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The map label for this gene is pyrB

Identifier: 85373033

GI number: 85373033

Start: 243577

End: 244620

Strand: Direct

Name: pyrB

Synonym: ELI_01030

Alternate gene names: 85373033

Gene position: 243577-244620 (Clockwise)

Preceding gene: 85373027

Following gene: 85373034

Centisome position: 7.98

GC content: 64.56

Gene sequence:

>1044_bases
ATGACAAGCGACGGCACACCCACGCAATCCGGCCGCTATCCTGCGGGCGGGCACGCGTTTCCGCACGGCAGCCTGACCGG
TATCGCCCAGCTCGAACGGCATGAGATCCTCTACCTGCTCGACGAGGCTGAGCAGTGGGTCGATTTCAACAGGCAGAGCG
AAAAACACTGCGATTTGCTGGCCGGCCTTACGATCATCAACGCCTTCTTCGAAAACAGCACGCGCACGCTGCTTAGCTTC
GAGATTGCGGGGAAGCGGCTGGGGGCGGATGTCGTCAACATGCATGCGGCGCAGTCTTCGGTGAAGAAAGGCGAGACGCT
GATCGATACCGCGATCACGCTCAATGCCATGCGCGCCGACGCTATCGTCATCCGGCACGGCTCCAGCGGGGCGGTGGACC
TGATTGCGAGCAAGGTCGATTGCCCCGTGCTCAATGCCGGCGACGGCCAGCACGAACATCCGACGCAGGCGCTGCTCGAT
GCGCTGGCGTTGCGGCATGCACTGGAAGCGCGGGGCGAGGGGTCGGAGGATTTTACCGGCCTCACGATCACCATCTGCGG
CGATATCCTGCACAGCCGCGTCGCGCGCTCCAACATCCTGTGCCTTCAGGCGATGGGGGCGAGCGTGCGGGTCTGCGCGC
CGCCGGCGCTGATGCCGGTCGGGATCGAGCGGATGGGGGCGGAGCCGTTCCACGATTTCGATGCCGCGCTCAAGGGCTGC
GATGTCGCGATGATGTTGCGTTTGCAGACCGAACGCATGAGCGGGCAATTTATCCCCTCGGCGCGCGAGTATCACCATTT
GTACGGGCTCACGCAGCACCGTTTGCAGCGCTGTGCCCCCGATGCGCTGGTCATGCACCCCGGGCCGATGAATCGCGGGG
TCGAGATCGACAGCGATGTCGCCGACATGCTCGATCGCTCGATCATTACGCGTCAGGTCGAGATGGGCGTCGCCGTACGC
ATGGCTTGCCTGGACATCTTGACGCGCAAGGGGCGCGGGCTGCCCGGCTGGCAGGGCGACCTCGGCAAGGGGCAATGGGC
ATGA

Upstream 100 bases:

>100_bases
ACCCTTGTATTGCCTGGCTGACACATAGGCAGCCAAACCGCGCATCGCCAGCCGAAACCGCTTGAGCGCTCAACATGGCT
CGACTAAAGGCTGCGCTTTA

Downstream 100 bases:

>100_bases
AGCAGCAGCGCCCCCTTCTGATTACGAACGGCAAGGTCGTCACCCCCGACGGGGTCCGCGAGCAGCCGATAGTTTGCATC
GATGGCCGCATCGCCGCCGT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTSDGTPTQSGRYPAGGHAFPHGSLTGIAQLERHEILYLLDEAEQWVDFNRQSEKHCDLLAGLTIINAFFENSTRTLLSF
EIAGKRLGADVVNMHAAQSSVKKGETLIDTAITLNAMRADAIVIRHGSSGAVDLIASKVDCPVLNAGDGQHEHPTQALLD
ALALRHALEARGEGSEDFTGLTITICGDILHSRVARSNILCLQAMGASVRVCAPPALMPVGIERMGAEPFHDFDAALKGC
DVAMMLRLQTERMSGQFIPSAREYHHLYGLTQHRLQRCAPDALVMHPGPMNRGVEIDSDVADMLDRSIITRQVEMGVAVR
MACLDILTRKGRGLPGWQGDLGKGQWA

Sequences:

>Translated_347_residues
MTSDGTPTQSGRYPAGGHAFPHGSLTGIAQLERHEILYLLDEAEQWVDFNRQSEKHCDLLAGLTIINAFFENSTRTLLSF
EIAGKRLGADVVNMHAAQSSVKKGETLIDTAITLNAMRADAIVIRHGSSGAVDLIASKVDCPVLNAGDGQHEHPTQALLD
ALALRHALEARGEGSEDFTGLTITICGDILHSRVARSNILCLQAMGASVRVCAPPALMPVGIERMGAEPFHDFDAALKGC
DVAMMLRLQTERMSGQFIPSAREYHHLYGLTQHRLQRCAPDALVMHPGPMNRGVEIDSDVADMLDRSIITRQVEMGVAVR
MACLDILTRKGRGLPGWQGDLGKGQWA
>Mature_346_residues
TSDGTPTQSGRYPAGGHAFPHGSLTGIAQLERHEILYLLDEAEQWVDFNRQSEKHCDLLAGLTIINAFFENSTRTLLSFE
IAGKRLGADVVNMHAAQSSVKKGETLIDTAITLNAMRADAIVIRHGSSGAVDLIASKVDCPVLNAGDGQHEHPTQALLDA
LALRHALEARGEGSEDFTGLTITICGDILHSRVARSNILCLQAMGASVRVCAPPALMPVGIERMGAEPFHDFDAALKGCD
VAMMLRLQTERMSGQFIPSAREYHHLYGLTQHRLQRCAPDALVMHPGPMNRGVEIDSDVADMLDRSIITRQVEMGVAVRM
ACLDILTRKGRGLPGWQGDLGKGQWA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=285, Percent_Identity=40, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI2367364, Length=287, Percent_Identity=35.191637630662, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI193204318, Length=347, Percent_Identity=30.835734870317, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322331, Length=280, Percent_Identity=37.1428571428571, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24642586, Length=289, Percent_Identity=35.9861591695502, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_ERYLH (Q2NDE3)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457095.1
- HSSP:   P96108
- ProteinModelPortal:   Q2NDE3
- SMR:   Q2NDE3
- STRING:   Q2NDE3
- GeneID:   3871335
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01030
- NMPDR:   fig|314225.3.peg.2882
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   ELIT314225:ELI_01030-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37605; Mature: 37473

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDGTPTQSGRYPAGGHAFPHGSLTGIAQLERHEILYLLDEAEQWVDFNRQSEKHCDLL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEHHHHHHHCCCCCCHHHHHHH
AGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVKKGETLIDTAITLNAMRAD
HHHHHHHHHHCCCCCEEEEEEECCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECC
AIVIRHGSSGAVDLIASKVDCPVLNAGDGQHEHPTQALLDALALRHALEARGEGSEDFTG
EEEEEECCCCCHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
LTITICGDILHSRVARSNILCLQAMGASVRVCAPPALMPVGIERMGAEPFHDFDAALKGC
EEEEHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCCCCCCHHHHCCCCCHHHHHHHCCC
DVAMMLRLQTERMSGQFIPSAREYHHLYGLTQHRLQRCAPDALVMHPGPMNRGVEIDSDV
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHH
ADMLDRSIITRQVEMGVAVRMACLDILTRKGRGLPGWQGDLGKGQWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSDGTPTQSGRYPAGGHAFPHGSLTGIAQLERHEILYLLDEAEQWVDFNRQSEKHCDLL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEHHHHHHHCCCCCCHHHHHHH
AGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVKKGETLIDTAITLNAMRAD
HHHHHHHHHHCCCCCEEEEEEECCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECC
AIVIRHGSSGAVDLIASKVDCPVLNAGDGQHEHPTQALLDALALRHALEARGEGSEDFTG
EEEEEECCCCCHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
LTITICGDILHSRVARSNILCLQAMGASVRVCAPPALMPVGIERMGAEPFHDFDAALKGC
EEEEHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCCCCCCHHHHCCCCCHHHHHHHCCC
DVAMMLRLQTERMSGQFIPSAREYHHLYGLTQHRLQRCAPDALVMHPGPMNRGVEIDSDV
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHH
ADMLDRSIITRQVEMGVAVRMACLDILTRKGRGLPGWQGDLGKGQWA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA