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The map label for this gene is gshA

Identifier: 84489338

GI number: 84489338

Start: 623083

End: 624486

Strand: Direct

Name: gshA

Synonym: Msp_0528

Alternate gene names: 84489338

Gene position: 623083-624486 (Clockwise)

Preceding gene: 84489337

Following gene: 84489341

Centisome position: 35.25

GC content: 26.07

Gene sequence:

>1404_bases
ATGAAAAATTATTTTAATATAGAAGAGATAGAAAAAATACTTACTAACAATGAACTTCTATCAGGAAACTTTGGATTAGA
AAAGGAAGGTCTTAGAGTAACAAAAGATGGAAATCTATCCTTAACACCACATCCAGAAGTCTTTGGAGATAAACTTAAAA
ATCCATATATAACAACAGATTTCTCAGAAAGTCAGATAGAAATAGTAACACCATCATTCAATACATTAAATGAAGTATAT
CAATTTCTGTCATTTATAAGTGATTTAGTAAACATAAATATTCCAGATAATGAATATATATGGAATCAATCACTACCCTG
TATCATACCAGATGACTCTAAAATACCCATAGCCAAATATTCAAAAGAAGGTAAGGATGCATATGAATATAGAAAAAATC
TATCAAAAAAATATGGAAGAAAACTACAGTTAATATCAGGAATTCATTATAACTTCTCATTTGATGAAAAAGTCATAGAA
AAATTATATAAAAATATATCCCATGATCTTTCATATAAAAACTTTAAAAATCAGCTCTATCTTAAAATAGTAAGAAACTA
CCTTCGATATAAATGGCTAATAATATATCTAACAGGAACATCTATTGCATGTCATGAAACATTTATAGAAAAATGTTACA
ATCTAATGAATAAAAAAGATAACTATGATAGTTATTATGGTACTGAGGCTGTATCATATAGAAATGCATCATGTGGATAT
AAAAATAAAACACCCCTCTATCCTAGATACACATCAGTAGATGAGTTTATAAGAGATGTTAAAGATTTTATTAATAAAGG
TTTATTATCTGAATCAAAGGAATTATACACCCAGATACGTTTAAAACCACATGATAGAAATAACTTTTTAAAATCATTAA
AAGAGGATGGAATTCAATATATTGAAATAAGGACTATTGATATAAATCCATTTGATAAATGTGGAATATCTATGGAGGAT
ATGGAATTTATACACATATTCCTACTGTACTTGTTACTTAAAGAAGAATCCACTTATGATAAATGGCAGGAAGAAAGTTT
AATAAACGAGGAAAACACTGCAGAAAATGCATTTAATACTAATTTAAAACTATTAAATGATGGGTGTGAAGTATCACTAA
AGAATTGGGCTTTTAATATAATTAATGAAATTAAACAAATAAACACTAGACTAGATTTAGGTAAGGATGAAATTATACAT
ACTATGCAAGAGAGAATAGAAAATCCTAAAAAAACACATGCATATAAATTAAGAAATATTATTAAAAACAAGGGATATAT
TAACAGTCAACTATCAATTGCCAAAAACAATAAACAAACAAGTGAAGATATTTTTAACATGGAACATATTATGAAAAATG
AGAAACTCCTCAAATATTATAATGATGCCATAGAAAAAGTGTAA

Upstream 100 bases:

>100_bases
ATATTTTTAAAATTTTTTGATACATAACTTTTTATAATACTTTTAAAATATAACAATTGTTAAAAAATATTAATATAAAT
AATATACAAACATATAATAC

Downstream 100 bases:

>100_bases
ACTTCCATCTTAAATTCCTCTTAGATTTAGATATAAAACTTAATAAAATAGGATATTAACTAAAAAGATGTAAAAAGGAG
ATTAAATTAAATCTATTTCA

Product: GshA

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MKNYFNIEEIEKILTNNELLSGNFGLEKEGLRVTKDGNLSLTPHPEVFGDKLKNPYITTDFSESQIEIVTPSFNTLNEVY
QFLSFISDLVNINIPDNEYIWNQSLPCIIPDDSKIPIAKYSKEGKDAYEYRKNLSKKYGRKLQLISGIHYNFSFDEKVIE
KLYKNISHDLSYKNFKNQLYLKIVRNYLRYKWLIIYLTGTSIACHETFIEKCYNLMNKKDNYDSYYGTEAVSYRNASCGY
KNKTPLYPRYTSVDEFIRDVKDFINKGLLSESKELYTQIRLKPHDRNNFLKSLKEDGIQYIEIRTIDINPFDKCGISMED
MEFIHIFLLYLLLKEESTYDKWQEESLINEENTAENAFNTNLKLLNDGCEVSLKNWAFNIINEIKQINTRLDLGKDEIIH
TMQERIENPKKTHAYKLRNIIKNKGYINSQLSIAKNNKQTSEDIFNMEHIMKNEKLLKYYNDAIEKV

Sequences:

>Translated_467_residues
MKNYFNIEEIEKILTNNELLSGNFGLEKEGLRVTKDGNLSLTPHPEVFGDKLKNPYITTDFSESQIEIVTPSFNTLNEVY
QFLSFISDLVNINIPDNEYIWNQSLPCIIPDDSKIPIAKYSKEGKDAYEYRKNLSKKYGRKLQLISGIHYNFSFDEKVIE
KLYKNISHDLSYKNFKNQLYLKIVRNYLRYKWLIIYLTGTSIACHETFIEKCYNLMNKKDNYDSYYGTEAVSYRNASCGY
KNKTPLYPRYTSVDEFIRDVKDFINKGLLSESKELYTQIRLKPHDRNNFLKSLKEDGIQYIEIRTIDINPFDKCGISMED
MEFIHIFLLYLLLKEESTYDKWQEESLINEENTAENAFNTNLKLLNDGCEVSLKNWAFNIINEIKQINTRLDLGKDEIIH
TMQERIENPKKTHAYKLRNIIKNKGYINSQLSIAKNNKQTSEDIFNMEHIMKNEKLLKYYNDAIEKV
>Mature_467_residues
MKNYFNIEEIEKILTNNELLSGNFGLEKEGLRVTKDGNLSLTPHPEVFGDKLKNPYITTDFSESQIEIVTPSFNTLNEVY
QFLSFISDLVNINIPDNEYIWNQSLPCIIPDDSKIPIAKYSKEGKDAYEYRKNLSKKYGRKLQLISGIHYNFSFDEKVIE
KLYKNISHDLSYKNFKNQLYLKIVRNYLRYKWLIIYLTGTSIACHETFIEKCYNLMNKKDNYDSYYGTEAVSYRNASCGY
KNKTPLYPRYTSVDEFIRDVKDFINKGLLSESKELYTQIRLKPHDRNNFLKSLKEDGIQYIEIRTIDINPFDKCGISMED
MEFIHIFLLYLLLKEESTYDKWQEESLINEENTAENAFNTNLKLLNDGCEVSLKNWAFNIINEIKQINTRLDLGKDEIIH
TMQERIENPKKTHAYKLRNIIKNKGYINSQLSIAKNNKQTSEDIFNMEHIMKNEKLLKYYNDAIEKV

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=342, Percent_Identity=33.0409356725146, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR007370
- InterPro:   IPR006335
- InterPro:   IPR020561 [H]

Pfam domain/function: PF01071 GARS_A; PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 55109; Mature: 55109

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYFNIEEIEKILTNNELLSGNFGLEKEGLRVTKDGNLSLTPHPEVFGDKLKNPYITTD
CCCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEECCCCEEECCCCHHHHHHHCCCEEEEC
FSESQIEIVTPSFNTLNEVYQFLSFISDLVNINIPDNEYIWNQSLPCIIPDDSKIPIAKY
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCCCEEECCCCCCCHHHC
SKEGKDAYEYRKNLSKKYGRKLQLISGIHYNFSFDEKVIEKLYKNISHDLSYKNFKNQLY
CCCCCHHHHHHHHHHHHHCCEEEEHHCEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LKIVRNYLRYKWLIIYLTGTSIACHETFIEKCYNLMNKKDNYDSYYGTEAVSYRNASCGY
HHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCC
KNKTPLYPRYTSVDEFIRDVKDFINKGLLSESKELYTQIRLKPHDRNNFLKSLKEDGIQY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
IEIRTIDINPFDKCGISMEDMEFIHIFLLYLLLKEESTYDKWQEESLINEENTAENAFNT
EEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHCC
NLKLLNDGCEVSLKNWAFNIINEIKQINTRLDLGKDEIIHTMQERIENPKKTHAYKLRNI
CCEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
IKNKGYINSQLSIAKNNKQTSEDIFNMEHIMKNEKLLKYYNDAIEKV
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNYFNIEEIEKILTNNELLSGNFGLEKEGLRVTKDGNLSLTPHPEVFGDKLKNPYITTD
CCCCCCHHHHHHHHCCCCEEECCCCCCCCCCEEECCCCEEECCCCHHHHHHHCCCEEEEC
FSESQIEIVTPSFNTLNEVYQFLSFISDLVNINIPDNEYIWNQSLPCIIPDDSKIPIAKY
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCEEECCCCCEEECCCCCCCHHHC
SKEGKDAYEYRKNLSKKYGRKLQLISGIHYNFSFDEKVIEKLYKNISHDLSYKNFKNQLY
CCCCCHHHHHHHHHHHHHCCEEEEHHCEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LKIVRNYLRYKWLIIYLTGTSIACHETFIEKCYNLMNKKDNYDSYYGTEAVSYRNASCGY
HHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCC
KNKTPLYPRYTSVDEFIRDVKDFINKGLLSESKELYTQIRLKPHDRNNFLKSLKEDGIQY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEE
IEIRTIDINPFDKCGISMEDMEFIHIFLLYLLLKEESTYDKWQEESLINEENTAENAFNT
EEEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHCC
NLKLLNDGCEVSLKNWAFNIINEIKQINTRLDLGKDEIIHTMQERIENPKKTHAYKLRNI
CCEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
IKNKGYINSQLSIAKNNKQTSEDIFNMEHIMKNEKLLKYYNDAIEKV
HHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]