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The map label for this gene is def

Identifier: 85373243

GI number: 85373243

Start: 459929

End: 460513

Strand: Direct

Name: def

Synonym: ELI_02080

Alternate gene names: 85373243

Gene position: 459929-460513 (Clockwise)

Preceding gene: 85373242

Following gene: 85373245

Centisome position: 15.07

GC content: 59.83

Gene sequence:

>585_bases
ATGGCTATTCGTGAAATCCTGGAAGTGCCGGATCCCCGGCTCAAGACCGTGTCCGAACCTGTCCAGCCGGACGAGTTCAA
CGACGATCTCAAGCAGCTGGTCGATGACATGTTCGAAACGATGTATGCCGCGCCCGGCATCGGCTTGGCCGCAATCCAGG
TCGGCGTGCCAAAGCGGGTGCTGGTGATCGACCTGCAGGAGCCCGACATGGATGCCGAGCCCGAGGAATGCGGCCATGAC
CACGGCGACGGGGAAGGCGCGCACAAGCATTATCCGGTCAAGAACGACCCGCGCATCTTCATCAACCCGGAAATCATTGA
TCCGAACGAGGAACTGAGCACCTATCAGGAAGGCTGCCTCTCGGTCCCTGAGATATACGCCGACGTCGACCGGCCGAAGA
CCTGCACGGTCAAGTACCAGGACCTCACCGGAAAGACGCACCAGGAAGACCTCGACGGCCTGCTGGCGACCTGCCTGCAG
CATGAGATGGATCACTTGGAGGGCATCCTCTTCATCGACCACCTCAGCCGGTTGAAGAAGCAGATGGCGCTGAAGAAACT
CAAGAAGATGCGCCAAGCCGCCTGA

Upstream 100 bases:

>100_bases
CAGTAATGCCGCTGAGGGCGCGGTGGCCGTTTGGCTAGGCGGTCACTCTGATCGGAAAGGCTGGCTTGCAGCCCGCCGCC
CCAGCGCCTAAATTACGCGC

Downstream 100 bases:

>100_bases
GCGGTCGCGAGTTTCGACAGCATCAAAAAGCCCCGCTTTTAAGCGGGGCTTTTCTTTCCAGGCTTTGTGGCGGTCTTATT
CGCCGCGCTGTTCCATGCCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MAIREILEVPDPRLKTVSEPVQPDEFNDDLKQLVDDMFETMYAAPGIGLAAIQVGVPKRVLVIDLQEPDMDAEPEECGHD
HGDGEGAHKHYPVKNDPRIFINPEIIDPNEELSTYQEGCLSVPEIYADVDRPKTCTVKYQDLTGKTHQEDLDGLLATCLQ
HEMDHLEGILFIDHLSRLKKQMALKKLKKMRQAA

Sequences:

>Translated_194_residues
MAIREILEVPDPRLKTVSEPVQPDEFNDDLKQLVDDMFETMYAAPGIGLAAIQVGVPKRVLVIDLQEPDMDAEPEECGHD
HGDGEGAHKHYPVKNDPRIFINPEIIDPNEELSTYQEGCLSVPEIYADVDRPKTCTVKYQDLTGKTHQEDLDGLLATCLQ
HEMDHLEGILFIDHLSRLKKQMALKKLKKMRQAA
>Mature_193_residues
AIREILEVPDPRLKTVSEPVQPDEFNDDLKQLVDDMFETMYAAPGIGLAAIQVGVPKRVLVIDLQEPDMDAEPEECGHDH
GDGEGAHKHYPVKNDPRIFINPEIIDPNEELSTYQEGCLSVPEIYADVDRPKTCTVKYQDLTGKTHQEDLDGLLATCLQH
EMDHLEGILFIDHLSRLKKQMALKKLKKMRQAA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=171, Percent_Identity=32.1637426900585, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1789682, Length=190, Percent_Identity=40, Blast_Score=137, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645728, Length=173, Percent_Identity=27.7456647398844, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24645726, Length=171, Percent_Identity=29.2397660818713, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_ERYLH (Q2NCT3)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457305.1
- ProteinModelPortal:   Q2NCT3
- SMR:   Q2NCT3
- STRING:   Q2NCT3
- GeneID:   3869774
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_02080
- NMPDR:   fig|314225.3.peg.2672
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- PhylomeDB:   Q2NCT3
- ProtClustDB:   PRK00150
- BioCyc:   ELIT314225:ELI_02080-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21910; Mature: 21779

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIREILEVPDPRLKTVSEPVQPDEFNDDLKQLVDDMFETMYAAPGIGLAAIQVGVPKRV
CCHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCEE
LVIDLQEPDMDAEPEECGHDHGDGEGAHKHYPVKNDPRIFINPEIIDPNEELSTYQEGCL
EEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCEECCCHHHHHHHHHCC
SVPEIYADVDRPKTCTVKYQDLTGKTHQEDLDGLLATCLQHEMDHLEGILFIDHLSRLKK
CHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMALKKLKKMRQAA
HHHHHHHHHHHHCC
>Mature Secondary Structure 
AIREILEVPDPRLKTVSEPVQPDEFNDDLKQLVDDMFETMYAAPGIGLAAIQVGVPKRV
CHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCEE
LVIDLQEPDMDAEPEECGHDHGDGEGAHKHYPVKNDPRIFINPEIIDPNEELSTYQEGCL
EEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCEECCCHHHHHHHHHCC
SVPEIYADVDRPKTCTVKYQDLTGKTHQEDLDGLLATCLQHEMDHLEGILFIDHLSRLKK
CHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QMALKKLKKMRQAA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA