Click here to switch to the map view.

The map label for this gene is penA [H]

Identifier: 83721379

GI number: 83721379

Start: 1254082

End: 1255926

Strand: Direct

Name: penA [H]

Synonym: BTH_I1112

Alternate gene names: 83721379

Gene position: 1254082-1255926 (Clockwise)

Preceding gene: 83720953

Following gene: 83720483

Centisome position: 32.92

GC content: 64.82

Gene sequence:

>1845_bases
ATGAAGCCCGCTTCAAAGCACACGCAAGGCGTGAAGTTCGCCTCGAGCCCGGTGCTGTCGGTCCATCTGCCGATGTGGCG
CTCGAAGCTCGTCGTGTTCATGCTGTTCATCGCGTTCGTCGCGCTCGCGATGCGCGCGTTCTGGATTCAGGGCCCGGGCA
ACGCGTTCTATCAGAAGCAGGGCGAAAGCCGCTATCAGCGCACCCTCGAATTGCCGGCGACGCGCGGCAAGATCCTCGAT
CGCAACGGCCTCGTGCTCGCGACGAGCCTGCCCGTGCGCGCGATCTGGGCGATTCCCGCCGACGTGCCCGACGATCTCGG
CGCGGACAAGCTCGACGCGCTCGGCAAATTGCTCGGAATGACGACGAAGGAGCTGAAGGCGAAGCTGTCGGAAGACAAGT
CGTTCGTCTACGTGAAGCGCCAGGTGCCGCTCGACGTGGCCGCCGAGGTCGCGAAGCTCGACATCCCGGGCATCTATCAG
CGCAACGAATACAAGCGCTTCTATCCGGAAGGCGAGATCACCGCGCACCTGATCGGCTTCACGAACGTCGAGGACGAAGG
GCAGGAGGGCGTCGAGCTCGCCGACCAGAAGCTGCTCGTCGGCACGCCGGGCAGCCGCCACGTGATCAAGGACCGGGTCG
GCCATATCGTCGAGGACGTCGATGCGCAGGTGCCGCCGCACAACGGCACCGACGTCGACCTGTCGATCGACAGCAAGATC
CAGTACATCACGTATACGAACCTGAAGGATGCGGTCGAGAAGTTCCACGCGAAAGCAGGCGCGGCGATGGTGGTCGACGT
GCAGACGGGCGAGGTGTTGTCGCTCGTCAATTATCCGACGTACAACCCGAACGACCGCTCGCACCTGACGGGCGAGCAAC
TGCGCAACCGCGTGCTGACCGATGTGTTCGAGCCGGGCTCGATCATGAAGCCGTTTACGGTGTCGCTCGCGCTCGACTTG
CATCGCGTGACGCCGAACACGCTCGTCGAGACGGGTAACGGCCATTTCGTGCTCGACGGCGCGCCGATCACCGACGATTC
GGGCTTCGGCACGCTGACCGTCGGCGGCGTGATCCAGAAATCGAGCAATATCGGCGCGACGAAGATCGCGATGACGATGA
AGCCCGAGGAAATGTGGAATATGTATACGGGCATTGGGCTCGGGCAGGCGCCGAAGGTTGGCTTTCCCGGCGCGGCGGCG
GGCCGGCTGCGTCCGTGGAAGAGCTGGCGGCGCATCGAGCAGGCGACGATGTCGTACGGCTACGGCCTGTCGGTGTCGCT
GTTCCAGCTTGCGCGCGCGTACACCGCGATCGCGCACGACGGCGAGCTGATGCCCGTGACGATCTTCAAGACGAACGACG
CGCAGCCCGCGACCGGGCCGCGCATCTTCTCGCCGACGACGGCTCGTGAAGTGCGCACGATGCTCGAATCCGTCGTGTCG
CCGCAGGGTACGTCGCCGAATGCGGCGGTGCCCGGCTACCGCGTCGGCGGCAAGAGCGGCACTGCTTACAAGCAGGTCGG
CCGCGGCTATGATCACAAGAAGTATCGTGCGTCGTTCGTCGGGATGGCGCCGATGCCGAATCCGCGCATCGTCGTCGCCG
TGTCGGTCGACGAGCCGACGGTGGGCGGCCACTTCGGCGGCCAGGTGTCAGGCCCCGTGTTCTCCGCGATCGTCGGCGAT
ACGCTGCGCGCGCTGAATGTACCGCCCAACATGCCGGTCAAGCAGCTCGTCGTGTCTGACGATACGGCGAACGCCGCCGC
TGCGCAGAAGCTGGCCGCGAATGCGCCGGCGAAACACATGATCGTGTCGAGCACGACACACAACCGTCCTGGAGTAGTTC
GATGA

Upstream 100 bases:

>100_bases
TCACGACGGGGCGCACCCAATATCTGACGCTCTCGCCCGGCGCGGCGAAGGCGGTCGACGCGCCGCTGCCGGCATCCGCC
GCGCCGCAGGGAGGCGCGCG

Downstream 100 bases:

>100_bases
GCGCCGCGCGCAGTTCCCATCCGGCGCACCAGCAGATCGCGGCCGCGCTTGCGTGGCTGCGCGCGCACGTCGCGTCCGGC
GCGCAATTGCACGCCGACAC

Product: penicillin-binding protein

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MKPASKHTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILD
RNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGMTTKELKAKLSEDKSFVYVKRQVPLDVAAEVAKLDIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGTDVDLSIDSKI
QYITYTNLKDAVEKFHAKAGAAMVVDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQKSSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPATGPRIFSPTTAREVRTMLESVVS
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPTVGGHFGGQVSGPVFSAIVGD
TLRALNVPPNMPVKQLVVSDDTANAAAAQKLAANAPAKHMIVSSTTHNRPGVVR

Sequences:

>Translated_614_residues
MKPASKHTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILD
RNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGMTTKELKAKLSEDKSFVYVKRQVPLDVAAEVAKLDIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGTDVDLSIDSKI
QYITYTNLKDAVEKFHAKAGAAMVVDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQKSSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPATGPRIFSPTTAREVRTMLESVVS
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPTVGGHFGGQVSGPVFSAIVGD
TLRALNVPPNMPVKQLVVSDDTANAAAAQKLAANAPAKHMIVSSTTHNRPGVVR
>Mature_614_residues
MKPASKHTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILD
RNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGMTTKELKAKLSEDKSFVYVKRQVPLDVAAEVAKLDIPGIYQ
RNEYKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGTDVDLSIDSKI
QYITYTNLKDAVEKFHAKAGAAMVVDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQKSSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAA
GRLRPWKSWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPATGPRIFSPTTAREVRTMLESVVS
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPTVGGHFGGQVSGPVFSAIVGD
TLRALNVPPNMPVKQLVVSDDTANAAAAQKLAANAPAKHMIVSSTTHNRPGVVR

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=553, Percent_Identity=41.2296564195298, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1786854, Length=585, Percent_Identity=24.6153846153846, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 66418; Mature: 66418

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASKHTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQ
CCCCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHCC
GESRYQRTLELPATRGKILDRNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGM
CHHHHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
TTKELKAKLSEDKSFVYVKRQVPLDVAAEVAKLDIPGIYQRNEYKRFYPEGEITAHLIGF
CHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCEEEEEEEE
TNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGTDVDLSIDSKI
CCCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEE
QYITYTNLKDAVEKFHAKAGAAMVVDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLT
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
DVFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQK
HHCCCCCCCCCEEEEEEEEEECCCCCCEEECCCCEEEECCCCCCCCCCCEEEEECCEEEC
SSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPATGPRIFSPTTAREVRTMLESVVS
CCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPT
CCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCCCEEEEEECCCCC
VGGHFGGQVSGPVFSAIVGDTLRALNVPPNMPVKQLVVSDDTANAAAAQKLAANAPAKHM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHHHHCCCCCCEE
IVSSTTHNRPGVVR
EEECCCCCCCCCCC
>Mature Secondary Structure
MKPASKHTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQ
CCCCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHCC
GESRYQRTLELPATRGKILDRNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGM
CHHHHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
TTKELKAKLSEDKSFVYVKRQVPLDVAAEVAKLDIPGIYQRNEYKRFYPEGEITAHLIGF
CHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCEEEEEEEE
TNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGTDVDLSIDSKI
CCCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEE
QYITYTNLKDAVEKFHAKAGAAMVVDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLT
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
DVFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQK
HHCCCCCCCCCEEEEEEEEEECCCCCCEEECCCCEEEECCCCCCCCCCCEEEEECCEEEC
SSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAAGRLRPWKSWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPATGPRIFSPTTAREVRTMLESVVS
CCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPT
CCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCCCEEEEEECCCCC
VGGHFGGQVSGPVFSAIVGDTLRALNVPPNMPVKQLVVSDDTANAAAAQKLAANAPAKHM
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEEECCCCHHHHHHHHHCCCCCCEE
IVSSTTHNRPGVVR
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]