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The map label for this gene is rnhB

Identifier: 85373281

GI number: 85373281

Start: 493323

End: 493961

Strand: Direct

Name: rnhB

Synonym: ELI_02270

Alternate gene names: 85373281

Gene position: 493323-493961 (Clockwise)

Preceding gene: 85373274

Following gene: 85373282

Centisome position: 16.16

GC content: 64.32

Gene sequence:

>639_bases
ATGGTTTCTGAAACTCCCGAAGATGCAATCGTTTCCCGGCCCGAAGCGCAAGGATGCGTGTTCGGCGTCGACGAGGCCGG
GCGCGGGCCCTTGGCCGGTCCGGTCGTCGCCGCCGCAGTGGTGCTGGGAGATGCCTACCCCAAGGGGCTCGACGATTCCA
AGAAGCTGACCGCCAAACGACGCGCGGTGCTCGACGAAGATATCCGCAAGACATGCGGCTGGGGGGTCGGCGTGGTCGAT
GTCGAAGAGATCGATACGCTCAATATCCTCGGCGCGACCATGCGGGCGATGAGCCTCGCGGTACAGGGACTTGCAGCCAA
GCTTGGTGGCGATCCCGATATGGTGTTGATCGATGGCAACCTGACCCCGCATGGCCGCTGCGACCTCTGGCAATGGGAGG
CGCAGGCGATCATCGGCGGTGACGGGATCGAGCCGGCGATCTCCGCTGCCAGCATCGTCGCCAAGGAGTGGCGCGACCGG
ATCATGACCGAGGCGGCGCAGGACTATCCCGAATACGGCTGGCTTTCGAACAAGGGTTACGGAACCGCGCAGCACCTCGA
AGCGCTAAGACTGCATGGACCGACGCCGCTGCACCGCCGCAGCTTTGCGCCGGTCGCGCAGCAGGAGCTATTTCGATGA

Upstream 100 bases:

>100_bases
CTGCGCTTCGGCGCTATCCCCCGAATAGGCGCTGCCGCAGCTTGCGAGGGCGATGGCCGGGAGCGACAGAAGGGCGAGCG
ATGTGAAGCGTGTCTGGATC

Downstream 100 bases:

>100_bases
GCGCTTTCGCCTATTCCGACATTCCCGACCAGTCCGGCAAGACCGCCATCGTAACCGGTGCCAATACCGGATTGGGCTAT
TGTATCGCCGAAGCGCTGGC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MVSETPEDAIVSRPEAQGCVFGVDEAGRGPLAGPVVAAAVVLGDAYPKGLDDSKKLTAKRRAVLDEDIRKTCGWGVGVVD
VEEIDTLNILGATMRAMSLAVQGLAAKLGGDPDMVLIDGNLTPHGRCDLWQWEAQAIIGGDGIEPAISAASIVAKEWRDR
IMTEAAQDYPEYGWLSNKGYGTAQHLEALRLHGPTPLHRRSFAPVAQQELFR

Sequences:

>Translated_212_residues
MVSETPEDAIVSRPEAQGCVFGVDEAGRGPLAGPVVAAAVVLGDAYPKGLDDSKKLTAKRRAVLDEDIRKTCGWGVGVVD
VEEIDTLNILGATMRAMSLAVQGLAAKLGGDPDMVLIDGNLTPHGRCDLWQWEAQAIIGGDGIEPAISAASIVAKEWRDR
IMTEAAQDYPEYGWLSNKGYGTAQHLEALRLHGPTPLHRRSFAPVAQQELFR
>Mature_212_residues
MVSETPEDAIVSRPEAQGCVFGVDEAGRGPLAGPVVAAAVVLGDAYPKGLDDSKKLTAKRRAVLDEDIRKTCGWGVGVVD
VEEIDTLNILGATMRAMSLAVQGLAAKLGGDPDMVLIDGNLTPHGRCDLWQWEAQAIIGGDGIEPAISAASIVAKEWRDR
IMTEAAQDYPEYGWLSNKGYGTAQHLEALRLHGPTPLHRRSFAPVAQQELFR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=196, Percent_Identity=51.0204081632653, Blast_Score=159, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ERYLH (Q2NCP5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457343.1
- ProteinModelPortal:   Q2NCP5
- SMR:   Q2NCP5
- STRING:   Q2NCP5
- GeneID:   3869116
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_02270
- NMPDR:   fig|314225.3.peg.2634
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q2NCP5
- ProtClustDB:   PRK00015
- BioCyc:   ELIT314225:ELI_02270-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22713; Mature: 22713

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSETPEDAIVSRPEAQGCVFGVDEAGRGPLAGPVVAAAVVLGDAYPKGLDDSKKLTAKR
CCCCCCCHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RAVLDEDIRKTCGWGVGVVDVEEIDTLNILGATMRAMSLAVQGLAAKLGGDPDMVLIDGN
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
LTPHGRCDLWQWEAQAIIGGDGIEPAISAASIVAKEWRDRIMTEAAQDYPEYGWLSNKGY
CCCCCCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GTAQHLEALRLHGPTPLHRRSFAPVAQQELFR
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MVSETPEDAIVSRPEAQGCVFGVDEAGRGPLAGPVVAAAVVLGDAYPKGLDDSKKLTAKR
CCCCCCCHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RAVLDEDIRKTCGWGVGVVDVEEIDTLNILGATMRAMSLAVQGLAAKLGGDPDMVLIDGN
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
LTPHGRCDLWQWEAQAIIGGDGIEPAISAASIVAKEWRDRIMTEAAQDYPEYGWLSNKGY
CCCCCCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GTAQHLEALRLHGPTPLHRRSFAPVAQQELFR
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA