Click here to switch to the map view.

The map label for this gene is yogA [H]

Identifier: 84625246

GI number: 84625246

Start: 4059092

End: 4060069

Strand: Reverse

Name: yogA [H]

Synonym: XOO_3589

Alternate gene names: 84625246

Gene position: 4060069-4059092 (Counterclockwise)

Preceding gene: 84625250

Following gene: 84625245

Centisome position: 82.18

GC content: 66.97

Gene sequence:

>978_bases
ATGAGCGATACCACGATGACCGCCATCGCCATCCGCGATGGCAAGGGCGACGCCGACGCGCTGTACGCCACCGAGCAACC
GCGCCCGCGTCCTGCAGCGGGCCAGGTGCTGATGCGCGTGAACGCCGCCGGTATCAATCGCCCCGATCTGCTGCAACGCG
CCGGGCACTACCCGCCGCCGCCTGGCGCACCGGAAACGTTGGGGTTGGAAATCGCCGGCGAGATCGTGGAGCCGGCTGGT
CGCTGGAAAGTCGGCGAGCGCGTGTGCGCACTGCTGGGCGGCGGCGGCTATGCGCAATACGCCGCCGTCGATGCGCGTCA
CGTGCTGCCGATTCCTGCCGGTATGGATCTGGTGCACGCCGCGGCGCTGCCGGAAACCATCTTCACCGCCTACGCCAATC
TATTCGAACACGGTCGGCTCGCAGCAGGCGAATGGCTGTTGTTGCACGGTGCGACCTCGGGCATTGGCGTCACGGCGATC
CAGATGGCCAAGGCGGCAGGTGCGCATGTGCTGGCGACCGCACGCTCGGCCAGCAAGGCCGCACAGGCGCGCGCGTTGGG
TGCCGATGTGGCGATCGATTCCACCAGCGAATCGTTTGTCGAGGTGGCCAAGGCGCATGGCGGTATAGACGTCGCGCTCG
ACATGGTGGGCGCTGCAGTATTCGCCGACACCCTGGAAGCGCTCAATCAACGCGGACGTATCGTCTACATCGCCTCGCAG
GCCGGCGCCACCATCGAACTGCCGATTCCGGTGCTGATGCGCAAGCAGGCGGTCATCACCGGCTCGACGCTACGCCCACG
CACTGCGGATGAAAAGGCGCGCCTGGCGGCGGAAGTGGAGCGGGTTGTGTGGCCGTGGATCGCCGAAGGCAAGCTGCGGG
TGTTGGTCGACAAGCGCTTTCCGCTGGCCGAGGCTGCCGCGGCCCATCGCTACCTGGAGCAGGGGAGCCACCTGGGCAAG
ATCGTGCTGGAGATATAG

Upstream 100 bases:

>100_bases
CGCTGCTGCGGCAGCACCTGCAAGTAAACATGGGATGCATCTGCGCCAAGCGTTGACGTCTGCACGCCTACACTGGGCGC
ACCTTCCACGGAGCGTCTTC

Downstream 100 bases:

>100_bases
GCACACCGGCGCATCCGGTGCCGGACCGGGCGGACCCGCCGCCGGTTCGCTGGCGTGGACGCGCGCAGCCTTCGGTGTGT
AGCCGTCGGGGCACGGCAAA

Product: quinone reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSDTTMTAIAIRDGKGDADALYATEQPRPRPAAGQVLMRVNAAGINRPDLLQRAGHYPPPPGAPETLGLEIAGEIVEPAG
RWKVGERVCALLGGGGYAQYAAVDARHVLPIPAGMDLVHAAALPETIFTAYANLFEHGRLAAGEWLLLHGATSGIGVTAI
QMAKAAGAHVLATARSASKAAQARALGADVAIDSTSESFVEVAKAHGGIDVALDMVGAAVFADTLEALNQRGRIVYIASQ
AGATIELPIPVLMRKQAVITGSTLRPRTADEKARLAAEVERVVWPWIAEGKLRVLVDKRFPLAEAAAAHRYLEQGSHLGK
IVLEI

Sequences:

>Translated_325_residues
MSDTTMTAIAIRDGKGDADALYATEQPRPRPAAGQVLMRVNAAGINRPDLLQRAGHYPPPPGAPETLGLEIAGEIVEPAG
RWKVGERVCALLGGGGYAQYAAVDARHVLPIPAGMDLVHAAALPETIFTAYANLFEHGRLAAGEWLLLHGATSGIGVTAI
QMAKAAGAHVLATARSASKAAQARALGADVAIDSTSESFVEVAKAHGGIDVALDMVGAAVFADTLEALNQRGRIVYIASQ
AGATIELPIPVLMRKQAVITGSTLRPRTADEKARLAAEVERVVWPWIAEGKLRVLVDKRFPLAEAAAAHRYLEQGSHLGK
IVLEI
>Mature_324_residues
SDTTMTAIAIRDGKGDADALYATEQPRPRPAAGQVLMRVNAAGINRPDLLQRAGHYPPPPGAPETLGLEIAGEIVEPAGR
WKVGERVCALLGGGGYAQYAAVDARHVLPIPAGMDLVHAAALPETIFTAYANLFEHGRLAAGEWLLLHGATSGIGVTAIQ
MAKAAGAHVLATARSASKAAQARALGADVAIDSTSESFVEVAKAHGGIDVALDMVGAAVFADTLEALNQRGRIVYIASQA
GATIELPIPVLMRKQAVITGSTLRPRTADEKARLAAEVERVVWPWIAEGKLRVLVDKRFPLAEAAAAHRYLEQGSHLGKI
VLEI

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=325, Percent_Identity=37.8461538461538, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI22538444, Length=325, Percent_Identity=37.8461538461538, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI194239674, Length=304, Percent_Identity=30.9210526315789, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI13236495, Length=304, Percent_Identity=30.9210526315789, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI28557745, Length=324, Percent_Identity=30.5555555555556, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI194239676, Length=301, Percent_Identity=28.9036544850498, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI24308257, Length=335, Percent_Identity=26.2686567164179, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI18379349, Length=308, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI41872631, Length=305, Percent_Identity=28.1967213114754, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI47519420, Length=337, Percent_Identity=26.7062314540059, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI197927207, Length=195, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1790485, Length=318, Percent_Identity=31.7610062893082, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI226510941, Length=323, Percent_Identity=27.5541795665635, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507255, Length=331, Percent_Identity=28.0966767371601, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI212642053, Length=334, Percent_Identity=26.6467065868263, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536829, Length=242, Percent_Identity=27.6859504132231, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=329, Percent_Identity=26.4437689969605, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324486, Length=336, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323961, Length=309, Percent_Identity=29.4498381877023, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323729, Length=358, Percent_Identity=26.8156424581006, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319621, Length=285, Percent_Identity=27.0175438596491, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581345, Length=326, Percent_Identity=23.9263803680982, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19920632, Length=260, Percent_Identity=23.8461538461538, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221330659, Length=260, Percent_Identity=23.8461538461538, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34095; Mature: 33963

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTMTAIAIRDGKGDADALYATEQPRPRPAAGQVLMRVNAAGINRPDLLQRAGHYPPP
CCCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCC
PGAPETLGLEIAGEIVEPAGRWKVGERVCALLGGGGYAQYAAVDARHVLPIPAGMDLVHA
CCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCEEEECCCCHHHHHH
AALPETIFTAYANLFEHGRLAAGEWLLLHGATSGIGVTAIQMAKAAGAHVLATARSASKA
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHH
AQARALGADVAIDSTSESFVEVAKAHGGIDVALDMVGAAVFADTLEALNQRGRIVYIASQ
HHHHHCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
AGATIELPIPVLMRKQAVITGSTLRPRTADEKARLAAEVERVVWPWIAEGKLRVLVDKRF
CCCEEEECCHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEEECCC
PLAEAAAAHRYLEQGSHLGKIVLEI
CHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
SDTTMTAIAIRDGKGDADALYATEQPRPRPAAGQVLMRVNAAGINRPDLLQRAGHYPPP
CCCEEEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCC
PGAPETLGLEIAGEIVEPAGRWKVGERVCALLGGGGYAQYAAVDARHVLPIPAGMDLVHA
CCCCCCCCCEEHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEECCCEEEECCCCHHHHHH
AALPETIFTAYANLFEHGRLAAGEWLLLHGATSGIGVTAIQMAKAAGAHVLATARSASKA
HHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEHHHHHHH
AQARALGADVAIDSTSESFVEVAKAHGGIDVALDMVGAAVFADTLEALNQRGRIVYIASQ
HHHHHCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
AGATIELPIPVLMRKQAVITGSTLRPRTADEKARLAAEVERVVWPWIAEGKLRVLVDKRF
CCCEEEECCHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCEECCCEEEEEEECCC
PLAEAAAAHRYLEQGSHLGKIVLEI
CHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]