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The map label for this gene is btuB [H]

Identifier: 85373018

GI number: 85373018

Start: 224349

End: 226664

Strand: Direct

Name: btuB [H]

Synonym: ELI_00955

Alternate gene names: 85373018

Gene position: 224349-226664 (Clockwise)

Preceding gene: 85373015

Following gene: 85373019

Centisome position: 7.35

GC content: 64.21

Gene sequence:

>2316_bases
ATGAATCTCGCGCTTCTCGCTTTAACCATAACCGCCGGCGCTATCGTCACCTCCCCTCTGCAAGCTCAGGATTTGCCTTC
CGAAGCGGAGGGCCCGGTCGTGAAGGACCCCGCCAATACCATCGTGGTGACCGGCACCCGCAGCGCCGGACGTAGCGCAC
TGGAGGCAGCTGTACCGGTGGACGTTGTCAGCGAAGAGGAGCTGAACGCCACCGGGTATGCCGATCTCAACCGCGCGCTG
AATTTCCTCGAGCCGTCGGTCAATTTTCCGCGCGCGGCCACCACCGCCACGGCGGCGAACACGCGGCCGATCACGCTACG
CGGCCTGTCGCCCGATCAGACGCTCGTGCTCGTTAACGGCAAGCGCCGCCACACCAACGCCGTGCTCAATGTAAACAATT
CGATCGGGCGCGGTTCGGCGGGCGTCGATCTCGACACGATCCCTCAGAACGCGATCCAGCGGATCGAGATCCTGCGCGAC
GGCGCGGCAGCGCAGTATGGCTCGGACGCGATCGGCGGGGTAGTGAACATCATCCTGAAGGACGATGCCCAGGGCGCTTC
GTTCGAACTGCTGGGTGGCATCACCGAGGAAGGCGACGGGCTCAACGGGCTCGCCTCGACCAGCGGCGGGCTGCCGATCG
GTCGGGGCGGTCATTTGACTCTCAGTGCATTGGTCCGCGCGCAGGAAGCGACCAACCGCGCCTTTGTCGATCAGCGCTTC
GACCGGGTAACCTACCGGATAGGCGATCCGGAAGCCTCGCTTTTTGGCGGATCGGTCAATTTCGGCCTGCCACTCGGCGA
CGCGGAACTCTACGGTTTCGGCACGGTCGCGCAAAAAATTTCGAACAACGGTGCGGGCTTTCGCGTGCCCGGCACCTCGC
TGCTCTATCCCGACGGTTTCCTGCCGGTCATCGAACCGTACATCTGGGATGGCAGCGGGACCTTGGGTATCTCCGCCGAT
CTCGGCGGCTTCGCGCTCGATCTGAGCCAGACCTACGGCCGCAACGAGGCGGATTTCCGCGTCTTCGATACGGTGAATGT
GTCGCTGGGTCTCGACAGCCCGACCAGCTTTGATTCGGGCGGCGTGACCTATGAACAATATGTCACCGATTTCGGCGTCT
CGCGCTCGCTCGGACTTCTCGCCGGAGGCAATATCACCGCCGGCGCGCAGTACCGCCGCGAAACCTACGCCATCCGCGAG
GGAGAACCCGCCGCTTCGACCGGAGTCGGTGCGGACGGTTTCGCGGGGTTCGCTCCGCGCCGACCGGTGAATGAGGTGCG
CGATGCCTTTGCATTCTATCTCGACGCCGAGCTGCGCCCGATCGAGGCGCTGCTGATCGGCGGGGCGGTCCGTTACGACG
AGTATGACGATTCCGGTGGAGAGACGACCTGGCGGGTTTCAGCCCGCGCCGATCTGACAGAGGGCCTGGCGCTGCGCGGC
GCGATCGGAACTGCGTTCCGCGCGCCTTCGTTGCAACAGCAGAATTTCAGCGCGGTGCAGGGCGCACTTTCGGCAGGGCA
GCTGGTTTCGGTAGCGACGCTGCCGGTGAGCGACCCGATCGCGCGCGCGTTGGGTGCGGGAGATCTGCGACCCGAGCGCT
CACGTAATTACAGCGCGGGTATCGTGCTGGAGCCGACGCCCCGCCTCGCTTTCACCTTGGATGCGTTTCACATCCAAATC
GATGATCGCATCGCGCTGAGTGAACAGCTGCGCGGGGCGGCGGTGAATGCCATTCTGGCCGGCGCTGGTATAGTTGGCTT
CTCGCAGGTACGCTTCTTCACCAACGCGGTCGATACCAGGACTACCGGTGTGGAGGCCACCGCGCGCTGGTCGGCCAGCC
TCAGTCCGGAAACGCGGGCAAGCATGGATGTCGGCTTCGCGCTCTATGACACGGAATTGGAAAGTCTCCGCGCCAATCCG
GTGCTGCCGACTCTGCCGTTGCTCGGCGAGAAGTCGATCTTGTTCCTGACCGAAGCGCAGCCGCGCGACAAGCTAACCGT
GCAGGCTGTCGTCGAGCACCGGTCAGTGAGCGCACGTGCAGCGCTGACCCGTTTCGGCACCTACACCTCGCAACCGCTCG
TGGAGACGCAGACCTTCGATGGCGGCACGTCATTCGACCTGTCGCTGAGCTACGCCCTGTCTTCCGCGATCATGCTTACC
GCCGGCGTGCAGAACCTGTTCGACGACCGGCCGGAAGAGATTGCTGATCAGGCGCGCTTCATCGCCGCAACCGGCGGCAG
CTTTCCCACGGGCGAGGAGACGCCGCTCGGCGTCAACGGGCGTAGCTATTTCGTGCAGCTACGAGGGAATTTCTGA

Upstream 100 bases:

>100_bases
GACAAGCAACACCGTGCCGGTCTGCGCAATACCCGGCCCAGCCAAGCCTTCGTTACATGCGGCCCCGTTCGCAGTTGCAG
CAAATCGGATCGGGCCAACG

Downstream 100 bases:

>100_bases
TGGTGTCGCGTCGCGCATTGCTTGGCCGGACGGCGCTGGCGCTGGCTGCGTCTCGCTTCCTGTCTCCGGTAACGGCCATC
GCTGTGCAACCGCAACCATA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 771; Mature: 771

Protein sequence:

>771_residues
MNLALLALTITAGAIVTSPLQAQDLPSEAEGPVVKDPANTIVVTGTRSAGRSALEAAVPVDVVSEEELNATGYADLNRAL
NFLEPSVNFPRAATTATAANTRPITLRGLSPDQTLVLVNGKRRHTNAVLNVNNSIGRGSAGVDLDTIPQNAIQRIEILRD
GAAAQYGSDAIGGVVNIILKDDAQGASFELLGGITEEGDGLNGLASTSGGLPIGRGGHLTLSALVRAQEATNRAFVDQRF
DRVTYRIGDPEASLFGGSVNFGLPLGDAELYGFGTVAQKISNNGAGFRVPGTSLLYPDGFLPVIEPYIWDGSGTLGISAD
LGGFALDLSQTYGRNEADFRVFDTVNVSLGLDSPTSFDSGGVTYEQYVTDFGVSRSLGLLAGGNITAGAQYRRETYAIRE
GEPAASTGVGADGFAGFAPRRPVNEVRDAFAFYLDAELRPIEALLIGGAVRYDEYDDSGGETTWRVSARADLTEGLALRG
AIGTAFRAPSLQQQNFSAVQGALSAGQLVSVATLPVSDPIARALGAGDLRPERSRNYSAGIVLEPTPRLAFTLDAFHIQI
DDRIALSEQLRGAAVNAILAGAGIVGFSQVRFFTNAVDTRTTGVEATARWSASLSPETRASMDVGFALYDTELESLRANP
VLPTLPLLGEKSILFLTEAQPRDKLTVQAVVEHRSVSARAALTRFGTYTSQPLVETQTFDGGTSFDLSLSYALSSAIMLT
AGVQNLFDDRPEEIADQARFIAATGGSFPTGEETPLGVNGRSYFVQLRGNF

Sequences:

>Translated_771_residues
MNLALLALTITAGAIVTSPLQAQDLPSEAEGPVVKDPANTIVVTGTRSAGRSALEAAVPVDVVSEEELNATGYADLNRAL
NFLEPSVNFPRAATTATAANTRPITLRGLSPDQTLVLVNGKRRHTNAVLNVNNSIGRGSAGVDLDTIPQNAIQRIEILRD
GAAAQYGSDAIGGVVNIILKDDAQGASFELLGGITEEGDGLNGLASTSGGLPIGRGGHLTLSALVRAQEATNRAFVDQRF
DRVTYRIGDPEASLFGGSVNFGLPLGDAELYGFGTVAQKISNNGAGFRVPGTSLLYPDGFLPVIEPYIWDGSGTLGISAD
LGGFALDLSQTYGRNEADFRVFDTVNVSLGLDSPTSFDSGGVTYEQYVTDFGVSRSLGLLAGGNITAGAQYRRETYAIRE
GEPAASTGVGADGFAGFAPRRPVNEVRDAFAFYLDAELRPIEALLIGGAVRYDEYDDSGGETTWRVSARADLTEGLALRG
AIGTAFRAPSLQQQNFSAVQGALSAGQLVSVATLPVSDPIARALGAGDLRPERSRNYSAGIVLEPTPRLAFTLDAFHIQI
DDRIALSEQLRGAAVNAILAGAGIVGFSQVRFFTNAVDTRTTGVEATARWSASLSPETRASMDVGFALYDTELESLRANP
VLPTLPLLGEKSILFLTEAQPRDKLTVQAVVEHRSVSARAALTRFGTYTSQPLVETQTFDGGTSFDLSLSYALSSAIMLT
AGVQNLFDDRPEEIADQARFIAATGGSFPTGEETPLGVNGRSYFVQLRGNF
>Mature_771_residues
MNLALLALTITAGAIVTSPLQAQDLPSEAEGPVVKDPANTIVVTGTRSAGRSALEAAVPVDVVSEEELNATGYADLNRAL
NFLEPSVNFPRAATTATAANTRPITLRGLSPDQTLVLVNGKRRHTNAVLNVNNSIGRGSAGVDLDTIPQNAIQRIEILRD
GAAAQYGSDAIGGVVNIILKDDAQGASFELLGGITEEGDGLNGLASTSGGLPIGRGGHLTLSALVRAQEATNRAFVDQRF
DRVTYRIGDPEASLFGGSVNFGLPLGDAELYGFGTVAQKISNNGAGFRVPGTSLLYPDGFLPVIEPYIWDGSGTLGISAD
LGGFALDLSQTYGRNEADFRVFDTVNVSLGLDSPTSFDSGGVTYEQYVTDFGVSRSLGLLAGGNITAGAQYRRETYAIRE
GEPAASTGVGADGFAGFAPRRPVNEVRDAFAFYLDAELRPIEALLIGGAVRYDEYDDSGGETTWRVSARADLTEGLALRG
AIGTAFRAPSLQQQNFSAVQGALSAGQLVSVATLPVSDPIARALGAGDLRPERSRNYSAGIVLEPTPRLAFTLDAFHIQI
DDRIALSEQLRGAAVNAILAGAGIVGFSQVRFFTNAVDTRTTGVEATARWSASLSPETRASMDVGFALYDTELESLRANP
VLPTLPLLGEKSILFLTEAQPRDKLTVQAVVEHRSVSARAALTRFGTYTSQPLVETQTFDGGTSFDLSLSYALSSAIMLT
AGVQNLFDDRPEEIADQARFIAATGGSFPTGEETPLGVNGRSYFVQLRGNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=202, Percent_Identity=32.6732673267327, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1786798, Length=186, Percent_Identity=33.8709677419355, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 81161; Mature: 81161

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLALLALTITAGAIVTSPLQAQDLPSEAEGPVVKDPANTIVVTGTRSAGRSALEAAVPV
CCEEEEEEEECCCHHEECCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHCCE
DVVSEEELNATGYADLNRALNFLEPSVNFPRAATTATAANTRPITLRGLSPDQTLVLVNG
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEEECCCCCCEEEEECC
KRRHTNAVLNVNNSIGRGSAGVDLDTIPQNAIQRIEILRDGAAAQYGSDAIGGVVNIILK
CCCCCCEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCEEEEEEE
DDAQGASFELLGGITEEGDGLNGLASTSGGLPIGRGGHLTLSALVRAQEATNRAFVDQRF
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
DRVTYRIGDPEASLFGGSVNFGLPLGDAELYGFGTVAQKISNNGAGFRVPGTSLLYPDGF
CEEEEEECCCCCEEECCCEECCCCCCCCCEEEHHHHHHHHCCCCCEEECCCCEEECCCCC
LPVIEPYIWDGSGTLGISADLGGFALDLSQTYGRNEADFRVFDTVNVSLGLDSPTSFDSG
HHHCCCEEECCCCCEEEEECCCCEEEEEHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCC
GVTYEQYVTDFGVSRSLGLLAGGNITAGAQYRRETYAIREGEPAASTGVGADGFAGFAPR
CEEHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCC
RPVNEVRDAFAFYLDAELRPIEALLIGGAVRYDEYDDSGGETTWRVSARADLTEGLALRG
CCHHHHHHHHEEEECCCCCCEEEEEECCEEEECCCCCCCCCEEEEEEECCCCCCCEEEEC
AIGTAFRAPSLQQQNFSAVQGALSAGQLVSVATLPVSDPIARALGAGDLRPERSRNYSAG
CCCHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCC
IVLEPTPRLAFTLDAFHIQIDDRIALSEQLRGAAVNAILAGAGIVGFSQVRFFTNAVDTR
EEECCCCCEEEEEEEEEEEECCCCHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
TTGVEATARWSASLSPETRASMDVGFALYDTELESLRANPVLPTLPLLGEKSILFLTEAQ
CCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCEEEEEECCC
PRDKLTVQAVVEHRSVSARAALTRFGTYTSQPLVETQTFDGGTSFDLSLSYALSSAIMLT
CCCCEEHEEHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHH
AGVQNLFDDRPEEIADQARFIAATGGSFPTGEETPLGVNGRSYFVQLRGNF
HHHHHHHCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNLALLALTITAGAIVTSPLQAQDLPSEAEGPVVKDPANTIVVTGTRSAGRSALEAAVPV
CCEEEEEEEECCCHHEECCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHCCE
DVVSEEELNATGYADLNRALNFLEPSVNFPRAATTATAANTRPITLRGLSPDQTLVLVNG
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEEECCCCCCEEEEECC
KRRHTNAVLNVNNSIGRGSAGVDLDTIPQNAIQRIEILRDGAAAQYGSDAIGGVVNIILK
CCCCCCEEEECCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCEEEEEEE
DDAQGASFELLGGITEEGDGLNGLASTSGGLPIGRGGHLTLSALVRAQEATNRAFVDQRF
CCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
DRVTYRIGDPEASLFGGSVNFGLPLGDAELYGFGTVAQKISNNGAGFRVPGTSLLYPDGF
CEEEEEECCCCCEEECCCEECCCCCCCCCEEEHHHHHHHHCCCCCEEECCCCEEECCCCC
LPVIEPYIWDGSGTLGISADLGGFALDLSQTYGRNEADFRVFDTVNVSLGLDSPTSFDSG
HHHCCCEEECCCCCEEEEECCCCEEEEEHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCC
GVTYEQYVTDFGVSRSLGLLAGGNITAGAQYRRETYAIREGEPAASTGVGADGFAGFAPR
CEEHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCC
RPVNEVRDAFAFYLDAELRPIEALLIGGAVRYDEYDDSGGETTWRVSARADLTEGLALRG
CCHHHHHHHHEEEECCCCCCEEEEEECCEEEECCCCCCCCCEEEEEEECCCCCCCEEEEC
AIGTAFRAPSLQQQNFSAVQGALSAGQLVSVATLPVSDPIARALGAGDLRPERSRNYSAG
CCCHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCC
IVLEPTPRLAFTLDAFHIQIDDRIALSEQLRGAAVNAILAGAGIVGFSQVRFFTNAVDTR
EEECCCCCEEEEEEEEEEEECCCCHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
TTGVEATARWSASLSPETRASMDVGFALYDTELESLRANPVLPTLPLLGEKSILFLTEAQ
CCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCCCCCEEEEEECCC
PRDKLTVQAVVEHRSVSARAALTRFGTYTSQPLVETQTFDGGTSFDLSLSYALSSAIMLT
CCCCEEHEEHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHH
AGVQNLFDDRPEEIADQARFIAATGGSFPTGEETPLGVNGRSYFVQLRGNF
HHHHHHHCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA