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The map label for this gene is serA [H]

Identifier: 85373221

GI number: 85373221

Start: 437366

End: 438949

Strand: Direct

Name: serA [H]

Synonym: ELI_01970

Alternate gene names: 85373221

Gene position: 437366-438949 (Clockwise)

Preceding gene: 85373219

Following gene: 85373222

Centisome position: 14.33

GC content: 64.27

Gene sequence:

>1584_bases
ATGACCAAGCCCAAAGTCCTCATCTCCGACAAGATGGACTCGAACGCCGCCCGCATCTTCGAAGAGCGCGGCTGCGATGT
CGACGTGATCACCGGCGAATCACCCGAGGAGCTGAGGGCCCGCATCGGCGAATACGAAGGCCTTGCCATCCGTTCCTCGA
CCAAGGTGACGCCCGAAATCCTCGATGCGGCGACCAACCTCAAGGTCATCGGTCGCGCCGGGATCGGCGTGGATAACGTC
GATATTCCGTACGCCAGCTCCAAGGGCGTGGTGGTGATGAATACGCCGTTCGGCAATTCGATCACCACGGCCGAACATGC
GGTGGCGATGATGTTCGCGCTCGCGCGCCAGATTCCGCAGGCCGACCGGCGTACGCAGGCGGGCGAATGGCCCAAGAACG
ACTTCATGGGCGTGGAACTGACCGGCAAGATCCTGGGCCTGATCGGAGCCGGCAATATCGGTTCGATCGTCGCCGCGCGG
GCGCAGGGCCTGAAGATGAAGGTCGTCGCCTTCGATCCGTTCCTGACCGAGGAGCGCGCGATCGAGATCGGGGTCGAAAA
GGTCGATCTCGATTCGCTGCTGGCACGGGCCGACTTCATTACGCTGCACACCCCGCTGACCGACGAGACGCGCAACATCC
TCTCGCAAGAGAATCTCGCGAAGACCAAGCAGGGCGTGCGCATCATCAATTGCGCGCGCGGGGGCCTGATCGACGAGGCG
GCGCTGGCCGAGGCGCTCGACAGCGGCCAGGTTGCGGGAGCGGCGCTGGACGTGTTCCAGACCGAACCGGCCAAGGACAG
CCCGCTGTTCGGCAAGCCCAACTTCATCTGCACGCCGCACCTTGGCGCTTCGACCACCGAGGCGCAGGTCAATGTCGCGC
TGCAGGTGGCCGAGCAGATGGCCGATTATCTCGTCGATGGCGGCGTCACCAATGCGCTGAACATGCCGTCGCTCAGCGCA
GAGGAAGCGCCCAAGCTCAAGCCCTACATGGGTCTGGCGGAAAACCTCGGCAGCCTCGTCGGCCAGCTGGCGCACGGCAA
TCTCACCAAAATCAGCATCGAACGCGAAGGCGCGGCGGCGGACCTTTCCGGCAAACCGATCGAAGGTGCGGTGCTGGCCG
GGCTGATGCGGCAGTATTCGGACACGGTGAACATGGTCAACGCACCCTTCCTTGCCAAGGAACGCGGTCTCGACATTCGC
TCCATCCGCCACGAGAAGGAAGGCGCCTACAACACCTTGCTGCGCGTGACGGTGGGCACGGAGCAGGGCGACCGCTCGGT
CGCCGGAACGCTATTCGGCAGCGATGCCCCGCGACTGGTCGAGATTTTCGGCGTCCGGATCGAGGCTCAGCTGGAAGGCC
ACATGCTCTACATCGTCAACGAGGACGCGCCGGGCTTCATCGGCCGCATCGGAACGCTGCTGGGCGAGCACGGCATCAAT
ATCGGCACCTTCAATCTCGGGCGCCGCAGTGCCGGGGGGGAGGCCGTGCTGCTGCTTAGCGTCGACCAGCCGATCCCGCA
GGATGTCGTCAAGGCGGCCTGCGCGCTCGACGGCGTGAAAACGGTGATGCCGCTTTCTTTCTGA

Upstream 100 bases:

>100_bases
CGAAGCGCCGCCCTTACTTTTGGCGAGCGCTGTACCCGAAGAAAGTGCAGCCCTTCGACAGGCTCAGGGCGAACGGTTTC
AGTTTTCTGGAGTTATTCCA

Downstream 100 bases:

>100_bases
CCTTCCACGTCACCCCGTCGGAAGCTGGGGGCGGGCGGCATCGGGTCCGACTTGATTGAGAAAGATCCCAGCTTCCGCTG
GGATGACGAGGTATGTATGG

Product: D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: PGDH [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTKPKVLISDKMDSNAARIFEERGCDVDVITGESPEELRARIGEYEGLAIRSSTKVTPEILDAATNLKVIGRAGIGVDNV
DIPYASSKGVVVMNTPFGNSITTAEHAVAMMFALARQIPQADRRTQAGEWPKNDFMGVELTGKILGLIGAGNIGSIVAAR
AQGLKMKVVAFDPFLTEERAIEIGVEKVDLDSLLARADFITLHTPLTDETRNILSQENLAKTKQGVRIINCARGGLIDEA
ALAEALDSGQVAGAALDVFQTEPAKDSPLFGKPNFICTPHLGASTTEAQVNVALQVAEQMADYLVDGGVTNALNMPSLSA
EEAPKLKPYMGLAENLGSLVGQLAHGNLTKISIEREGAAADLSGKPIEGAVLAGLMRQYSDTVNMVNAPFLAKERGLDIR
SIRHEKEGAYNTLLRVTVGTEQGDRSVAGTLFGSDAPRLVEIFGVRIEAQLEGHMLYIVNEDAPGFIGRIGTLLGEHGIN
IGTFNLGRRSAGGEAVLLLSVDQPIPQDVVKAACALDGVKTVMPLSF

Sequences:

>Translated_527_residues
MTKPKVLISDKMDSNAARIFEERGCDVDVITGESPEELRARIGEYEGLAIRSSTKVTPEILDAATNLKVIGRAGIGVDNV
DIPYASSKGVVVMNTPFGNSITTAEHAVAMMFALARQIPQADRRTQAGEWPKNDFMGVELTGKILGLIGAGNIGSIVAAR
AQGLKMKVVAFDPFLTEERAIEIGVEKVDLDSLLARADFITLHTPLTDETRNILSQENLAKTKQGVRIINCARGGLIDEA
ALAEALDSGQVAGAALDVFQTEPAKDSPLFGKPNFICTPHLGASTTEAQVNVALQVAEQMADYLVDGGVTNALNMPSLSA
EEAPKLKPYMGLAENLGSLVGQLAHGNLTKISIEREGAAADLSGKPIEGAVLAGLMRQYSDTVNMVNAPFLAKERGLDIR
SIRHEKEGAYNTLLRVTVGTEQGDRSVAGTLFGSDAPRLVEIFGVRIEAQLEGHMLYIVNEDAPGFIGRIGTLLGEHGIN
IGTFNLGRRSAGGEAVLLLSVDQPIPQDVVKAACALDGVKTVMPLSF
>Mature_526_residues
TKPKVLISDKMDSNAARIFEERGCDVDVITGESPEELRARIGEYEGLAIRSSTKVTPEILDAATNLKVIGRAGIGVDNVD
IPYASSKGVVVMNTPFGNSITTAEHAVAMMFALARQIPQADRRTQAGEWPKNDFMGVELTGKILGLIGAGNIGSIVAARA
QGLKMKVVAFDPFLTEERAIEIGVEKVDLDSLLARADFITLHTPLTDETRNILSQENLAKTKQGVRIINCARGGLIDEAA
LAEALDSGQVAGAALDVFQTEPAKDSPLFGKPNFICTPHLGASTTEAQVNVALQVAEQMADYLVDGGVTNALNMPSLSAE
EAPKLKPYMGLAENLGSLVGQLAHGNLTKISIEREGAAADLSGKPIEGAVLAGLMRQYSDTVNMVNAPFLAKERGLDIRS
IRHEKEGAYNTLLRVTVGTEQGDRSVAGTLFGSDAPRLVEIFGVRIEAQLEGHMLYIVNEDAPGFIGRIGTLLGEHGINI
GTFNLGRRSAGGEAVLLLSVDQPIPQDVVKAACALDGVKTVMPLSF

