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The map label for this gene is rfc

Identifier: 84489225

GI number: 84489225

Start: 474863

End: 475828

Strand: Direct

Name: rfc

Synonym: Msp_0413

Alternate gene names: 84489225

Gene position: 474863-475828 (Clockwise)

Preceding gene: 84489224

Following gene: 84489226

Centisome position: 26.87

GC content: 30.43

Gene sequence:

>966_bases
ATGAAAACACCATGGGTAGAAAAATACAGACCACAAACATTAGATGATGTAGTAGGACAAGAACAAATTGTTGGAAGGCT
AAAAAGATATGTAGAAGAAAAATCATTACCTAATATAATGTTTACGGGATTTGCAGGGGTAGGAAAAACAACATGTGCTC
TTGCATTAGCAAAAAGTTTATTAGGTGAATATTGGCAACAAAACTTCCTTGAACTCAATGCATCAGATGCAAGGGGAATA
GATACTGTAAGAAATGAAATTAAAAGCTTCTGTAAACTTAAAGCTGTAGGTGCTCCATTTAGAATAATATTCCTTGATGA
GGTGGATAACATGACAAAAGATGCTCAGCAAGCATTAAGACGTGAAATGGAAATGTATACTAAAACATCTTCATTTATAT
TATCATGTAACTATTCATCTAAAATAATAGATCCAATACAATCAAGATGTGCAATATTTAGATTTTCACCAATTAAAGCA
GCTAATATCATTAAACGTTTAAAATACATAGCATCAGAGGAAGGAATAGAAGCAGAACAATCAGCACTTGAAAATATTGT
TTACTTTACTCAAGGTGATATGAGAAAATCTATTAACATTCTTCAAGCATCAACAACCACAGAAAACACAGTAACAGAAG
AAGCAGTATATGATGTAATAAGTAGAGCAAAACCTAAAGATGTTCGTAAAATCATTAATAAAGCATTAAATCATGATTTT
ATGGAAGCACGTGATTTACTCAGGGATATTATGATTATTGAAGGAGTTAGTGGTGATGATTTAATTACACAATTCTATCA
AGAAGTAGCACAAATGACACAAGAAGAACTAATTCCAGAAGTAGAATTTATAAAATTAATGGAATATATGAGTGAATGTG
ATTATAGAATAAGAGAAGGTTCTAATCCAAGACTTCAATTAGAAGCTTTATTAAGTAAATTTTTATTAGTTAAACAGGAT
GCATAA

Upstream 100 bases:

>100_bases
ATTAAAAATCATCAACATATATAATAATTAATTAAAAAATTTTTTTTAAAATAGAAAAAAAGAACTTAATAATAAAATCA
TGAATTAGGTGAAAAATATC

Downstream 100 bases:

>100_bases
AATTGAAATGGGTAGAAAAATATGCACCTAAAAAATTAGGTGACGTACTTGGAAATGCCAAAGCAAAGGCACAGATAGAA
GTATGGGCAAATAAATGGTC

Product: replication factor C small subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKTPWVEKYRPQTLDDVVGQEQIVGRLKRYVEEKSLPNIMFTGFAGVGKTTCALALAKSLLGEYWQQNFLELNASDARGI
DTVRNEIKSFCKLKAVGAPFRIIFLDEVDNMTKDAQQALRREMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFSPIKA
ANIIKRLKYIASEEGIEAEQSALENIVYFTQGDMRKSINILQASTTTENTVTEEAVYDVISRAKPKDVRKIINKALNHDF
MEARDLLRDIMIIEGVSGDDLITQFYQEVAQMTQEELIPEVEFIKLMEYMSECDYRIREGSNPRLQLEALLSKFLLVKQD
A

Sequences:

>Translated_321_residues
MKTPWVEKYRPQTLDDVVGQEQIVGRLKRYVEEKSLPNIMFTGFAGVGKTTCALALAKSLLGEYWQQNFLELNASDARGI
DTVRNEIKSFCKLKAVGAPFRIIFLDEVDNMTKDAQQALRREMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFSPIKA
ANIIKRLKYIASEEGIEAEQSALENIVYFTQGDMRKSINILQASTTTENTVTEEAVYDVISRAKPKDVRKIINKALNHDF
MEARDLLRDIMIIEGVSGDDLITQFYQEVAQMTQEELIPEVEFIKLMEYMSECDYRIREGSNPRLQLEALLSKFLLVKQD
A
>Mature_321_residues
MKTPWVEKYRPQTLDDVVGQEQIVGRLKRYVEEKSLPNIMFTGFAGVGKTTCALALAKSLLGEYWQQNFLELNASDARGI
DTVRNEIKSFCKLKAVGAPFRIIFLDEVDNMTKDAQQALRREMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFSPIKA
ANIIKRLKYIASEEGIEAEQSALENIVYFTQGDMRKSINILQASTTTENTVTEEAVYDVISRAKPKDVRKIINKALNHDF
MEARDLLRDIMIIEGVSGDDLITQFYQEVAQMTQEELIPEVEFIKLMEYMSECDYRIREGSNPRLQLEALLSKFLLVKQD
A

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily

Homologues:

Organism=Homo sapiens, GI31563534, Length=314, Percent_Identity=41.4012738853503, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI6677723, Length=316, Percent_Identity=37.3417721518987, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI194306571, Length=314, Percent_Identity=36.9426751592357, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI194306567, Length=314, Percent_Identity=36.9426751592357, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4506491, Length=323, Percent_Identity=36.2229102167183, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI31881687, Length=323, Percent_Identity=36.2229102167183, Blast_Score=205, Evalue=5e-53,
Organism=Homo sapiens, GI28882049, Length=311, Percent_Identity=36.0128617363344, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI194306569, Length=246, Percent_Identity=36.5853658536585, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4506489, Length=346, Percent_Identity=24.8554913294798, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI108773789, Length=230, Percent_Identity=28.2608695652174, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786676, Length=230, Percent_Identity=27.3913043478261, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17541988, Length=317, Percent_Identity=41.3249211356467, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71986063, Length=315, Percent_Identity=36.5079365079365, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17554730, Length=322, Percent_Identity=34.472049689441, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17541990, Length=251, Percent_Identity=26.2948207171315, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324478, Length=316, Percent_Identity=37.3417721518987, Blast_Score=232, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6324039, Length=315, Percent_Identity=34.6031746031746, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322528, Length=330, Percent_Identity=35.7575757575758, Blast_Score=201, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6319562, Length=355, Percent_Identity=24.5070422535211, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324791, Length=256, Percent_Identity=27.734375, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17647857, Length=319, Percent_Identity=39.1849529780564, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI19921076, Length=324, Percent_Identity=34.8765432098765, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI18859927, Length=328, Percent_Identity=35.9756097560976, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI19921136, Length=355, Percent_Identity=23.943661971831, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCS_METST (Q2NH89)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447457.1
- ProteinModelPortal:   Q2NH89
- SMR:   Q2NH89
- STRING:   Q2NH89
- GeneID:   3855687
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0413
- NMPDR:   fig|339860.6.peg.396
- eggNOG:   arNOG04037
- HOGENOM:   HBG541599
- OMA:   CDFRIVE
- PhylomeDB:   Q2NH89
- ProtClustDB:   PRK00440
- BioCyc:   MSTA339860:MSP_0413-MONOMER
- HAMAP:   MF_01509
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C; SSF48019 Pol_clamp_load_C

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 36752; Mature: 36752

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPWVEKYRPQTLDDVVGQEQIVGRLKRYVEEKSLPNIMFTGFAGVGKTTCALALAKSL
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
LGEYWQQNFLELNASDARGIDTVRNEIKSFCKLKAVGAPFRIIFLDEVDNMTKDAQQALR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
REMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFSPIKAANIIKRLKYIASEEGIEAEQ
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
SALENIVYFTQGDMRKSINILQASTTTENTVTEEAVYDVISRAKPKDVRKIINKALNHDF
HHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
MEARDLLRDIMIIEGVSGDDLITQFYQEVAQMTQEELIPEVEFIKLMEYMSECDYRIREG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCC
SNPRLQLEALLSKFLLVKQDA
CCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTPWVEKYRPQTLDDVVGQEQIVGRLKRYVEEKSLPNIMFTGFAGVGKTTCALALAKSL
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
LGEYWQQNFLELNASDARGIDTVRNEIKSFCKLKAVGAPFRIIFLDEVDNMTKDAQQALR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHH
REMEMYTKTSSFILSCNYSSKIIDPIQSRCAIFRFSPIKAANIIKRLKYIASEEGIEAEQ
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
SALENIVYFTQGDMRKSINILQASTTTENTVTEEAVYDVISRAKPKDVRKIINKALNHDF
HHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHH
MEARDLLRDIMIIEGVSGDDLITQFYQEVAQMTQEELIPEVEFIKLMEYMSECDYRIREG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCC
SNPRLQLEALLSKFLLVKQDA
CCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA