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The map label for this gene is yfnG [H]

Identifier: 83590429

GI number: 83590429

Start: 1623596

End: 1624537

Strand: Reverse

Name: yfnG [H]

Synonym: Moth_1589

Alternate gene names: 83590429

Gene position: 1624537-1623596 (Counterclockwise)

Preceding gene: 83590430

Following gene: 83590428

Centisome position: 61.8

GC content: 57.75

Gene sequence:

>942_bases
ATGGAGCTTCTGATAACTGGCGGGTCTGGTGACGTCGGACGGTACCTGGTCCGGGATTTAGCCGGCCGCGGCCATCGGGT
ACGGGTCCTGGACCGGGCTCTACCTAACGGTGATGGCCTCCCTGTCAGCCAAGAGACTCTTTTTAAAGGCCAGCTGGAGG
ATAAGGAACTAGTAGTCAGGGCTGTTAAAGGGGTTGAAGCGGTAATTCACCTGGCCTGGAGCTTCAGCGACGACCCCCTG
GAGGTTTTCGGCGGCGACCTGATTGGGCATATAAATCTCTTAACAGCGGCTACCAGGGCTGGGGTGAAGCACTTTATTTA
TGCCAGTACCGCTACAGTTTACGGCCGGGCTGCCGGGCATCCAGTTGTAGAAGAACATCCCTGCCTGGTGGGAGAAGCGC
GTAAACCCCTTTATGCCCTGGGCAAGTTTGCCGCCGAGGAGCTGTGCCGTCAGTACTGCCGTGAACAGGGATTGCCGGTG
ACCATTTTCCGTTTCTGGTGGGCCTTTGGCGATGAGATTGGCGGTCGCCATTTGCGCAACCTCATACGGGCGGCCCTCAA
TGAGGAACCCATCAAGGTACCAGTCGCTGCCGGGGGCACCTTTGTCAGTATGGCTGACCTGGCTGCCGCCTGCCGGCTGG
TCCTGGCGGGGGAAGGGGCTTGCGGCCAGGTCTATAACCTGGGCAGCCTGTATTTGACCTGGGAAGAGATCGCCAGCAAG
ATAATTGAACTTACCGGTTCCGCGGGGGAGCTACAACTGGTACCCCAGAATGAATGGACAGGACCGGCTTTCTTAAACGA
AGTCTGGGATCTCAGCTGGGAAAAGGCGGCCCGGGAATTGGGCTATCGACCTACCCTCACCGTCGATGAGGGTCGGTTGG
CCTTTACCAGGGCATTGCTTCGCTGTGTGGATAAAGTCCGAACGGAAATGGGGAAAAACTAG

Upstream 100 bases:

>100_bases
AAGCAGTGGTTTTTCCAGCCAGGCTTAATAATAAGTTGAGAGTTCCGCTATAAGCAGGGGTACCTGGTACCCCTGCTTAT
GCTAAAAGGAGGCATTAACC

Downstream 100 bases:

>100_bases
AAAAGCTTAACAAAAAGGTAGGGAGAGTGGTACAATGGAAAAGCGGGATTAAAAAAGGAGCATGTTGGGGTTACAGAAAT
GGCACGTGTGGAAACTGTAC

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MELLITGGSGDVGRYLVRDLAGRGHRVRVLDRALPNGDGLPVSQETLFKGQLEDKELVVRAVKGVEAVIHLAWSFSDDPL
EVFGGDLIGHINLLTAATRAGVKHFIYASTATVYGRAAGHPVVEEHPCLVGEARKPLYALGKFAAEELCRQYCREQGLPV
TIFRFWWAFGDEIGGRHLRNLIRAALNEEPIKVPVAAGGTFVSMADLAAACRLVLAGEGACGQVYNLGSLYLTWEEIASK
IIELTGSAGELQLVPQNEWTGPAFLNEVWDLSWEKAARELGYRPTLTVDEGRLAFTRALLRCVDKVRTEMGKN

Sequences:

>Translated_313_residues
MELLITGGSGDVGRYLVRDLAGRGHRVRVLDRALPNGDGLPVSQETLFKGQLEDKELVVRAVKGVEAVIHLAWSFSDDPL
EVFGGDLIGHINLLTAATRAGVKHFIYASTATVYGRAAGHPVVEEHPCLVGEARKPLYALGKFAAEELCRQYCREQGLPV
TIFRFWWAFGDEIGGRHLRNLIRAALNEEPIKVPVAAGGTFVSMADLAAACRLVLAGEGACGQVYNLGSLYLTWEEIASK
IIELTGSAGELQLVPQNEWTGPAFLNEVWDLSWEKAARELGYRPTLTVDEGRLAFTRALLRCVDKVRTEMGKN
>Mature_313_residues
MELLITGGSGDVGRYLVRDLAGRGHRVRVLDRALPNGDGLPVSQETLFKGQLEDKELVVRAVKGVEAVIHLAWSFSDDPL
EVFGGDLIGHINLLTAATRAGVKHFIYASTATVYGRAAGHPVVEEHPCLVGEARKPLYALGKFAAEELCRQYCREQGLPV
TIFRFWWAFGDEIGGRHLRNLIRAALNEEPIKVPVAAGGTFVSMADLAAACRLVLAGEGACGQVYNLGSLYLTWEEIASK
IIELTGSAGELQLVPQNEWTGPAFLNEVWDLSWEKAARELGYRPTLTVDEGRLAFTRALLRCVDKVRTEMGKN

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=310, Percent_Identity=29.6774193548387, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1786974, Length=330, Percent_Identity=26.0606060606061, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 34241; Mature: 34241

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLITGGSGDVGRYLVRDLAGRGHRVRVLDRALPNGDGLPVSQETLFKGQLEDKELVVR
CEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCHHHHHCCCCCHHHHHHH
AVKGVEAVIHLAWSFSDDPLEVFGGDLIGHINLLTAATRAGVKHFIYASTATVYGRAAGH
HHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCCC
PVVEEHPCLVGEARKPLYALGKFAAEELCRQYCREQGLPVTIFRFWWAFGDEIGGRHLRN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHH
LIRAALNEEPIKVPVAAGGTFVSMADLAAACRLVLAGEGACGQVYNLGSLYLTWEEIASK
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHEECCCCCCCCEECCHHEEEHHHHHHH
IIELTGSAGELQLVPQNEWTGPAFLNEVWDLSWEKAARELGYRPTLTVDEGRLAFTRALL
HHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEECCCHHHHHHHHH
RCVDKVRTEMGKN
HHHHHHHHHHCCC
>Mature Secondary Structure
MELLITGGSGDVGRYLVRDLAGRGHRVRVLDRALPNGDGLPVSQETLFKGQLEDKELVVR
CEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHCCCCCCCCCCCHHHHHCCCCCHHHHHHH
AVKGVEAVIHLAWSFSDDPLEVFGGDLIGHINLLTAATRAGVKHFIYASTATVYGRAAGH
HHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCCC
PVVEEHPCLVGEARKPLYALGKFAAEELCRQYCREQGLPVTIFRFWWAFGDEIGGRHLRN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHH
LIRAALNEEPIKVPVAAGGTFVSMADLAAACRLVLAGEGACGQVYNLGSLYLTWEEIASK
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHEECCCCCCCCEECCHHEEEHHHHHHH
IIELTGSAGELQLVPQNEWTGPAFLNEVWDLSWEKAARELGYRPTLTVDEGRLAFTRALL
HHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHCCCCCCEEECCCHHHHHHHHH
RCVDKVRTEMGKN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]