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The map label for this gene is ilvI [H]

Identifier: 84489346

GI number: 84489346

Start: 630863

End: 632674

Strand: Direct

Name: ilvI [H]

Synonym: Msp_0536

Alternate gene names: 84489346

Gene position: 630863-632674 (Clockwise)

Preceding gene: 84489345

Following gene: 84489347

Centisome position: 35.69

GC content: 30.91

Gene sequence:

>1812_bases
ATGAAAATAAGAGTAGCAGATTATATAGCAGAATTTTTAGCTGAAAACAACATAGATACATTATTTACAGTTGTAGGTGG
AGGTGCAATGCATCTTAATGATGGATTTGGTCATAATGAAGATATTAAATGTATCTATAATCATCATGAACAAGCATGTG
CAATAGCAGCAGAAGGTTATTATAGAATGTCAAATAAACTACCTGCTGTGTGTGTAACAACAGGACCTGGGGGAACAAAT
GCACTAACTGGAGTACTTGGAGCATATCTTGATTCAATACCAATGCTTGTAATATCAGGACAAGTAAAATATGAAATGAC
AGTAGATAGTACAGGGCTAGATCTTAGACAACTAGGGGATCAAGAATGGAATATTGTCTCAACAGTAGATTCAATGACAA
AATATGCACATATGATAAAAAATCCTAATGAAATAAAATATGTTCTTCAAAAAGCATTATATCTTGCTACTTCTGGAAGA
CCAGGACCATGTTGGATAGATGTTCCATTAGATATTCAGGGAGCTATTATTGATACTGATGATTTAATAGAATTTAATGA
AGATGAATTGGATATAGATTTAGGTGTTAAAGTAACAAAAGATACAATTGAAAATGTTATTTCCAAACTTAAATCTGCTA
AAAGACCAGTTATTTATGCTGGTAGTGCTATTAGAACAAATAATGCACATGATGAATTTTTAAAACTTGTAAATAAATTA
CAAATACCTGTTGTAAATGCATGGAATGCTACAGATTCACTTGAATATGATAATAAATTAACAGTAGGTTGTGGTGGATC
ATTTGGAGATAGACCAGCAAACTTCGCTGTACAAAATAGTGATTTAATACTATCCTTAGGTTGTAGATTAAGTACTAGAC
AAGTTTCATTTGCATATGATAAATGGGCATATGATGCATATAAGATAATGGTGGAAATTGATAAGGCTGAAATAGAAAAA
CCAACAATTAATATAGATATGCCAATACAATCAGATATTGGTGATTTCCTAGAAATATTTAATAAGGTACTTGATGAAGA
GTATATGGGCTATGAATTTAATTACAATCAATGGGTAGATAAATGTAATGAGTGGAAAAATAAGTATCCTGTATGTGATA
AATTAAAATACCAACAAAAAAGTCCTATAAATGTATATGCATTTCTAGATACATTATCTTCAAATATGATGGAATATGAG
AAGATCGTGGTTGCTAATGGTTCAGCATGTGCATGTATTCATGGATATAAACTTAAAAAAGGACAAAGATTGGTTGTAAA
TTCTGGTGTTGCTTCAATGGGATATGACCTGCCTGCTGCTTGTGGTGCATGTTTTGGTATTGGTAAAAAACGTCTTGTAT
GTGTAAGTGGAGATGGAAGTATTCAAATGAATCTCCAAGAATTACAAACAATAGTGCATCATAAACTACCTATTAAATTA
TTTGTAATAAATAACAATGGATATCAGTCAATAAGAATAACACAAAGATCATTCTTTGAAAGACCATTTGTTGGAATTGG
TGGAGATAGTGGTGATGTAAGCTTCCCACAAATGAAAAAAATAGCTAATGCTTATTCTATTCCATATTATTCATGTAATG
ATATTAGTAAGTTAGATGAAACTGTTAATAAAACATTAAATCATGATGGATATGTGATGTGTGAAATATTTGTAGATACA
AAACAGGAATTTGAACCAAAATCTGCATCTAAAAAGCTTCCAGATGGAAAGATGGTTTCAGCACCACTTGAAGATATGAA
ACCATTCCTACCAAGAGATGAACTTGAAGAAAATATGATTATTAAGAAGTAA

Upstream 100 bases:

>100_bases
TAGTTACAGAGGAAGAAGTTAATTTACTTGCAAATGGTAATGCTTCTGGACGAGTTCAACGATAATACATAAAAATAATT
TAATTCAAGGAGACAATATA

Downstream 100 bases:

>100_bases
GTATGTACTTAAATTGTTGTGGTATTTATGAGGTATATAATTACTGGTGCTACTGGTATGATTGCCATTAGTATTATGAA
TCTTTTAAAGGAAAATAATG

Product: thiamine pyrophosphate enzyme

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MKIRVADYIAEFLAENNIDTLFTVVGGGAMHLNDGFGHNEDIKCIYNHHEQACAIAAEGYYRMSNKLPAVCVTTGPGGTN
ALTGVLGAYLDSIPMLVISGQVKYEMTVDSTGLDLRQLGDQEWNIVSTVDSMTKYAHMIKNPNEIKYVLQKALYLATSGR
PGPCWIDVPLDIQGAIIDTDDLIEFNEDELDIDLGVKVTKDTIENVISKLKSAKRPVIYAGSAIRTNNAHDEFLKLVNKL
QIPVVNAWNATDSLEYDNKLTVGCGGSFGDRPANFAVQNSDLILSLGCRLSTRQVSFAYDKWAYDAYKIMVEIDKAEIEK
PTINIDMPIQSDIGDFLEIFNKVLDEEYMGYEFNYNQWVDKCNEWKNKYPVCDKLKYQQKSPINVYAFLDTLSSNMMEYE
KIVVANGSACACIHGYKLKKGQRLVVNSGVASMGYDLPAACGACFGIGKKRLVCVSGDGSIQMNLQELQTIVHHKLPIKL
FVINNNGYQSIRITQRSFFERPFVGIGGDSGDVSFPQMKKIANAYSIPYYSCNDISKLDETVNKTLNHDGYVMCEIFVDT
KQEFEPKSASKKLPDGKMVSAPLEDMKPFLPRDELEENMIIKK

Sequences:

>Translated_603_residues
MKIRVADYIAEFLAENNIDTLFTVVGGGAMHLNDGFGHNEDIKCIYNHHEQACAIAAEGYYRMSNKLPAVCVTTGPGGTN
ALTGVLGAYLDSIPMLVISGQVKYEMTVDSTGLDLRQLGDQEWNIVSTVDSMTKYAHMIKNPNEIKYVLQKALYLATSGR
PGPCWIDVPLDIQGAIIDTDDLIEFNEDELDIDLGVKVTKDTIENVISKLKSAKRPVIYAGSAIRTNNAHDEFLKLVNKL
QIPVVNAWNATDSLEYDNKLTVGCGGSFGDRPANFAVQNSDLILSLGCRLSTRQVSFAYDKWAYDAYKIMVEIDKAEIEK
PTINIDMPIQSDIGDFLEIFNKVLDEEYMGYEFNYNQWVDKCNEWKNKYPVCDKLKYQQKSPINVYAFLDTLSSNMMEYE
KIVVANGSACACIHGYKLKKGQRLVVNSGVASMGYDLPAACGACFGIGKKRLVCVSGDGSIQMNLQELQTIVHHKLPIKL
FVINNNGYQSIRITQRSFFERPFVGIGGDSGDVSFPQMKKIANAYSIPYYSCNDISKLDETVNKTLNHDGYVMCEIFVDT
KQEFEPKSASKKLPDGKMVSAPLEDMKPFLPRDELEENMIIKK
>Mature_603_residues
MKIRVADYIAEFLAENNIDTLFTVVGGGAMHLNDGFGHNEDIKCIYNHHEQACAIAAEGYYRMSNKLPAVCVTTGPGGTN
ALTGVLGAYLDSIPMLVISGQVKYEMTVDSTGLDLRQLGDQEWNIVSTVDSMTKYAHMIKNPNEIKYVLQKALYLATSGR
PGPCWIDVPLDIQGAIIDTDDLIEFNEDELDIDLGVKVTKDTIENVISKLKSAKRPVIYAGSAIRTNNAHDEFLKLVNKL
QIPVVNAWNATDSLEYDNKLTVGCGGSFGDRPANFAVQNSDLILSLGCRLSTRQVSFAYDKWAYDAYKIMVEIDKAEIEK
PTINIDMPIQSDIGDFLEIFNKVLDEEYMGYEFNYNQWVDKCNEWKNKYPVCDKLKYQQKSPINVYAFLDTLSSNMMEYE
KIVVANGSACACIHGYKLKKGQRLVVNSGVASMGYDLPAACGACFGIGKKRLVCVSGDGSIQMNLQELQTIVHHKLPIKL
FVINNNGYQSIRITQRSFFERPFVGIGGDSGDVSFPQMKKIANAYSIPYYSCNDISKLDETVNKTLNHDGYVMCEIFVDT
KQEFEPKSASKKLPDGKMVSAPLEDMKPFLPRDELEENMIIKK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=506, Percent_Identity=24.1106719367589, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1790104, Length=571, Percent_Identity=27.1453590192644, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=28.8224956063269, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786717, Length=505, Percent_Identity=26.3366336633663, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788716, Length=491, Percent_Identity=25.8655804480652, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=21.8914185639229, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=494, Percent_Identity=24.2914979757085, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=494, Percent_Identity=24.2914979757085, Blast_Score=99, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=28.125, Blast_Score=203, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6321524, Length=515, Percent_Identity=25.2427184466019, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320123, Length=630, Percent_Identity=21.9047619047619, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=512, Percent_Identity=24.21875, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=517, Percent_Identity=23.9845261121857, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320816, Length=500, Percent_Identity=24.2, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=23.936170212766, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 67313; Mature: 67313

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRVADYIAEFLAENNIDTLFTVVGGGAMHLNDGFGHNEDIKCIYNHHEQACAIAAEGY
CEEEHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCEEEECCCCCCEEEEECCC
YRMSNKLPAVCVTTGPGGTNALTGVLGAYLDSIPMLVISGQVKYEMTVDSTGLDLRQLGD
EECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCHHHCCC
QEWNIVSTVDSMTKYAHMIKNPNEIKYVLQKALYLATSGRPGPCWIDVPLDIQGAIIDTD
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCEEECCH
DLIEFNEDELDIDLGVKVTKDTIENVISKLKSAKRPVIYAGSAIRTNNAHDEFLKLVNKL
HCEECCCCCEEEEECCEECHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHH
QIPVVNAWNATDSLEYDNKLTVGCGGSFGDRPANFAVQNSDLILSLGCRLSTRQVSFAYD
CCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCEEEEECCCEECCEEEEEHH
KWAYDAYKIMVEIDKAEIEKPTINIDMPIQSDIGDFLEIFNKVLDEEYMGYEFNYNQWVD
HHCCEEEEEEEEECHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHCCEECCHHHHHH
KCNEWKNKYPVCDKLKYQQKSPINVYAFLDTLSSNMMEYEKIVVANGSACACIHGYKLKK
HHHHHHCCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHEEEEEEEECCCEEEEEECEEECC
GQRLVVNSGVASMGYDLPAACGACFGIGKKRLVCVSGDGSIQMNLQELQTIVHHKLPIKL
CCEEEEECCHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCEEE
FVINNNGYQSIRITQRSFFERPFVGIGGDSGDVSFPQMKKIANAYSIPYYSCNDISKLDE
EEEECCCCEEEEEEHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
TVNKTLNHDGYVMCEIFVDTKQEFEPKSASKKLPDGKMVSAPLEDMKPFLPRDELEENMI
HHHHHCCCCCEEEEEEEECCCHHCCCCCCCCCCCCCCEEECCHHHCCCCCCHHHHCCCCC
IKK
CCC
>Mature Secondary Structure
MKIRVADYIAEFLAENNIDTLFTVVGGGAMHLNDGFGHNEDIKCIYNHHEQACAIAAEGY
CEEEHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCEEEECCCCCCEEEEECCC
YRMSNKLPAVCVTTGPGGTNALTGVLGAYLDSIPMLVISGQVKYEMTVDSTGLDLRQLGD
EECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCEEEEEEECCCCCCHHHCCC
QEWNIVSTVDSMTKYAHMIKNPNEIKYVLQKALYLATSGRPGPCWIDVPLDIQGAIIDTD
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCEEECCH
DLIEFNEDELDIDLGVKVTKDTIENVISKLKSAKRPVIYAGSAIRTNNAHDEFLKLVNKL
HCEECCCCCEEEEECCEECHHHHHHHHHHHHHCCCCEEEECCEEECCCCHHHHHHHHHHH
QIPVVNAWNATDSLEYDNKLTVGCGGSFGDRPANFAVQNSDLILSLGCRLSTRQVSFAYD
CCCEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCEEEEECCCEECCEEEEEHH
KWAYDAYKIMVEIDKAEIEKPTINIDMPIQSDIGDFLEIFNKVLDEEYMGYEFNYNQWVD
HHCCEEEEEEEEECHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCHHHCCEECCHHHHHH
KCNEWKNKYPVCDKLKYQQKSPINVYAFLDTLSSNMMEYEKIVVANGSACACIHGYKLKK
HHHHHHCCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHEEEEEEEECCCEEEEEECEEECC
GQRLVVNSGVASMGYDLPAACGACFGIGKKRLVCVSGDGSIQMNLQELQTIVHHKLPIKL
CCEEEEECCHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHHCCCCEEE
FVINNNGYQSIRITQRSFFERPFVGIGGDSGDVSFPQMKKIANAYSIPYYSCNDISKLDE
EEEECCCCEEEEEEHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
TVNKTLNHDGYVMCEIFVDTKQEFEPKSASKKLPDGKMVSAPLEDMKPFLPRDELEENMI
HHHHHCCCCCEEEEEEEECCCHHCCCCCCCCCCCCCCEEECCHHHCCCCCCHHHHCCCCC
IKK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]