Click here to switch to the map view.

The map label for this gene is murC

Identifier: 85373187

GI number: 85373187

Start: 404211

End: 405635

Strand: Direct

Name: murC

Synonym: ELI_01800

Alternate gene names: 85373187

Gene position: 404211-405635 (Clockwise)

Preceding gene: 85373186

Following gene: 85373188

Centisome position: 13.24

GC content: 64.98

Gene sequence:

>1425_bases
ATGAGGGGCGTTCCGACCGATATCGGGGTGATCCATTTCGTCGGCATCGGCGGCATCGGCATGTCCGGCATCGCCGAGGT
GATGCACAACCTCGGCTACCAGGTGCAGGGCAGCGACCTCGCCGAAGGGCCGAGCGTCGAGCGGCTGCGCGGGCGGGGCA
TCCAGGTGATGATCGGCCACGCGCCCGAGAATATCGAGAACGCGGCCGTGGTGGTGACGTCCACCGCGGTGAAGCGCACC
AATCCCGAAGTCGCCTCTGCGCTCGAAAACCGCATCCCGGTCGTGCGCCGGGCGGAAATGCTCGCCGAACTGATGCGATT
GAAGAGCACGGTCGCGGTGGCGGGCACCCACGGCAAGACGACGACCACCAGCATGATCGCGGCACTGCTCGATTGCGGCG
ATATCGATCCCACCGTCATAAACGGCGGCATCATCGAGCAATACGGCTCCAACGCGCGGCTCGGTGATAGCGAGTGGATG
GTGGTCGAAGCCGACGAAAGCGACGGCAGTTTCCTGCGGCTCGACGGCACGATTGCGGTCGTGACCAATATCGATCCCGA
GCACCTGGATCATTACGGCGATTTCGACGGCGTGAAGCGCGCCTTTCTCGACTTCATCCACAACGTGCCGTTCTATGGCG
CGGCGATCCTGTGCATCGACCATCCGGAAGTCCAGGCGGTGATCGGGCAGGTCCGCGACCGGCGCGTGATGACATACGGC
TTCTCGCTCCAGGCGGACATCTGCGGTGTCAACATCCGGCCCGAGGGCGGCGGCAACGTGTTCGACGTGATCGTGCGCCA
GCGGGGCGAGGAAGACCGTCGCATCGCCGATGTCCACCTGCCGATGCCGGGGCGGCATAATGTCCAGAATGCGCTCGCGG
CTATCGCGGTCTCGATCGAGATGGGTTGCAGCGATGAAGTAATCCAGCGCGGCTTCGAAAGCTTCGGCGGCGTCCGCCGG
CGCTTTACCAAGGTCGGCGAAGTCGGCGGTGCGACGATTATCGACGATTACGGCCATCACCCGGTGGAAATTGCCGCCGT
GCTCGGCGCGGCGCAGGAAGCGGTGTCTTCCAGCGATAAGGGCGGCCGCGTCATCGCGGTCGCGCAGCCGCATCGCTACA
CCCGCCTGCGCGATTTGATGGACGACTTCCAGAGTTGCTTCAACGATGCCGACGCGGTCTATGTCACCCCGGTCTATGAA
GCGGGTGAAGACCCGATCGACGGCATCGATGCAGAGGCGCTGGTCGCCGGATTGAAGTCGCGCGGACACCGCCATGCCGC
CACCATCGCGGACCCGCAGGCGCTCGCTAGAACGCTCGCCGGCGAGATCGAGGAAGGCGACGTGGTCGTCTGCCTTGGCG
CCGGGGATATCACCAAATGGGCTGCCGGCCTCGCCGATGCGATAGCGCAGGAGCGAGGCGCATGA

Upstream 100 bases:

>100_bases
GGGCGGACATGATGGATGTCATCAAGGTCGGCGGCAACAATGCCCGCGCCGCGTCGCAAGGCGTCGCCGTCGGGCAGGGT
GCGGCGAAGGAACGAGCCGA

Downstream 100 bases:

>100_bases
ACACGATCCAGCCAGACGACTGGGCGATGCACGATACCGGTGCCGCGCCGACGGCCGAAGTCGAAGGCGCGGTTGCCGCG
CCTGTGCCGATCGAGGGCAT

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: UDP-N-acetylmuramoyl-L-alanine synthetase

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRGVPTDIGVIHFVGIGGIGMSGIAEVMHNLGYQVQGSDLAEGPSVERLRGRGIQVMIGHAPENIENAAVVVTSTAVKRT
NPEVASALENRIPVVRRAEMLAELMRLKSTVAVAGTHGKTTTTSMIAALLDCGDIDPTVINGGIIEQYGSNARLGDSEWM
VVEADESDGSFLRLDGTIAVVTNIDPEHLDHYGDFDGVKRAFLDFIHNVPFYGAAILCIDHPEVQAVIGQVRDRRVMTYG
FSLQADICGVNIRPEGGGNVFDVIVRQRGEEDRRIADVHLPMPGRHNVQNALAAIAVSIEMGCSDEVIQRGFESFGGVRR
RFTKVGEVGGATIIDDYGHHPVEIAAVLGAAQEAVSSSDKGGRVIAVAQPHRYTRLRDLMDDFQSCFNDADAVYVTPVYE
AGEDPIDGIDAEALVAGLKSRGHRHAATIADPQALARTLAGEIEEGDVVVCLGAGDITKWAAGLADAIAQERGA

Sequences:

>Translated_474_residues
MRGVPTDIGVIHFVGIGGIGMSGIAEVMHNLGYQVQGSDLAEGPSVERLRGRGIQVMIGHAPENIENAAVVVTSTAVKRT
NPEVASALENRIPVVRRAEMLAELMRLKSTVAVAGTHGKTTTTSMIAALLDCGDIDPTVINGGIIEQYGSNARLGDSEWM
VVEADESDGSFLRLDGTIAVVTNIDPEHLDHYGDFDGVKRAFLDFIHNVPFYGAAILCIDHPEVQAVIGQVRDRRVMTYG
FSLQADICGVNIRPEGGGNVFDVIVRQRGEEDRRIADVHLPMPGRHNVQNALAAIAVSIEMGCSDEVIQRGFESFGGVRR
RFTKVGEVGGATIIDDYGHHPVEIAAVLGAAQEAVSSSDKGGRVIAVAQPHRYTRLRDLMDDFQSCFNDADAVYVTPVYE
AGEDPIDGIDAEALVAGLKSRGHRHAATIADPQALARTLAGEIEEGDVVVCLGAGDITKWAAGLADAIAQERGA
>Mature_474_residues
MRGVPTDIGVIHFVGIGGIGMSGIAEVMHNLGYQVQGSDLAEGPSVERLRGRGIQVMIGHAPENIENAAVVVTSTAVKRT
NPEVASALENRIPVVRRAEMLAELMRLKSTVAVAGTHGKTTTTSMIAALLDCGDIDPTVINGGIIEQYGSNARLGDSEWM
VVEADESDGSFLRLDGTIAVVTNIDPEHLDHYGDFDGVKRAFLDFIHNVPFYGAAILCIDHPEVQAVIGQVRDRRVMTYG
FSLQADICGVNIRPEGGGNVFDVIVRQRGEEDRRIADVHLPMPGRHNVQNALAAIAVSIEMGCSDEVIQRGFESFGGVRR
RFTKVGEVGGATIIDDYGHHPVEIAAVLGAAQEAVSSSDKGGRVIAVAQPHRYTRLRDLMDDFQSCFNDADAVYVTPVYE
AGEDPIDGIDAEALVAGLKSRGHRHAATIADPQALARTLAGEIEEGDVVVCLGAGDITKWAAGLADAIAQERGA

Specific function: Cell wall formation

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786279, Length=466, Percent_Identity=44.8497854077253, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI1790680, Length=396, Percent_Identity=28.7878787878788, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURC_ERYLH (Q2NCY9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457249.1
- ProteinModelPortal:   Q2NCY9
- SMR:   Q2NCY9
- STRING:   Q2NCY9
- GeneID:   3868845
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01800
- NMPDR:   fig|314225.3.peg.2729
- eggNOG:   COG0773
- HOGENOM:   HBG476594
- OMA:   YQPHRYS
- PhylomeDB:   Q2NCY9
- ProtClustDB:   PRK00421
- BioCyc:   ELIT314225:ELI_01800-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00046
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR016040
- InterPro:   IPR005758
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01082

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.8

Molecular weight: Translated: 50502; Mature: 50502

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00318 HMG_COA_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGVPTDIGVIHFVGIGGIGMSGIAEVMHNLGYQVQGSDLAEGPSVERLRGRGIQVMIGH
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCCHHHHCCCCEEEEECC
APENIENAAVVVTSTAVKRTNPEVASALENRIPVVRRAEMLAELMRLKSTVAVAGTHGKT
CCCCCCCCEEEEEECHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCH
TTTSMIAALLDCGDIDPTVINGGIIEQYGSNARLGDSEWMVVEADESDGSFLRLDGTIAV
HHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCCCCCCEEEEEECCCCCCEEEECCEEEE
VTNIDPEHLDHYGDFDGVKRAFLDFIHNVPFYGAAILCIDHPEVQAVIGQVRDRRVMTYG
EECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCEEEEEC
FSLQADICGVNIRPEGGGNVFDVIVRQRGEEDRRIADVHLPMPGRHNVQNALAAIAVSIE
CEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHEEEE
MGCSDEVIQRGFESFGGVRRRFTKVGEVGGATIIDDYGHHPVEIAAVLGAAQEAVSSSDK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
GGRVIAVAQPHRYTRLRDLMDDFQSCFNDADAVYVTPVYEAGEDPIDGIDAEALVAGLKS
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCCCCCCCCCHHHHHHHHHH
RGHRHAATIADPQALARTLAGEIEEGDVVVCLGAGDITKWAAGLADAIAQERGA
CCCCCEECCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGVPTDIGVIHFVGIGGIGMSGIAEVMHNLGYQVQGSDLAEGPSVERLRGRGIQVMIGH
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCCHHHHCCCCEEEEECC
APENIENAAVVVTSTAVKRTNPEVASALENRIPVVRRAEMLAELMRLKSTVAVAGTHGKT
CCCCCCCCEEEEEECHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCCH
TTTSMIAALLDCGDIDPTVINGGIIEQYGSNARLGDSEWMVVEADESDGSFLRLDGTIAV
HHHHHHHHHHHCCCCCCEEECCCHHHHCCCCCCCCCCCEEEEEECCCCCCEEEECCEEEE
VTNIDPEHLDHYGDFDGVKRAFLDFIHNVPFYGAAILCIDHPEVQAVIGQVRDRRVMTYG
EECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCEEEEEC
FSLQADICGVNIRPEGGGNVFDVIVRQRGEEDRRIADVHLPMPGRHNVQNALAAIAVSIE
CEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHEEEE
MGCSDEVIQRGFESFGGVRRRFTKVGEVGGATIIDDYGHHPVEIAAVLGAAQEAVSSSDK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
GGRVIAVAQPHRYTRLRDLMDDFQSCFNDADAVYVTPVYEAGEDPIDGIDAEALVAGLKS
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCCCCCCCCCHHHHHHHHHH
RGHRHAATIADPQALARTLAGEIEEGDVVVCLGAGDITKWAAGLADAIAQERGA
CCCCCEECCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA