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The map label for this gene is rfbF [H]

Identifier: 84489341

GI number: 84489341

Start: 625801

End: 626577

Strand: Direct

Name: rfbF [H]

Synonym: Msp_0531

Alternate gene names: 84489341

Gene position: 625801-626577 (Clockwise)

Preceding gene: 84489338

Following gene: 84489342

Centisome position: 35.41

GC content: 29.99

Gene sequence:

>777_bases
ATGAAAGTGGTCATACTTGCTGGAGGCTTTGGAACTAGAATTTCTGAAGAATCCTATTTAAAACCTAAACCAATGATTGA
AATAGGGGAAAAACCAATTTTATGGCATATAATGAAAATATATTCCTACTATGGATATAATGAATTTATAATATGTCTTG
GATATAAATCCCACATGATTAAAGAATTTTTCGCAGATTACTATCTTCACACATCTGATGTAACATTTGACTTATCAAAA
AATGAAATGGAAGTACATAACAATTATTCAGAACCATGGAAAGTTACTTTAGTAGATACAGGTCTTAACACTATGACTGG
TGGACGTATTAAAAGAATTAAAGATTATTTGCCAGATGATGAACCATTCATGTTAACATATGGTGATGGGGTGTCTGATG
TTAATTTAAATAAATTATACAATTTCCATAAGAAAAATAAAAAACTTGCAACAATAACAGCAGTGAATCTTGCAGGACGA
TTTGGTGTACTAAAAATAGATGAAACTGAAACAATAAATAAATTCTCAGAAAAAACAAAAGAAGATGGTGGATGGATTAA
TGGTGGATTCATGATACTTGAAAAAGAAGCTATTGATTATATTACAGATGATACAACAGTTTTTGAAAAGGATCCACTTG
AAAACTTAGCAACAAATGGTCAGTTAATGGCATATAAGCATCATGGATTCTGGAAATGTATGGATACAAAAAGAGATCAT
GATGCTTTAGAAAAATTATGGCAAGAAGATAATGCACCATGGTATTTATGGAAATAA

Upstream 100 bases:

>100_bases
AAAAAATAATTTCATAAAAATATGTGAATTAATAAGGCTAACTACATATTATTAAAAAATAATATATTATATATAATAAA
AAAATAGGAGCATTAAATCT

Downstream 100 bases:

>100_bases
AGTATATAGTTGATAAATTATGGATAAATTCTTTAAAAATAAAAATATTTTACTAACAGGACATACAGGCTTTAAGGGTT
CATGGTTAACAAGTATATTA

Product: nucleoside-diphosphate-sugar pyrophosphorylase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKVVILAGGFGTRISEESYLKPKPMIEIGEKPILWHIMKIYSYYGYNEFIICLGYKSHMIKEFFADYYLHTSDVTFDLSK
NEMEVHNNYSEPWKVTLVDTGLNTMTGGRIKRIKDYLPDDEPFMLTYGDGVSDVNLNKLYNFHKKNKKLATITAVNLAGR
FGVLKIDETETINKFSEKTKEDGGWINGGFMILEKEAIDYITDDTTVFEKDPLENLATNGQLMAYKHHGFWKCMDTKRDH
DALEKLWQEDNAPWYLWK

Sequences:

>Translated_258_residues
MKVVILAGGFGTRISEESYLKPKPMIEIGEKPILWHIMKIYSYYGYNEFIICLGYKSHMIKEFFADYYLHTSDVTFDLSK
NEMEVHNNYSEPWKVTLVDTGLNTMTGGRIKRIKDYLPDDEPFMLTYGDGVSDVNLNKLYNFHKKNKKLATITAVNLAGR
FGVLKIDETETINKFSEKTKEDGGWINGGFMILEKEAIDYITDDTTVFEKDPLENLATNGQLMAYKHHGFWKCMDTKRDH
DALEKLWQEDNAPWYLWK
>Mature_258_residues
MKVVILAGGFGTRISEESYLKPKPMIEIGEKPILWHIMKIYSYYGYNEFIICLGYKSHMIKEFFADYYLHTSDVTFDLSK
NEMEVHNNYSEPWKVTLVDTGLNTMTGGRIKRIKDYLPDDEPFMLTYGDGVSDVNLNKLYNFHKKNKKLATITAVNLAGR
FGVLKIDETETINKFSEKTKEDGGWINGGFMILEKEAIDYITDDTTVFEKDPLENLATNGQLMAYKHHGFWKCMDTKRDH
DALEKLWQEDNAPWYLWK

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI133931050, Length=250, Percent_Identity=27.2, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320148, Length=251, Percent_Identity=29.0836653386454, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355443, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24644084, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 30035; Mature: 30035

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVILAGGFGTRISEESYLKPKPMIEIGEKPILWHIMKIYSYYGYNEFIICLGYKSHMI
CEEEEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHH
KEFFADYYLHTSDVTFDLSKNEMEVHNNYSEPWKVTLVDTGLNTMTGGRIKRIKDYLPDD
HHHHHHHEEECCCEEEEECCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC
EPFMLTYGDGVSDVNLNKLYNFHKKNKKLATITAVNLAGRFGVLKIDETETINKFSEKTK
CCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCHHHHHHHHHHH
EDGGWINGGFMILEKEAIDYITDDTTVFEKDPLENLATNGQLMAYKHHGFWKCMDTKRDH
HCCCEEECCEEEEEECHHHEECCCCEEECCCCHHHHHCCCEEEEEECCCEEEEECCCCCH
DALEKLWQEDNAPWYLWK
HHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MKVVILAGGFGTRISEESYLKPKPMIEIGEKPILWHIMKIYSYYGYNEFIICLGYKSHMI
CEEEEEECCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHH
KEFFADYYLHTSDVTFDLSKNEMEVHNNYSEPWKVTLVDTGLNTMTGGRIKRIKDYLPDD
HHHHHHHEEECCCEEEEECCCEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCC
EPFMLTYGDGVSDVNLNKLYNFHKKNKKLATITAVNLAGRFGVLKIDETETINKFSEKTK
CCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCHHHHHHHHHHH
EDGGWINGGFMILEKEAIDYITDDTTVFEKDPLENLATNGQLMAYKHHGFWKCMDTKRDH
HCCCEEECCEEEEEECHHHEECCCCEEECCCCHHHHHCCCEEEEEECCCEEEEECCCCCH
DALEKLWQEDNAPWYLWK
HHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]