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The map label for this gene is aegA [H]

Identifier: 83590360

GI number: 83590360

Start: 1562704

End: 1564095

Strand: Reverse

Name: aegA [H]

Synonym: Moth_1517

Alternate gene names: 83590360

Gene position: 1564095-1562704 (Counterclockwise)

Preceding gene: 83590361

Following gene: 83590359

Centisome position: 59.5

GC content: 60.13

Gene sequence:

>1392_bases
ATGCCATTAATCCCTAAGAAGACCCCTATGCCTTCCCAGGAACCCAGGGAGCGGATTCATAACTTCAACGAAGTAGCGCA
GGGTTATACCCGGGAAATGGCCCTGGCTGAGGCTCAGCGTTGCCTTCAGTGCAAAAAGGCCCCCTGCCGCCAGGGTTGCC
CGGTAGAAGTAGATATACCGGCCTTTATCGCCCGCTTGAAGGAGCAGGACTTTGACGGCGCCATTGCCAAGATCAAAGAA
AAGAATAACCTGCCGGCCATCTGCGGCCGGGTTTGCCCCCAGGAAAACCAGTGTGAAAAATTCTGCACCCTCGGCAAAAA
ACACGAGCCAGTGGCCATCGGTCGCCTGGAGCGCTTCCTGGCCGATTACCAGCTGGCCAAAGGCGAGACGGCCAGTAGCG
AAAAGGCGCCACCCAGCGGTTACAAAGTGGCGGTCATCGGTTCCGGGCCGGCCGGCCTGACGGCTGCTGCCGACCTGGCC
AGGATGGGACATCAGGTAACAGTCTTTGAGGCCCTCCATGTACCCGGGGGCGTGCTCATGTACGGTATTCCCGAGTTCCG
GTTGCCGAAAAGGATTGTTCAACAGGAGATAGATACTATTCGCCGTCTTGGGGTGGAGATCCGGACCAATGCCGTGGTAG
GCAAGCTGACTACGGTGGACGAGTTGCTGGAGAACGGTTACGACGCCGTCTTCATCGGTACGGGGGCCGGGTTGCCCCAC
TTTATGGGGATTCCCGGGGAGAACCTCCTGGGCGTTTACTCGGCCAATGAGTTTTTGACCCGGACCAACCTGATGAAGGC
CTACCTATTCCCCCGGTATGCTACTCCCATCAAGGTCGGGAAACGGGTGGCCGTCATCGGCGCCGGCAATGTAGCCATGG
ATGCCGCCCGTACAGCTTTGCGCCTGGGAGCCGAGGAATCCTATATCGTTTACCGCCGTTCGGCAGCGGAGATGCCAGCC
CGCAAGGAAGAAGTCGAGCACGCCGAGGAAGAAGGCGTCCAGTTCCGCCTCCTAACCAGTCCTGTGCGTATCCACGGCAA
CGACCAGGGCGTAGTTACAGGCATGACCTGCCAGCGCTTCGAACTGGGCGAACCCGATGCCTCCGGCCGGCGACGTCCGG
TGCCCATTCCCGGTTCTGAGTACGACATGGCGGTCGATACCGTCGTCATCGCCATCGGCCAGGGACCTAATCCCTTGGTC
CTGCGGACAACTCCGGGCCTGAAGCTCACAAGCAAGGGAACCATTGCCGCCGACGAGGCAACCGGTGCCACTTCCCGTAA
GGGCGTTTTTGCCGGCGGTGACATCGTCACCGGGGCGGCTACCGTCATCCTGGCCATGGGAGCCGGTAAAGCGGCCGCCC
GGGCCATTGACGCCTATCTACGGGAAAAATAA

Upstream 100 bases:

>100_bases
ACGGCCATGAGATTGACTGGCAACTGGCTTTACGACGGATGAACATGTACCGGGAAGAAGAGCAGGAAATTATTAAAGCC
CATGAGGGAGGTGGCTGCGG

Downstream 100 bases:

>100_bases
TATATAAAGGAGGGTATTTTTATGCCAGCCCAGATCCTCGACGGGAAAAAGATTGCTGCCGAAGTAAGGGCTGAAGTAAA
GGAGGAGGTATCCCGGTTAA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPLIPKKTPMPSQEPRERIHNFNEVAQGYTREMALAEAQRCLQCKKAPCRQGCPVEVDIPAFIARLKEQDFDGAIAKIKE
KNNLPAICGRVCPQENQCEKFCTLGKKHEPVAIGRLERFLADYQLAKGETASSEKAPPSGYKVAVIGSGPAGLTAAADLA
RMGHQVTVFEALHVPGGVLMYGIPEFRLPKRIVQQEIDTIRRLGVEIRTNAVVGKLTTVDELLENGYDAVFIGTGAGLPH
FMGIPGENLLGVYSANEFLTRTNLMKAYLFPRYATPIKVGKRVAVIGAGNVAMDAARTALRLGAEESYIVYRRSAAEMPA
RKEEVEHAEEEGVQFRLLTSPVRIHGNDQGVVTGMTCQRFELGEPDASGRRRPVPIPGSEYDMAVDTVVIAIGQGPNPLV
LRTTPGLKLTSKGTIAADEATGATSRKGVFAGGDIVTGAATVILAMGAGKAAARAIDAYLREK

Sequences:

>Translated_463_residues
MPLIPKKTPMPSQEPRERIHNFNEVAQGYTREMALAEAQRCLQCKKAPCRQGCPVEVDIPAFIARLKEQDFDGAIAKIKE
KNNLPAICGRVCPQENQCEKFCTLGKKHEPVAIGRLERFLADYQLAKGETASSEKAPPSGYKVAVIGSGPAGLTAAADLA
RMGHQVTVFEALHVPGGVLMYGIPEFRLPKRIVQQEIDTIRRLGVEIRTNAVVGKLTTVDELLENGYDAVFIGTGAGLPH
FMGIPGENLLGVYSANEFLTRTNLMKAYLFPRYATPIKVGKRVAVIGAGNVAMDAARTALRLGAEESYIVYRRSAAEMPA
RKEEVEHAEEEGVQFRLLTSPVRIHGNDQGVVTGMTCQRFELGEPDASGRRRPVPIPGSEYDMAVDTVVIAIGQGPNPLV
LRTTPGLKLTSKGTIAADEATGATSRKGVFAGGDIVTGAATVILAMGAGKAAARAIDAYLREK
>Mature_462_residues
PLIPKKTPMPSQEPRERIHNFNEVAQGYTREMALAEAQRCLQCKKAPCRQGCPVEVDIPAFIARLKEQDFDGAIAKIKEK
NNLPAICGRVCPQENQCEKFCTLGKKHEPVAIGRLERFLADYQLAKGETASSEKAPPSGYKVAVIGSGPAGLTAAADLAR
MGHQVTVFEALHVPGGVLMYGIPEFRLPKRIVQQEIDTIRRLGVEIRTNAVVGKLTTVDELLENGYDAVFIGTGAGLPHF
MGIPGENLLGVYSANEFLTRTNLMKAYLFPRYATPIKVGKRVAVIGAGNVAMDAARTALRLGAEESYIVYRRSAAEMPAR
KEEVEHAEEEGVQFRLLTSPVRIHGNDQGVVTGMTCQRFELGEPDASGRRRPVPIPGSEYDMAVDTVVIAIGQGPNPLVL
RTTPGLKLTSKGTIAADEATGATSRKGVFAGGDIVTGAATVILAMGAGKAAARAIDAYLREK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=472, Percent_Identity=32.2033898305085, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI237757300, Length=102, Percent_Identity=38.2352941176471, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=445, Percent_Identity=39.5505617977528, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1789606, Length=455, Percent_Identity=36.9230769230769, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI87082180, Length=444, Percent_Identity=38.7387387387387, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788468, Length=455, Percent_Identity=34.9450549450549, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI1789243, Length=430, Percent_Identity=35.8139534883721, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71984108, Length=463, Percent_Identity=33.0453563714903, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17570289, Length=476, Percent_Identity=28.9915966386555, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=34.1614906832298, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=484, Percent_Identity=31.198347107438, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI28574881, Length=480, Percent_Identity=31.0416666666667, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24665539, Length=480, Percent_Identity=31.0416666666667, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24665547, Length=480, Percent_Identity=31.0416666666667, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24665543, Length=480, Percent_Identity=31.0416666666667, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24640763, Length=461, Percent_Identity=32.7548806941432, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI18858217, Length=461, Percent_Identity=32.7548806941432, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 49846; Mature: 49715

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLIPKKTPMPSQEPRERIHNFNEVAQGYTREMALAEAQRCLQCKKAPCRQGCPVEVDIP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHH
AFIARLKEQDFDGAIAKIKEKNNLPAICGRVCPQENQCEKFCTLGKKHEPVAIGRLERFL
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
ADYQLAKGETASSEKAPPSGYKVAVIGSGPAGLTAAADLARMGHQVTVFEALHVPGGVLM
HHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEE
YGIPEFRLPKRIVQQEIDTIRRLGVEIRTNAVVGKLTTVDELLENGYDAVFIGTGAGLPH
ECCCCCCCHHHHHHHHHHHHHHCCCEEECCEEEEHHHHHHHHHHCCCCEEEEECCCCCCH
FMGIPGENLLGVYSANEFLTRTNLMKAYLFPRYATPIKVGKRVAVIGAGNVAMDAARTAL
HCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEECCCHHHHHHHHHH
RLGAEESYIVYRRSAAEMPARKEEVEHAEEEGVQFRLLTSPVRIHGNDQGVVTGMTCQRF
HCCCCCCEEEEECCHHCCCCHHHHHHHHHHCCCEEEEEECCEEEECCCCCEEECCEEEEE
ELGEPDASGRRRPVPIPGSEYDMAVDTVVIAIGQGPNPLVLRTTPGLKLTSKGTIAADEA
ECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCEEECCCCEEECCC
TGATSRKGVFAGGDIVTGAATVILAMGAGKAAARAIDAYLREK
CCCCCCCCEEECCCCHHHHHHEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLIPKKTPMPSQEPRERIHNFNEVAQGYTREMALAEAQRCLQCKKAPCRQGCPVEVDIP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHH
AFIARLKEQDFDGAIAKIKEKNNLPAICGRVCPQENQCEKFCTLGKKHEPVAIGRLERFL
HHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
ADYQLAKGETASSEKAPPSGYKVAVIGSGPAGLTAAADLARMGHQVTVFEALHVPGGVLM
HHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCEEE
YGIPEFRLPKRIVQQEIDTIRRLGVEIRTNAVVGKLTTVDELLENGYDAVFIGTGAGLPH
ECCCCCCCHHHHHHHHHHHHHHCCCEEECCEEEEHHHHHHHHHHCCCCEEEEECCCCCCH
FMGIPGENLLGVYSANEFLTRTNLMKAYLFPRYATPIKVGKRVAVIGAGNVAMDAARTAL
HCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEECCCHHHHHHHHHH
RLGAEESYIVYRRSAAEMPARKEEVEHAEEEGVQFRLLTSPVRIHGNDQGVVTGMTCQRF
HCCCCCCEEEEECCHHCCCCHHHHHHHHHHCCCEEEEEECCEEEECCCCCEEECCEEEEE
ELGEPDASGRRRPVPIPGSEYDMAVDTVVIAIGQGPNPLVLRTTPGLKLTSKGTIAADEA
ECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCEEECCCCEEECCC
TGATSRKGVFAGGDIVTGAATVILAMGAGKAAARAIDAYLREK
CCCCCCCCEEECCCCHHHHHHEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]