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The map label for this gene is radB

Identifier: 84489183

GI number: 84489183

Start: 436433

End: 437131

Strand: Direct

Name: radB

Synonym: Msp_0369

Alternate gene names: 84489183

Gene position: 436433-437131 (Clockwise)

Preceding gene: 84489182

Following gene: 84489184

Centisome position: 24.69

GC content: 28.33

Gene sequence:

>699_bases
TTGAAGACACTTTCAGACATTTCAGAATCTGTTTTAATTCCTACTAATTCTTCACTGGATAAACTTCTTGGTGGAGGTAT
AGAAAAAGGATGTATTACTCAGTTTTATGGACCTCCTGGTTCTGGAAAAACTAATATTGCATTGAAAATATTATATGAAG
CTACAAAAAATGGATCTAAGGCAATATATATGGATACTGAAGGTGGATTATCTTTAGAGAGAATACAACAAATAGCGGGA
ACTGATTTTGGTTCTATATCTAAGAATATTTATATTTTAGAGCCAAAATCCTTTGATGAACAAATATTGGATATTCAAAA
TATTGAAGACATATTAAAAAAAGACAAATCTATTGATATGTTAATAATAGATTCAATAGTAGCATTGTATAGGGTAGAGG
ATGGAGATCCTTCAGAAATAAATAAGAGATTGGGTCGGCTAATGGCAAAATTATTGAGATTAAGTAGGGAATATAACGTA
GCAATTGTTATTACTAATCAAATATATTCTCCATTTGATTCTGATGATTTAATAATAGAACCTATTGGTGGTACAGTTTT
AAAATATTGGAGTAAAATAATAATTGAAATAGAAAAAAGTGTAGATTCTCTAAAAAGAACAGCTACATTACAAAGACATA
AAACTAAAGCACCAGGTCAATCAATAAAATTTGAGATTATTGATAGAGGAATTATATAA

Upstream 100 bases:

>100_bases
TAAAAAGTAGATACTCTTTTTTTATAAAACTATGATTTAACTATTTATATAATAACTTATAGAATTATTACCAAATGAAT
AAGGTTATGGAGATATTATT

Downstream 100 bases:

>100_bases
TTAAGAAATAGGGAGTAGAATTATGGTTTTTAAAAAGAAGGAAAAAAGAGTTCCAAGTGGATTTAACACAATAAATGAAT
TCTTTGATTACTTATATAAA

Product: DNA repair and recombination protein RadB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MKTLSDISESVLIPTNSSLDKLLGGGIEKGCITQFYGPPGSGKTNIALKILYEATKNGSKAIYMDTEGGLSLERIQQIAG
TDFGSISKNIYILEPKSFDEQILDIQNIEDILKKDKSIDMLIIDSIVALYRVEDGDPSEINKRLGRLMAKLLRLSREYNV
AIVITNQIYSPFDSDDLIIEPIGGTVLKYWSKIIIEIEKSVDSLKRTATLQRHKTKAPGQSIKFEIIDRGII

Sequences:

>Translated_232_residues
MKTLSDISESVLIPTNSSLDKLLGGGIEKGCITQFYGPPGSGKTNIALKILYEATKNGSKAIYMDTEGGLSLERIQQIAG
TDFGSISKNIYILEPKSFDEQILDIQNIEDILKKDKSIDMLIIDSIVALYRVEDGDPSEINKRLGRLMAKLLRLSREYNV
AIVITNQIYSPFDSDDLIIEPIGGTVLKYWSKIIIEIEKSVDSLKRTATLQRHKTKAPGQSIKFEIIDRGII
>Mature_232_residues
MKTLSDISESVLIPTNSSLDKLLGGGIEKGCITQFYGPPGSGKTNIALKILYEATKNGSKAIYMDTEGGLSLERIQQIAG
TDFGSISKNIYILEPKSFDEQILDIQNIEDILKKDKSIDMLIIDSIVALYRVEDGDPSEINKRLGRLMAKLLRLSREYNV
AIVITNQIYSPFDSDDLIIEPIGGTVLKYWSKIIIEIEKSVDSLKRTATLQRHKTKAPGQSIKFEIIDRGII

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily

Homologues:

Organism=Homo sapiens, GI19924133, Length=209, Percent_Identity=32.5358851674641, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI23238219, Length=209, Percent_Identity=30.1435406698565, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI46255039, Length=185, Percent_Identity=31.3513513513514, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI19924117, Length=185, Percent_Identity=31.3513513513514, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI10835029, Length=185, Percent_Identity=31.3513513513514, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI256017143, Length=201, Percent_Identity=29.8507462686567, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI256017141, Length=201, Percent_Identity=29.8507462686567, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI17402896, Length=257, Percent_Identity=22.568093385214, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI256017145, Length=163, Percent_Identity=33.1288343558282, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1789051, Length=230, Percent_Identity=26.0869565217391, Blast_Score=63, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71997301, Length=210, Percent_Identity=30.4761904761905, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71997295, Length=210, Percent_Identity=30.4761904761905, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321027, Length=210, Percent_Identity=31.9047619047619, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320207, Length=170, Percent_Identity=30, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320942, Length=209, Percent_Identity=28.2296650717703, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864108, Length=196, Percent_Identity=32.6530612244898, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651285, Length=196, Percent_Identity=32.6530612244898, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RADB_METST (Q2NHD1)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447415.1
- HSSP:   O73948
- ProteinModelPortal:   Q2NHD1
- SMR:   Q2NHD1
- STRING:   Q2NHD1
- GeneID:   3855602
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0369
- NMPDR:   fig|339860.6.peg.353
- eggNOG:   arNOG05079
- HOGENOM:   HBG413235
- OMA:   ITQIYGP
- PhylomeDB:   Q2NHD1
- ProtClustDB:   PRK09361
- BioCyc:   MSTA339860:MSP_0369-MONOMER
- HAMAP:   MF_00350
- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR013765
- InterPro:   IPR020588
- PANTHER:   PTHR22942:SF9
- PIRSF:   PIRSF003336
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02237

Pfam domain/function: PF08423 Rad51

EC number: NA

Molecular weight: Translated: 25795; Mature: 25795

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLSDISESVLIPTNSSLDKLLGGGIEKGCITQFYGPPGSGKTNIALKILYEATKNGSK
CCCHHHCCCCEEECCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCE
AIYMDTEGGLSLERIQQIAGTDFGSISKNIYILEPKSFDEQILDIQNIEDILKKDKSIDM
EEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCH
LIIDSIVALYRVEDGDPSEINKRLGRLMAKLLRLSREYNVAIVITNQIYSPFDSDDLIIE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEE
PIGGTVLKYWSKIIIEIEKSVDSLKRTATLQRHKTKAPGQSIKFEIIDRGII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKTLSDISESVLIPTNSSLDKLLGGGIEKGCITQFYGPPGSGKTNIALKILYEATKNGSK
CCCHHHCCCCEEECCCCCHHHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCE
AIYMDTEGGLSLERIQQIAGTDFGSISKNIYILEPKSFDEQILDIQNIEDILKKDKSIDM
EEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCH
LIIDSIVALYRVEDGDPSEINKRLGRLMAKLLRLSREYNVAIVITNQIYSPFDSDDLIIE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEE
PIGGTVLKYWSKIIIEIEKSVDSLKRTATLQRHKTKAPGQSIKFEIIDRGII
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA