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The map label for this gene is holB [H]

Identifier: 84622468

GI number: 84622468

Start: 880349

End: 881308

Strand: Direct

Name: holB [H]

Synonym: XOO_0811

Alternate gene names: 84622468

Gene position: 880349-881308 (Clockwise)

Preceding gene: 84622467

Following gene: 84622469

Centisome position: 17.82

GC content: 68.54

Gene sequence:

>960_bases
ATGACCGCCGCATTTGCACCCTGGCAGCAGCGTGCCTTCGACCAGACCGTGGCCGCGCTGGATGCGGGACGGCTCGGGCA
TGGGCTGCTGATCTGCGGCCCGGAAGGTCTGGGCAAGCGTGCCGTCGCCCTGGCCCTGGCCGAGCACGTGCTGGCCAGCT
CGCCGGACCCGGCACTGGCGCAGCGCACGCGCCAGTTGATCGCCGCCGGTACCCATCCGGACCTGCAGCTGATCTCGTTC
GTTCCCAATCGCACCGGCGACAAGCTGCGCACGGAAATCGTCATCGAACAGGTGCGCGAGATTTCGCAGAAGCTCGCGCT
CACGCCGCACGACGGCATTGCCCAGGTGGTGATCGTCGACCCGGCCGATGCGATCAATCGCTCGGCCTGCAACGCCTTGC
TCAAGACCCTGGAAGAACCCTCGCCCGGCCGTTACCTGTGGTTGATCAGTGCGCAGCCGGCGCGGCTGCCGGCGACCATC
CGCAGCCGCTGTCAGCGCCTGGAATTCAAGCTGCCGCCCGCGCACGAAGCCCTTGCCTGGCTGCTGTCGCAAGGCGTGAG
CGAGCGCGCCGCGCAGGAAGGGCTGGATGCCGCGCGCGGCCATCCCGGCCTGGCGGCGCAGTGGTTGCGCGAAGACGGCC
TGGCCGTGCGGCGTGCGGTTGCGCAGGATCTGGAGCAGATTGCCAGTGGCCGCGCCGGCGCAGTCGATGTCGCGCAGCGC
TGGACCAACGACGGCCAGGCCGATCAACGCCTGCGCCATGCTGCCGATCTGGTATTGGCGCAGGCATCTGCCGGCTTGAC
CGATCCATCGCGGTTGCACAAGCTGGTGACCTGGTTCGACGCCGCCAACCGCACCCGCGATCTGCTGCGCACCACCGTCC
GCGCCGACCTAGCAGTGACGGAATTATTGCTGTCCTGGCGCGAGGGAGAGCGCCAGCCACGTTCTAGGGGAACTCGATGA

Upstream 100 bases:

>100_bases
CGCATGCATTCTCGGCCACGCTGGCCGAACATAACGCAGCGGTGGCACGCTATCTGCAGCGCCGCCGGCTGCAAAGCACG
CCGCAAACCGAGCCCACGCC

Downstream 100 bases:

>100_bases
GTGCAATGAATGCACGCCAAGGCATTTTGTCGCTGGCGTTGAAAGACAAGCCAGCGCTTTACAGCGCCTACATGCCGTTC
GTGAAAGGCGGCGGCATCTT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTAAFAPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALAQRTRQLIAAGTHPDLQLISF
VPNRTGDKLRTEIVIEQVREISQKLALTPHDGIAQVVIVDPADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATI
RSRCQRLEFKLPPAHEALAWLLSQGVSERAAQEGLDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIASGRAGAVDVAQR
WTNDGQADQRLRHAADLVLAQASAGLTDPSRLHKLVTWFDAANRTRDLLRTTVRADLAVTELLLSWREGERQPRSRGTR

Sequences:

>Translated_319_residues
MTAAFAPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALAQRTRQLIAAGTHPDLQLISF
VPNRTGDKLRTEIVIEQVREISQKLALTPHDGIAQVVIVDPADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATI
RSRCQRLEFKLPPAHEALAWLLSQGVSERAAQEGLDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIASGRAGAVDVAQR
WTNDGQADQRLRHAADLVLAQASAGLTDPSRLHKLVTWFDAANRTRDLLRTTVRADLAVTELLLSWREGERQPRSRGTR
>Mature_318_residues
TAAFAPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALAQRTRQLIAAGTHPDLQLISFV
PNRTGDKLRTEIVIEQVREISQKLALTPHDGIAQVVIVDPADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATIR
SRCQRLEFKLPPAHEALAWLLSQGVSERAAQEGLDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIASGRAGAVDVAQRW
TNDGQADQRLRHAADLVLAQASAGLTDPSRLHKLVTWFDAANRTRDLLRTTVRADLAVTELLLSWREGERQPRSRGTR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=326, Percent_Identity=31.2883435582822, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1786676, Length=167, Percent_Identity=29.940119760479, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34703; Mature: 34572

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAFAPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHH
QRTRQLIAAGTHPDLQLISFVPNRTGDKLRTEIVIEQVREISQKLALTPHDGIAQVVIVD
HHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATIRSRCQRLEFKLPPAHEALAW
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LLSQGVSERAAQEGLDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIASGRAGAVDVAQR
HHHHCHHHHHHHHCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
WTNDGQADQRLRHAADLVLAQASAGLTDPSRLHKLVTWFDAANRTRDLLRTTVRADLAVT
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLLSWREGERQPRSRGTR
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TAAFAPWQQRAFDQTVAALDAGRLGHGLLICGPEGLGKRAVALALAEHVLASSPDPALA
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHH
QRTRQLIAAGTHPDLQLISFVPNRTGDKLRTEIVIEQVREISQKLALTPHDGIAQVVIVD
HHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PADAINRSACNALLKTLEEPSPGRYLWLISAQPARLPATIRSRCQRLEFKLPPAHEALAW
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LLSQGVSERAAQEGLDAARGHPGLAAQWLREDGLAVRRAVAQDLEQIASGRAGAVDVAQR
HHHHCHHHHHHHHCHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHH
WTNDGQADQRLRHAADLVLAQASAGLTDPSRLHKLVTWFDAANRTRDLLRTTVRADLAVT
HCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLLSWREGERQPRSRGTR
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]