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The map label for this gene is mtaC1

Identifier: 84489017

GI number: 84489017

Start: 234176

End: 235009

Strand: Direct

Name: mtaC1

Synonym: Msp_0189

Alternate gene names: 84489017

Gene position: 234176-235009 (Clockwise)

Preceding gene: 84489016

Following gene: 84489018

Centisome position: 13.25

GC content: 36.33

Gene sequence:

>834_bases
ATGGATAGTCCGTTAGAAAAATATTATGGAAAATTAACAGATTATGATACAATTGCTATAAGATACAATGTTAAAATTGA
AGGTCCTGCTCTTAAACCTGAAGATGATCCTGAAGTAATGGAAATATTACCTAAAGAAGAAGGAATAAAAAGAACAGTGG
CTTTAGCTGTATTATATGGTGACAAAGACGAATGTGATGCACAAGTAGAAAAAGCATTAGATGCTGGTGAAGAACCAATA
GACCTTATTAACAACGCTTTAATGAAAGGTATGGATGGTGTAAGTGCATTATATACTAAAGGTGAATTCTTCCTTCCTGA
TTTAATGCTTGCAGGAGATGCAATGATGTCTGGAGTAGCACTTTGTGAAGCAAAACTTGGACACAAAGCAGATTCCAAAG
CAACAGTATGTTGTTGTGCAGTAGAAGGAGACCCTCACGATATTGGTAAAAACTTAATTGTTATGTTCTTAAATGCAAAT
GGATATGAAGCAGTAGATCTTGGTCGTGATGTACCTAACACTAAAGTAGTAGAAGCAGTAAAAGAAAACAAACCAGTACT
TGTAACTGCAACAGCATTAATGACAACAACCATGACAGCATTTGGTAAAATCATAGCATTAATGCAAGAAGCAGGACTTG
ACACACCATTTGGTTGTGGAGGAGGAGCAGTACGTCGTGACTTTGTAGAAGAAAGTCCACAAACATTCTATGGTGTAGAA
GCATACCACGTACCAAAAATAGCAGATGCTATTGTAGATGATGGTAAAACATGGGAAGACATCAGAAAAGAATATGCTGA
TATTGTTGGAGAATATGTAGCAGCATACTCCTAG

Upstream 100 bases:

>100_bases
TAAATTCATAAAAACATGTATAAAACGTTATGGAAGAAAAGTAAAAGAACATGATCCATCACAATATGAATTATAAATAA
TTGAAAGGAAGTGTGTAAAA

Downstream 100 bases:

>100_bases
AACTTGTCTACAAATTAGAAGATATAAAACTATTTTCTTTTCTTCTAACCTCCCTTTTTTTATAATTTAATTTAAATTAA
ACTTATTTTTTAAGATATTT

Product: MtaC1

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MDSPLEKYYGKLTDYDTIAIRYNVKIEGPALKPEDDPEVMEILPKEEGIKRTVALAVLYGDKDECDAQVEKALDAGEEPI
DLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVALCEAKLGHKADSKATVCCCAVEGDPHDIGKNLIVMFLNAN
GYEAVDLGRDVPNTKVVEAVKENKPVLVTATALMTTTMTAFGKIIALMQEAGLDTPFGCGGGAVRRDFVEESPQTFYGVE
AYHVPKIADAIVDDGKTWEDIRKEYADIVGEYVAAYS

Sequences:

>Translated_277_residues
MDSPLEKYYGKLTDYDTIAIRYNVKIEGPALKPEDDPEVMEILPKEEGIKRTVALAVLYGDKDECDAQVEKALDAGEEPI
DLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVALCEAKLGHKADSKATVCCCAVEGDPHDIGKNLIVMFLNAN
GYEAVDLGRDVPNTKVVEAVKENKPVLVTATALMTTTMTAFGKIIALMQEAGLDTPFGCGGGAVRRDFVEESPQTFYGVE
AYHVPKIADAIVDDGKTWEDIRKEYADIVGEYVAAYS
>Mature_277_residues
MDSPLEKYYGKLTDYDTIAIRYNVKIEGPALKPEDDPEVMEILPKEEGIKRTVALAVLYGDKDECDAQVEKALDAGEEPI
DLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVALCEAKLGHKADSKATVCCCAVEGDPHDIGKNLIVMFLNAN
GYEAVDLGRDVPNTKVVEAVKENKPVLVTATALMTTTMTAFGKIIALMQEAGLDTPFGCGGGAVRRDFVEESPQTFYGVE
AYHVPKIADAIVDDGKTWEDIRKEYADIVGEYVAAYS

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=214, Percent_Identity=23.3644859813084, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790450, Length=181, Percent_Identity=27.6243093922652, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535405, Length=222, Percent_Identity=26.1261261261261, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 30009; Mature: 30009

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPLEKYYGKLTDYDTIAIRYNVKIEGPALKPEDDPEVMEILPKEEGIKRTVALAVLYG
CCCCHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCCHHEEEEEEEEEC
DKDECDAQVEKALDAGEEPIDLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCEECCHHHHHHHHHHHHHH
LCEAKLGHKADSKATVCCCAVEGDPHDIGKNLIVMFLNANGYEAVDLGRDVPNTKVVEAV
HHHHHHCCCCCCCCEEEEEEECCCHHHCCCEEEEEEECCCCCEEHHCCCCCCCHHHHHHH
KENKPVLVTATALMTTTMTAFGKIIALMQEAGLDTPFGCGGGAVRRDFVEESPQTFYGVE
HCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEECCC
AYHVPKIADAIVDDGKTWEDIRKEYADIVGEYVAAYS
EECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSPLEKYYGKLTDYDTIAIRYNVKIEGPALKPEDDPEVMEILPKEEGIKRTVALAVLYG
CCCCHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHCCCCCCHHEEEEEEEEEC
DKDECDAQVEKALDAGEEPIDLINNALMKGMDGVSALYTKGEFFLPDLMLAGDAMMSGVA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCEECCHHHHHHHHHHHHHH
LCEAKLGHKADSKATVCCCAVEGDPHDIGKNLIVMFLNANGYEAVDLGRDVPNTKVVEAV
HHHHHHCCCCCCCCEEEEEEECCCHHHCCCEEEEEEECCCCCEEHHCCCCCCCHHHHHHH
KENKPVLVTATALMTTTMTAFGKIIALMQEAGLDTPFGCGGGAVRRDFVEESPQTFYGVE
HCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEECCC
AYHVPKIADAIVDDGKTWEDIRKEYADIVGEYVAAYS
EECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]