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The map label for this gene is murE

Identifier: 85373181

GI number: 85373181

Start: 396388

End: 397875

Strand: Direct

Name: murE

Synonym: ELI_01770

Alternate gene names: 85373181

Gene position: 396388-397875 (Clockwise)

Preceding gene: 85373180

Following gene: 85373182

Centisome position: 12.99

GC content: 66.06

Gene sequence:

>1488_bases
GTGAAGCTTGCCCGGCTGGCAGAAGCGGCAGGCATTGCCATCGAAGGCGGCGAGGACATCGCCGTCTCGGGTTTTGCCAT
CGACCATCGCAAGGTCGCGCCGGGCACCGTCTTCGGTGCCTTTCAGGGCTCCGCCGTCAACGGAGAGGACTATATTTCGC
AGGCCGTCGCCGCCGGGGCGGTCGCCGTGGTAGCGCAGCCCGAGGCCACGGTCGAAGGCGCGGTCCACATCGCCGAGGAT
ACGCCGCGCCGCGCCTTTGCCCTGCTGGCGGCGCAGTATTTCACCCCGGTGCCGAAAACGATCGTCGCGGTCACAGGGAC
CAATGGCAAGACCTCGACCGTCGAGATGACCCGCCAGCTTTGGCGCATGGCGGGCGAGCGCGCCGCCAGTATCGGCACGC
TTGGCGTGACGACGCCGGACGAGAGTGTTTCGACCGGTTTGACGACGCCCGACATCGTCACTTTCCTCAGCAATATGAGC
GGGCTCGCGCGCGAAGGCGTGACCCATGTTGCCTACGAGGCTTCGAGCCACGGCCTTTCGCAATATCGCAACGAGGGTCT
GCCCGTCGCAGCAGCGGCCTTCACCAATTTCAGCCGCGATCATCTCGATTATCACGGCACGATGGACGAATATTTCGAGG
CCAAGATGCGCCTGTTCGACGAAGTCGTCGATGCAGGCGGGACGGCCGTGGTCTGGACGGGCAATAGCGAGTGGACCGCC
CAGGCCATCGAGCGGGCGCGCCAGCGAGAACTGCGCCTGTTCACGGTGGGCGAGCAGGGTGAGGACATCCGCCTGCGCGC
CCGTACCCCCACCCAATTGGGGCAGGAACTCGAGATCGAACATGCTGGCGAGACGCGCAAGCTGCGGCTGCCGCTGATCG
GTGAGTACCAGGTCGCCAACGCGCTGACCGCCGCCGGCCTCGCCATCGCAACGGGCACCGACCCCGCCGCAGTCTGGGAT
GGCGTCAGTCGGCTGCAGCCGGTGCGCGGACGCTTGGAGCGCGCGGTCATCACGGCAACAGGCGCGCCTGTCTACGTCGA
CTATGCTCACACACCTGACGCGCTCGAAGCGGCGCTGGCGGCGCTGCGCCCGCATGTCGAAGGGAGGCTGCTGTGCATTT
TCGGTGCCGGCGGCGACCGCGATACAGGCAAGCGCGCGCCGATGGGCGAAGTCGCTTCGCGCATGGCGGATGCCGTGATC
GTCACCGACGACAATCCGCGCGGCGAAGACCCTGCGGCGATCCGTGGGCAGGTGCTTGAAGGCGCAGGCGACAATGCGCG
AGAAATGGCGGACCGGCGCGAGGCTATCCGCGAGGTCATCGAATCAGCCGCGGCGAAGGACATCGTCCTGATCGCCGGCA
AGGGACACGAAACCGGACAAATCATCGGGTCGGGAGAGAATATGCGGGTCGAACCGTTTGACGACGTACAGGTCGCACGC
GAATGCGCGGCGCAGATTTCGAAAAATGCCTGGGGCGCAACCGAATGA

Upstream 100 bases:

>100_bases
AGCTGGTGCCCCGCATCGGCCCGATCCTCGGCGTGCGCCCCGACATGACCCGCGACGTCGATATCTCCGACCTCAAGCGC
CTTGTGCCGGGAGCGGCCGA

Downstream 100 bases:

>100_bases
GCATGCACAGTGCCTTGCTGAAATGGCCGGTCACGCGGCGCGACGCGCTGCCCGTGGCGCTCTGGTCCGCGCAGGAGATT
GCTGCGGCGACCGGTGGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKLARLAEAAGIAIEGGEDIAVSGFAIDHRKVAPGTVFGAFQGSAVNGEDYISQAVAAGAVAVVAQPEATVEGAVHIAED
TPRRAFALLAAQYFTPVPKTIVAVTGTNGKTSTVEMTRQLWRMAGERAASIGTLGVTTPDESVSTGLTTPDIVTFLSNMS
GLAREGVTHVAYEASSHGLSQYRNEGLPVAAAAFTNFSRDHLDYHGTMDEYFEAKMRLFDEVVDAGGTAVVWTGNSEWTA
QAIERARQRELRLFTVGEQGEDIRLRARTPTQLGQELEIEHAGETRKLRLPLIGEYQVANALTAAGLAIATGTDPAAVWD
GVSRLQPVRGRLERAVITATGAPVYVDYAHTPDALEAALAALRPHVEGRLLCIFGAGGDRDTGKRAPMGEVASRMADAVI
VTDDNPRGEDPAAIRGQVLEGAGDNAREMADRREAIREVIESAAAKDIVLIAGKGHETGQIIGSGENMRVEPFDDVQVAR
ECAAQISKNAWGATE

Sequences:

>Translated_495_residues
MKLARLAEAAGIAIEGGEDIAVSGFAIDHRKVAPGTVFGAFQGSAVNGEDYISQAVAAGAVAVVAQPEATVEGAVHIAED
TPRRAFALLAAQYFTPVPKTIVAVTGTNGKTSTVEMTRQLWRMAGERAASIGTLGVTTPDESVSTGLTTPDIVTFLSNMS
GLAREGVTHVAYEASSHGLSQYRNEGLPVAAAAFTNFSRDHLDYHGTMDEYFEAKMRLFDEVVDAGGTAVVWTGNSEWTA
QAIERARQRELRLFTVGEQGEDIRLRARTPTQLGQELEIEHAGETRKLRLPLIGEYQVANALTAAGLAIATGTDPAAVWD
GVSRLQPVRGRLERAVITATGAPVYVDYAHTPDALEAALAALRPHVEGRLLCIFGAGGDRDTGKRAPMGEVASRMADAVI
VTDDNPRGEDPAAIRGQVLEGAGDNAREMADRREAIREVIESAAAKDIVLIAGKGHETGQIIGSGENMRVEPFDDVQVAR
ECAAQISKNAWGATE
>Mature_495_residues
MKLARLAEAAGIAIEGGEDIAVSGFAIDHRKVAPGTVFGAFQGSAVNGEDYISQAVAAGAVAVVAQPEATVEGAVHIAED
TPRRAFALLAAQYFTPVPKTIVAVTGTNGKTSTVEMTRQLWRMAGERAASIGTLGVTTPDESVSTGLTTPDIVTFLSNMS
GLAREGVTHVAYEASSHGLSQYRNEGLPVAAAAFTNFSRDHLDYHGTMDEYFEAKMRLFDEVVDAGGTAVVWTGNSEWTA
QAIERARQRELRLFTVGEQGEDIRLRARTPTQLGQELEIEHAGETRKLRLPLIGEYQVANALTAAGLAIATGTDPAAVWD
GVSRLQPVRGRLERAVITATGAPVYVDYAHTPDALEAALAALRPHVEGRLLCIFGAGGDRDTGKRAPMGEVASRMADAVI
VTDDNPRGEDPAAIRGQVLEGAGDNAREMADRREAIREVIESAAAKDIVLIAGKGHETGQIIGSGENMRVEPFDDVQVAR
ECAAQISKNAWGATE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=477, Percent_Identity=38.5744234800839, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1786274, Length=323, Percent_Identity=28.7925696594427, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52438; Mature: 52438

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLARLAEAAGIAIEGGEDIAVSGFAIDHRKVAPGTVFGAFQGSAVNGEDYISQAVAAGA
CCHHHHHHHCCEEEECCCCEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCE
VAVVAQPEATVEGAVHIAEDTPRRAFALLAAQYFTPVPKTIVAVTGTNGKTSTVEMTRQL
EEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
WRMAGERAASIGTLGVTTPDESVSTGLTTPDIVTFLSNMSGLAREGVTHVAYEASSHGLS
HHHHHHHHHCCCEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHH
QYRNEGLPVAAAAFTNFSRDHLDYHGTMDEYFEAKMRLFDEVVDAGGTAVVWTGNSEWTA
HHHCCCCCEEEHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHH
QAIERARQRELRLFTVGEQGEDIRLRARTPTQLGQELEIEHAGETRKLRLPLIGEYQVAN
HHHHHHHHCCEEEEEECCCCCCEEEEECCHHHCCCEEEEEECCCCEEEECCCCCCHHHHH
ALTAAGLAIATGTDPAAVWDGVSRLQPVRGRLERAVITATGAPVYVDYAHTPDALEAALA
HHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCHHHHHHHH
ALRPHVEGRLLCIFGAGGDRDTGKRAPMGEVASRMADAVIVTDDNPRGEDPAAIRGQVLE
HHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHCC
GAGDNAREMADRREAIREVIESAAAKDIVLIAGKGHETGQIIGSGENMRVEPFDDVQVAR
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCEECCCHHHHHHH
ECAAQISKNAWGATE
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLARLAEAAGIAIEGGEDIAVSGFAIDHRKVAPGTVFGAFQGSAVNGEDYISQAVAAGA
CCHHHHHHHCCEEEECCCCEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCE
VAVVAQPEATVEGAVHIAEDTPRRAFALLAAQYFTPVPKTIVAVTGTNGKTSTVEMTRQL
EEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHH
WRMAGERAASIGTLGVTTPDESVSTGLTTPDIVTFLSNMSGLAREGVTHVAYEASSHGLS
HHHHHHHHHCCCEEECCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHH
QYRNEGLPVAAAAFTNFSRDHLDYHGTMDEYFEAKMRLFDEVVDAGGTAVVWTGNSEWTA
HHHCCCCCEEEHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHH
QAIERARQRELRLFTVGEQGEDIRLRARTPTQLGQELEIEHAGETRKLRLPLIGEYQVAN
HHHHHHHHCCEEEEEECCCCCCEEEEECCHHHCCCEEEEEECCCCEEEECCCCCCHHHHH
ALTAAGLAIATGTDPAAVWDGVSRLQPVRGRLERAVITATGAPVYVDYAHTPDALEAALA
HHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCCHHHHHHHH
ALRPHVEGRLLCIFGAGGDRDTGKRAPMGEVASRMADAVIVTDDNPRGEDPAAIRGQVLE
HHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHCC
GAGDNAREMADRREAIREVIESAAAKDIVLIAGKGHETGQIIGSGENMRVEPFDDVQVAR
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCEECCCHHHHHHH
ECAAQISKNAWGATE
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA