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The map label for this gene is frvX [C]

Identifier: 84489375

GI number: 84489375

Start: 656234

End: 657262

Strand: Direct

Name: frvX [C]

Synonym: Msp_0565

Alternate gene names: 84489375

Gene position: 656234-657262 (Clockwise)

Preceding gene: 84489372

Following gene: 84489378

Centisome position: 37.13

GC content: 33.14

Gene sequence:

>1029_bases
ATGAAAAGTTTATTAAAAAAATTATCTGAAGCTTGTGGAATTTCAGGCTTCGAAGATGAAGTACGTGAAATCTTAAAAGA
TGAATTAAAAGATCATGTTGATGATATGGAAACAGATATTATGGGTAATCTTATAACAACCCATAAAGGTAAAGAAGGAA
AACCTAGTGTAATGTTAGCTTCACATATGGATGAAATTGGTCTTATGGTAAGTTTCATTGATGATGATGGATATTTAAGA
TTTGTTAAAATTGGTGGAATCAACGATCAAATGTTATTAAATCAGAAAGTGTATGTACAAACAGAAAATGGTGAAGTACC
TGGTATTATTGGTTCCAAACCACCTCATATTACAAGTGCTGCTGAAGCTAAAAAAATTATTCCATACAAAAATATGTTTA
TAGATATTGGAGCTAAAGACAAAGAACAAGCAGAAAGTCTTGTTTCTATTGGAGATGCTGTTGTATTCCATACTGAATTT
GAAGAATGTTTAAACGATCTTGTAATGGGTAAAGCATTAGATAACCGTGTAGGTTGTGCTGTTATGGCAGAAGTAATGAA
ACAAACAGATTGTGATGCAACAGTATATGGTGTTGGAACAGTACAGGAAGAAGTAGGATTAAAAGGAGCTAAAACATCAG
CATTCAAGTTAAATCCTGATATGGCTATTGCATTAGATGTAACCATTGCTGGAGATCATCCTGGAGTAAAACCTGAAGAT
GCTCCTGTAAAAGCAGGAAAAGGTCCTGTAATATCATTTGCTGATGCTAGTGGTAGAGGATTATTAACTCATCCTATGAT
GAAAAAATTATTAGTTGAAGCTGCTGAAGCTGCTGATATTGATTACCAAATAGAAGTAGGTGATGGTGGAACAACTGATG
CAACAGCAATTCACCTTACTCGTGAAGGTATAGCAACTGCAACTTTATCAAGTGGTTCAAGATATATTCACACACCTATT
AGTGTTGTAAATGTAAAAGATTTAGAAGACACAGTAAAACTCATACTTGAATTCTTAAAAAGATTATAA

Upstream 100 bases:

>100_bases
AATATAAGTAAATATAATATTATATATACAGAAAATGTATAAAAAAATAGATTAATTTAATTATAAAGAATATAAAACCC
AATAAAAACGAGGTTATACT

Downstream 100 bases:

>100_bases
AATCTTTTTTTATTCTATTTTTTTAATTAAAATACTATTATCATATTTTATTCTCAAAAAAAAAGTTGGAAGGGGAATTT
TAGTGAATTTATTCCATATT

Product: M42 glutamyl aminopeptidase

Products: D-glucose; GDP

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKSLLKKLSEACGISGFEDEVREILKDELKDHVDDMETDIMGNLITTHKGKEGKPSVMLASHMDEIGLMVSFIDDDGYLR
FVKIGGINDQMLLNQKVYVQTENGEVPGIIGSKPPHITSAAEAKKIIPYKNMFIDIGAKDKEQAESLVSIGDAVVFHTEF
EECLNDLVMGKALDNRVGCAVMAEVMKQTDCDATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTIAGDHPGVKPED
APVKAGKGPVISFADASGRGLLTHPMMKKLLVEAAEAADIDYQIEVGDGGTTDATAIHLTREGIATATLSSGSRYIHTPI
SVVNVKDLEDTVKLILEFLKRL

Sequences:

>Translated_342_residues
MKSLLKKLSEACGISGFEDEVREILKDELKDHVDDMETDIMGNLITTHKGKEGKPSVMLASHMDEIGLMVSFIDDDGYLR
FVKIGGINDQMLLNQKVYVQTENGEVPGIIGSKPPHITSAAEAKKIIPYKNMFIDIGAKDKEQAESLVSIGDAVVFHTEF
EECLNDLVMGKALDNRVGCAVMAEVMKQTDCDATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTIAGDHPGVKPED
APVKAGKGPVISFADASGRGLLTHPMMKKLLVEAAEAADIDYQIEVGDGGTTDATAIHLTREGIATATLSSGSRYIHTPI
SVVNVKDLEDTVKLILEFLKRL
>Mature_342_residues
MKSLLKKLSEACGISGFEDEVREILKDELKDHVDDMETDIMGNLITTHKGKEGKPSVMLASHMDEIGLMVSFIDDDGYLR
FVKIGGINDQMLLNQKVYVQTENGEVPGIIGSKPPHITSAAEAKKIIPYKNMFIDIGAKDKEQAESLVSIGDAVVFHTEF
EECLNDLVMGKALDNRVGCAVMAEVMKQTDCDATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTIAGDHPGVKPED
APVKAGKGPVISFADASGRGLLTHPMMKKLLVEAAEAADIDYQIEVGDGGTTDATAIHLTREGIATATLSSGSRYIHTPI
SVVNVKDLEDTVKLILEFLKRL

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family [H]

Homologues:

Organism=Escherichia coli, GI1790332, Length=342, Percent_Identity=38.0116959064327, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI87082417, Length=344, Percent_Identity=31.3953488372093, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1788727, Length=346, Percent_Identity=29.4797687861272, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008007 [H]

Pfam domain/function: PF05343 Peptidase_M42 [H]

EC number: 3.2.1.4

Molecular weight: Translated: 36831; Mature: 36831

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLLKKLSEACGISGFEDEVREILKDELKDHVDDMETDIMGNLITTHKGKEGKPSVMLA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCEEEH
SHMDEIGLMVSFIDDDGYLRFVKIGGINDQMLLNQKVYVQTENGEVPGIIGSKPPHITSA
HHHHHHCEEEEEECCCCCEEEEEECCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCH
AEAKKIIPYKNMFIDIGAKDKEQAESLVSIGDAVVFHTEFEECLNDLVMGKALDNRVGCA
HHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCHHHH
VMAEVMKQTDCDATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTIAGDHPGVKPED
HHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCEEEECCCEEEEEEEEEECCCCCCCCCC
APVKAGKGPVISFADASGRGLLTHPMMKKLLVEAAEAADIDYQIEVGDGGTTDATAIHLT
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEE
REGIATATLSSGSRYIHTPISVVNVKDLEDTVKLILEFLKRL
CCCCEEEEECCCCEEEECCEEEEEEHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKSLLKKLSEACGISGFEDEVREILKDELKDHVDDMETDIMGNLITTHKGKEGKPSVMLA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCEEEH
SHMDEIGLMVSFIDDDGYLRFVKIGGINDQMLLNQKVYVQTENGEVPGIIGSKPPHITSA
HHHHHHCEEEEEECCCCCEEEEEECCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCH
AEAKKIIPYKNMFIDIGAKDKEQAESLVSIGDAVVFHTEFEECLNDLVMGKALDNRVGCA
HHHHHCCCCCCEEEECCCCCHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHCCCHHHH
VMAEVMKQTDCDATVYGVGTVQEEVGLKGAKTSAFKLNPDMAIALDVTIAGDHPGVKPED
HHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCEEEECCCEEEEEEEEEECCCCCCCCCC
APVKAGKGPVISFADASGRGLLTHPMMKKLLVEAAEAADIDYQIEVGDGGTTDATAIHLT
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEE
REGIATATLSSGSRYIHTPISVVNVKDLEDTVKLILEFLKRL
CCCCEEEEECCCCEEEECCEEEEEEHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPglucose; H2O

Specific reaction: GDP-glucose + H2O = D-glucose + GDP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]