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The map label for this gene is cisA [H]

Identifier: 83590404

GI number: 83590404

Start: 1603696

End: 1605336

Strand: Reverse

Name: cisA [H]

Synonym: Moth_1561

Alternate gene names: 83590404

Gene position: 1605336-1603696 (Counterclockwise)

Preceding gene: 83590405

Following gene: 83590403

Centisome position: 61.07

GC content: 51.55

Gene sequence:

>1641_bases
ATGAAAGCCGTGATATATGCTAGGGTAAGCACCCAGGACCAGGCTCTCGGTTTTTCCCTGGCCACCCAGAAAGAACTCTG
CGAGAAGAAGGCCAGGGCTTTAGGGGCTTTAGAAGTCGAAGTTGTTGAAGACGCTTACACCGGGACGGAATTGAACCGCC
CTAGCCTGGATTATGTGAGGCAGCTCGTAGCTGCCGGGAAAGTGGACCTTGTAGTTGTATATGACCCCGACAGGCTTTCC
CGCAACCTTACAGATTTGCTCATCCTTTGCCGGGAATTTGATAAGGCAGGAGTAAGGCTGGAGTTTGTTAATTTTGACTG
GCAAAAGACGCCCCAGGGGATGCTCTTCTTGCAGATGCGGGGGGCCTTTGCCGAATTCGAGCATGCGCTCATCAAGGAGC
GTACCCAGAGGGGCAAGGCCAAAAAAGCGGCCAGCGGCAAGATCCGCTGCTACGCCAAGCCTTTTGGTTACGACTGGGAC
GCCGAGAGGGATACCCTGGTAATTAATCCCCAGGAAGCCGCAATCGTCAAGCAGATGTTTGAGTGGCTAACGGATCCCCT
GGAACCCTTAACTCCCTGGCAGATTACCCAGAAGCTGGGCCAGGTGTATCCCCAGGGTCCCAGGGGCAAGGGGTGGGTCC
ACTCTTCGGTGGTACGGATGCTGAAAAATCCCGTATATACAGGCCGGTTGAGGCGCAAGGATGAGCAGCCTGATTGGAAA
CCGGTCCTTGTGCCGGCCATAATTGACCAGGAAACCTTTATGAGAGCCCAGGAGATGCTTGCCAGGTCCAAGCGTTTCAA
CCCTAAGACTACCAGGAGAAGATTCCTGTTGCAGCGGTTGCTGGTATGCGGGGAGTGCGGGCGGAGGCTTACAGTCGTCA
CCCATAACAATCCCCAGAGGGCGAAGTATAGCTATTATACCTGTCCCGGGCGTTACCCTACCAAGTTTGACGACCAGGGC
CGGGTAGGCAGATGCGGGCTCCCACCCTGGCGGACGGAAGAAATAGATAAGACAGTATGGGATACTATAGCTTCCATAAT
TAAGAACCCGGAGCTTTTCTACCAGTATATTACCAGCGAAAAGCTTGAAACAGCCAGCATTCCCCGGAGGCGCCTGGAGG
AAGCGCGGAAGAGGCTAGAGCAGGTGCAACGGGTGATCGAGCGGATTGACCGGGCCTATTTTATCCTGGAGGCGTTGCCC
GAAGAAGACTACAAGCGTTACCGGGCGGAACAAGAAGGAGAGCTAGTAAGAATAGAAGAAGATATCAAGAGGCTTGAAGC
CGTAATTAACGCCCAGGAACAAGTGCAAAAAGGCGTAGAATTCCTGCGCCAGTACGCTGAAAACCTGGCAAGGTCGGTGG
ATGAACTAAACTTTTTTCAAAAGCAGAATATCACCCGCGAGCTAGTGCAGAGGGTAAAAATATATGCTGACGGCAGCCTG
GAGATAGAAGGGTACTTTAACATCCCCTTTACCGGGCCGGGTAAAAATGTTTCGGATCATTGCGAAGAACCTACAACATT
AACCACACAGAAAAACTTAAAAGAACCACTTTCAAGTAGTGTTTTAACTTGCACACATCGAAAAAAAGGCCATCCAAAAG
TTACTGAAGGAATTATCCATCTGACGATTTTAGCTGTATAA

Upstream 100 bases:

>100_bases
GTAGTTGATATTATACGCCAGGCCTGCCCTGACAGGCAGGAAAACCCGCAAGAGATGTTGAAGGAAATAAACATGAATCC
TGCTACGGGGAAGAAGAAAG

Downstream 100 bases:

>100_bases
TAGCCTGGGGATAAAAGCTCCTTGTTTTACGAAGGTTAGACCCACTAGAAGGGAAGGGTGCACAGGGTTTGCAGCAGGAA
AAAAAGGCCGCCGGTGGCCC

Product: resolvase-like protein

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKAVIYARVSTQDQALGFSLATQKELCEKKARALGALEVEVVEDAYTGTELNRPSLDYVRQLVAAGKVDLVVVYDPDRLS
RNLTDLLILCREFDKAGVRLEFVNFDWQKTPQGMLFLQMRGAFAEFEHALIKERTQRGKAKKAASGKIRCYAKPFGYDWD
AERDTLVINPQEAAIVKQMFEWLTDPLEPLTPWQITQKLGQVYPQGPRGKGWVHSSVVRMLKNPVYTGRLRRKDEQPDWK
PVLVPAIIDQETFMRAQEMLARSKRFNPKTTRRRFLLQRLLVCGECGRRLTVVTHNNPQRAKYSYYTCPGRYPTKFDDQG
RVGRCGLPPWRTEEIDKTVWDTIASIIKNPELFYQYITSEKLETASIPRRRLEEARKRLEQVQRVIERIDRAYFILEALP
EEDYKRYRAEQEGELVRIEEDIKRLEAVINAQEQVQKGVEFLRQYAENLARSVDELNFFQKQNITRELVQRVKIYADGSL
EIEGYFNIPFTGPGKNVSDHCEEPTTLTTQKNLKEPLSSSVLTCTHRKKGHPKVTEGIIHLTILAV

Sequences:

>Translated_546_residues
MKAVIYARVSTQDQALGFSLATQKELCEKKARALGALEVEVVEDAYTGTELNRPSLDYVRQLVAAGKVDLVVVYDPDRLS
RNLTDLLILCREFDKAGVRLEFVNFDWQKTPQGMLFLQMRGAFAEFEHALIKERTQRGKAKKAASGKIRCYAKPFGYDWD
AERDTLVINPQEAAIVKQMFEWLTDPLEPLTPWQITQKLGQVYPQGPRGKGWVHSSVVRMLKNPVYTGRLRRKDEQPDWK
PVLVPAIIDQETFMRAQEMLARSKRFNPKTTRRRFLLQRLLVCGECGRRLTVVTHNNPQRAKYSYYTCPGRYPTKFDDQG
RVGRCGLPPWRTEEIDKTVWDTIASIIKNPELFYQYITSEKLETASIPRRRLEEARKRLEQVQRVIERIDRAYFILEALP
EEDYKRYRAEQEGELVRIEEDIKRLEAVINAQEQVQKGVEFLRQYAENLARSVDELNFFQKQNITRELVQRVKIYADGSL
EIEGYFNIPFTGPGKNVSDHCEEPTTLTTQKNLKEPLSSSVLTCTHRKKGHPKVTEGIIHLTILAV
>Mature_546_residues
MKAVIYARVSTQDQALGFSLATQKELCEKKARALGALEVEVVEDAYTGTELNRPSLDYVRQLVAAGKVDLVVVYDPDRLS
RNLTDLLILCREFDKAGVRLEFVNFDWQKTPQGMLFLQMRGAFAEFEHALIKERTQRGKAKKAASGKIRCYAKPFGYDWD
AERDTLVINPQEAAIVKQMFEWLTDPLEPLTPWQITQKLGQVYPQGPRGKGWVHSSVVRMLKNPVYTGRLRRKDEQPDWK
PVLVPAIIDQETFMRAQEMLARSKRFNPKTTRRRFLLQRLLVCGECGRRLTVVTHNNPQRAKYSYYTCPGRYPTKFDDQG
RVGRCGLPPWRTEEIDKTVWDTIASIIKNPELFYQYITSEKLETASIPRRRLEEARKRLEQVQRVIERIDRAYFILEALP
EEDYKRYRAEQEGELVRIEEDIKRLEAVINAQEQVQKGVEFLRQYAENLARSVDELNFFQKQNITRELVQRVKIYADGSL
EIEGYFNIPFTGPGKNVSDHCEEPTTLTTQKNLKEPLSSSVLTCTHRKKGHPKVTEGIIHLTILAV

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=145, Percent_Identity=33.1034482758621, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 63188; Mature: 63188

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVIYARVSTQDQALGFSLATQKELCEKKARALGALEVEVVEDAYTGTELNRPSLDYVR
CCEEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCHHHHH
QLVAAGKVDLVVVYDPDRLSRNLTDLLILCREFDKAGVRLEFVNFDWQKTPQGMLFLQMR
HHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEH
GAFAEFEHALIKERTQRGKAKKAASGKIRCYAKPFGYDWDAERDTLVINPQEAAIVKQMF
HHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHH
EWLTDPLEPLTPWQITQKLGQVYPQGPRGKGWVHSSVVRMLKNPVYTGRLRRKDEQPDWK
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCC
PVLVPAIIDQETFMRAQEMLARSKRFNPKTTRRRFLLQRLLVCGECGRRLTVVTHNNPQR
CEEEHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AKYSYYTCPGRYPTKFDDQGRVGRCGLPPWRTEEIDKTVWDTIASIIKNPELFYQYITSE
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHC
KLETASIPRRRLEEARKRLEQVQRVIERIDRAYFILEALPEEDYKRYRAEQEGELVRIEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEHHH
DIKRLEAVINAQEQVQKGVEFLRQYAENLARSVDELNFFQKQNITRELVQRVKIYADGSL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCE
EIEGYFNIPFTGPGKNVSDHCEEPTTLTTQKNLKEPLSSSVLTCTHRKKGHPKVTEGIIH
EEEEEEECCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHEE
LTILAV
EEEEEC
>Mature Secondary Structure
MKAVIYARVSTQDQALGFSLATQKELCEKKARALGALEVEVVEDAYTGTELNRPSLDYVR
CCEEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCHHHHH
QLVAAGKVDLVVVYDPDRLSRNLTDLLILCREFDKAGVRLEFVNFDWQKTPQGMLFLQMR
HHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEH
GAFAEFEHALIKERTQRGKAKKAASGKIRCYAKPFGYDWDAERDTLVINPQEAAIVKQMF
HHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHH
EWLTDPLEPLTPWQITQKLGQVYPQGPRGKGWVHSSVVRMLKNPVYTGRLRRKDEQPDWK
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCC
PVLVPAIIDQETFMRAQEMLARSKRFNPKTTRRRFLLQRLLVCGECGRRLTVVTHNNPQR
CEEEHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
AKYSYYTCPGRYPTKFDDQGRVGRCGLPPWRTEEIDKTVWDTIASIIKNPELFYQYITSE
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHC
KLETASIPRRRLEEARKRLEQVQRVIERIDRAYFILEALPEEDYKRYRAEQEGELVRIEE
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEHHH
DIKRLEAVINAQEQVQKGVEFLRQYAENLARSVDELNFFQKQNITRELVQRVKIYADGSL
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCE
EIEGYFNIPFTGPGKNVSDHCEEPTTLTTQKNLKEPLSSSVLTCTHRKKGHPKVTEGIIH
EEEEEEECCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHEE
LTILAV
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]