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The map label for this gene is rffG [C]

Identifier: 84489347

GI number: 84489347

Start: 632693

End: 633601

Strand: Direct

Name: rffG [C]

Synonym: Msp_0537

Alternate gene names: 84489347

Gene position: 632693-633601 (Clockwise)

Preceding gene: 84489346

Following gene: 84489348

Centisome position: 35.8

GC content: 31.24

Gene sequence:

>909_bases
GTGGTATTTATGAGGTATATAATTACTGGTGCTACTGGTATGATTGCCATTAGTATTATGAATCTTTTAAAGGAAAATAA
TGAAATATATGCAATAGTAAGGCCTGGTTCTTCAAAAATCAAGAATATTCCCGTGGATTCTAATATTCATGTTATTGAAT
GTGATGTAAGTGATTTATGTAATGTATCTCTTCCAGATTCTGATGTATTATTTCATCTTGCATGGAAGGGGACTATTGAT
GGACGTGATGATTCATATACTCAGCTTGATAATGTTAAATATACATTAGATGCATGTAAACTTGCAAAAAAATCTAATTG
TAGTGTATTTGTTGGTGCAGGTTCACAAGCAGAGTATGGATTAAAAGAAGAAGCATTAAATGGAAATATGTCAGTAGATC
CAGTAACAGGGTATGGTGTATGTAAGTATACTGCAGGTAAATTAAGTAGAATATTTGCACAAAACATGGGACTACGGCAT
ATTTGGGTTAGAATACTTAGTGTTTATGGTCCAGGAGATAATAAAAAAACACTCATATCTTCATGTATTGGTAGTATTCT
TAATAATAAACCATTTGACACAACAGAAGGAAATCAAATGTGGGATTACATATATTCTGAGGACTGTGCAAGGGCATTTA
TTAAAGTAGCAACAAGTGGTGTTGATGGTAAAGTTTATACCATTGGTTCTGGAAAAATTAGGCCTTTAAGAGAGTATATT
GAAATTATACGTGATAATATTAATCCGGATTTTAATGTTGGATTTGGAAGACGTGCTTATAATAAAAATCAGGTCATGTA
TCTTAAGGCAGATATTAGTGAACTTGTTATTGATACTGGATTTGTACCTCAAATAACATTTGAGGAAGGTATTAAAAGAA
CAATTAAATGGTTTAGAAGAAATAATTAA

Upstream 100 bases:

>100_bases
GATGGTTTCAGCACCACTTGAAGATATGAAACCATTCCTACCAAGAGATGAACTTGAAGAAAATATGATTATTAAGAAGT
AAGTATGTACTTAAATTGTT

Downstream 100 bases:

>100_bases
TTACGTGTTTAGGTGAGTTCTATGATTGAAGAAAAGATGGAAATAATATTAATCACATATAATCGAAGTAAGTACTTGGA
TAATACTTTAAATCAATTTT

Product: nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MVFMRYIITGATGMIAISIMNLLKENNEIYAIVRPGSSKIKNIPVDSNIHVIECDVSDLCNVSLPDSDVLFHLAWKGTID
GRDDSYTQLDNVKYTLDACKLAKKSNCSVFVGAGSQAEYGLKEEALNGNMSVDPVTGYGVCKYTAGKLSRIFAQNMGLRH
IWVRILSVYGPGDNKKTLISSCIGSILNNKPFDTTEGNQMWDYIYSEDCARAFIKVATSGVDGKVYTIGSGKIRPLREYI
EIIRDNINPDFNVGFGRRAYNKNQVMYLKADISELVIDTGFVPQITFEEGIKRTIKWFRRNN

Sequences:

>Translated_302_residues
MVFMRYIITGATGMIAISIMNLLKENNEIYAIVRPGSSKIKNIPVDSNIHVIECDVSDLCNVSLPDSDVLFHLAWKGTID
GRDDSYTQLDNVKYTLDACKLAKKSNCSVFVGAGSQAEYGLKEEALNGNMSVDPVTGYGVCKYTAGKLSRIFAQNMGLRH
IWVRILSVYGPGDNKKTLISSCIGSILNNKPFDTTEGNQMWDYIYSEDCARAFIKVATSGVDGKVYTIGSGKIRPLREYI
EIIRDNINPDFNVGFGRRAYNKNQVMYLKADISELVIDTGFVPQITFEEGIKRTIKWFRRNN
>Mature_302_residues
MVFMRYIITGATGMIAISIMNLLKENNEIYAIVRPGSSKIKNIPVDSNIHVIECDVSDLCNVSLPDSDVLFHLAWKGTID
GRDDSYTQLDNVKYTLDACKLAKKSNCSVFVGAGSQAEYGLKEEALNGNMSVDPVTGYGVCKYTAGKLSRIFAQNMGLRH
IWVRILSVYGPGDNKKTLISSCIGSILNNKPFDTTEGNQMWDYIYSEDCARAFIKVATSGVDGKVYTIGSGKIRPLREYI
EIIRDNINPDFNVGFGRRAYNKNQVMYLKADISELVIDTGFVPQITFEEGIKRTIKWFRRNN

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=318, Percent_Identity=23.2704402515723, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI48994969, Length=276, Percent_Identity=25.3623188405797, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788353, Length=179, Percent_Identity=27.3743016759777, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 33765; Mature: 33765

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFMRYIITGATGMIAISIMNLLKENNEIYAIVRPGSSKIKNIPVDSNIHVIECDVSDLC
CEEEEEEHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECHHHHC
NVSLPDSDVLFHLAWKGTIDGRDDSYTQLDNVKYTLDACKLAKKSNCSVFVGAGSQAEYG
CCCCCCCCEEEEEEEECCCCCCCCCCHHHCCEEEEHHHHHHHCCCCCEEEEECCCCCCCC
LKEEALNGNMSVDPVTGYGVCKYTAGKLSRIFAQNMGLRHIWVRILSVYGPGDNKKTLIS
CHHHHCCCCCEECCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
SCIGSILNNKPFDTTEGNQMWDYIYSEDCARAFIKVATSGVDGKVYTIGSGKIRPLREYI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHH
EIIRDNINPDFNVGFGRRAYNKNQVMYLKADISELVIDTGFVPQITFEEGIKRTIKWFRR
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCEEHHHHHHHHHHHHHHC
NN
CC
>Mature Secondary Structure
MVFMRYIITGATGMIAISIMNLLKENNEIYAIVRPGSSKIKNIPVDSNIHVIECDVSDLC
CEEEEEEHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECHHHHC
NVSLPDSDVLFHLAWKGTIDGRDDSYTQLDNVKYTLDACKLAKKSNCSVFVGAGSQAEYG
CCCCCCCCEEEEEEEECCCCCCCCCCHHHCCEEEEHHHHHHHCCCCCEEEEECCCCCCCC
LKEEALNGNMSVDPVTGYGVCKYTAGKLSRIFAQNMGLRHIWVRILSVYGPGDNKKTLIS
CHHHHCCCCCEECCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
SCIGSILNNKPFDTTEGNQMWDYIYSEDCARAFIKVATSGVDGKVYTIGSGKIRPLREYI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHH
EIIRDNINPDFNVGFGRRAYNKNQVMYLKADISELVIDTGFVPQITFEEGIKRTIKWFRR
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCEEHHHHHHHHHHHHHHC
NN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]