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The map label for this gene is murE [H]

Identifier: 83720483

GI number: 83720483

Start: 1255923

End: 1257467

Strand: Direct

Name: murE [H]

Synonym: BTH_I1113

Alternate gene names: 83720483

Gene position: 1255923-1257467 (Clockwise)

Preceding gene: 83721379

Following gene: 83719896

Centisome position: 32.97

GC content: 71.72

Gene sequence:

>1545_bases
ATGAGCGCCGCGCGCAGTTCCCATCCGGCGCACCAGCAGATCGCGGCCGCGCTTGCGTGGCTGCGCGCGCACGTCGCGTC
CGGCGCGCAATTGCACGCCGACACGCGCAGCCTGCAGGCGGGCGACGTGTTCGTCGCCTATGCGGTCGACGGCGCGGATA
ATCGTCCGTTCATTGCCGACGCGCTCGCGCGCGGCGCGGCCGCCGTGCTGTATCAGCCCGAAGGTTTCCCGGCCGCCGGC
CTCGACCCGGCGACGTCGCTCGCCGTGCCCGCGCTCGACGCGCTCGCGGGCGATATCGCGAGCGGCTGGTACGGCGATCC
GAGCGACGCGATGTTCGCGGTCGGCGTGACGGGCACGAACGGCAAGACGTCGTGCACGCAATGGATCGCGGCCGCGCTCA
CAGCGTTGCGCGAGCCGTGCGCGGTGATCGGCACGCTCGGCAGCGGAATGCCCGGCCAGCTCGTCCACACGGGCTTCACG
ACGCCCGATGCGCCGCAACTGCAGCGCAGCCTCGCGCAACTGCGCGACGCGGGCGCTCAGGCGGTCGCGATGGAAGTCTC
GTCGCATGCGCTGCATCAGGGGCGCGTGAACGGCACCGCGTTCGACGTCGCGGTGTTCACGAACCTCACGCAGGATCACC
TCGATTACCACCGCACGTTCGACGCCTACGAGGCCGCGAAGGCAAAGCTCTTCGCCTGGCGCGGGCTGCATTGCGCGGTT
GTCAATCGCGACGATCCGGCCGGCCGCCGCCTGCTCGCGAATCTCGTCGGCCGCGTGAGGACGATCGCGTACGGCATCGA
TGCGCCGGCGCAAGGCGCCGACGCCGATCGCGAGCTCGTCGCGACGAACGTCCGCGCGACCGCGACGGGCACCGCGTTTC
GCGTGACTTCGCCGTGGGGCGAAGCGGACGTCGAGGTCGGCACGCTCGGCACGTTCAACGTGAGCAATCTGCTCGCGGTG
CTCGGCACGCTGCTCGCGGCCGACGTGCCGTTCGACGCGGCGCTCGCCGAAATCGAGCGGCTCGAATCGGTCAACGGCCG
GATGCAGCGGCTCGGCGGCCGGCTGCAGAACGACGAGCCGCTCGTCGTCGTCGATTACGCGCACACGCCGGACGCGCTCG
AGAAGACGCTCGAAGCGCTGCGCCCGATCGCGCAGGCGCGCGGCGGCGAGCTCGTCTGCATGTTCGGCTGCGGCGGCGAC
CGCGACGCGACGAAGCGCCCGCTGATGGGCGCGATCGCCGAGCGGCTCGCGGACAAGGTCGTCGTGACGAGCGACAACCC
GCGCAGCGAGGAGCCGCAGAAGATCATCGATCAGATCGTCGCCGGCATGCGGGACGCCGCGAACGCGCGCCGAGTCGAGG
ACCGCGCGAGCGCGATCCTGCAGGCCGTGCGCGGCGCCGCGCGCGAGGACGTCGTGCTGCTCGCCGGCAAGGGGCACGAG
GCGACGCAGGAGATCATGGGCAAGAAGCGTGCGTTCTCCGATCAGGATCACGCGCGCCTCGCGCTCGCCGCGCGCACGAC
GCAGACTCGCGGAGGCGGCGAATGA

Upstream 100 bases:

>100_bases
ATACGGCGAACGCCGCCGCTGCGCAGAAGCTGGCCGCGAATGCGCCGGCGAAACACATGATCGTGTCGAGCACGACACAC
AACCGTCCTGGAGTAGTTCG

Downstream 100 bases:

>100_bases
CGATGCTGACCTTGCGCGAAGCCGCAGCGATGATCCCCGGCGCGACCGTGCTCGGCGACGAGCGCGCGTCGTTCGAGCGG
GTGTCGACGGACAGCCGCTC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSAARSSHPAHQQIAAALAWLRAHVASGAQLHADTRSLQAGDVFVAYAVDGADNRPFIADALARGAAAVLYQPEGFPAAG
LDPATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTNGKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFT
TPDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFDAYEAAKAKLFAWRGLHCAV
VNRDDPAGRRLLANLVGRVRTIAYGIDAPAQGADADRELVATNVRATATGTAFRVTSPWGEADVEVGTLGTFNVSNLLAV
LGTLLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEPLVVVDYAHTPDALEKTLEALRPIAQARGGELVCMFGCGGD
RDATKRPLMGAIAERLADKVVVTSDNPRSEEPQKIIDQIVAGMRDAANARRVEDRASAILQAVRGAAREDVVLLAGKGHE
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE

Sequences:

>Translated_514_residues
MSAARSSHPAHQQIAAALAWLRAHVASGAQLHADTRSLQAGDVFVAYAVDGADNRPFIADALARGAAAVLYQPEGFPAAG
LDPATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTNGKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFT
TPDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFDAYEAAKAKLFAWRGLHCAV
VNRDDPAGRRLLANLVGRVRTIAYGIDAPAQGADADRELVATNVRATATGTAFRVTSPWGEADVEVGTLGTFNVSNLLAV
LGTLLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEPLVVVDYAHTPDALEKTLEALRPIAQARGGELVCMFGCGGD
RDATKRPLMGAIAERLADKVVVTSDNPRSEEPQKIIDQIVAGMRDAANARRVEDRASAILQAVRGAAREDVVLLAGKGHE
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE
>Mature_513_residues
SAARSSHPAHQQIAAALAWLRAHVASGAQLHADTRSLQAGDVFVAYAVDGADNRPFIADALARGAAAVLYQPEGFPAAGL
DPATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTNGKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFTT
PDAPQLQRSLAQLRDAGAQAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFDAYEAAKAKLFAWRGLHCAVV
NRDDPAGRRLLANLVGRVRTIAYGIDAPAQGADADRELVATNVRATATGTAFRVTSPWGEADVEVGTLGTFNVSNLLAVL
GTLLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEPLVVVDYAHTPDALEKTLEALRPIAQARGGELVCMFGCGGDR
DATKRPLMGAIAERLADKVVVTSDNPRSEEPQKIIDQIVAGMRDAANARRVEDRASAILQAVRGAAREDVVLLAGKGHEA
TQEIMGKKRAFSDQDHARLALAARTTQTRGGGE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=473, Percent_Identity=45.0317124735729, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54007; Mature: 53876

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARSSHPAHQQIAAALAWLRAHVASGAQLHADTRSLQAGDVFVAYAVDGADNRPFIAD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCCCHHHH
ALARGAAAVLYQPEGFPAAGLDPATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTN
HHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
GKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFTTPDAPQLQRSLAQLRDAGAQ
CCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCC
AVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFDAYEAAKAKLFAWRGLHCAV
EEEHHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCEEEEE
VNRDDPAGRRLLANLVGRVRTIAYGIDAPAQGADADRELVATNVRATATGTAFRVTSPWG
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEECCCEEEEECCCC
EADVEVGTLGTFNVSNLLAVLGTLLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEP
CCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LVVVDYAHTPDALEKTLEALRPIAQARGGELVCMFGCGGDRDATKRPLMGAIAERLADKV
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
VVTSDNPRSEEPQKIIDQIVAGMRDAANARRVEDRASAILQAVRGAAREDVVLLAGKGHE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE
HHHHHHHHHHCCCCCCHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SAARSSHPAHQQIAAALAWLRAHVASGAQLHADTRSLQAGDVFVAYAVDGADNRPFIAD
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCCCHHHH
ALARGAAAVLYQPEGFPAAGLDPATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTN
HHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
GKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFTTPDAPQLQRSLAQLRDAGAQ
CCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCC
AVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFDAYEAAKAKLFAWRGLHCAV
EEEHHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCEEEEE
VNRDDPAGRRLLANLVGRVRTIAYGIDAPAQGADADRELVATNVRATATGTAFRVTSPWG
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEECCCEEEEECCCC
EADVEVGTLGTFNVSNLLAVLGTLLAADVPFDAALAEIERLESVNGRMQRLGGRLQNDEP
CCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LVVVDYAHTPDALEKTLEALRPIAQARGGELVCMFGCGGDRDATKRPLMGAIAERLADKV
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
VVTSDNPRSEEPQKIIDQIVAGMRDAANARRVEDRASAILQAVRGAAREDVVLLAGKGHE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE
HHHHHHHHHHCCCCCCHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]