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The map label for this gene is amtB [C]

Identifier: 84489472

GI number: 84489472

Start: 774206

End: 775429

Strand: Direct

Name: amtB [C]

Synonym: Msp_0663

Alternate gene names: 84489472

Gene position: 774206-775429 (Clockwise)

Preceding gene: 84489471

Following gene: 84489473

Centisome position: 43.8

GC content: 36.6

Gene sequence:

>1224_bases
GTGGTAGCAGAATCGTTACTAAACACAGGTGATACGGCCTGGATACTAATGTCAACGGCATTAGTTATGATTATGACCCT
ACCAGGGTTAGCATTATTTTATGGAGGTATGAGCAAAAATAAAAACGTTCTTAACACAATGTTCTTATCTCTAACCGGTT
TTGCCATCGCATCAGTGATTTGGATAGCATATGGATACCAATTCACATTTGGATCAACAATCGGCGGATTTATAGGAGCA
CCAACAAATTTCTTATTAACAGGAATTTCACTTGATATGCTTCATGTAGACACAACAATACCTGAATTATTATATGTAGT
GTTCCAAGCTACATTTGCAGCAATTACAGTTGCATTAATATCAGGAGCAGTAGTAGGAAGAATGAAAGCAAAAGCATGGA
TGGTTTTTGTAGCATTATGGTTAACATTAGTATATGTCCCAATAGCTCACTGGGTATGGGGTGGAGGATGGTTATTTAAC
CTTGGTGCATTAGACTTTGCAGGAGGAGCAGTAGTACACTTAAACTCAGGTATAGCAGCACTAGCATTAATAACCGTACT
TGGACCTAGAAAAGACAGACGTTTATTACCTCACCATTTAGGATATGCAGTAATAGGTGCAGCATTATTATGGTTTGGAT
GGTTTGGATTCAATGCAGGATCAGCTCTTGGAGCAAATGGATTAGCAGCAAATGCATTTATAACAACCAACACTGCAGCA
GCAATGGCAATGATTGCTTGGATATTATTAGATATCTGGAAAACAGGAAAACCAACAATACTTGGTGCAATAACAGGTGC
AGTAGCAGGACTTGTAGCAATTACACCTGCAGCAGGATTTGTAGATGTACCAGCATCATTAATAATTGGATTTACAACAG
TATTTGTATCATACTTCGGTATATCATACTTAAAACCAAAACTTGGATACGACGATGCATTAGATGCATTTGGAGTACAT
GGATTATCAGGAACATGGGGAGCAATACTTACAGGAGTATTTGCATGTCCTGCAATTAATGAAGCAGCAGGTTTATTATA
TGGTAATCCAAATCAATTAGTAATACAAATAATAAGTATAGTAGCAGTAGCATTATACTCATTTATAGTAACTTACATTA
TTGCTCAAGTACTCGATAAAACAATGGGTATAAGAGTAGATGAACAAACTGAAATAGAAGGTTTAGATGCAAACGAACAT
GAAGAAGTAGGTTACAGAATATAA

Upstream 100 bases:

>100_bases
TAATAAAAAAAGTAACAATAATAATATACTAAGGAAGAAAAATAGTGAAAACATAACCGCAAATAAAAATTTAATTTATA
TATAATATGGAGGATTTATA

Downstream 100 bases:

>100_bases
ACATAAGGATATGATACAATGAAACAAATTACAGCCATATTTAGAAATGAAAGACTAGATGCAGTAAAAGATGCACTAGA
AAAAATAAACTGTAAAGGAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MVAESLLNTGDTAWILMSTALVMIMTLPGLALFYGGMSKNKNVLNTMFLSLTGFAIASVIWIAYGYQFTFGSTIGGFIGA
PTNFLLTGISLDMLHVDTTIPELLYVVFQATFAAITVALISGAVVGRMKAKAWMVFVALWLTLVYVPIAHWVWGGGWLFN
LGALDFAGGAVVHLNSGIAALALITVLGPRKDRRLLPHHLGYAVIGAALLWFGWFGFNAGSALGANGLAANAFITTNTAA
AMAMIAWILLDIWKTGKPTILGAITGAVAGLVAITPAAGFVDVPASLIIGFTTVFVSYFGISYLKPKLGYDDALDAFGVH
GLSGTWGAILTGVFACPAINEAAGLLYGNPNQLVIQIISIVAVALYSFIVTYIIAQVLDKTMGIRVDEQTEIEGLDANEH
EEVGYRI

Sequences:

>Translated_407_residues
MVAESLLNTGDTAWILMSTALVMIMTLPGLALFYGGMSKNKNVLNTMFLSLTGFAIASVIWIAYGYQFTFGSTIGGFIGA
PTNFLLTGISLDMLHVDTTIPELLYVVFQATFAAITVALISGAVVGRMKAKAWMVFVALWLTLVYVPIAHWVWGGGWLFN
LGALDFAGGAVVHLNSGIAALALITVLGPRKDRRLLPHHLGYAVIGAALLWFGWFGFNAGSALGANGLAANAFITTNTAA
AMAMIAWILLDIWKTGKPTILGAITGAVAGLVAITPAAGFVDVPASLIIGFTTVFVSYFGISYLKPKLGYDDALDAFGVH
GLSGTWGAILTGVFACPAINEAAGLLYGNPNQLVIQIISIVAVALYSFIVTYIIAQVLDKTMGIRVDEQTEIEGLDANEH
EEVGYRI
>Mature_407_residues
MVAESLLNTGDTAWILMSTALVMIMTLPGLALFYGGMSKNKNVLNTMFLSLTGFAIASVIWIAYGYQFTFGSTIGGFIGA
PTNFLLTGISLDMLHVDTTIPELLYVVFQATFAAITVALISGAVVGRMKAKAWMVFVALWLTLVYVPIAHWVWGGGWLFN
LGALDFAGGAVVHLNSGIAALALITVLGPRKDRRLLPHHLGYAVIGAALLWFGWFGFNAGSALGANGLAANAFITTNTAA
AMAMIAWILLDIWKTGKPTILGAITGAVAGLVAITPAAGFVDVPASLIIGFTTVFVSYFGISYLKPKLGYDDALDAFGVH
GLSGTWGAILTGVFACPAINEAAGLLYGNPNQLVIQIISIVAVALYSFIVTYIIAQVLDKTMGIRVDEQTEIEGLDANEH
EEVGYRI

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=412, Percent_Identity=43.4466019417476, Blast_Score=310, Evalue=9e-86,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=28.7671232876712, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=432, Percent_Identity=27.3148148148148, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=424, Percent_Identity=28.3018867924528, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=26.8348623853211, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=426, Percent_Identity=34.9765258215962, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=413, Percent_Identity=34.1404358353511, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=408, Percent_Identity=30.8823529411765, Blast_Score=186, Evalue=8e-48,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=31.0843373493976, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43095; Mature: 43095

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAESLLNTGDTAWILMSTALVMIMTLPGLALFYGGMSKNKNVLNTMFLSLTGFAIASVI
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WIAYGYQFTFGSTIGGFIGAPTNFLLTGISLDMLHVDTTIPELLYVVFQATFAAITVALI
HHHHCCEEEECCHHHHHHCCCHHHHEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVVGRMKAKAWMVFVALWLTLVYVPIAHWVWGGGWLFNLGALDFAGGAVVHLNSGIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCEEEEECCCHHH
LALITVLGPRKDRRLLPHHLGYAVIGAALLWFGWFGFNAGSALGANGLAANAFITTNTAA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHH
AMAMIAWILLDIWKTGKPTILGAITGAVAGLVAITPAAGFVDVPASLIIGFTTVFVSYFG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHH
ISYLKPKLGYDDALDAFGVHGLSGTWGAILTGVFACPAINEAAGLLYGNPNQLVIQIISI
HHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHH
VAVALYSFIVTYIIAQVLDKTMGIRVDEQTEIEGLDANEHEEVGYRI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVAESLLNTGDTAWILMSTALVMIMTLPGLALFYGGMSKNKNVLNTMFLSLTGFAIASVI
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WIAYGYQFTFGSTIGGFIGAPTNFLLTGISLDMLHVDTTIPELLYVVFQATFAAITVALI
HHHHCCEEEECCHHHHHHCCCHHHHEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVVGRMKAKAWMVFVALWLTLVYVPIAHWVWGGGWLFNLGALDFAGGAVVHLNSGIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCEEEEECCCHHH
LALITVLGPRKDRRLLPHHLGYAVIGAALLWFGWFGFNAGSALGANGLAANAFITTNTAA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHH
AMAMIAWILLDIWKTGKPTILGAITGAVAGLVAITPAAGFVDVPASLIIGFTTVFVSYFG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHHHHHHHH
ISYLKPKLGYDDALDAFGVHGLSGTWGAILTGVFACPAINEAAGLLYGNPNQLVIQIISI
HHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHH
VAVALYSFIVTYIIAQVLDKTMGIRVDEQTEIEGLDANEHEEVGYRI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]