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The map label for this gene is citA [H]

Identifier: 83721269

GI number: 83721269

Start: 1174049

End: 1175470

Strand: Direct

Name: citA [H]

Synonym: BTH_I1033

Alternate gene names: 83721269

Gene position: 1174049-1175470 (Clockwise)

Preceding gene: 83720577

Following gene: 83719261

Centisome position: 30.82

GC content: 69.69

Gene sequence:

>1422_bases
TTGCGCAGCCTGTACGGTTTGTACGGTTTGCGCCGCGCCCGCGGCCGGTTTCGTCGTCGCTTCGATCAAGGACGCTTCAT
GGAAGACCTGCTTGAATCCGCTTGTCCACCGGCGCCCCGGCATCCGGCCGGCGCAGCCGACGCGGCCGGCACGGCTGGGT
CGCGCGCCGCAGTCCGCTGCAACGTCGCGGCGGTTGCGCTCGGCAACGCGGTGGAGTTCTTCGACTTCGGCGCTTACGCG
ACTTTCGCGGTGATGATCGGCCACACGTTTTTTCCGGCGAAGAGCCCGTTCGTGAGCCTGCTGCTTGCGGTGTCCGTGTT
CGGGCTCGGCTTCGTCGTGCGTCCGCTCGGCGCGCTCGTGATCGGCGCATATGCGGACCGGGCGGGCCGCAAGCCCGCGA
TGATGCTGACGCTCGTGATGATGGCGGTCGGCACGGGGGCGATCGCCGTGCTGCCGGGATACGAAACGATCGGCGTCGCG
GCGCCGATCCTGCTCGTCGTCACGCGGCTGATCCAGGGGCTCGCGTGGGGCGGCGAGGCGGGGCCCGCGACGACCTACAT
TCTCGAAGCCGCTCCCCCCGAGCGGCGCGGCGCGTACGCGTGCTGGCAGGTCGCGACGCAGGGTTTCGCGGGGCTCGCGG
CGGGGCTCGCGGGCTACGCGCTCACGCTTGCATTGCCCGAAGCCGACCTGTACGCGTGGGGCTGGCGCGTGCCGTTCGCG
CTCGGCCTGCTGGTGCTGCCGATCGGCGTCTACATCCGCCGCCGGCTGTCCGACACGATCGACGCGCACCGTGCATACGG
CTCGACGCGCGCCATCCTGCACGAGCTGAACGCGCGGCATCGGCGGCCGATCGCGGTCGGCCTGATGATCCTGCTCGGCA
GCACGATCACGCAGTATTTCCTCAATTACATGACGACGTTTGCGCTGACCGAGCTGCATCTGCCGGGCGGCGTCGCGATG
CTCGCGACGTTCGCGACGGGCGCCGCGCTTGCCGTGGGCTCGCTCGCGGGCGGCAGCCTGTCGGATCGAGTCGGACGCCG
GGCGGTCCTGATCTGGCCGCGCGTGCTGTTGCTGCTGCTGATCTTCCCGGCGTTGCAATTGATCGTTTCGCGTCCGACGC
CCGCAGTGTTTCTCGCCACGCTGACCGTGCTGTCCGGCCTGCACGGCATGAGCGGCGCGGCGCTCATCGTGCTGATCGCC
GAGAGCTTCCCGCAACGCGTGCGCTCGACCGGGTTCTCGATCGTCTATGCCGTCGCGGTGTCGTTGTTCGGCGGCACCGC
GCAGAGCATCGTCACCTGGCTCATCGGCACCACCGGCAATCCGATGGCGCCCGCCGGCTATCTGCTCGTCGCGAACGCGA
TCTGCATCGCGGCCGGGTGGCTTGCCGTCGAGACCTGGCCGAGCGGGCGCAACACGCGTTAG

Upstream 100 bases:

>100_bases
CGCCGTGCGTGTGCAGCCGGTTCCTTCGCCGCCTTTTCGTTTGCTTGCCTGTTCATCGTGATACCGCCTGCGGTTCCGCC
GTGCGTGACGCTCGCGCGGC

Downstream 100 bases:

>100_bases
CGCGGCGTCCACACACCGCGTCGGCTTCGATCGTTGTTGTGCCGGCGGTAGCCTGAGGGAGAGGGCCGCAAGGCGGCCGG
CTGGACGACGCGTCTGCTGG

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MRSLYGLYGLRRARGRFRRRFDQGRFMEDLLESACPPAPRHPAGAADAAGTAGSRAAVRCNVAAVALGNAVEFFDFGAYA
TFAVMIGHTFFPAKSPFVSLLLAVSVFGLGFVVRPLGALVIGAYADRAGRKPAMMLTLVMMAVGTGAIAVLPGYETIGVA
APILLVVTRLIQGLAWGGEAGPATTYILEAAPPERRGAYACWQVATQGFAGLAAGLAGYALTLALPEADLYAWGWRVPFA
LGLLVLPIGVYIRRRLSDTIDAHRAYGSTRAILHELNARHRRPIAVGLMILLGSTITQYFLNYMTTFALTELHLPGGVAM
LATFATGAALAVGSLAGGSLSDRVGRRAVLIWPRVLLLLLIFPALQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIA
ESFPQRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAPAGYLLVANAICIAAGWLAVETWPSGRNTR

Sequences:

>Translated_473_residues
MRSLYGLYGLRRARGRFRRRFDQGRFMEDLLESACPPAPRHPAGAADAAGTAGSRAAVRCNVAAVALGNAVEFFDFGAYA
TFAVMIGHTFFPAKSPFVSLLLAVSVFGLGFVVRPLGALVIGAYADRAGRKPAMMLTLVMMAVGTGAIAVLPGYETIGVA
APILLVVTRLIQGLAWGGEAGPATTYILEAAPPERRGAYACWQVATQGFAGLAAGLAGYALTLALPEADLYAWGWRVPFA
LGLLVLPIGVYIRRRLSDTIDAHRAYGSTRAILHELNARHRRPIAVGLMILLGSTITQYFLNYMTTFALTELHLPGGVAM
LATFATGAALAVGSLAGGSLSDRVGRRAVLIWPRVLLLLLIFPALQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIA
ESFPQRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAPAGYLLVANAICIAAGWLAVETWPSGRNTR
>Mature_473_residues
MRSLYGLYGLRRARGRFRRRFDQGRFMEDLLESACPPAPRHPAGAADAAGTAGSRAAVRCNVAAVALGNAVEFFDFGAYA
TFAVMIGHTFFPAKSPFVSLLLAVSVFGLGFVVRPLGALVIGAYADRAGRKPAMMLTLVMMAVGTGAIAVLPGYETIGVA
APILLVVTRLIQGLAWGGEAGPATTYILEAAPPERRGAYACWQVATQGFAGLAAGLAGYALTLALPEADLYAWGWRVPFA
LGLLVLPIGVYIRRRLSDTIDAHRAYGSTRAILHELNARHRRPIAVGLMILLGSTITQYFLNYMTTFALTELHLPGGVAM
LATFATGAALAVGSLAGGSLSDRVGRRAVLIWPRVLLLLLIFPALQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIA
ESFPQRVRSTGFSIVYAVAVSLFGGTAQSIVTWLIGTTGNPMAPAGYLLVANAICIAAGWLAVETWPSGRNTR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=427, Percent_Identity=30.2107728337237, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788942, Length=378, Percent_Identity=36.7724867724868, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789941, Length=381, Percent_Identity=33.0708661417323, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788292, Length=395, Percent_Identity=30.8860759493671, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082231, Length=233, Percent_Identity=28.3261802575107, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082404, Length=338, Percent_Identity=25.1479289940828, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49867; Mature: 49867

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLYGLYGLRRARGRFRRRFDQGRFMEDLLESACPPAPRHPAGAADAAGTAGSRAAVRC
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEE
NVAAVALGNAVEFFDFGAYATFAVMIGHTFFPAKSPFVSLLLAVSVFGLGFVVRPLGALV
EEHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGAYADRAGRKPAMMLTLVMMAVGTGAIAVLPGYETIGVAAPILLVVTRLIQGLAWGGEA
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GPATTYILEAAPPERRGAYACWQVATQGFAGLAAGLAGYALTLALPEADLYAWGWRVPFA
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCHHHHH
LGLLVLPIGVYIRRRLSDTIDAHRAYGSTRAILHELNARHRRPIAVGLMILLGSTITQYF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LNYMTTFALTELHLPGGVAMLATFATGAALAVGSLAGGSLSDRVGRRAVLIWPRVLLLLL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEHHHHHHHHHH
IFPALQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIAESFPQRVRSTGFSIVYAVAV
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SLFGGTAQSIVTWLIGTTGNPMAPAGYLLVANAICIAAGWLAVETWPSGRNTR
HHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCC
>Mature Secondary Structure
MRSLYGLYGLRRARGRFRRRFDQGRFMEDLLESACPPAPRHPAGAADAAGTAGSRAAVRC
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEE
NVAAVALGNAVEFFDFGAYATFAVMIGHTFFPAKSPFVSLLLAVSVFGLGFVVRPLGALV
EEHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IGAYADRAGRKPAMMLTLVMMAVGTGAIAVLPGYETIGVAAPILLVVTRLIQGLAWGGEA
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
GPATTYILEAAPPERRGAYACWQVATQGFAGLAAGLAGYALTLALPEADLYAWGWRVPFA
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCHHHHH
LGLLVLPIGVYIRRRLSDTIDAHRAYGSTRAILHELNARHRRPIAVGLMILLGSTITQYF
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LNYMTTFALTELHLPGGVAMLATFATGAALAVGSLAGGSLSDRVGRRAVLIWPRVLLLLL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEHHHHHHHHHH
IFPALQLIVSRPTPAVFLATLTVLSGLHGMSGAALIVLIAESFPQRVRSTGFSIVYAVAV
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SLFGGTAQSIVTWLIGTTGNPMAPAGYLLVANAICIAAGWLAVETWPSGRNTR
HHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]