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The map label for this gene is rsuA [H]

Identifier: 84622298

GI number: 84622298

Start: 694163

End: 694876

Strand: Direct

Name: rsuA [H]

Synonym: XOO_0641

Alternate gene names: 84622298

Gene position: 694163-694876 (Clockwise)

Preceding gene: 84622297

Following gene: 84622299

Centisome position: 14.05

GC content: 63.87

Gene sequence:

>714_bases
ATGAAGCTGGTCAAACATATCGCCAATCTCGGTTATGGCAGCCGTAAGCAGGTGACCCAGCTGTTTCGACAGGGCGCGGT
CACCGATGCGCAGGGCGAGGTGCTGTACGCCGACGACCAGGTGGAGCACGACGCCATCCGCATCGATGGCGAACCGCTCG
ATCCGCCACCGGGCTTCAGCCTGTTGCTGCATAAACCCGGCGGCTACACCTGTTCGACCAAGGACACCGGCCGGCTGATC
TACGAGCTGTTGCCGCCACGCTTCCGTTCGCGTGCGCCGGTGCTGGCGCCGGTGGGCCGGCTGGACCGCGAAACCAGCGG
CATGTTGTTGATGACCGACGACGGCGCGCTGCTGCATCGGATCATTTCGCCCAAATCCGCCCTGGACAAGGTGTACGACA
TCACCCTGGCCGAAGAGCTGCGTGGCGACGAAGCCGCGTTGTTTGCCAGTGGCAGCTTGCTGTTGGAAGGCGAAACCAAG
CCGCTGTTGCCGGCCGAATTGGAGGTCCTTGGCCCACGCGAAGCGCGGCTGACCCTGCACGAAGGCCGCTACCACCAAGT
ACGGCGCATGTTCGCTGCCGCCGGCAATCACGTGGTTGCTCTACATCGCAGCCGCATTGGCGGCCTGTCGCTGGATGGAT
TGCCGTCCGGGCAATGGCGTGCGCTGGAAGCGAGCGATCTTGAGGTGTTGTTTGGTCGCGGTGCTGCCGCATGA

Upstream 100 bases:

>100_bases
ACGAACAGGTGCTCAACGAAAGTTTCGGGCAGGTGCGCGTGGCTGCAGAGCGCGATGGTTTCAAGCTGATCTCGGCCATC
CGCGGCCGTGGAGCGCGCGC

Downstream 100 bases:

>100_bases
CGCAGATTGCAGCGCTGTCATTCCGCCCGCAGGCGGTGATCTTCGACATGGATGGCTTGATGCTCGACAGCGAGCGCGCC
ATCACCGCCTGCCTGGTGCA

Product: ribosomal small subunit pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKLVKHIANLGYGSRKQVTQLFRQGAVTDAQGEVLYADDQVEHDAIRIDGEPLDPPPGFSLLLHKPGGYTCSTKDTGRLI
YELLPPRFRSRAPVLAPVGRLDRETSGMLLMTDDGALLHRIISPKSALDKVYDITLAEELRGDEAALFASGSLLLEGETK
PLLPAELEVLGPREARLTLHEGRYHQVRRMFAAAGNHVVALHRSRIGGLSLDGLPSGQWRALEASDLEVLFGRGAAA

Sequences:

>Translated_237_residues
MKLVKHIANLGYGSRKQVTQLFRQGAVTDAQGEVLYADDQVEHDAIRIDGEPLDPPPGFSLLLHKPGGYTCSTKDTGRLI
YELLPPRFRSRAPVLAPVGRLDRETSGMLLMTDDGALLHRIISPKSALDKVYDITLAEELRGDEAALFASGSLLLEGETK
PLLPAELEVLGPREARLTLHEGRYHQVRRMFAAAGNHVVALHRSRIGGLSLDGLPSGQWRALEASDLEVLFGRGAAA
>Mature_237_residues
MKLVKHIANLGYGSRKQVTQLFRQGAVTDAQGEVLYADDQVEHDAIRIDGEPLDPPPGFSLLLHKPGGYTCSTKDTGRLI
YELLPPRFRSRAPVLAPVGRLDRETSGMLLMTDDGALLHRIISPKSALDKVYDITLAEELRGDEAALFASGSLLLEGETK
PLLPAELEVLGPREARLTLHEGRYHQVRRMFAAAGNHVVALHRSRIGGLSLDGLPSGQWRALEASDLEVLFGRGAAA

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=190, Percent_Identity=46.8421052631579, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=29.2372881355932, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=30.9236947791165, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87081838, Length=143, Percent_Identity=34.2657342657343, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 25817; Mature: 25817

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVKHIANLGYGSRKQVTQLFRQGAVTDAQGEVLYADDQVEHDAIRIDGEPLDPPPGFS
CHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCE
LLLHKPGGYTCSTKDTGRLIYELLPPRFRSRAPVLAPVGRLDRETSGMLLMTDDGALLHR
EEEECCCCEEECCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHH
IISPKSALDKVYDITLAEELRGDEAALFASGSLLLEGETKPLLPAELEVLGPREARLTLH
HHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHEEECCCCEEEEEE
EGRYHQVRRMFAAAGNHVVALHRSRIGGLSLDGLPSGQWRALEASDLEVLFGRGAAA
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MKLVKHIANLGYGSRKQVTQLFRQGAVTDAQGEVLYADDQVEHDAIRIDGEPLDPPPGFS
CHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCE
LLLHKPGGYTCSTKDTGRLIYELLPPRFRSRAPVLAPVGRLDRETSGMLLMTDDGALLHR
EEEECCCCEEECCCCHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHH
IISPKSALDKVYDITLAEELRGDEAALFASGSLLLEGETKPLLPAELEVLGPREARLTLH
HHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHEEECCCCEEEEEE
EGRYHQVRRMFAAAGNHVVALHRSRIGGLSLDGLPSGQWRALEASDLEVLFGRGAAA
CCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]