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The map label for this gene is pycB [H]

Identifier: 83590368

GI number: 83590368

Start: 1570431

End: 1572362

Strand: Reverse

Name: pycB [H]

Synonym: Moth_1525

Alternate gene names: 83590368

Gene position: 1572362-1570431 (Counterclockwise)

Preceding gene: 83590369

Following gene: 83590367

Centisome position: 59.81

GC content: 60.46

Gene sequence:

>1932_bases
ATGGCGGTTGCAGAGCCAGCCATGGGGAATGTCGCCCTGGCTTTAGCCAGGGTTTTTCTTTACGGGGGAGGAGCAGTTTT
GAGCCAGTTGAAGATTACAGACACCACCTTGCGCGATGGCCACCAGAGCCTTTGGGCCACCAGGATGACTACGGCCGATA
TGCTGCCCATTATCGAAAAGATCGATAGTGTCGGGTACCACTCCCTGGAGGTCTGGGGTGGGGCCACCTTCGATGTCTGC
ATGCGCTTCCTGGATGAGGATCCCTGGGAGCGCCTCCGTACCTTGAAAAAATATGCCCGGCGGACGCCCCTGCAGATGCT
TCTCCGGGCCCAGTCCCTGGTGGGCTACCAGCTGTACCCGGACGATGTAGTACGGGCCTTTATCGCCAGGGCCGCGGCCA
ACGGCATTGATATTATCCGCATTTTCGACGCCCTGAACGACCTGCGCAACATGGAAGTCCCGGTGGAAGCCGCCAAAAAG
GAAGGTGTCCACGTCCAGGGAACGGTGGTCTATACCATCAGCCCGGTGCATACCACCGAGCACTACCTGAAAACAGCCCT
GGAACTGGAGAGTATGGGGGTCGATTCCATTTGCATTAAAGATATGGCCGGCCTCCTGGCTCCCTTTGAAGCCTATAAAC
TGGTCAAGCTCTTTAAAGAAAAACTCCACGTCCCCGTTCAGCTCCATAGCCATTACATCGGCGGTCTGGCGGTTGGGGCC
TACCTGGAAGCGGCCCGGGCCGGGGTGGATGTCGTCGACACAGCCTCTGTTCCCCTGGCCTTCGGCGCCTCCCAGCCGCC
GGTGGAGACGGTGGTCCGGGCCCTGGAGGGAACGCCCTATGACACCGGCCTGGATCTCAACCTCCTCTTTGAAATCGCCC
GCTATTTCGACGACCTGCGCCGGGAACTGGGTTACGAGCGCGGCGTCACCCGGATTACCGATATGTGGGTTTTCCAGCAC
CAGGTCCCCGGGGGCATGATCTCCAACCTGGTCAGCCAGTTAAAGGAACAAAAGGCCGCCGACCGGATCAACGAGGTCCT
GGCCGAGATACCCCGGGTACGGGCCGACCTGGGTTACCCGCCCCTGGTCACGCCCACCAGCCAGATTGTCGGTACCCAGG
CGGTTTTAAACGTCCTCCTGGGCGAACGTTACAAGATGGTACCCGGCGAGGTGAAGAATTACGTCCGGGGCCTGTACGGC
CGGCCGCCGGCACCCATTTCCGAGGAGATCCGGCGCCTGATAATCGGCGATGAAGAGCCCATCCAGGGGCGACCGGCCGA
CATCCTGGAGCCCCGCCTGGAGGAAGCGCGGCGGGAGATCGGCGATCTGGCCCGTAATGAGGACGACGTGGTTGCCTACG
CCATGTTTCCCCAGATTGCCCGGAAATTCTTTGAGAAGCGCCAGCAGGGCCAGCTCCGGCCGGGCCGGCAGTCTTTACCA
TCCAGGGGCCAGGATAAGGCAGAGGCAGGAGGTACAGCAAAGATGGATCTCAAGGATATCACCCAGCTCATTAAAGCCCT
GGAAGAGACGGGGATTACCGAACTGAACCTGGAGAGCGAAGGGGTAAAGGTCATGATCCGCCGCGGTAGCGGCCAGGGGG
CGGCGGAGATCCCCGCACCGGAGATAAAAACTGCTGCAGAGGTACTGGCCACAGACGGCGGCGCTCAGGAAACACCTGTC
CCGGCCGGAGATATCATTGAAGTCCGGGCGCCCATGGTGGGTACCTTTTACCGGGCTCCCTCTCCCGACGCGCCCCCTTT
TGTCGAGGTGGGGACCAGGGTTAAGGCAGGACAAACCCTGTGCATAATAGAGGCGATGAAGCTAATGAACGAGCTCACGG
CTGAAACCGGCGGCCAGGTGGTGGCCATCCTGGCCGAAAACGGCCAGCCGGTGGAATACGGCCAGGTCTTGTTCCAGATC
AAGAAGGATTAA

Upstream 100 bases:

>100_bases
TTGAAAAACCGGCGCACGGTATTGCAGCTTAAAGCACAAATATATTGTATACTTGTTCTCGGGGTATTGAATGATGGGAT
TGCTATTTATATAATAGGGC

Downstream 100 bases:

>100_bases
AGTCTGCACAACTCTGGAGGCTAGCGATGTTTAGAAGGGTTTTAGTTGCCAACCGCGGCGAGATTGCCGTACGGATTATC
CGGGCCTGCCGGGAACTGGA

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MAVAEPAMGNVALALARVFLYGGGAVLSQLKITDTTLRDGHQSLWATRMTTADMLPIIEKIDSVGYHSLEVWGGATFDVC
MRFLDEDPWERLRTLKKYARRTPLQMLLRAQSLVGYQLYPDDVVRAFIARAAANGIDIIRIFDALNDLRNMEVPVEAAKK
EGVHVQGTVVYTISPVHTTEHYLKTALELESMGVDSICIKDMAGLLAPFEAYKLVKLFKEKLHVPVQLHSHYIGGLAVGA
YLEAARAGVDVVDTASVPLAFGASQPPVETVVRALEGTPYDTGLDLNLLFEIARYFDDLRRELGYERGVTRITDMWVFQH
QVPGGMISNLVSQLKEQKAADRINEVLAEIPRVRADLGYPPLVTPTSQIVGTQAVLNVLLGERYKMVPGEVKNYVRGLYG
RPPAPISEEIRRLIIGDEEPIQGRPADILEPRLEEARREIGDLARNEDDVVAYAMFPQIARKFFEKRQQGQLRPGRQSLP
SRGQDKAEAGGTAKMDLKDITQLIKALEETGITELNLESEGVKVMIRRGSGQGAAEIPAPEIKTAAEVLATDGGAQETPV
PAGDIIEVRAPMVGTFYRAPSPDAPPFVEVGTRVKAGQTLCIIEAMKLMNELTAETGGQVVAILAENGQPVEYGQVLFQI
KKD

Sequences:

>Translated_643_residues
MAVAEPAMGNVALALARVFLYGGGAVLSQLKITDTTLRDGHQSLWATRMTTADMLPIIEKIDSVGYHSLEVWGGATFDVC
MRFLDEDPWERLRTLKKYARRTPLQMLLRAQSLVGYQLYPDDVVRAFIARAAANGIDIIRIFDALNDLRNMEVPVEAAKK
EGVHVQGTVVYTISPVHTTEHYLKTALELESMGVDSICIKDMAGLLAPFEAYKLVKLFKEKLHVPVQLHSHYIGGLAVGA
YLEAARAGVDVVDTASVPLAFGASQPPVETVVRALEGTPYDTGLDLNLLFEIARYFDDLRRELGYERGVTRITDMWVFQH
QVPGGMISNLVSQLKEQKAADRINEVLAEIPRVRADLGYPPLVTPTSQIVGTQAVLNVLLGERYKMVPGEVKNYVRGLYG
RPPAPISEEIRRLIIGDEEPIQGRPADILEPRLEEARREIGDLARNEDDVVAYAMFPQIARKFFEKRQQGQLRPGRQSLP
SRGQDKAEAGGTAKMDLKDITQLIKALEETGITELNLESEGVKVMIRRGSGQGAAEIPAPEIKTAAEVLATDGGAQETPV
PAGDIIEVRAPMVGTFYRAPSPDAPPFVEVGTRVKAGQTLCIIEAMKLMNELTAETGGQVVAILAENGQPVEYGQVLFQI
KKD
>Mature_642_residues
AVAEPAMGNVALALARVFLYGGGAVLSQLKITDTTLRDGHQSLWATRMTTADMLPIIEKIDSVGYHSLEVWGGATFDVCM
RFLDEDPWERLRTLKKYARRTPLQMLLRAQSLVGYQLYPDDVVRAFIARAAANGIDIIRIFDALNDLRNMEVPVEAAKKE
GVHVQGTVVYTISPVHTTEHYLKTALELESMGVDSICIKDMAGLLAPFEAYKLVKLFKEKLHVPVQLHSHYIGGLAVGAY
LEAARAGVDVVDTASVPLAFGASQPPVETVVRALEGTPYDTGLDLNLLFEIARYFDDLRRELGYERGVTRITDMWVFQHQ
VPGGMISNLVSQLKEQKAADRINEVLAEIPRVRADLGYPPLVTPTSQIVGTQAVLNVLLGERYKMVPGEVKNYVRGLYGR
PPAPISEEIRRLIIGDEEPIQGRPADILEPRLEEARREIGDLARNEDDVVAYAMFPQIARKFFEKRQQGQLRPGRQSLPS
RGQDKAEAGGTAKMDLKDITQLIKALEETGITELNLESEGVKVMIRRGSGQGAAEIPAPEIKTAAEVLATDGGAQETPVP
AGDIIEVRAPMVGTFYRAPSPDAPPFVEVGTRVKAGQTLCIIEAMKLMNELTAETGGQVVAILAENGQPVEYGQVLFQIK
KD

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=644, Percent_Identity=30.5900621118012, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI106049295, Length=644, Percent_Identity=30.5900621118012, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI106049292, Length=644, Percent_Identity=30.5900621118012, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1789653, Length=160, Percent_Identity=44.375, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17562816, Length=646, Percent_Identity=32.6625386996904, Blast_Score=272, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6319695, Length=644, Percent_Identity=31.832298136646, Blast_Score=269, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6321376, Length=637, Percent_Identity=32.0251177394035, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24652212, Length=648, Percent_Identity=32.4074074074074, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24652210, Length=648, Percent_Identity=32.4074074074074, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24652214, Length=648, Percent_Identity=32.4074074074074, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI19921944, Length=648, Percent_Identity=32.4074074074074, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24652216, Length=648, Percent_Identity=32.4074074074074, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI281363050, Length=648, Percent_Identity=32.7160493827161, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24652224, Length=648, Percent_Identity=32.7160493827161, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24652222, Length=648, Percent_Identity=32.7160493827161, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24652220, Length=648, Percent_Identity=32.7160493827161, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24652218, Length=648, Percent_Identity=32.7160493827161, Blast_Score=259, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 70600; Mature: 70469

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAEPAMGNVALALARVFLYGGGAVLSQLKITDTTLRDGHQSLWATRMTTADMLPIIEK
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDSVGYHSLEVWGGATFDVCMRFLDEDPWERLRTLKKYARRTPLQMLLRAQSLVGYQLYP
HHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
DDVVRAFIARAAANGIDIIRIFDALNDLRNMEVPVEAAKKEGVHVQGTVVYTISPVHTTE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEEECCCHHHH
HYLKTALELESMGVDSICIKDMAGLLAPFEAYKLVKLFKEKLHVPVQLHSHYIGGLAVGA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YLEAARAGVDVVDTASVPLAFGASQPPVETVVRALEGTPYDTGLDLNLLFEIARYFDDLR
HHHHHHCCCCEEECCCCCEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RELGYERGVTRITDMWVFQHQVPGGMISNLVSQLKEQKAADRINEVLAEIPRVRADLGYP
HHHCHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLVTPTSQIVGTQAVLNVLLGERYKMVPGEVKNYVRGLYGRPPAPISEEIRRLIIGDEEP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
IQGRPADILEPRLEEARREIGDLARNEDDVVAYAMFPQIARKFFEKRQQGQLRPGRQSLP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
SRGQDKAEAGGTAKMDLKDITQLIKALEETGITELNLESEGVKVMIRRGSGQGAAEIPAP
CCCCCHHHCCCCCEECHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
EIKTAAEVLATDGGAQETPVPAGDIIEVRAPMVGTFYRAPSPDAPPFVEVGTRVKAGQTL
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHEECCCCCCCCCHHHHCCEECCCCCH
CIIEAMKLMNELTAETGGQVVAILAENGQPVEYGQVLFQIKKD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEECC
>Mature Secondary Structure 
AVAEPAMGNVALALARVFLYGGGAVLSQLKITDTTLRDGHQSLWATRMTTADMLPIIEK
CCCCCCCCHHHHHHHHHHHHCCHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDSVGYHSLEVWGGATFDVCMRFLDEDPWERLRTLKKYARRTPLQMLLRAQSLVGYQLYP
HHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
DDVVRAFIARAAANGIDIIRIFDALNDLRNMEVPVEAAKKEGVHVQGTVVYTISPVHTTE
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEEEEECCCHHHH
HYLKTALELESMGVDSICIKDMAGLLAPFEAYKLVKLFKEKLHVPVQLHSHYIGGLAVGA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YLEAARAGVDVVDTASVPLAFGASQPPVETVVRALEGTPYDTGLDLNLLFEIARYFDDLR
HHHHHHCCCCEEECCCCCEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RELGYERGVTRITDMWVFQHQVPGGMISNLVSQLKEQKAADRINEVLAEIPRVRADLGYP
HHHCHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLVTPTSQIVGTQAVLNVLLGERYKMVPGEVKNYVRGLYGRPPAPISEEIRRLIIGDEEP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
IQGRPADILEPRLEEARREIGDLARNEDDVVAYAMFPQIARKFFEKRQQGQLRPGRQSLP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
SRGQDKAEAGGTAKMDLKDITQLIKALEETGITELNLESEGVKVMIRRGSGQGAAEIPAP
CCCCCHHHCCCCCEECHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
EIKTAAEVLATDGGAQETPVPAGDIIEVRAPMVGTFYRAPSPDAPPFVEVGTRVKAGQTL
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHEECCCCCCCCCHHHHCCEECCCCCH
CIIEAMKLMNELTAETGGQVVAILAENGQPVEYGQVLFQIKKD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]