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=399, Percent_Identity=44.1102756892231, Blast_Score=339, Evalue=5e-93,
Organism=Homo sapiens, GI61743967, Length=334, Percent_Identity=31.437125748503, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI4557497, Length=334, Percent_Identity=31.437125748503, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI145580578, Length=301, Percent_Identity=32.890365448505, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI4557499, Length=301, Percent_Identity=32.890365448505, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI145580575, Length=279, Percent_Identity=34.4086021505376, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI6912396, Length=312, Percent_Identity=30.1282051282051, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1789279, Length=291, Percent_Identity=35.7388316151203, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI87082289, Length=319, Percent_Identity=30.7210031347962, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787645, Length=196, Percent_Identity=36.734693877551, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081824, Length=235, Percent_Identity=28.0851063829787, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788660, Length=269, Percent_Identity=28.6245353159851, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=306, Percent_Identity=44.1176470588235, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI25147481, Length=311, Percent_Identity=30.5466237942122, Blast_Score=143, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320925, Length=329, Percent_Identity=36.4741641337386, Blast_Score=216, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6322116, Length=328, Percent_Identity=36.280487804878, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6324055, Length=265, Percent_Identity=29.4339622641509, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324964, Length=322, Percent_Identity=27.0186335403727, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6325144, Length=123, Percent_Identity=38.2113821138211, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324980, Length=201, Percent_Identity=28.3582089552239, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19921140, Length=300, Percent_Identity=45, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI28574286, Length=304, Percent_Identity=29.6052631578947, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646446, Length=313, Percent_Identity=29.073482428115, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24646448, Length=313, Percent_Identity=29.073482428115, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24646452, Length=313, Percent_Identity=29.073482428115, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24646450, Length=313, Percent_Identity=29.073482428115, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI62472511, Length=313, Percent_Identity=29.073482428115, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24585516, Length=306, Percent_Identity=27.1241830065359, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28574284, Length=271, Percent_Identity=27.6752767527675, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24585514, Length=271, Percent_Identity=27.6752767527675, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28574282, Length=271, Percent_Identity=27.6752767527675, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45552429, Length=264, Percent_Identity=28.030303030303, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI45551003, Length=271, Percent_Identity=27.6752767527675, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI28571528, Length=276, Percent_Identity=31.1594202898551, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT [H]

EC number: =1.1.1.95 [H]

Molecular weight: Translated: 55964; Mature: 55833

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPKVLISDKMDSNAARIFEERGCDVDVITGESPEELRARIGEYEGLAIRSSTKVTPEI
CCCCCEEEECCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCHHH
LDAATNLKVIGRAGIGVDNVDIPYASSKGVVVMNTPFGNSITTAEHAVAMMFALARQIPQ
HHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
ADRRTQAGEWPKNDFMGVELTGKILGLIGAGNIGSIVAARAQGLKMKVVAFDPFLTEERA
HHHHCCCCCCCCCCCEEEEEECEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
IEIGVEKVDLDSLLARADFITLHTPLTDETRNILSQENLAKTKQGVRIINCARGGLIDEA
EEECCEEECHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
ALAEALDSGQVAGAALDVFQTEPAKDSPLFGKPNFICTPHLGASTTEAQVNVALQVAEQM
HHHHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
ADYLVDGGVTNALNMPSLSAEEAPKLKPYMGLAENLGSLVGQLAHGNLTKISIEREGAAA
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
DLSGKPIEGAVLAGLMRQYSDTVNMVNAPFLAKERGLDIRSIRHEKEGAYNTLLRVTVGT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCCCCEEEEEEEECC
EQGDRSVAGTLFGSDAPRLVEIFGVRIEAQLEGHMLYIVNEDAPGFIGRIGTLLGEHGIN
CCCCCCEEEEECCCCCCCEEHHCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHCCCE
IGTFNLGRRSAGGEAVLLLSVDQPIPQDVVKAACALDGVKTVMPLSF
EEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure 
TKPKVLISDKMDSNAARIFEERGCDVDVITGESPEELRARIGEYEGLAIRSSTKVTPEI
CCCCEEEECCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCEEEECCCCCCHHH
LDAATNLKVIGRAGIGVDNVDIPYASSKGVVVMNTPFGNSITTAEHAVAMMFALARQIPQ
HHHHCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
ADRRTQAGEWPKNDFMGVELTGKILGLIGAGNIGSIVAARAQGLKMKVVAFDPFLTEERA
HHHHCCCCCCCCCCCEEEEEECEEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCC
IEIGVEKVDLDSLLARADFITLHTPLTDETRNILSQENLAKTKQGVRIINCARGGLIDEA
EEECCEEECHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
ALAEALDSGQVAGAALDVFQTEPAKDSPLFGKPNFICTPHLGASTTEAQVNVALQVAEQM
HHHHHHCCCCCCHHEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHH
ADYLVDGGVTNALNMPSLSAEEAPKLKPYMGLAENLGSLVGQLAHGNLTKISIEREGAAA
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
DLSGKPIEGAVLAGLMRQYSDTVNMVNAPFLAKERGLDIRSIRHEKEGAYNTLLRVTVGT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHCCCCCEEEEEEEECC
EQGDRSVAGTLFGSDAPRLVEIFGVRIEAQLEGHMLYIVNEDAPGFIGRIGTLLGEHGIN
CCCCCCEEEEECCCCCCCEEHHCCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHCCCE
IGTFNLGRRSAGGEAVLLLSVDQPIPQDVVKAACALDGVKTVMPLSF
EEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 7934829 [H]