAGCGCCGCAAGCTGGACCTCGCGCAG
GACATCGACGTGGTGCTGATGCGGCAGGACCCGCCCTTTCACTTGGGCTACATTTCCGCCGCATTGCTGCTGGATCGACT
GAAGGGCACCACACTCGTCGTCAACGATCCGCGCGAAGTCATCAATGCGCCCGAAAAAATGTTCGTGCTCGATTACGCTC
GTTTCATGCCACCGACCCTCATTGCCCGCCAACTGTCGGACATTCGCGAATTCCAGGCAAAACACGGGGCCATTGTTGTC
AAGCCCCTCCACGGCAACGGCGGCAAGGCGATCTTCAAGGTCGAGGCCGATGGCGGCAATCTTTCGGCGCTGAGCGAAGT
TTTCAACCAGACCTGGCCCGAAGCCCACATGGTGCAGCCGTTCCTGCCGGAAGTGGCCGAAGGCGACAAACGTATCGTCC
TCGTCGATGGTGAGTTCACGGGCGCGATCAATCGCAAGCCGGGCGAGGGCGAGTTTCGCTCAAACCTCGCGCAGGGCGGC
TATGCCGAAGCGGCCGGCCTCACGGAGCGGGAAGAAGAAATCTGCGCGACGATGGGCCCCGAGCTGAAACGGCGCGGGTT
GGTGTTCGTGGGCATCGATGTGATCGGCGGCAAGTATCTCACCGAGATCAATGTCACTTCGCCCACCGGCATCGTCGCGA
TTGACAAGTTCAACGGCACCGACACGCCAGCAAAAATCTGGGACGCGATCGAAGCGCGCCACGCGGCGATGTAG

Upstream 100 bases:

>100_bases
ATGCGCAGGACGGCGAGGATTATCGCATCGACGTGATCCTCCTTGCACCCGGCAGCTTCCCGCGCCACATCGCCAACGCC
TGGCAACCTTAGGAAAGAAC

Downstream 100 bases:

>100_bases
CTAATCCTTCTCGCGCGGATGCGCTTTGCGATAGGTGTCAAGCAGGGTGGCCGCATCCACCTTGGTATAGATCTGCGTCG
AACCGAGACTGGCGTGGCCG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MALKVAVQMDPMESINIGGDSSFALMLAAQARGHTVYHYDVGSLAYEDGRITAHCAPVTVQPVEGDHFTMGERRKLDLAQ
DIDVVLMRQDPPFHLGYISAALLLDRLKGTTLVVNDPREVINAPEKMFVLDYARFMPPTLIARQLSDIREFQAKHGAIVV
KPLHGNGGKAIFKVEADGGNLSALSEVFNQTWPEAHMVQPFLPEVAEGDKRIVLVDGEFTGAINRKPGEGEFRSNLAQGG
YAEAAGLTEREEEICATMGPELKRRGLVFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGTDTPAKIWDAIEARHAAM

Sequences:

>Translated_317_residues
MALKVAVQMDPMESINIGGDSSFALMLAAQARGHTVYHYDVGSLAYEDGRITAHCAPVTVQPVEGDHFTMGERRKLDLAQ
DIDVVLMRQDPPFHLGYISAALLLDRLKGTTLVVNDPREVINAPEKMFVLDYARFMPPTLIARQLSDIREFQAKHGAIVV
KPLHGNGGKAIFKVEADGGNLSALSEVFNQTWPEAHMVQPFLPEVAEGDKRIVLVDGEFTGAINRKPGEGEFRSNLAQGG
YAEAAGLTEREEEICATMGPELKRRGLVFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGTDTPAKIWDAIEARHAAM
>Mature_316_residues
ALKVAVQMDPMESINIGGDSSFALMLAAQARGHTVYHYDVGSLAYEDGRITAHCAPVTVQPVEGDHFTMGERRKLDLAQD
IDVVLMRQDPPFHLGYISAALLLDRLKGTTLVVNDPREVINAPEKMFVLDYARFMPPTLIARQLSDIREFQAKHGAIVVK
PLHGNGGKAIFKVEADGGNLSALSEVFNQTWPEAHMVQPFLPEVAEGDKRIVLVDGEFTGAINRKPGEGEFRSNLAQGGY
AEAAGLTEREEEICATMGPELKRRGLVFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGTDTPAKIWDAIEARHAAM

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=313, Percent_Identity=40.2555910543131, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1787076, Length=186, Percent_Identity=32.258064516129, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34524; Mature: 34393

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKVAVQMDPMESINIGGDSSFALMLAAQARGHTVYHYDVGSLAYEDGRITAHCAPVTV
CEEEEEEEECCCCEECCCCCCCEEEEEEECCCCCEEEEEECCCEEEECCEEEEEECCEEE
QPVEGDHFTMGERRKLDLAQDIDVVLMRQDPPFHLGYISAALLLDRLKGTTLVVNDPREV
EECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHCCCCEEEEECCHHH
INAPEKMFVLDYARFMPPTLIARQLSDIREFQAKHGAIVVKPLHGNGGKAIFKVEADGGN
HCCCHHEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEEECCCCC
LSALSEVFNQTWPEAHMVQPFLPEVAEGDKRIVLVDGEFTGAINRKPGEGEFRSNLAQGG
HHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCC
YAEAAGLTEREEEICATMGPELKRRGLVFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGT
CCHHCCCCHHHHHHHHHCCHHHHHCCEEEEEEEEECCEEEEEEEECCCCCEEEEECCCCC
DTPAKIWDAIEARHAAM
CCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALKVAVQMDPMESINIGGDSSFALMLAAQARGHTVYHYDVGSLAYEDGRITAHCAPVTV
EEEEEEEECCCCEECCCCCCCEEEEEEECCCCCEEEEEECCCEEEECCEEEEEECCEEE
QPVEGDHFTMGERRKLDLAQDIDVVLMRQDPPFHLGYISAALLLDRLKGTTLVVNDPREV
EECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHCCCCEEEEECCHHH
INAPEKMFVLDYARFMPPTLIARQLSDIREFQAKHGAIVVKPLHGNGGKAIFKVEADGGN
HCCCHHEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEEECCCCC
LSALSEVFNQTWPEAHMVQPFLPEVAEGDKRIVLVDGEFTGAINRKPGEGEFRSNLAQGG
HHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHCCC
YAEAAGLTEREEEICATMGPELKRRGLVFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGT
CCHHCCCCHHHHHHHHHCCHHHHHCCEEEEEEEEECCEEEEEEEECCCCCEEEEECCCCC
DTPAKIWDAIEARHAAM
CCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA