Click here to switch to the map view.

The map label for this gene is estA [H]

Identifier: 84625438

GI number: 84625438

Start: 4275140

End: 4275808

Strand: Reverse

Name: estA [H]

Synonym: XOO_3781

Alternate gene names: 84625438

Gene position: 4275808-4275140 (Counterclockwise)

Preceding gene: 84625439

Following gene: 84625437

Centisome position: 86.55

GC content: 68.31

Gene sequence:

>669_bases
ATGATGCTGGAAGTGATCGAACGCGAGACCGGACCGAATCCACAATGGAGCGTGCTGTGGCTGCATGGCCTGGGCGCCGA
TGGCAGCGACTTCGCCCCGATGGTGCCCGAACTGGTGCGCCCGGATTGGCCGGCGCTGCGCTTCGTCTTCCCGCATGCGC
CGGTCCGCCCGATCACCATCAATAACGGTGTGCGCATGCGCGGCTGGTACGACATCGTCGGCATGGATTTCGCACAGCGC
GCCGACAAGGCCGGCATTGCCGAATCGGTGGCTCAGGTCGAAGCCTTGATCGCCCATGCGCAGTCGCGCGGCATTGCGCC
CGAACGCATCTTGCTGGCCGGCTTCTCGCAAGGCGGCGCCGTGACCCTGGCGGTGGGCCTGCAGCGCAGCGTGCCGCTGG
CCGGGCTGATCGCGCTGTCGACTTATCTGCCAGAGCCAACCGCGGTCGCCACCCAGCTGCAGCCAGCCGCAACCCGCCAG
CCGCTGTTCATGGCGCATGGCACGGCCGACCCGGTGGTGCCGTTCGCGGCGGGCCAGGCCAGCATGCAGACCTTGCGCAC
GCTCGGCTTCGCGCTGGACTGGCACAGCTACCCGATGGGCCACCAGGTTTGCCTGGAAGAGATCGAGGCCCTGCGCGACT
GGATGCAGGCGCGCTTCAGCGCCGCCTGA

Upstream 100 bases:

>100_bases
CAGTTGCAGCTGCAGTTGCATGCACGCATCGCAGTCTGCGCCGGCTGATCCGACCCGTTCGTCGCACAAGGCATAATGCC
CAGCCCGCAAGCGGAGCTGG

Downstream 100 bases:

>100_bases
GCCCATGGCCCAGCCCGCGCCACAGATTGTCTGGATGCCGGACCTGTGCCGGCTGCCGCGGTTGGGCGCGATGCTGGGCC
TGGCCGAACTGGTGGTGCTG

Product: carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MMLEVIERETGPNPQWSVLWLHGLGADGSDFAPMVPELVRPDWPALRFVFPHAPVRPITINNGVRMRGWYDIVGMDFAQR
ADKAGIAESVAQVEALIAHAQSRGIAPERILLAGFSQGGAVTLAVGLQRSVPLAGLIALSTYLPEPTAVATQLQPAATRQ
PLFMAHGTADPVVPFAAGQASMQTLRTLGFALDWHSYPMGHQVCLEEIEALRDWMQARFSAA

Sequences:

>Translated_222_residues
MMLEVIERETGPNPQWSVLWLHGLGADGSDFAPMVPELVRPDWPALRFVFPHAPVRPITINNGVRMRGWYDIVGMDFAQR
ADKAGIAESVAQVEALIAHAQSRGIAPERILLAGFSQGGAVTLAVGLQRSVPLAGLIALSTYLPEPTAVATQLQPAATRQ
PLFMAHGTADPVVPFAAGQASMQTLRTLGFALDWHSYPMGHQVCLEEIEALRDWMQARFSAA
>Mature_222_residues
MMLEVIERETGPNPQWSVLWLHGLGADGSDFAPMVPELVRPDWPALRFVFPHAPVRPITINNGVRMRGWYDIVGMDFAQR
ADKAGIAESVAQVEALIAHAQSRGIAPERILLAGFSQGGAVTLAVGLQRSVPLAGLIALSTYLPEPTAVATQLQPAATRQ
PLFMAHGTADPVVPFAAGQASMQTLRTLGFALDWHSYPMGHQVCLEEIEALRDWMQARFSAA

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI9966764, Length=213, Percent_Identity=33.8028169014084, Blast_Score=124, Evalue=5e-29,
Organism=Homo sapiens, GI5453722, Length=204, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI20270341, Length=209, Percent_Identity=27.7511961722488, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17508057, Length=205, Percent_Identity=32.1951219512195, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323147, Length=215, Percent_Identity=29.7674418604651, Blast_Score=106, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45553055, Length=201, Percent_Identity=33.8308457711443, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI45553057, Length=201, Percent_Identity=33.8308457711443, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21357257, Length=201, Percent_Identity=33.8308457711443, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161078179, Length=214, Percent_Identity=28.5046728971963, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24073; Mature: 24073

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLEVIERETGPNPQWSVLWLHGLGADGSDFAPMVPELVRPDWPALRFVFPHAPVRPITI
CCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCEEEE
NNGVRMRGWYDIVGMDFAQRADKAGIAESVAQVEALIAHAQSRGIAPERILLAGFSQGGA
CCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
VTLAVGLQRSVPLAGLIALSTYLPEPTAVATQLQPAATRQPLFMAHGTADPVVPFAAGQA
EEEEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHH
SMQTLRTLGFALDWHSYPMGHQVCLEEIEALRDWMQARFSAA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMLEVIERETGPNPQWSVLWLHGLGADGSDFAPMVPELVRPDWPALRFVFPHAPVRPITI
CCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCEEEE
NNGVRMRGWYDIVGMDFAQRADKAGIAESVAQVEALIAHAQSRGIAPERILLAGFSQGGA
CCCEEECEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
VTLAVGLQRSVPLAGLIALSTYLPEPTAVATQLQPAATRQPLFMAHGTADPVVPFAAGQA
EEEEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCHH
SMQTLRTLGFALDWHSYPMGHQVCLEEIEALRDWMQARFSAA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA