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The map label for this gene is dfrA [H]

Identifier: 83721102

GI number: 83721102

Start: 1883037

End: 1883696

Strand: Reverse

Name: dfrA [H]

Synonym: BTH_I1674

Alternate gene names: 83721102

Gene position: 1883696-1883037 (Counterclockwise)

Preceding gene: 83719894

Following gene: 83719689

Centisome position: 49.45

GC content: 70.0

Gene sequence:

>660_bases
GTGTGCGCGAAGCGTCGGCGCAGGGCGCATCAAGCGTCGATCGCCGCAAGGCGCGCGAGAACGCGTATTCCGAACCGGCG
CGAAGCACGACGCGCGCGGGCGCGTGCGGCGCGATCCGGTAGAATCGTGCCCTTCCGGCATCTTTTTCTTCCCGTCATGA
CGACGCTGACCCTGATCGTCGCGCGCGCCCGCAATGGCGTGATCGGCCGCGACAACCGGCTTCCGTGGAAACTCCCCGAG
GACCTCGCGTTCTTCAAGCGCACGACGATGGGCGCGCCGATCGTGATGGGCCGCAAGACGCACGAGTCGATCGGCCGGCC
GCTGCCCGGCCGCCGCAACATCGTCGTCACGCGCGACGCCGCGCGACGCTTCGACGGCTGCGACACGGCCACGTCGCTCG
GCGACGCGCTCGCGCTCGCCGAGCGCGACGGCGCGGCCGAAGCGTTCCTGATCGGCGGCGCGCAACTGTACGCGGAGGGC
CTGCGCCATGCGGACAAGCTGATCGTCACCGAGATCGATCAGGATTTCGAAGGCGACGCGTCGTTTCCCGCGCCCGATCC
CGCGCAGTGGGAAGCCGTGTCGCGCGATGCGCATCGCGCGGCCCCGCCGAACGATTTCGCATACGCGTTCGTCGTCTACC
GTCGCAAGCGCGCCGCCTGA

Upstream 100 bases:

>100_bases
GATGGTGTCGCACGGCCTGCGCGAGTTGTCATGGGGCACGCATCGCACGGGCGCGAGCGATCCGGACGACGAAGCCGAGC
CCACGTGAAGTGCGGGCGGC

Downstream 100 bases:

>100_bases
CGGACGCGAGCCCGCGCGCGATATCGCGGGCTCCCGCGCGACGACACAAAAAAGGCGCCGCTCGCCGCGGCGCCTCTTGC
TTTTGCGCGATCGAAACGAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MCAKRRRRAHQASIAARRARTRIPNRREARRARARAARSGRIVPFRHLFLPVMTTLTLIVARARNGVIGRDNRLPWKLPE
DLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDAARRFDGCDTATSLGDALALAERDGAAEAFLIGGAQLYAEG
LRHADKLIVTEIDQDFEGDASFPAPDPAQWEAVSRDAHRAAPPNDFAYAFVVYRRKRAA

Sequences:

>Translated_219_residues
MCAKRRRRAHQASIAARRARTRIPNRREARRARARAARSGRIVPFRHLFLPVMTTLTLIVARARNGVIGRDNRLPWKLPE
DLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDAARRFDGCDTATSLGDALALAERDGAAEAFLIGGAQLYAEG
LRHADKLIVTEIDQDFEGDASFPAPDPAQWEAVSRDAHRAAPPNDFAYAFVVYRRKRAA
>Mature_219_residues
MCAKRRRRAHQASIAARRARTRIPNRREARRARARAARSGRIVPFRHLFLPVMTTLTLIVARARNGVIGRDNRLPWKLPE
DLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDAARRFDGCDTATSLGDALALAERDGAAEAFLIGGAQLYAEG
LRHADKLIVTEIDQDFEGDASFPAPDPAQWEAVSRDAHRAAPPNDFAYAFVVYRRKRAA

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=185, Percent_Identity=32.972972972973, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI28827793, Length=156, Percent_Identity=35.2564102564103, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786233, Length=161, Percent_Identity=41.6149068322981, Blast_Score=130, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17505899, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI116008026, Length=150, Percent_Identity=34.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24647458, Length=150, Percent_Identity=34.6666666666667, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 24446; Mature: 24446

Theoretical pI: Translated: 11.92; Mature: 11.92

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAKRRRRAHQASIAARRARTRIPNRREARRARARAARSGRIVPFRHLFLPVMTTLTLIV
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARARNGVIGRDNRLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDA
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCEEEECHH
ARRFDGCDTATSLGDALALAERDGAAEAFLIGGAQLYAEGLRHADKLIVTEIDQDFEGDA
HHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCC
SFPAPDPAQWEAVSRDAHRAAPPNDFAYAFVVYRRKRAA
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MCAKRRRRAHQASIAARRARTRIPNRREARRARARAARSGRIVPFRHLFLPVMTTLTLIV
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ARARNGVIGRDNRLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDA
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCEEEECHH
ARRFDGCDTATSLGDALALAERDGAAEAFLIGGAQLYAEGLRHADKLIVTEIDQDFEGDA
HHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCC
SFPAPDPAQWEAVSRDAHRAAPPNDFAYAFVVYRRKRAA
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]