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The map label for this gene is rfcL

Identifier: 84489226

GI number: 84489226

Start: 475831

End: 477435

Strand: Direct

Name: rfcL

Synonym: Msp_0414

Alternate gene names: 84489226

Gene position: 475831-477435 (Clockwise)

Preceding gene: 84489225

Following gene: 84489227

Centisome position: 26.92

GC content: 27.79

Gene sequence:

>1605_bases
TTGAAATGGGTAGAAAAATATGCACCTAAAAAATTAGGTGACGTACTTGGAAATGCCAAAGCAAAGGCACAGATAGAAGT
ATGGGCAAATAAATGGTCAAAAGGTGTTCCACAAAAACCATTATTATTAATGGGTCCTCCAGGAATTGGAAAAACCACAA
TTGCACATTTAGTTGGAAAAGAATATTTTTCTGAAACAATAGAAGTTAATGCTAGTGATAAAAGATCATATGATATTATA
AAAAGTAGTATTGGTGAAGCTGCACAAACTAGAAGTCTTTTTCATTCAGGATATAAATTGTTAATAATGGATGAAGTTGA
TGGAATAAGTGGACGTGATGATTCTGGTGGTGCACGTGCAGTAAATGAAACTATTAAGAATTCAAAACAACCAATAATTT
TAATGGCCAATGATGCATATAGTAAACGTTTAACTTCTATTAAACCTAAATGTCAAGGAATAAAGTTTACAAAAGTTCAT
ACAAATTCAATCAATGCACAATTAAAAAGAATATGTGCAAGAGAAGATATAGAATATGATTCAGAAGCATTATATACATT
AAGTAAGGAATCAAATGGTGATTTAAGATCTGCAATAACAAGTTTAGAAGCTATTGTTGATAATGATAAAAAAATTACAA
AGGATAGTTTATCTGTTATAGCAAAAAAAGATGGTGAACAAAATATTTTTGATACTGTTGTAGCAGTTTTAAAAAGTAAA
AATCCAGAGCATGTTGCTGAAGCTATGAGAGTTAATACTCAGCCACCATTTCTAATAGAATTAATTGCAGAAAATATTCC
AAGAGAGTATGAAAGAACAAATGAAATAGTAAAGGCTTATGAAATGATTTCTCTTGCTGATGTTAATTTGGGAAGAGCAT
TCAGAACACAAAACTACACATACTGGAAATATGCCTTTTTATTCATGGGACGAGGAGTAGCAGCAGCAAAAAAACAAACA
TATAAGAAGTTTTCAAGGATTATAACACCAACAATATACACTAAACTTTCAAAATCAAGAAAAAATAGAAATTTAAAAGA
ACAAGTAACACAGAAGATGAGTTTAAAACTACATACTTCTCCTAAGGAATTGGAAAAACAATTAGTATTCTATGAAGAAT
TGTTTAAAGATAATGAACAAGCATATGATTTAAAACAATATTTTAAATTAACAGATGATGAAGTAAAACTTTTCAGATCA
AGAAAAATACCAGCTTCTGTAGAGAAAAAACGTTTAACAAAACTTAGAAAAGAACAAGAACTTGAAGAAAAAGAAATTCA
AAAACAAAAGATAATAGATGCTAAAAAAGTAGAACTTAAAGAAGAATCTAAAAATAAAAAAGAAATTAAAGTTAAAACTA
AAAAAGACACTGTTGAGGATAGTTCTAAAACAAAATCTACAACTAAATCAAAGAAAACTAGTAAATCAACACCAACAAAA
AAAGTTACAAAAACTAAAAAATCTTCAAATTCCACAACTAAAAATAAGACAGAATCACCTAAAAATTCTAGTAAAACTTC
ATCTAAAACAAGTGTTGATGATAAAAAAACTTCTAAGAAAAATAATAAAAAGAAATCAAGACAAACTACACTATTTGATT
TCTAA

Upstream 100 bases:

>100_bases
TGAGTGAATGTGATTATAGAATAAGAGAAGGTTCTAATCCAAGACTTCAATTAGAAGCTTTATTAAGTAAATTTTTATTA
GTTAAACAGGATGCATAAAA

Downstream 100 bases:

>100_bases
TTTTACCTCTTTTTTTGTTGATTATAGTATACATTCCATAAAATTATTAGAATTAGATAATATACATATCTACTATAATA
ATTTAAAAGAAATTAGGATG

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKWVEKYAPKKLGDVLGNAKAKAQIEVWANKWSKGVPQKPLLLMGPPGIGKTTIAHLVGKEYFSETIEVNASDKRSYDII
KSSIGEAAQTRSLFHSGYKLLIMDEVDGISGRDDSGGARAVNETIKNSKQPIILMANDAYSKRLTSIKPKCQGIKFTKVH
TNSINAQLKRICAREDIEYDSEALYTLSKESNGDLRSAITSLEAIVDNDKKITKDSLSVIAKKDGEQNIFDTVVAVLKSK
NPEHVAEAMRVNTQPPFLIELIAENIPREYERTNEIVKAYEMISLADVNLGRAFRTQNYTYWKYAFLFMGRGVAAAKKQT
YKKFSRIITPTIYTKLSKSRKNRNLKEQVTQKMSLKLHTSPKELEKQLVFYEELFKDNEQAYDLKQYFKLTDDEVKLFRS
RKIPASVEKKRLTKLRKEQELEEKEIQKQKIIDAKKVELKEESKNKKEIKVKTKKDTVEDSSKTKSTTKSKKTSKSTPTK
KVTKTKKSSNSTTKNKTESPKNSSKTSSKTSVDDKKTSKKNNKKKSRQTTLFDF

Sequences:

>Translated_534_residues
MKWVEKYAPKKLGDVLGNAKAKAQIEVWANKWSKGVPQKPLLLMGPPGIGKTTIAHLVGKEYFSETIEVNASDKRSYDII
KSSIGEAAQTRSLFHSGYKLLIMDEVDGISGRDDSGGARAVNETIKNSKQPIILMANDAYSKRLTSIKPKCQGIKFTKVH
TNSINAQLKRICAREDIEYDSEALYTLSKESNGDLRSAITSLEAIVDNDKKITKDSLSVIAKKDGEQNIFDTVVAVLKSK
NPEHVAEAMRVNTQPPFLIELIAENIPREYERTNEIVKAYEMISLADVNLGRAFRTQNYTYWKYAFLFMGRGVAAAKKQT
YKKFSRIITPTIYTKLSKSRKNRNLKEQVTQKMSLKLHTSPKELEKQLVFYEELFKDNEQAYDLKQYFKLTDDEVKLFRS
RKIPASVEKKRLTKLRKEQELEEKEIQKQKIIDAKKVELKEESKNKKEIKVKTKKDTVEDSSKTKSTTKSKKTSKSTPTK
KVTKTKKSSNSTTKNKTESPKNSSKTSSKTSVDDKKTSKKNNKKKSRQTTLFDF
>Mature_534_residues
MKWVEKYAPKKLGDVLGNAKAKAQIEVWANKWSKGVPQKPLLLMGPPGIGKTTIAHLVGKEYFSETIEVNASDKRSYDII
KSSIGEAAQTRSLFHSGYKLLIMDEVDGISGRDDSGGARAVNETIKNSKQPIILMANDAYSKRLTSIKPKCQGIKFTKVH
TNSINAQLKRICAREDIEYDSEALYTLSKESNGDLRSAITSLEAIVDNDKKITKDSLSVIAKKDGEQNIFDTVVAVLKSK
NPEHVAEAMRVNTQPPFLIELIAENIPREYERTNEIVKAYEMISLADVNLGRAFRTQNYTYWKYAFLFMGRGVAAAKKQT
YKKFSRIITPTIYTKLSKSRKNRNLKEQVTQKMSLKLHTSPKELEKQLVFYEELFKDNEQAYDLKQYFKLTDDEVKLFRS
RKIPASVEKKRLTKLRKEQELEEKEIQKQKIIDAKKVELKEESKNKKEIKVKTKKDTVEDSSKTKSTTKSKKTSKSTPTK
KVTKTKKSSNSTTKNKTESPKNSSKTSSKTSVDDKKTSKKNNKKKSRQTTLFDF

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=270, Percent_Identity=30, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI6677723, Length=210, Percent_Identity=29.0476190476191, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI194306571, Length=208, Percent_Identity=28.8461538461538, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI194306567, Length=208, Percent_Identity=28.8461538461538, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI31563534, Length=211, Percent_Identity=29.8578199052133, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI27501458, Length=199, Percent_Identity=26.6331658291457, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI4506491, Length=295, Percent_Identity=27.7966101694915, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI31881687, Length=295, Percent_Identity=27.7966101694915, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194306569, Length=139, Percent_Identity=26.6187050359712, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17563226, Length=241, Percent_Identity=29.8755186721992, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17541988, Length=222, Percent_Identity=28.8288288288288, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17541368, Length=236, Percent_Identity=25.4237288135593, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71986063, Length=219, Percent_Identity=27.8538812785388, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17554730, Length=218, Percent_Identity=27.0642201834862, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324791, Length=226, Percent_Identity=30.9734513274336, Blast_Score=113, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323724, Length=258, Percent_Identity=26.7441860465116, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324039, Length=306, Percent_Identity=29.7385620915033, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324478, Length=226, Percent_Identity=27.8761061946903, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322528, Length=219, Percent_Identity=27.8538812785388, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17737765, Length=281, Percent_Identity=29.8932384341637, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI62472391, Length=303, Percent_Identity=27.7227722772277, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19921076, Length=252, Percent_Identity=26.1904761904762, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17647857, Length=224, Percent_Identity=26.7857142857143, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28574716, Length=225, Percent_Identity=27.1111111111111, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859927, Length=215, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_METST (Q2NH88)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447458.1
- ProteinModelPortal:   Q2NH88
- SMR:   Q2NH88
- STRING:   Q2NH88
- GeneID:   3855688
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0414
- NMPDR:   fig|339860.6.peg.397
- eggNOG:   arNOG04036
- HOGENOM:   HBG541322
- OMA:   IMPPSRW
- PhylomeDB:   Q2NH88
- ProtClustDB:   PRK04195
- BioCyc:   MSTA339860:MSP_0414-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: NA

Molecular weight: Translated: 60778; Mature: 60778

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWVEKYAPKKLGDVLGNAKAKAQIEVWANKWSKGVPQKPLLLMGPPGIGKTTIAHLVGK
CCCHHHHCHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
EYFSETIEVNASDKRSYDIIKSSIGEAAQTRSLFHSGYKLLIMDEVDGISGRDDSGGARA
HHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHH
VNETIKNSKQPIILMANDAYSKRLTSIKPKCQGIKFTKVHTNSINAQLKRICAREDIEYD
HHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCC
SEALYTLSKESNGDLRSAITSLEAIVDNDKKITKDSLSVIAKKDGEQNIFDTVVAVLKSK
CHHEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
NPEHVAEAMRVNTQPPFLIELIAENIPREYERTNEIVKAYEMISLADVNLGRAFRTQNYT
CHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
YWKYAFLFMGRGVAAAKKQTYKKFSRIITPTIYTKLSKSRKNRNLKEQVTQKMSLKLHTS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCC
PKELEKQLVFYEELFKDNEQAYDLKQYFKLTDDEVKLFRSRKIPASVEKKRLTKLRKEQE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LEEKEIQKQKIIDAKKVELKEESKNKKEIKVKTKKDTVEDSSKTKSTTKSKKTSKSTPTK
HHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCH
KVTKTKKSSNSTTKNKTESPKNSSKTSSKTSVDDKKTSKKNNKKKSRQTTLFDF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKWVEKYAPKKLGDVLGNAKAKAQIEVWANKWSKGVPQKPLLLMGPPGIGKTTIAHLVGK
CCCHHHHCHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
EYFSETIEVNASDKRSYDIIKSSIGEAAQTRSLFHSGYKLLIMDEVDGISGRDDSGGARA
HHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHH
VNETIKNSKQPIILMANDAYSKRLTSIKPKCQGIKFTKVHTNSINAQLKRICAREDIEYD
HHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCC
SEALYTLSKESNGDLRSAITSLEAIVDNDKKITKDSLSVIAKKDGEQNIFDTVVAVLKSK
CHHEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
NPEHVAEAMRVNTQPPFLIELIAENIPREYERTNEIVKAYEMISLADVNLGRAFRTQNYT
CHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
YWKYAFLFMGRGVAAAKKQTYKKFSRIITPTIYTKLSKSRKNRNLKEQVTQKMSLKLHTS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCC
PKELEKQLVFYEELFKDNEQAYDLKQYFKLTDDEVKLFRSRKIPASVEKKRLTKLRKEQE
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LEEKEIQKQKIIDAKKVELKEESKNKKEIKVKTKKDTVEDSSKTKSTTKSKKTSKSTPTK
HHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCH
KVTKTKKSSNSTTKNKTESPKNSSKTSSKTSVDDKKTSKKNNKKKSRQTTLFDF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA