Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 83720577

GI number: 83720577

Start: 1172517

End: 1173911

Strand: Direct

Name: gatA [H]

Synonym: BTH_I1032

Alternate gene names: 83720577

Gene position: 1172517-1173911 (Clockwise)

Preceding gene: 83719480

Following gene: 83721269

Centisome position: 30.78

GC content: 73.48

Gene sequence:

>1395_bases
ATGTCCGAGTTGCTCGACCTGAGCGCTTGCTCGCTCGCCGAACGGTTCAAGAACCGCAGCGTGACGCCCGCCGACTACGC
GGACGCGCTGCTCGATCACATCGCCCGGTGGGAGCCGCATCTGAATGCGCTGTGCCGCTTCGATCCGGCGCAGGTGCGCG
AGCAGGCGCTCGCGTCGACGCGCCGCTGGGCCGCGGGTGTGCCGCTCAGCGAGATCGACGGCGTGCCCGTGACGATCAAG
GAGCTGATCGCGACACAGGGCGATCGGGTGGCGCAGGGCTCGGCCGCGGCCGCCGACGCGCCGCCCGCGGTTGCCGACGC
GCCCGCTGCGGCGCGGCTGCGCGAGGCGGGCGCGGTCGTGATCGGCAAGACGACGGTGCCCGATTACGCGATGCTGTCGT
CGGGGCTGTCGAGCCTGTACGGCATCACGCGCAACCCGTGGCGGCTCGATCTGAATCCGGGCGGCTCGAGCAGCGGCGCG
GCCGCGGCGGCGGCCGCAGGCTACGGGCCGCTGCACGTGGGCACCGACATCGGCGGGTCGATCCGGCTGCCGGCCGGCTG
GTGCGGCATCGTCGGCTTCAAGCCGTCGAACGGGCGGATTCCGATCGATCCGTACTACACGGGCCGCTGCGCGGGGCCGA
TGACGCGCACGATCGACGATGCCGCGCTGCTGATGCGCTTCCTGTCGCGGCCGGACTGGCGCGACGCGACGAGCCTGCCG
CCCGAGGCGATCGACTGGTCGATTACGCCCGCCGACGTGAAGGGAATGCGGATCGGCCTGATGCTCGACGCCGGCTGCGG
GATCGAGCCCGAGGCGGAGATCCTCGCCGCGGTGGACGCGGCGGCCGAGCGCGTTGCCGCGCACGGCGCGCGGATCGTCG
CGGCGCCGCCCGTGCTGTCGCGCGCGATGCTCGACGGGCTCGACCGTTTCTGGCAGGCGCGGCTGTGGGCGGATCTCGAG
CGGCTGCCGGACGTGGCGCGCGAGCGCATCCTGCCGTACATCGTCGCGTGGGCTGGGCAGGGCGCGACGGTGTCCGGCGT
CGATGCGGTGCGCGGCTTCGGCGCGACGTTCGAGATGCGCGCGGCGGCTGCGCGGCTGTTCCAGTCGGTCGACGCGGTGC
TGTCGCCGACCAACGTCGTCACCGGCTTCCCGGCCGAGTGGGCGGGGCCGACCGACGATCCGGCGCGGCCGTTCGAGCAC
ATCGCGTTCACGGTTCCGTGGAACATGGGCGAGCAGCCGGCGTTGTCGATCCACTGCGGCTTCGCGCGCAATGGGATGCC
GATCGGCCTGCAGATCGTCGGGCCGCGCTTTGCCGACCGGCTCGTGCTGCAGCTCGGCCGCGCGTACGAGGCGTGGCGAG
GCGAGATGCCGCGCTGGCCGGCGGCGCCGCGCTGA

Upstream 100 bases:

>100_bases
ATGCATCGCCATGATTGCGTCGCTATCGTGAGTTCCATCGACGACGGGCGGTCACGGGCGAAGGCCTGACCCCCGTCTCC
AACCTTGCGAGGCCGATACG

Downstream 100 bases:

>100_bases
CGCGGCTGAATGCGCGGCGCGCCGCGTGCCGCCGTGCCGCCGTGCGTGTGCAGCCGGTTCCTTCGCCGCCTTTTCGTTTG
CTTGCCTGTTCATCGTGATA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSELLDLSACSLAERFKNRSVTPADYADALLDHIARWEPHLNALCRFDPAQVREQALASTRRWAAGVPLSEIDGVPVTIK
ELIATQGDRVAQGSAAAADAPPAVADAPAAARLREAGAVVIGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGA
AAAAAAGYGPLHVGTDIGGSIRLPAGWCGIVGFKPSNGRIPIDPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
PEAIDWSITPADVKGMRIGLMLDAGCGIEPEAEILAAVDAAAERVAAHGARIVAAPPVLSRAMLDGLDRFWQARLWADLE
RLPDVARERILPYIVAWAGQGATVSGVDAVRGFGATFEMRAAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEH
IAFTVPWNMGEQPALSIHCGFARNGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAAPR

Sequences:

>Translated_464_residues
MSELLDLSACSLAERFKNRSVTPADYADALLDHIARWEPHLNALCRFDPAQVREQALASTRRWAAGVPLSEIDGVPVTIK
ELIATQGDRVAQGSAAAADAPPAVADAPAAARLREAGAVVIGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGA
AAAAAAGYGPLHVGTDIGGSIRLPAGWCGIVGFKPSNGRIPIDPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
PEAIDWSITPADVKGMRIGLMLDAGCGIEPEAEILAAVDAAAERVAAHGARIVAAPPVLSRAMLDGLDRFWQARLWADLE
RLPDVARERILPYIVAWAGQGATVSGVDAVRGFGATFEMRAAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEH
IAFTVPWNMGEQPALSIHCGFARNGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAAPR
>Mature_463_residues
SELLDLSACSLAERFKNRSVTPADYADALLDHIARWEPHLNALCRFDPAQVREQALASTRRWAAGVPLSEIDGVPVTIKE
LIATQGDRVAQGSAAAADAPPAVADAPAAARLREAGAVVIGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGAA
AAAAAGYGPLHVGTDIGGSIRLPAGWCGIVGFKPSNGRIPIDPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLPP
EAIDWSITPADVKGMRIGLMLDAGCGIEPEAEILAAVDAAAERVAAHGARIVAAPPVLSRAMLDGLDRFWQARLWADLER
LPDVARERILPYIVAWAGQGATVSGVDAVRGFGATFEMRAAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEHI
AFTVPWNMGEQPALSIHCGFARNGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAAPR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=26.7465069860279, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI195972892, Length=496, Percent_Identity=25.6048387096774, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI166795287, Length=128, Percent_Identity=42.1875, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=488, Percent_Identity=24.7950819672131, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=205, Percent_Identity=34.1463414634146, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=211, Percent_Identity=28.9099526066351, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=457, Percent_Identity=24.2888402625821, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=434, Percent_Identity=24.1935483870968, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=233, Percent_Identity=27.4678111587983, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=132, Percent_Identity=34.0909090909091, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=448, Percent_Identity=23.4375, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320448, Length=434, Percent_Identity=23.963133640553, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=25, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=28.0876494023904, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356731, Length=477, Percent_Identity=27.0440251572327, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648435, Length=492, Percent_Identity=25.8130081300813, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648437, Length=492, Percent_Identity=25.8130081300813, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45550774, Length=492, Percent_Identity=25.8130081300813, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078093, Length=516, Percent_Identity=25.3875968992248, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=24.1176470588235, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24648441, Length=425, Percent_Identity=26.8235294117647, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648439, Length=425, Percent_Identity=26.8235294117647, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24652985, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19922090, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24652981, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24652983, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49334; Mature: 49202

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLDLSACSLAERFKNRSVTPADYADALLDHIARWEPHLNALCRFDPAQVREQALAST
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
RRWAAGVPLSEIDGVPVTIKELIATQGDRVAQGSAAAADAPPAVADAPAAARLREAGAVV
HHHHCCCCHHHCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEE
IGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGAAAAAAAGYGPLHVGTDIGGS
EECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEECCCCCCE
IRLPAGWCGIVGFKPSNGRIPIDPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
EECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PEAIDWSITPADVKGMRIGLMLDAGCGIEPEAEILAAVDAAAERVAAHGARIVAAPPVLS
CHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHH
RAMLDGLDRFWQARLWADLERLPDVARERILPYIVAWAGQGATVSGVDAVRGFGATFEMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEECCHHHHHCCCCCHHHH
AAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEHIAFTVPWNMGEQPALSIHCG
HHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCEEEEEEC
FARNGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAAPR
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SELLDLSACSLAERFKNRSVTPADYADALLDHIARWEPHLNALCRFDPAQVREQALAST
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHH
RRWAAGVPLSEIDGVPVTIKELIATQGDRVAQGSAAAADAPPAVADAPAAARLREAGAVV
HHHHCCCCHHHCCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEE
IGKTTVPDYAMLSSGLSSLYGITRNPWRLDLNPGGSSSGAAAAAAAGYGPLHVGTDIGGS
EECCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEECCCCCCE
IRLPAGWCGIVGFKPSNGRIPIDPYYTGRCAGPMTRTIDDAALLMRFLSRPDWRDATSLP
EECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PEAIDWSITPADVKGMRIGLMLDAGCGIEPEAEILAAVDAAAERVAAHGARIVAAPPVLS
CHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHH
RAMLDGLDRFWQARLWADLERLPDVARERILPYIVAWAGQGATVSGVDAVRGFGATFEMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEECCHHHHHCCCCCHHHH
AAAARLFQSVDAVLSPTNVVTGFPAEWAGPTDDPARPFEHIAFTVPWNMGEQPALSIHCG
HHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCEEEEEEC
FARNGMPIGLQIVGPRFADRLVLQLGRAYEAWRGEMPRWPAAPR
CCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA